Deepesh Kumar Gupta
YOU?
Author Swipe
View article: BACH2 regulates T cell lineage states to overcome dysfunction driven by tonic CAR signaling
BACH2 regulates T cell lineage states to overcome dysfunction driven by tonic CAR signaling Open
Like T cell receptors (TCRs), chimeric antigen receptors (CARs) trigger potent intracellular signaling upon engagement with antigen, a process which initiates cell state transitions that direct T cell functionality. Unlike TCRs, CARs have …
Prevalence of Periodontitis among Smoking and Smokeless Tobacco Consumers in the Tribal Population of Central India: A Cross-sectional Study Open
Introduction: Diminished ovarian reserve (DOR) is directly linked to female infertility and a poor response to ovarian stimulation. This study’s objective was to compare the effects of melatonin (MT) and dehydroepiandrosterone (DHEA) on ke…
Relationship between Smile Type and Periodontal Phenotype: A Clinical Cross-sectional Study Open
Objectives: To assess the relationship between smile category and periodontal makeup. Materials and Methods: A total of 58 participants who were at least 18 years old were examined clinically and through photography. The parameters that we…
View article: BACH2 regulates T cell lineage states to overcome dysfunction driven by tonic CAR signaling
BACH2 regulates T cell lineage states to overcome dysfunction driven by tonic CAR signaling Open
Nearly all chimeric antigen receptors (CARs) initiate intracellular signaling in the absence of antigen, referred to as “tonic signaling”. Tonic signaling of CARs containing the CD28 costimulatory domain has been shown to drive T cell exha…
View article: Comparative evaluation of marginal fit accuracy of two different designs of endocrown manufactured through CAD-CAM system: An in vitro study
Comparative evaluation of marginal fit accuracy of two different designs of endocrown manufactured through CAD-CAM system: An in vitro study Open
Aims: To compare the accuracy of marginal fit of CAD-CAM endocrown with two different preparation forms, i.e., endocrown with ferrule and endocrown without ferrule. Settings and Design: Various studies have opined on the impact of preparat…
View article: Cilia dysfunction in the lateral ventricles after neonatal intraventricular hemorrhage does not lead to functional changes in cilia-based CSF flow networks
Cilia dysfunction in the lateral ventricles after neonatal intraventricular hemorrhage does not lead to functional changes in cilia-based CSF flow networks Open
Intraventricular hemorrhage (IVH) has long been thought to lead to motile cilia dysfunction whereby intraventricular blood breakdown products damage and slough cilia from the ependymal wall. However, specifically how IVH may affect cilia d…
View article: Supplementary Figure 4 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors
Supplementary Figure 4 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors Open
Supplementary Figure 4 | Names and amino acid changes of miTCR variant panel. PD insertions are highlighted in blue.
View article: Supplementary Figure 3 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors
Supplementary Figure 3 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors Open
Supplementary Figure 3 | a, Predicted structures of miTCR1 and miTCR2 α and β chains overlaid on resolved structures of TCR α and β chains. b, Overlay of five highest-ranking predictions for miTCR1 and miTCR2 variable-constant chain interf…
View article: Supplementary Figure 7 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors
Supplementary Figure 7 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors Open
Supplementary Figure 7 | a, Overlay of predicted mut031 α chain variable region and resolved FMC63 variable chain structure. Modeling performed using AlphaFold2. b, Memory lineages of human T cells engineered to express miTCR1 WT or mut035…
View article: Supplementary Figure 6 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors
Supplementary Figure 6 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors Open
Supplementary Figure 6 | a-b, Cytotoxicity of a, miTCR1 and b, miTCR2 variants against Nalm6. c-d, Long-term flow cytometry-based cytotoxicity of lead c, miTCR1 and d, miTCR2 variants. e, Quantification of cytokines in supernatants 24h aft…
View article: Data from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors
Data from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors Open
Chimeric antigen receptor (CAR)–based therapies have pioneered synthetic cellular immunity but remain limited in their long-term efficacy. Emerging data suggest that dysregulated CAR-driven T-cell activation causes T-cell dysfunction and t…
View article: Supplementary Figure 1 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors
Supplementary Figure 1 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors Open
Supplementary Figure 1 | a-b, Expression of NFAT and NFκB in reporter Jurkat cells expressing CARs or miTCRs in response to a, CD19+ Nalm6 or CD19- Molm14. b, Representative flow cytometry plot of TCR constant chain expression after dual T…
View article: Supplementary Figure 2 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors
Supplementary Figure 2 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors Open
Supplementary Figure 2 | a-d, Expression of a, TCR constant chains and mCherry transduction marker, b, FMC63 and mCherry transduction marker, c, FMC63 and TCR constant chains and d, CD3ε and TCR constant chains in primary human T cells eng…
View article: Supplementary Figure 8 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors
