Kent D. Chapman
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View article: The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid dropletsy
The jojoba lipid droplet protein LDAP1 facilitates the packaging of wax esters into lipid dropletsy Open
Jojoba (Simmondsia chinensis) is a desert shrub with an unusual capacity to store liquid wax esters (WEs) in its seeds instead of triacylglycerols (TAGs) like most oilseed crops. To examine the factors that are important for WE compartment…
View article: Alkali niobium borogermanosilicate optical fiber derived via the molten core method [Invited]
Alkali niobium borogermanosilicate optical fiber derived via the molten core method [Invited] Open
Herein, multicomponent glass optical fibers are produced using the molten core fiber fabrication method. A pre-melted glass of 30 K 2 O-20 Nb 2 O 5 -50 GeO 2 is used as a starting core material along with a low-alkali borosilicate (Duran) …
View article: LIPID DROPLET PROTEIN OF SEEDS is involved in the control of lipid droplet size in Arabidopsis seeds and seedlings
LIPID DROPLET PROTEIN OF SEEDS is involved in the control of lipid droplet size in Arabidopsis seeds and seedlings Open
In oilseeds, energy-rich carbon is stored as triacylglycerols in organelles called lipid droplets (LDs). While several of the major biogenetic proteins involved in LD formation have been identified, the full repertoire of LD proteins and t…
View article: Identification and Characterization of Lipid Droplet-Associated Protein (LDAP) Isoforms from Tung Tree (Vernicia fordii)
Identification and Characterization of Lipid Droplet-Associated Protein (LDAP) Isoforms from Tung Tree (Vernicia fordii) Open
Lipid droplets (LDs) are cytoplasmic organelles responsible primarily for the storage of neutral lipids, such as triacyclglycerols (TAGs). Derived from the endoplasmic reticulum bilayer, LDs are composed of a hydrophobic lipid core encased…
View article: Targeted modulation of pennycress lipid droplet proteins impacts droplet morphology and seed oil content
Targeted modulation of pennycress lipid droplet proteins impacts droplet morphology and seed oil content Open
SUMMARY Lipid droplets (LDs) are unusual organelles that have a phospholipid monolayer surface and a hydrophobic matrix. In oilseeds, this matrix is nearly always composed of triacylglycerols (TGs) for efficient storage of carbon and energ…
View article: <i>Ex vivo</i> lipidomics reveal monoacylglycerols as substrates for a fatty acid amide hydrolase in the legume <i>Medicago truncatula</i>
<i>Ex vivo</i> lipidomics reveal monoacylglycerols as substrates for a fatty acid amide hydrolase in the legume <i>Medicago truncatula</i> Open
Fatty acid amide hydrolase (FAAH) is a conserved hydrolase in eukaryotes with promiscuous activity toward a range of acylamide substrates. The native substrate repertoire for FAAH has just begun to be explored in plant systems outside the …
View article: Abstract 2309 Using plants as bio-factories to produce high-value fungal-derived medicines
Abstract 2309 Using plants as bio-factories to produce high-value fungal-derived medicines Open
The antibiotic penicillin G, and the immunosuppressant mycophenolic acid (MPA), are critical and widely used medicines with high economical values. They are produced by fungi and their biosynthetic pathways have been well described. Our go…
View article: Imaging plant metabolism <i>in situ</i>
Imaging plant metabolism <i>in situ</i> Open
Mass spectrometry imaging (MSI) has emerged as an invaluable analytical technique for investigating the spatial distribution of molecules within biological systems. In the realm of plant science, MSI is increasingly employed to explore met…
View article: Seeing plants as never before
Seeing plants as never before Open
Summary Imaging has long supported our ability to understand the inner life of plants, their development, and response to a dynamic environment. While optical microscopy remains the core tool for imaging, a suite of novel technologies is n…
View article: Effects of cultivar and nitrogen application rate on lint, seed, oil, and protein yields of field‐grown cotton
Effects of cultivar and nitrogen application rate on lint, seed, oil, and protein yields of field‐grown cotton Open
Cotton ( Gossypium hirsutum L.) is not only the dominant fiber crop grown worldwide, but it is also an important source of plant‐based oil and protein. Previous research has documented a significant effect of cotton cultivar and nitrogen a…
View article: Methods for elevating fat/oil content in plants
Methods for elevating fat/oil content in plants Open
