Denish Piya
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View article: Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Based Isolation v2
Comparative Analysis of Phage Profiles in Environmental Samples: Metagenomic Sequencing Versus Culture-Based Isolation v2 Open
Culture-based isolation of bacteriophages from wastewater relies on a limited set of laboratory‐cultivable host strains, causing it to recover only a small fraction of the true viral diversity present in the influent (Chibani‐Chennoufi et …
View article: Comparative Analysis of Wastewater Influent Phage Profiles: Metagenomic Sequencing Versus Culture-Based Isolation v1
Comparative Analysis of Wastewater Influent Phage Profiles: Metagenomic Sequencing Versus Culture-Based Isolation v1 Open
Culture-based isolation of bacteriophages from wastewater relies on a limited set of laboratory‐cultivable host strains, causing it to recover only a small fraction of the true viral diversity present in the influent (Chibani‐Chennoufi et …
View article: Systematic and scalable genome-wide essentiality mapping to identify nonessential genes in phages
Systematic and scalable genome-wide essentiality mapping to identify nonessential genes in phages Open
Phages are one of the key ecological drivers of microbial community dynamics, function, and evolution. Despite their importance in bacterial ecology and evolutionary processes, phage genes are poorly characterized, hampering their usage in…
View article: Geochemical constraints on bacteriophage infectivity in terrestrial environments
Geochemical constraints on bacteriophage infectivity in terrestrial environments Open
Lytic phages can be potent and selective inhibitors of microbial growth and can have profound impacts on microbiome composition and function. However, there is uncertainty about the biogeochemical conditions under which phage predation mod…
View article: Genome-wide CRISPRi knockdown to map gene essentiality landscape in coliphages λ and P1
Genome-wide CRISPRi knockdown to map gene essentiality landscape in coliphages λ and P1 Open
Phages are one of the key ecological drivers of microbial community dynamics, function and evolution. Despite their importance in bacterial ecology and evolutionary processes, phage genes are poorly characterized, hampering their usage in …
View article: Geochemical constraints on bacteriophage infectivity in terrestrial environments
Geochemical constraints on bacteriophage infectivity in terrestrial environments Open
Lytic phages can be potent and selective inhibitors of microbial growth and can have profound impacts on microbiome composition and function. However, there is uncertainty about the biogeochemical conditions under which phage predation can…
View article: New Insights into the Structure and Assembly of Bacteriophage P1
New Insights into the Structure and Assembly of Bacteriophage P1 Open
Bacteriophage P1 is the premier transducing phage of E. coli. Despite its prominence in advancing E. coli genetics, modern molecular techniques have not been applied to thoroughly understand P1 structure. Here, we report the proteome of th…
View article: Complete Genome Sequence of Escherichia coli Bacteriophage U136B
Complete Genome Sequence of Escherichia coli Bacteriophage U136B Open
We report the genome sequence of bacteriophage U136B, which is reliant on the lipopolysaccharide and the antibiotic efflux protein TolC for infection of Escherichia coli and is a useful model for studying trade-offs and trade-ups that shap…
View article: High-throughput mapping of the phage resistance landscape in E. coli
High-throughput mapping of the phage resistance landscape in E. coli Open
Bacteriophages (phages) are critical players in the dynamics and function of microbial communities and drive processes as diverse as global biogeochemical cycles and human health. Phages tend to be predators finely tuned to attack specific…
View article: Genome-wide screens reveal Escherichia coli genes required for growth of T1-like phage LL5 and V5-like phage LL12
Genome-wide screens reveal Escherichia coli genes required for growth of T1-like phage LL5 and V5-like phage LL12 Open
The host factor requirements of phages and mechanisms of mutational phage insensitivity must be characterized for rational design of phage cocktails. To characterize host dependencies of two novel Escherichia coli phages, the T1-like sipho…
View article: High-throughput mapping of the phage resistance landscape in<i>E. coli</i>
High-throughput mapping of the phage resistance landscape in<i>E. coli</i> Open
Bacteriophages (phages) are critical players in the dynamics and function of microbial communities and drive processes as diverse as global biogeochemical cycles and human health. Phages tend to be predators finely tuned to attack specific…
View article: Complete Genome Sequence of Enterotoxigenic Escherichia coli Myophage LL12
Complete Genome Sequence of Enterotoxigenic Escherichia coli Myophage LL12 Open
This work describes the complete genome sequence of the virulent myophage LL12. The 136-kb LL12 genome is related to coliphage V5 and is a component in the prebiotic PreforPro. LL12 was isolated against enterotoxigenic Escherichia coli , w…
View article: Complete Genome Sequence of Enterotoxigenic Escherichia coli Siphophage LL5
Complete Genome Sequence of Enterotoxigenic Escherichia coli Siphophage LL5 Open
Here, we describe the complete genome sequence of siphophage LL5. LL5 is a T1-like phage isolated against enterotoxigenic Escherichia coli , which causes traveler’s diarrhea. LL5 is included as a component phage in the commercial prebiotic…
View article: Genome-wide Screens Reveal Escherichia Coli Genes Required for Growth of T1-like Phage LL5 and rV5-like Phage LL12
Genome-wide Screens Reveal Escherichia Coli Genes Required for Growth of T1-like Phage LL5 and rV5-like Phage LL12 Open
Factors affecting the host-virus interaction must be understood for the effective application of bacteriophages to combat bacterial pathogens. Two novel E. coli phages, the T1-like siphophage LL5 and the rV5-like myophage LL12, were subjec…
View article: Interactions between Host and Phage Encoded Factors Shape Phage Infection
Interactions between Host and Phage Encoded Factors Shape Phage Infection Open
Evolution of phages and their bacterial hosts are directed by interaction between phage and host-encoded factors. These interactions have resulted in the development of several defense and counter-defense strategies such as DNA restriction…
View article: The multicomponent antirestriction system of phage P1 is linked to capsid morphogenesis
The multicomponent antirestriction system of phage P1 is linked to capsid morphogenesis Open
Summary Bacterial Type I restriction‐modification (R‐M) systems present a major barrier to foreign DNA entering the bacterial cell. The temperate phage P1 packages several proteins into the virion that protect the phage DNA from host restr…