Supplementary Figure 8 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors Open
Supplementary Figure 8 | a, Cytotoxicity, as measured by fold change in Nalm6 growth during co-culture, and b, expansion of human T cells expressing transgenic TCR 1G4, 19/BBζ or 19/28ζ CAR against Nalm6WT or Nalm6triple. c-d, Endogenous e…
View article: Supplementary Figure 5 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors
Supplementary Figure 5 from Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors Open
Supplementary Figure 5 | a-b, Expression of TCR constant chains on the surface of a, Jurkat and b, human T cells transduced with miTCR variants. c-d, Comparison of surface TCR expression in miTCR1 and miTCR2 variant engineered c, Jurkats a…
Transcriptional analysis of primary ciliary dyskinesia airway cells reveals a dedicated cilia glutathione pathway Open
Primary ciliary dyskinesia (PCD) is a genetic condition that results in dysmotile cilia. The repercussions of cilia dysmotility and gene variants on the multiciliated cell remain poorly understood. We used single-cell RNA-Seq, proteomics, …
View article: Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors
Rational Protein Engineering to Enhance MHC-Independent T-cell Receptors Open
Chimeric antigen receptor (CAR)–based therapies have pioneered synthetic cellular immunity but remain limited in their long-term efficacy. Emerging data suggest that dysregulated CAR-driven T-cell activation causes T-cell dysfunction and t…
View article: Ciliopathy patient variants reveal organelle-specific functions for TUBB4B in axonemal microtubules
Ciliopathy patient variants reveal organelle-specific functions for TUBB4B in axonemal microtubules Open
Tubulin, one of the most abundant cytoskeletal building blocks, has numerous isotypes in metazoans encoded by different conserved genes. Whether these distinct isotypes form cell type– and context-specific microtubule structures is poorly …
Rational protein engineering to enhance MHC-independent T cell receptors Open
Chimeric antigen receptor (CAR)-based therapies have pioneered synthetic cellular immunity against cancer, however remain limited in their scope and long-term efficacy. Emerging data suggest that dysregulated CAR-driven T cell activation c…
To Evaluate the Effect of Vitamin B Complex on Wound Healing – A Clinical and Microbiological Study Open
A BSTRACT Background: The purpose of this study is to explore the effect of vitamin B complex supplementation following periodontal flap surgery on clinical and microbiological parameters. Materials and Methods: A randomized controlled tri…
Sterilisation of Dental Implant Surgical Instruments, Office Area and Implant Components: A Narrative Review Open
Implant surgical procedures require meticulous attention to sterilisation protocols to prevent infections and ensure successful outcomes. Various sterilisation methods, such as steam sterilisation, ethylene oxide sterilisation and hydrogen…
View article: The effect of Dnaaf5 gene dosage on primary ciliary dyskinesia phenotypes
The effect of Dnaaf5 gene dosage on primary ciliary dyskinesia phenotypes Open
DNAAF5 is a dynein motor assembly factor associated with the autosomal heterogenic recessive condition of motile cilia, primary ciliary dyskinesia (PCD). The effects of allele heterozygosity on motile cilia function are unknown. We used CR…
View article: The effect of <i>Dnaaf5</i> gene dosage on primary ciliary dyskinesia phenotypes
The effect of <i>Dnaaf5</i> gene dosage on primary ciliary dyskinesia phenotypes Open
DNAAF5 is a dynein motor assembly factor associated with the autosomal heterogenic recessive condition of motile cilia, primary ciliary dyskinesia (PCD). The effects of allele heterozygosity on motile cilia function are unknown. We used CR…
Contribution of integrin adhesion to cytokinetic abscission and genomic integrity Open
Recent research shows that integrin-mediated adhesion contributes to the regulation of cell division at two key steps: the formation of the mitotic spindle at the mitotic entry and the final cytokinetic abscission at the mitotic exit. Fail…
View article: <i>TUBB4B</i> variants specifically impact ciliary function, causing a ciliopathic spectrum
<i>TUBB4B</i> variants specifically impact ciliary function, causing a ciliopathic spectrum Open
Cilia are small microtubule-based structures found on the surface of most mammalian cells, which have key sensory and sometimes motile functions. Primary ciliary dyskinesia (PCD) is a type of ciliopathy caused by defects in motile cilia. T…
Integrin-Mediated Adhesion Promotes Centrosome Separation in Early Mitosis Open
Integrin-mediated adhesion to the extracellular matrix is a key regulator of the cell cycle, as demonstrated for the passage of the G1/S checkpoint and the completion of cytokinetic abscission. Here, integrin-dependent regulation of the ce…
View article: Structural equation modeling for factors influencing patients’ willingness to replace removable dentures
Structural equation modeling for factors influencing patients’ willingness to replace removable dentures Open
These findings provide a unified understanding of the shared decision-making process for denture replacement and highlight the pretreatment assessments that play a relevant role in patient treatment preferences.
Laparoscopic management of ruptured non communicating rudimentary horn in second trimester of pregnancy Open
Rudimentary horn is a developmental anomaly of the uterus. Pregnancy in rudimentary horn is rare occurrence with incidence of less than 1 in 150,000. Prerupture diagnosis of rudimentary horn pregnancy with USG is technically difficult, wit…