Derek Fleming
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View article: Safety trial assessing 1.77 cm2 H2O2 producing electrochemical bandages on healthy human skin
Safety trial assessing 1.77 cm2 H2O2 producing electrochemical bandages on healthy human skin Open
Mammalian skin maintains complex repair mechanisms after barrier disruption. Chronic wounds develop when these events are not efficiently completed, increasing infection risk. With ~2% of the population afflicted yearly, chronic wound care…
View article: Electrochemical Catheter Hub Operated by a Wearable Micropotentiostat Prevents <i>Acinetobacter baumannii</i> Infection In Vitro
Electrochemical Catheter Hub Operated by a Wearable Micropotentiostat Prevents <i>Acinetobacter baumannii</i> Infection In Vitro Open
Intraluminal infection of central venous catheters, used for long‐term treatment, can result in central line‐associated bloodstream infection (CLABSI). These infections can be challenging to prevent and treat due to formation of biofilms w…
View article: Comparative efficacy of levofloxacin, azithromycin, and doxycycline prophylaxis and treatment in an experimental <i>Ureaplasma</i> murine lung infection model
Comparative efficacy of levofloxacin, azithromycin, and doxycycline prophylaxis and treatment in an experimental <i>Ureaplasma</i> murine lung infection model Open
Although lung transplant recipients who develop Ureaplasma- associated hyperammonemia syndrome are treated with Ureaplasma -targeted antibiotics, optimal therapy is incompletely defined, and typically not based on real-time antimicrobial s…
View article: P-1091. Hydrogen peroxide generating electrochemical bandage for the treatment of wound biofilm infections - preliminary demonstration of device safety on healthy human skin
P-1091. Hydrogen peroxide generating electrochemical bandage for the treatment of wound biofilm infections - preliminary demonstration of device safety on healthy human skin Open
Background Wound biofilm infections impact ∼2% of the United States and at-risk individuals often have underlying morbidities such as diabetes. Antibiotics and topical approaches like debridement or silver dressings have had limited succes…
View article: P-647. Comparative efficacy of antibiotic regimens against <i>Ureaplasma</i> species with varying antibiotic resistance profiles in a mouse model of lung infection
P-647. Comparative efficacy of antibiotic regimens against <i>Ureaplasma</i> species with varying antibiotic resistance profiles in a mouse model of lung infection Open
Background Lung transplant recipients (LTRs) can experience hyperammonemia syndrome (HS) correlated with lung infection by ammonia-producing Ureaplasma species. HS in LTRs can be managed by Ureaplasma-directed antibiotic therapy; however, …
View article: P-66. Comparison of Periprosthetic Tissue and Cement Spacer Sonicate Fluid Cultures at the Second Stage of Two-Stage Hip and Knee Revision Arthroplasty Surgery for Periprosthetic Joint Infection
P-66. Comparison of Periprosthetic Tissue and Cement Spacer Sonicate Fluid Cultures at the Second Stage of Two-Stage Hip and Knee Revision Arthroplasty Surgery for Periprosthetic Joint Infection Open
Background Chronic periprosthetic joint infection is commonly managed with two-stage exchange. Clinically, evaluation for persistent infection at the second stage of exchange may inform management. The aim of this study was to evaluate the…
View article: Prior immunity to <i>Ureaplasma urealyticum</i> protects against respiratory infection in immunosuppressed mice
Prior immunity to <i>Ureaplasma urealyticum</i> protects against respiratory infection in immunosuppressed mice Open
Ureaplasma species can cause systemic infections in immunocompromised hosts, including lung transplant recipients. Here, we investigated the impact of prior exposure to Ureaplasma urealyticum on the risk of U. urealyticum and Ureaplasma pa…
View article: Electrochemical HOCl Production Modeling for an Electrochemical Catheter
Electrochemical HOCl Production Modeling for an Electrochemical Catheter Open
