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View article: Antibodies targeting the Crimean-Congo Hemorrhagic Fever Virus nucleoprotein protect via TRIM21
Antibodies targeting the Crimean-Congo Hemorrhagic Fever Virus nucleoprotein protect via TRIM21 Open
Crimean-Congo Hemorrhagic Fever Virus (CCHFV) is a negative-sense RNA virus spread by Hyalomma genus ticks across Europe, Asia, and Africa. CCHF disease begins as a non-specific febrile illness which may progress into a severe hemorrhagic …
View article: TRIM7 Restricts Coxsackievirus and Norovirus Infection by Detecting the C-Terminal Glutamine Generated by 3C Protease Processing
TRIM7 Restricts Coxsackievirus and Norovirus Infection by Detecting the C-Terminal Glutamine Generated by 3C Protease Processing Open
TRIM7 catalyzes the ubiquitination of multiple substrates with unrelated biological functions. This cross-reactivity is at odds with the specificity usually displayed by enzymes, including ubiquitin ligases. Here we show that TRIM7′s extre…
View article: TRIM7 restricts Coxsackievirus and norovirus infection by detecting the C-terminal glutamine generated by 3C protease processing
TRIM7 restricts Coxsackievirus and norovirus infection by detecting the C-terminal glutamine generated by 3C protease processing Open
TRIM7 catalyses the ubiquitination of multiple substrates with unrelated biological functions. This cross-reactivity is at odds with the specificity usually displayed by enzymes, including ubiquitin ligases. Here we show that TRIM7’s extre…
View article: Trim-Away degrades lysine-less substrates without requiring ligase autoubiquitination
Trim-Away degrades lysine-less substrates without requiring ligase autoubiquitination Open
TRIM proteins are the largest family of E3 ligases in mammals. They include the intracellular antibody receptor TRIM21, which is responsible for mediating targeted protein degradation during Trim-Away. Despite their importance, the ubiquit…
View article: SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase
SUMOylation stabilizes sister kinetochore biorientation to allow timely anaphase Open
During mitosis, sister chromatids attach to microtubules from opposite poles, called biorientation. Sister chromatid cohesion resists microtubule forces, generating tension, which provides the signal that biorientation has occurred. How te…
View article: RING domains act as both substrate and enzyme in a catalytic arrangement to drive self-anchored ubiquitination
RING domains act as both substrate and enzyme in a catalytic arrangement to drive self-anchored ubiquitination Open
Attachment of ubiquitin (Ub) to proteins is one of the most abundant and versatile of all posttranslational modifications and affects outcomes in essentially all physiological processes. RING E3 ligases target E2 Ub-conjugating enzymes to …
View article: Substrate-induced clustering activates Trim-Away of pathogens and proteins
Substrate-induced clustering activates Trim-Away of pathogens and proteins Open
SUMMARY Trim-Away is a powerful new technology that exploits off-the-shelf antibodies and the E3 RING ligase and cytosolic antibody receptor TRIM21 to carry out rapid protein depletion. How TRIM21 is catalytically-activated upon substrate …
View article: SUMOylation targets shugoshin to stabilize sister kinetochore biorientation
SUMOylation targets shugoshin to stabilize sister kinetochore biorientation Open
The accurate segregation of chromosomes during mitosis relies on the attachment of sister chromatids to microtubules from opposite poles, called biorientation. Sister chromatid cohesion resists microtubule forces, generating tension which …
View article: A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes
A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes Open
A new phase in egg biology Chromosome segregation typically requires centrosomes, which generate the microtubule spindle. However, mammalian eggs build a spindle and segregate chromosomes without centrosomes. How acentrosomal spindles are …
View article: Targeting Of Nat10 Enhances Healthspan In A Mouse Model Of Human Accelerated Aging Syndrome
Targeting Of Nat10 Enhances Healthspan In A Mouse Model Of Human Accelerated Aging Syndrome Open
Hutchinson-Gilford Progeria Syndrome (HGPS) is a rare but devastating genetic disease characterized by segmental premature aging, with cardiovascular disease being the main cause of death. Cells from HGPS patients accumulate progerin, a pe…
View article: Cytosolic Fc receptor TRIM21 inhibits seeded tau aggregation
Cytosolic Fc receptor TRIM21 inhibits seeded tau aggregation Open
Significance The mammalian cell cytoplasm contains numerous proteins with direct antimicrobial activity. Although these have been extensively studied in the context of viral and bacterial infection, it is unknown whether pathogenic self-pr…
View article: A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes
A three-step MTOC fragmentation mechanism facilitates bipolar spindle assembly in mouse oocytes Open
Assembly of a bipolar microtubule spindle is essential for accurate chromosome segregation. In somatic cells, spindle bipolarity is determined by the presence of exactly two centrosomes. Remarkably, mammalian oocytes do not contain canonic…