Denisa Bojková
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View article: The coronavirus envelope is conserved, contains bioactive lipids needed for replication, and is modulated in response to host inflammation
The coronavirus envelope is conserved, contains bioactive lipids needed for replication, and is modulated in response to host inflammation Open
Like all coronaviruses, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is surrounded by a lipid envelope hosting spike and other critical proteins. However, little is known about the biology of coronavirus membranes, their re…
View article: Papaverine Targets STAT Signaling: A Dual‐Action Therapy Option Against SARS‐CoV‐2
Papaverine Targets STAT Signaling: A Dual‐Action Therapy Option Against SARS‐CoV‐2 Open
Papaverine (PV) has been previously identified as a promising candidate in SARS‐CoV‐2 repurposing screens. In this study, we further investigated both its antiviral and immunomodulatory properties. PV displayed antiviral efficacy against S…
View article: Construction and Characterization of a High-Capacity Replication-Competent Murine Cytomegalovirus Vector for Gene Delivery
Construction and Characterization of a High-Capacity Replication-Competent Murine Cytomegalovirus Vector for Gene Delivery Open
We investigated the basic characteristics of a new murine cytomegalovirus (MCMV) vector platform. Using BAC technology, we engineered replication-competent recombinant MCMVs with deletions of up to 26% of the wild-type genome. To this end,…
View article: Correction: Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies
Correction: Biochemical characterization of protease activity of Nsp3 from SARS-CoV-2 and its inhibition by nanobodies Open
[This corrects the article DOI: 10.1371/journal.pone.0253364.].
View article: Trifluridine for treatment of mpox infection in drug combinations in ophthalmic cell models
Trifluridine for treatment of mpox infection in drug combinations in ophthalmic cell models Open
The Mpox virus can cause severe disease in the susceptible population with dermatologic and systemic manifestations. Furthermore, ophthalmic manifestations of mpox infection are well documented. Topical trifluridine (TFT) eye drops have be…
View article: Drug repurposing for the treatment of COVID-19: Targeting nafamostat to the lungs by a liposomal delivery system
Drug repurposing for the treatment of COVID-19: Targeting nafamostat to the lungs by a liposomal delivery system Open
Despite tremendous global efforts since the beginning of the COVID-19 pandemic, still only a limited number of prophylactic and therapeutic options are available. Although vaccination is the most effective measure in preventing morbidity a…
View article: Discovery of anti-SARS-CoV-2 secondary metabolites from the heartwood of Pterocarpus santalinus using multi-informative molecular networking
Discovery of anti-SARS-CoV-2 secondary metabolites from the heartwood of Pterocarpus santalinus using multi-informative molecular networking Open
A pigment-depleted extract from the heartwood of Pterocarpus santalinus L. f. (PS-DE) showed promising anti-SARS-CoV-2 activity with an IC 50 of 29.9 μg/mL in Caco-2-F03 cells. To determine the potential active constituents within the extr…
View article: SARS-CoV-2 Variants Show Different Host Cell Proteome Profiles With Delayed Immune Response Activation in Omicron-Infected Cells
SARS-CoV-2 Variants Show Different Host Cell Proteome Profiles With Delayed Immune Response Activation in Omicron-Infected Cells Open
The ancestral SARS-CoV-2 strain that initiated the Covid-19 pandemic at the end of 2019 has rapidly mutated into multiple variants of concern with variable pathogenicity and increasing immune escape strategies. However, differences in host…
View article: Identification of Z-Tyr-Ala-CHN2, a Cathepsin L Inhibitor with Broad-Spectrum Cell-Specific Activity against Coronaviruses, including SARS-CoV-2
Identification of Z-Tyr-Ala-CHN2, a Cathepsin L Inhibitor with Broad-Spectrum Cell-Specific Activity against Coronaviruses, including SARS-CoV-2 Open
The ongoing COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is partly under control by vaccination. However, highly potent and safe antiviral drugs for SARS-CoV-2 are still needed to avoid develop…