Hypochlorous acid (HOCl) is a strong oxidizing agent that damages cells by interacting with lipids, nucleic acids, sulfur-containing amino acids, and membrane components. It is an endogenous substance produced by the immune system to prote…
View article: HOCl-producing electrochemical bandage is active in murine polymicrobial wound infection
HOCl-producing electrochemical bandage is active in murine polymicrobial wound infection Open
Wound infections, exacerbated by the prevalence of antibiotic-resistant bacterial pathogens, necessitate innovative antimicrobial approaches. Polymicrobial infections, often involving Pseudomonas aeruginosa and methicillin-resistant Staphy…
View article: Evaluation of treatment of methicillin-resistant <i>Staphylococcus aureus</i> biofilms with intermittent electrochemically generated H <sub>2</sub> O <sub>2</sub> or HOCl
Evaluation of treatment of methicillin-resistant <i>Staphylococcus aureus</i> biofilms with intermittent electrochemically generated H <sub>2</sub> O <sub>2</sub> or HOCl Open
Chronic wound infections can be difficult to treat and may lead to impaired healing and worsened patient outcomes. Novel treatment strategies are needed. This study evaluated the effects of intermittently produced hydrogen peroxide (H 2 O …
View article: Evaluation of Treatment of Methicillin-Resistant<i>Staphylococcus aureus</i>Biofilms with Intermittent Electrochemically-Generated H<sub>2</sub>O<sub>2</sub>or HOCl
Evaluation of Treatment of Methicillin-Resistant<i>Staphylococcus aureus</i>Biofilms with Intermittent Electrochemically-Generated H<sub>2</sub>O<sub>2</sub>or HOCl Open
Chronic wound infections can be difficult to treat and may lead to impaired healing and worsened patient outcomes. Novel treatment strategies are needed. This study evaluated effects of intermittently produced H 2 O 2 and HOCl, generated v…
View article: DUAL ACTION ELECTROCHEMICAL BANDAGE OPERATED by a PROGRAMMABLE MULTIMODAL WEARABLE POTENTIOSTAT
DUAL ACTION ELECTROCHEMICAL BANDAGE OPERATED by a PROGRAMMABLE MULTIMODAL WEARABLE POTENTIOSTAT Open
Electrochemical bandages (e-bandages) can be applied to biofilm-infected wounds to generate reactive oxygen species, such as hypochlorous acid (HOCl) or hydrogen peroxide (H 2 O 2 ). The e-bandage-generated HOCl or H 2 O 2 kills biofilms i…
View article: HOCl-producing Electrochemical Bandage is Active in Murine Polymicrobial Wound Infection
HOCl-producing Electrochemical Bandage is Active in Murine Polymicrobial Wound Infection Open
Wound infections, exacerbated by the prevalence of antibiotic-resistant bacterial pathogens, necessitate innovative antimicrobial approaches. Polymicrobial infections, often involving Pseudomonas aeruginosa and methicillin-resistant Staphy…
View article: HOCl-producing electrochemical bandage for treating <i>Pseudomonas aeruginosa</i> -infected murine wounds
HOCl-producing electrochemical bandage for treating <i>Pseudomonas aeruginosa</i> -infected murine wounds Open
The growing threat of antibiotic-resistant bacterial pathogens necessitates the development of alternative antimicrobial approaches. This is particularly true for chronic wound infections, which commonly harbor biofilm-dwelling bacteria. A…
View article: 2120. Activity of a Hypochlorous Acid-Generating Electrochemical Bandage for Treating Experimental Murine Wounds Infected with <i>Pseudomonas aeruginosa</i>
2120. Activity of a Hypochlorous Acid-Generating Electrochemical Bandage for Treating Experimental Murine Wounds Infected with <i>Pseudomonas aeruginosa</i> Open
Background Biofilms formed by antibiotic-resistant bacteria in wound beds present unique challenges in terms of treating wound infections. In this work, the in vivo anti-biofilm activity of a novel electrochemical bandage (e-bandage) desig…
View article: The biofilm community resurfaces: new findings and post-pandemic progress
The biofilm community resurfaces: new findings and post-pandemic progress Open
The ninth American Society for Microbiology Conference on Biofilms was convened in-person on 13–17 November 2022 in Charlotte, NC. As the first of these conferences since prior to the start of the COVID-19 pandemic, the energy among the pa…
