D.K. Berg
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View article: Using extended growth of cattle embryos in culture to gain insights into bovine developmental events on embryonic days 8 to 10
Using extended growth of cattle embryos in culture to gain insights into bovine developmental events on embryonic days 8 to 10 Open
We have previously described an extended embryo culture system, based on uterine composition, growth factors and the cell culture additive B27, for growing cattle embryos in vitro beyond embryonic day 7. Here, extended in vitro embryos are…
View article: Metabolomic evolution of the postpartum dairy cow uterus
Metabolomic evolution of the postpartum dairy cow uterus Open
High rates of early pregnancy loss are a critical issue in dairy herds, particularly in seasonal, grazing systems. Components of the uterine luminal fluid (ULF), on which the early embryo depends for sustenance and growth, partly determine…
View article: The first week following insemination is the period of major pregnancy failure in pasture-grazed dairy cows
The first week following insemination is the period of major pregnancy failure in pasture-grazed dairy cows Open
A 60% pregnancy success for inseminations is targeted to optimize production efficiency for dairy cows within a seasonal, pasture-grazed system. Routine measures of pregnancy success are widely available but are limited, in practice, to a …
View article: Proteomics and metabolomics in cow fertility: a systematic review
Proteomics and metabolomics in cow fertility: a systematic review Open
Cow subfertility is a multi-factorial problem in many countries which is only starting to be unravelled. Molecular biology can provide a substantial source of insight into its causes and potential solutions, particularly through large scal…
View article: On the enigmatic disappearance of Rauber’s layer
On the enigmatic disappearance of Rauber’s layer Open
Significance Trophoblast contributes to mammalian placenta formation, but it also has an earlier function in gastrulation induction within the embryonic epiblast. We show here that gastrulation induction occurs ectopically if the central a…
View article: A mathematical model of in vivo bovine blastocyst developmental to gestational Day 15
A mathematical model of in vivo bovine blastocyst developmental to gestational Day 15 Open
Bovine embryo growth involves a complex interaction between the developing embryo and the growth-promoting potential of the uterine environment. We have previously established links between embryonic factors (embryo stage, embryo gene expr…
View article: Combining genetic and physiological data to identify predictors of lifetime reproductive success and the effect of selection on these predictors on underlying fertility traits
Combining genetic and physiological data to identify predictors of lifetime reproductive success and the effect of selection on these predictors on underlying fertility traits Open
Fertility of the dairy cow relies on complex interactions between genetics, physiology, and management. Mathematical modeling can combine a range of information sources to facilitate informed predictions of cow fertility in scenarios that …
View article: A mathematical model of the interaction between bovine blastocyst developmental stage and progesterone-stimulated uterine factors on differential embryonic development observed on Day 15 of gestation
A mathematical model of the interaction between bovine blastocyst developmental stage and progesterone-stimulated uterine factors on differential embryonic development observed on Day 15 of gestation Open
A complex interaction between the developing bovine embryo and the growth potential of the uterine milieu it inhabits results in an embryo capable of developing past the maternal recognition stage and on to a successful pregnancy. Previous…
View article: Morphological and Gene Expression Changes in Cattle Embryos from Hatched Blastocyst to Early Gastrulation Stages after Transfer of In Vitro Produced Embryos
Morphological and Gene Expression Changes in Cattle Embryos from Hatched Blastocyst to Early Gastrulation Stages after Transfer of In Vitro Produced Embryos Open
A detailed morphological staging system for cattle embryos at stages following blastocyst hatching and preceding gastrulation is presented here together with spatiotemporal mapping of gene expression for BMP4, BRACHYURY, CERBERUS1 (CER1), …