View article: Repurposing of the antibiotic nitroxoline for the treatment of mpox
Repurposing of the antibiotic nitroxoline for the treatment of mpox Open
The antiviral drugs tecovirimat, brincidofovir, and cidofovir are considered for mpox (monkeypox) treatment despite a lack of clinical evidence. Moreover, their use is affected by toxic side‐effects (brincidofovir, cidofovir), limited avai…
View article: Omicron‐induced interferon signaling prevents influenza A H1N1 and H5N1 virus infection
Omicron‐induced interferon signaling prevents influenza A H1N1 and H5N1 virus infection Open
Recent findings in permanent cell lines suggested that SARS‐CoV‐2 Omicron BA.1 induces a stronger interferon response than Delta. Here, we show that BA.1 and BA.5 but not Delta induce an antiviral state in air‐liquid interface cultures of …
View article: A Monoclonal Human Alveolar Epithelial Cell Line (“Arlo”) with Pronounced Barrier Function for Studying Drug Permeability and Viral Infections
A Monoclonal Human Alveolar Epithelial Cell Line (“Arlo”) with Pronounced Barrier Function for Studying Drug Permeability and Viral Infections Open
In the development of orally inhaled drug products preclinical animal models regularly fail to predict pharmacological as well as toxicological responses in humans. Models based on human cells and tissues are potential alternatives to anim…
View article: Identification of Natural Products Inhibiting SARS-CoV-2 by Targeting Viral Proteases: A Combined in Silico and in Vitro Approach
Identification of Natural Products Inhibiting SARS-CoV-2 by Targeting Viral Proteases: A Combined in Silico and in Vitro Approach Open
In this study, an integrated in silico-in vitro approach was employed to discover natural products (NPs) active against SARS-CoV-2. The two SARS-CoV-2 viral proteases, i.e., main protease (Mpro) and papain-like protease (PL…
View article: Identification of novel antiviral drug candidates using an optimized SARS-CoV-2 phenotypic screening platform
Identification of novel antiviral drug candidates using an optimized SARS-CoV-2 phenotypic screening platform Open
Reliable, easy-to-handle phenotypic screening platforms are needed for the identification of anti-SARS-CoV-2 compounds. Here, we present caspase 3/7 activity as a readout for monitoring the replication of SARS-CoV-2 isolates from different…
View article: Silver N-heterocyclic carbene complexes are potent uncompetitive inhibitors of the papain-like protease with antiviral activity against SARS-CoV-2
Silver N-heterocyclic carbene complexes are potent uncompetitive inhibitors of the papain-like protease with antiviral activity against SARS-CoV-2 Open
Silver NHC complexes are potent uncompetitive inhibitors of SARS-CoV-2 PL pro with distinct antiviral efficacy.
View article: Molecular Networking Unveils Anti-Sars-Cov-2 Constituents from Traditionally Used Remedies
Molecular Networking Unveils Anti-Sars-Cov-2 Constituents from Traditionally Used Remedies Open
View article: Repurposing of the antibiotic nitroxoline for the treatment of mpox
Repurposing of the antibiotic nitroxoline for the treatment of mpox Open
The antiviral drugs tecovirimat, brincidofovir, and cidofovir are considered for mpox (monkeypox) treatment despite a lack of clinical evidence. Moreover, their use is affected by toxic side-effects (brincidofovir, cidofovir), limited avai…
View article: Drug Sensitivity of Currently Circulating Mpox Viruses
Drug Sensitivity of Currently Circulating Mpox Viruses Open
suggest an increased incidence of ectopic pregnancy detected after abortion with the use of no-touch protocols. 5However, clinicians must maintain a high index of suspicion for ectopic pregnancy in persons presenting with suggestive sympto…
View article: Impact of the COVID‐19 pandemic on the circulation of other pathogens in England
Impact of the COVID‐19 pandemic on the circulation of other pathogens in England Open
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View article: Impact of the COVID-19 pandemic on the circulation of other pathogens in England
Impact of the COVID-19 pandemic on the circulation of other pathogens in England Open
