Deborah Jaffey
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View article: A novel statistical methodology for quantifying the spatial arrangements of axons in peripheral nerves
A novel statistical methodology for quantifying the spatial arrangements of axons in peripheral nerves Open
A thorough understanding of the neuroanatomy of peripheral nerves is required for a better insight into their function and the development of neuromodulation tools and strategies. In biophysical modeling, it is commonly assumed that the co…
View article: Vagal preganglionic axons arborize in the myenteric plexus into two types: nitrergic and non-nitrergic postganglionic motor pools?
Vagal preganglionic axons arborize in the myenteric plexus into two types: nitrergic and non-nitrergic postganglionic motor pools? Open
Vagal preganglionic neurons innervate myenteric ganglia. These autonomic efferents are distributed so densely within the ganglia that it has been impractical to track individual vagal axons through the myenteric plexus with tracer labeling…
View article: A novel statistical methodology for quantifying the spatial arrangements of axons in peripheral nerves
A novel statistical methodology for quantifying the spatial arrangements of axons in peripheral nerves Open
A thorough understanding of the neuroanatomy of peripheral nerves is required for a better insight into their function and the development of neuromodulation tools and strategies. In biophysical modeling, it is commonly assumed that the co…
View article: High resolution labeling of vagal efferent fibers using Dextran-Biotin with counterstaining v2
High resolution labeling of vagal efferent fibers using Dextran-Biotin with counterstaining v2 Open
This protocol describes the methods used to trace and enable morphometric quantification of preganglionic efferent neurites in the rat stomach and duodenum. A mixture of dextran conjugates was injected into the dorsal motor nucleus of the …
View article: Collection of human vagal tissue samples for TEM imaging v2
Collection of human vagal tissue samples for TEM imaging v2 Open
This protocol describes the methods used to generate samples of human vagus tissue suitable for TEM imaging.
View article: Collection of human vagal tissue samples for TEM imaging v1
Collection of human vagal tissue samples for TEM imaging v1 Open
This protocol describes the methods used to generate samples of human vagus tissue suitable for TEM imaging.
View article: Human organ donor-derived vagus nerve biopsies allow for well-preserved ultrastructure and high-resolution mapping of myelinated and unmyelinated fibers
Human organ donor-derived vagus nerve biopsies allow for well-preserved ultrastructure and high-resolution mapping of myelinated and unmyelinated fibers Open
The vagus nerve provides motor, sensory, and autonomic innervation of multiple organs, and electrical vagus nerve stimulation (VNS) provides an adjunctive treatment option for e.g. medication-refractory epilepsy and treatment-resistant dep…
View article: Protocol for chronic implantation of patch electrodes on the gastric muscle wall of the rat v1
Protocol for chronic implantation of patch electrodes on the gastric muscle wall of the rat v1 Open
Electrical stimulation is a potential therapy for gastric disorders. Here we describe our surgical procedure for the implantation of patch electrodes on the muscle wall of the stomach of both male and female rats for use in chronic studies…
View article: Collection of rat vagal tissue samples for TEM imaging v1
Collection of rat vagal tissue samples for TEM imaging v1 Open
This protocol describes the methods used to generate samples of rat vagus tissue suitable for TEM imaging.
View article: High-throughput segmentation of unmyelinated axons by deep learning
High-throughput segmentation of unmyelinated axons by deep learning Open
Axonal characterizations of connectomes in healthy and disease phenotypes are surprisingly incomplete and biased because unmyelinated axons, the most prevalent type of fibers in the nervous system, have largely been ignored as their quanti…
View article: Automatic assessment of human gastric motility and emptying from dynamic 3D magnetic resonance imaging
Automatic assessment of human gastric motility and emptying from dynamic 3D magnetic resonance imaging Open
Background Time‐sequenced magnetic resonance imaging (MRI) of the stomach is an emerging technique for non‐invasive assessment of gastric emptying and motility. However, an automated and systematic image processing pipeline for analyzing d…
View article: Stomach serosal arteries distinguish gastric regions of the rat
Stomach serosal arteries distinguish gastric regions of the rat Open
Because the stomach in situ has few distinctive surface features and changes shape dramatically with food intake, we have used micro‐CT imaging combined with two distinct contrast agents to (1) characterize the pattern of arteries, potenti…
View article: Stomach serosal arteries distinguish gastric regions of the rat
Stomach serosal arteries distinguish gastric regions of the rat Open
Because the stomach in situ has few distinctive surface features and changes shape dramatically with food intake, we have used microCT imaging to (1) characterize the pattern of arteries, potential landmarks, on the stomach wall and (2) ev…
View article: Stomach region stimulated determines effects on duodenal motility in rats
Stomach region stimulated determines effects on duodenal motility in rats Open
Gastric electrical stimulation (GES) is used clinically to promote proximal GI emptying and motility. In acute experiments, we measured duodenal motor responses elicited by GES applied at 141 randomly chosen electrode sites on the stomach …
View article: Automatic Assessment of Human Gastric Motility and Emptying from Dynamic 3D Magnetic Resonance Imaging
Automatic Assessment of Human Gastric Motility and Emptying from Dynamic 3D Magnetic Resonance Imaging Open
Background Time-sequenced magnetic resonance imaging (MRI) of the stomach is an emerging technique for non-invasive assessment of gastric emptying and motility. However, an automated and systematic image processing pipeline for analyzing d…
View article: Micro-CT imaging of rat stomach vasculature v1
Micro-CT imaging of rat stomach vasculature v1 Open
This protocol describes how to prepare a rat stomach for micro-CT imaging of the vasculature. A lead-containing silicone-based curable material is injected into the heart at perfusion and flows through the vasculature and cures in place. S…
View article: Micro-CT imaging of iodine-stained rat stomach v1
Micro-CT imaging of iodine-stained rat stomach v1 Open
This protocol describes how to prepare a rat stomach for micro-CT imaging to enable a 3D description of the organ. Iodine is used to stain the tissue post-perfusion. By varying the meal size and the delay time between feeding and perfusion…
View article: Vagal innervation of the stomach reassessed: brain−gut connectome uses smart terminals
Vagal innervation of the stomach reassessed: brain−gut connectome uses smart terminals Open
Brain−gut neural communications have long been considered limited because of conspicuous numerical mismatches. The vagus, the parasympathetic nerve connecting brain and gut, contains thousands of axons, whereas the gastrointestinal (GI) tr…
View article: High resolution labeling of vagal efferent fibers using Dextran-Biotin with counterstaining v1
High resolution labeling of vagal efferent fibers using Dextran-Biotin with counterstaining v1 Open
This protocol describes the methods used to trace and enable morphometric quantification of preganglionic efferent neurites in the rat stomach. A mixture of dextran conjugates was injected into the dorsal motor nucleus of the vagal nerve (…