View article: HOCl-producing Electrochemical Bandages for Treating<i>Pseudomonas aeruginosa</i>-Infected Murine Wounds
HOCl-producing Electrochemical Bandages for Treating<i>Pseudomonas aeruginosa</i>-Infected Murine Wounds Open
A novel electrochemical bandage (e-bandage) delivering low-level hypochlorous acid (HOCl) was evaluated against Pseudomonas aeruginosa murine wound biofilms. 5 mm skin wounds were created on the dorsum of Swiss-Webster mice and infected wi…
View article: In Vivo Activity of Hydrogen‐Peroxide Generating Electrochemical Bandage Against Murine Wound Infections
In Vivo Activity of Hydrogen‐Peroxide Generating Electrochemical Bandage Against Murine Wound Infections Open
Biofilms formed by antibiotic‐resistant bacteria in wound beds challenge the treatment of wound infections. In this work, the activity of a novel electrochemical bandage (e‐bandage) composed of carbon fabric and controlled by a wearable po…
View article: Flurofamide Prevention and Treatment of <i>Ureaplasma</i> -Induced Hyperammonemia
Flurofamide Prevention and Treatment of <i>Ureaplasma</i> -Induced Hyperammonemia Open
Ureaplasma -associated hyperammonemia syndrome occurs in 4% of lung transplant recipients and has historically been almost universally fatal. While Ureaplasma -targeted antibiotics have been shown to be protective, the possibility of under…
View article: Contribution of Pseudomonas aeruginosa Exopolysaccharides Pel and Psl to Wound Infections
Contribution of Pseudomonas aeruginosa Exopolysaccharides Pel and Psl to Wound Infections Open
Biofilms are the cause of most chronic bacterial infections. Living within the biofilm matrix, which is made of extracellular substances, including polysaccharides, proteins, eDNA, lipids and other molecules, provides microorganisms protec…
View article: Contribution of Uremia to <i>Ureaplasma</i> -Induced Hyperammonemia
Contribution of Uremia to <i>Ureaplasma</i> -Induced Hyperammonemia Open
Ureaplasma -induced hyperammonemia is a deadly complication affecting around 4% of lung transplant recipients and, to a lesser extent, other solid organ transplant patients. Understanding the underlying mechanisms will inform patient manag…
View article: Contribution of Uremia to <i>Ureaplasma</i>-Induced Hyperammonemia
Contribution of Uremia to <i>Ureaplasma</i>-Induced Hyperammonemia Open
Lung transplant recipients (LTRs) are vulnerable to unexplained hyperammonemia syndrome (HS) in the early post-operative period, a condition typically unresponsive to non-antibiotic interventions. Recently, we showed that HS in LTRs is str…
View article: Detection of bacterial fluorescence from in vivo wound biofilms using a point‐of‐care fluorescence imaging device
Detection of bacterial fluorescence from in vivo wound biofilms using a point‐of‐care fluorescence imaging device Open
Wound biofilms must be identified to target disruption and bacterial eradication but are challenging to detect with standard clinical assessment. This study tested whether bacterial fluorescence imaging could detect porphyrin‐producing bac…
View article: Effect of collagen and EPS components on the viscoelasticity of <i>Pseudomonas aeruginosa</i> biofilms
Effect of collagen and EPS components on the viscoelasticity of <i>Pseudomonas aeruginosa</i> biofilms Open
Pseudomonas aeruginosa is an opportunistic pathogen that causes thousands of deaths every year in part due to its ability to form biofilms composed of bacteria embedded in a matrix of self-secreted extracellular polysaccharides (EPS), e-DN…
View article: The evolution of virulence in <i>Pseudomonas aeruginosa</i> during chronic wound infection
The evolution of virulence in <i>Pseudomonas aeruginosa</i> during chronic wound infection Open
Opportunistic pathogens are associated with a number of chronic human infections, yet the evolution of virulence in these organisms during chronic infection remains poorly understood. Here, we tested the evolution of virulence in the human…