The COVID-19 pandemic and the associated prevention measures did not only impact on the transmission of COVID-19 but also on the spread of other infectious diseases in an unprecedented natural experiment. Here, we analysed the transmission…
View article: Omicron-induced interferon signalling prevents influenza A virus infection
Omicron-induced interferon signalling prevents influenza A virus infection Open
Recent findings in permanent cell lines suggested that SARS-CoV-2 Omicron BA.1 induces a stronger interferon response than Delta. Here, we show that BA.1 and BA.5 but not Delta induce an antiviral state in air-liquid interface (ALI) cultur…
View article: USP22 controls type III interferon signaling and SARS-CoV-2 infection through activation of STING
USP22 controls type III interferon signaling and SARS-CoV-2 infection through activation of STING Open
View article: Synergism of interferon-beta with antiviral drugs against SARS-CoV-2 variants
Synergism of interferon-beta with antiviral drugs against SARS-CoV-2 variants Open
View article: Synergism of interferon-beta with antiviral drugs against SARS-CoV-2 variants
Synergism of interferon-beta with antiviral drugs against SARS-CoV-2 variants Open
Omicron BA.1 variant isolates were previously shown to replicate less effectively in interferon-competent cells and to be more sensitive to interferon treatment than a Delta isolate. Here, an Omicron BA.2 isolate displayed intermediate rep…
View article: Identification of novel antiviral drug candidates using an optimized SARS-CoV-2 phenotypic screening platform
Identification of novel antiviral drug candidates using an optimized SARS-CoV-2 phenotypic screening platform Open
Reliable, easy-to-handle phenotypic screening platforms are needed for the identification of anti-SARS-CoV-2 compounds. Here, we present caspase 3/7 activity as a read-out for monitoring the replication of SARS-CoV-2 isolates from differen…
View article: SARS-CoV-2 Omicron variant virus isolates are highly sensitive to interferon treatment
SARS-CoV-2 Omicron variant virus isolates are highly sensitive to interferon treatment Open
View article: SARS-CoV-2 screening strategies for returning international travellers: Evaluation of a rapid antigen test approach
SARS-CoV-2 screening strategies for returning international travellers: Evaluation of a rapid antigen test approach Open
RAT no earlier than the 5th day after arrival was a reliable method for detecting infectious travellers and can be recommended as an appropriate method for managing SARS-CoV-2 travel restrictions. Compliance to the regulations and a high s…
View article: Development and optimization of a high‐throughput screening assay for in vitro anti‐SARS‐CoV‐2 activity: Evaluation of 5676 Phase 1 Passed Structures
Development and optimization of a high‐throughput screening assay for in vitro anti‐SARS‐CoV‐2 activity: Evaluation of 5676 Phase 1 Passed Structures Open
Although vaccines are currently used to control the coronavirus disease 2019 (COVID‐19) pandemic, treatment options are urgently needed for those who cannot be vaccinated and for future outbreaks involving new severe acute respiratory synd…
View article: Development and optimisation of a high-throughput screening assay for in vitro anti–SARS-CoV-2 activity: evaluation of 5676 phase 1 passed structures
Development and optimisation of a high-throughput screening assay for in vitro anti–SARS-CoV-2 activity: evaluation of 5676 phase 1 passed structures Open
Although vaccines are currently used to control the coronavirus disease 2019 (COVID-19) pandemic, treatment options are urgently needed for those who cannot be vaccinated and for future outbreaks involving new severe acute respiratory synd…
View article: USP22 controls type III interferon signaling and SARS-CoV-2 infection through activation of STING
USP22 controls type III interferon signaling and SARS-CoV-2 infection through activation of STING Open
Pattern recognition receptors (PRRs) and interferons (IFNs) serve as essential antiviral defense against SARS-CoV-2, the causative agent of the COVID-19 pandemic. Type III IFN (IFN-λ) exhibit cell-type specific and long-lasting functions i…