In some embodiments, the present invention provides a method of elevating lipid content in vegetative (non-seed) plant or algae cells, plant tissues, or whole plants by genetically modifying the plant or algae to express a lipid droplet-as…
View article: Method for enhancing amidohydrolase activity of fatty acid amide hydrolase
Method for enhancing amidohydrolase activity of fatty acid amide hydrolase Open
A method for enhancing amidohydrolase activity of Fatty Acid Amide Hydrolase (FAAH) is disclosed. The method comprising administering a phenoxyacylethanolamide that causes the enhanced activity. The enhanced activity can have numerous effe…
View article: Analysis of Pectin-derived Monosaccharides from <em>Arabidopsis</em> Using GC–MS
Analysis of Pectin-derived Monosaccharides from <em>Arabidopsis</em> Using GC–MS Open
Pectin is a complex polysaccharide present in the plant cell wall, whose composition is constantly remodelled to adapt to environmental or developmental changes. Mutants with altered pectin composition have been reported to exhibit altered…
View article: Quantification of <em>Botrytis cinerea</em> Growth in <em>Arabidopsis thaliana</em>
Quantification of <em>Botrytis cinerea</em> Growth in <em>Arabidopsis thaliana</em> Open
Yield losses attributed to plant pathogens pose a serious threat to plant productivity and food security. Botrytis cinerea is one of the most devastating plant pathogens, infecting a wide array of plant species; it has also been establishe…
View article: Fatty acid amide hydrolase and 9-lipoxygenase modulate cotton seedling growth by ethanolamide oxylipin levels
Fatty acid amide hydrolase and 9-lipoxygenase modulate cotton seedling growth by ethanolamide oxylipin levels Open
Polyunsaturated N-acylethanolamines (NAEs) can be hydrolyzed by fatty acid amide hydrolase (FAAH) or oxidized by lipoxygenase (LOX). In Arabidopsis (Arabidopsis thaliana), the 9-LOX product of linoleoylethanolamide, namely, 9-hydroxy linol…
View article: Lipid Droplets: Packing Hydrophobic Molecules Within the Aqueous Cytoplasm
Lipid Droplets: Packing Hydrophobic Molecules Within the Aqueous Cytoplasm Open
Lipid droplets, also known as oil bodies or lipid bodies, are plant organelles that compartmentalize neutral lipids as a hydrophobic matrix covered by proteins embedded in a phospholipid monolayer. Some of these proteins have been known fo…
View article: A lipidomics platform to analyze the fatty acid compositions of non-polar and polar lipid molecular species from plant tissues: Examples from developing seeds and seedlings of pennycress (Thlaspi arvense)
A lipidomics platform to analyze the fatty acid compositions of non-polar and polar lipid molecular species from plant tissues: Examples from developing seeds and seedlings of pennycress (Thlaspi arvense) Open
The lipidome comprises the total content of molecular species of each lipid class, and is measured using the analytical techniques of lipidomics. Many liquid chromatography-mass spectrometry (LC-MS) methods have previously been described t…
View article: N-acylethanolamide metabolizing enzymes are upregulated in human neural progenitor-derived neurons exposed to sub-lethal oxidative stress
N-acylethanolamide metabolizing enzymes are upregulated in human neural progenitor-derived neurons exposed to sub-lethal oxidative stress Open
N -acyl amides (NAAs) are a class of lipids that consist of an acyl group N-linked to an amino acid, neurotransmitter, taurine or ethanolamide group ( N -acylethanolamines or NAEs) and include some endocannabinoids (eCB) such as anandamide…
View article: Finding new friends and revisiting old ones – how plant lipid droplets connect with other subcellular structures
Finding new friends and revisiting old ones – how plant lipid droplets connect with other subcellular structures Open
Summary The number of described contact sites between different subcellular compartments and structures in eukaryotic cells has increased dramatically in recent years and, as such, has substantially reinforced the well‐known premise that t…
View article: Effective Mechanisms for Improving Seed Oil Production in Pennycress (Thlaspi arvense L.) Highlighted by Integration of Comparative Metabolomics and Transcriptomics
Effective Mechanisms for Improving Seed Oil Production in Pennycress (Thlaspi arvense L.) Highlighted by Integration of Comparative Metabolomics and Transcriptomics Open
Pennycress is a potentially lucrative biofuel crop due to its high content of long-chain unsaturated fatty acids, and because it uses non-conventional pathways to achieve efficient oil production. However, metabolic engineering is required…
View article: Enhanced seedling growth by 3‐<i>n</i>‐pentadecylphenolethanolamide is mediated by fatty acid amide hydrolases in upland cotton (<scp><i>Gossypium hirsutum</i></scp> L.)
