Derrick E. Rancourt
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View article: Generation of two human induced pluripotent stem cell lines from patient-derived fibroblasts with severe congenital defects of the vertebrae and neural tube
Generation of two human induced pluripotent stem cell lines from patient-derived fibroblasts with severe congenital defects of the vertebrae and neural tube Open
Severe congenital defects of the vertebrae and neural tube arise from complex genetic, environmental, and nutritional factors during early pregnancy. Despite extensive research, the root causes of these conditions remain poorly understood.…
View article: Mesenchymal progenitor–derived proteoglycan 4 regulates the transdifferentiation of chondrocytes into osteoblasts during fracture healing
Mesenchymal progenitor–derived proteoglycan 4 regulates the transdifferentiation of chondrocytes into osteoblasts during fracture healing Open
Introduction Proteoglycan 4 (PRG4), also known as lubricin, is essential for maintaining tissue homeostasis and acts as a lubricant that protects joint surfaces from wear and tear. Our previous studies have demonstrated that PRG4 plays mul…
View article: Human transferrin and lactoferrin cooperatively support<i>Neisseria meningitidis</i>colonization in the murine nasopharynx
Human transferrin and lactoferrin cooperatively support<i>Neisseria meningitidis</i>colonization in the murine nasopharynx Open
Neisseria meningitidis is a human-restricted bacteria that is a normal nasopharyngeal resident, yet it can also disseminate, causing invasive meningococcal disease. Meningococci are highly adapted to life in humans, with human-specific vir…
View article: Robust bioprocess design and evaluation of commercial media for the serial expansion of human induced pluripotent stem cell aggregate cultures in vertical-wheel bioreactors
Robust bioprocess design and evaluation of commercial media for the serial expansion of human induced pluripotent stem cell aggregate cultures in vertical-wheel bioreactors Open
Background While pluripotent stem cell (PSC) therapies move toward clinical and commercial applications at a rapid rate, manufacturing reproducibility and robustness are notable bottlenecks in regulatory approval. Therapeutic applications …
View article: A retinoid analogue, TTNPB, promotes clonal expansion of human pluripotent stem cells by upregulating CLDN2 and HoxA1
A retinoid analogue, TTNPB, promotes clonal expansion of human pluripotent stem cells by upregulating CLDN2 and HoxA1 Open
Enzymatic dissociation of human pluripotent stem cells (hPSCs) into single cells during routine passage leads to massive cell death. Although the Rho-associated protein kinase inhibitor, Y-27632 can enhance hPSC survival and proliferation …
View article: Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing
Efficient derivation of transgene-free porcine induced pluripotent stem cells enables in vitro modeling of species-specific developmental timing Open
Sus scrofa domesticus (pig) has served as a superb large mammalian model for biomedical studies because of its comparable physiology and organ size to humans. The derivation of transgene-free porcine induced pluripotent stem cells (PiPSCs)…
View article: Robust Bioprocess Design and Evaluation of Commercial Media for the Serial Expansion of Human Induced Pluripotent Stem Cell Aggregate Cultures in Vertical-Wheel Bioreactors
Robust Bioprocess Design and Evaluation of Commercial Media for the Serial Expansion of Human Induced Pluripotent Stem Cell Aggregate Cultures in Vertical-Wheel Bioreactors Open
Background: While pluripotent stem cell (PSC) therapies move toward clinical and commercial applications at a rapid rate, manufacturing reproducibility and robustness are notable bottlenecks in regulatory approval. Therapeutic applications…
View article: Modulation of Reoviral Cytolysis (II): Cellular Stemness
Modulation of Reoviral Cytolysis (II): Cellular Stemness Open
Oncolytic viruses (OVs) are an emerging cancer therapeutic that are intended to act by selectively targeting and lysing cancerous cells and by stimulating anti-tumour immune responses, while leaving normal cells mainly unaffected. Reovirus…