Enhanced seedling growth by 3‐<i>n</i>‐pentadecylphenolethanolamide is mediated by fatty acid amide hydrolases in upland cotton (<span><i>Gossypium hirsutum</i></span> L.) Open
Fatty acid amide hydrolase (FAAH) is a conserved amidase that is known to modulate the levels of endogenous N ‐acylethanolamines (NAEs) in both plants and animals. The activity of FAAH is enhanced in vitro by synthetic phenoxyacylethanolam…
View article: Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism
Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism Open
Oilseed rape ( Brassica napus ) is an important crop that is cultivated for the oil (mainly triacylglycerol; TAG) it produces in its seeds. TAG synthesis is controlled mainly by key enzymes in the Kennedy pathway, such as glycerol 3-phosph…
View article: Better together: Protein partnerships for lineage-specific oil accumulation
Better together: Protein partnerships for lineage-specific oil accumulation Open
Plant-derived oils are a major agricultural product that exist in both ubiquitous forms such as common vegetable oils and in specialized forms such as castor oil and coconut oil. These specialized oils are the result of lineage-specific me…
View article: A glossary of plant cell structures: Current insights and future questions
A glossary of plant cell structures: Current insights and future questions Open
In this glossary of plant cell structures, we asked experts to summarize a present-day view of plant organelles and structures, including a discussion of outstanding questions. In the following short reviews, the authors discuss the comple…
View article: LDIP cooperates with SEIPIN and LDAP to facilitate lipid droplet biogenesis in Arabidopsis
LDIP cooperates with SEIPIN and LDAP to facilitate lipid droplet biogenesis in Arabidopsis Open
Cytoplasmic lipid droplets (LDs) are evolutionarily conserved organelles that store neutral lipids and play critical roles in plant growth, development, and stress responses. However, the molecular mechanisms underlying their biogenesis at…
View article: CRISPR/Cas9-Induced fad2 and rod1 Mutations Stacked With fae1 Confer High Oleic Acid Seed Oil in Pennycress (Thlaspi arvense L.)
CRISPR/Cas9-Induced fad2 and rod1 Mutations Stacked With fae1 Confer High Oleic Acid Seed Oil in Pennycress (Thlaspi arvense L.) Open
Pennycress ( Thlaspi arvense L.) is being domesticated as an oilseed cash cover crop to be grown in the off-season throughout temperate regions of the world. With its diploid genome and ease of directed mutagenesis using molecular approach…
View article: Arabidopsis thaliana EARLY RESPONSIVE TO DEHYDRATION 7 Localizes to Lipid Droplets via Its Senescence Domain
Arabidopsis thaliana EARLY RESPONSIVE TO DEHYDRATION 7 Localizes to Lipid Droplets via Its Senescence Domain Open
Lipid droplets (LDs) are neutral-lipid-containing organelles found in all kingdoms of life and are coated with proteins that carry out a vast array of functions. Compared to mammals and yeast, relatively few LD proteins have been identifie…