View article: Exogenously delivered iPSCs disrupt the natural repair response of endogenous MPCs after bone injury
Exogenously delivered iPSCs disrupt the natural repair response of endogenous MPCs after bone injury Open
Promoting bone healing including fracture non-unions are promising targets for bone tissue engineering due to the limited success of current clinical treatment methods. There has been significant research on the use of stem cells with and …
View article: Erratum to “Comparison of human skin‐ and nerve‐derived Schwann cells reveals many similarities and subtle genomic and functional differences”
Erratum to “Comparison of human skin‐ and nerve‐derived Schwann cells reveals many similarities and subtle genomic and functional differences” Open
View article: The Proliferation of Pre-Pubertal Porcine Spermatogonia in Stirred Suspension Bioreactors Is Partially Mediated by the Wnt/β-Catenin Pathway
The Proliferation of Pre-Pubertal Porcine Spermatogonia in Stirred Suspension Bioreactors Is Partially Mediated by the Wnt/β-Catenin Pathway Open
Male survivors of childhood cancer are at risk of suffering from infertility in adulthood because of gonadotoxic chemotherapies. For adult men, sperm collection and preservation are routine procedures prior to treatment; however, this is n…
View article: Author response for "Computational fluid dynamic characterization of vertical‐wheel bioreactors used for effective scale‐up of human induced pluripotent stem cell aggregate culture"
Author response for "Computational fluid dynamic characterization of vertical‐wheel bioreactors used for effective scale‐up of human induced pluripotent stem cell aggregate culture" Open
View article: Enhanced Osteogenic Differentiation of Pluripotent Stem Cells via γ-Secretase Inhibition
Enhanced Osteogenic Differentiation of Pluripotent Stem Cells via γ-Secretase Inhibition Open
Bone healing is a complex, well-organized process. Multiple factors regulate this process, including growth factors, hormones, cytokines, mechanical stimulation, and aging. One of the most important signaling pathways that affect bone heal…
View article: Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors
Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors Open
View article: Overcoming Bioprocess Bottlenecks in the Large-Scale Expansion of High Quality hiPSC Aggregates in Vertical-Wheel Stirred Suspension Bioreactors
Overcoming Bioprocess Bottlenecks in the Large-Scale Expansion of High Quality hiPSC Aggregates in Vertical-Wheel Stirred Suspension Bioreactors Open
Background Human induced pluripotent stem cells (hiPSCs) hold enormous promise in accelerating breakthroughs in understanding human development, drug screening, disease modeling and cell and gene therapies. Their potential, however, has be…
View article: Cell-Based Therapy Manufacturing in Stirred Suspension Bioreactor: Thoughts for cGMP Compliance
Cell-Based Therapy Manufacturing in Stirred Suspension Bioreactor: Thoughts for cGMP Compliance Open
Cell-based therapy (CBT) is attracting much attention to treat incurable diseases. In recent years, several clinical trials have been conducted using human pluripotent stem cells (hPSCs), and other potential therapeutic cells. Various priv…
View article: Transgenic Expression of <i>Cacna1f</i> Rescues Vision and Retinal Morphology in a Mouse Model of Congenital Stationary Night Blindness 2A (CSNB2A)
Transgenic Expression of <i>Cacna1f</i> Rescues Vision and Retinal Morphology in a Mouse Model of Congenital Stationary Night Blindness 2A (CSNB2A) Open
These data have relevance in the treatment of CSNB2A and in understanding how photoreceptor integration might be achieved in retinas in which photoreceptors have been lost, such as retinitis pigmentosa, age-related macular degeneration, an…
View article: Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells
Stirred suspension bioreactors maintain naïve pluripotency of human pluripotent stem cells Open
View article: Overcoming Bioprocess Bottlenecks in the Large-Scale Expansion of High Quality hiPSC Aggregates in Vertical-Wheel Stirred Suspension Bioreactors
Overcoming Bioprocess Bottlenecks in the Large-Scale Expansion of High Quality hiPSC Aggregates in Vertical-Wheel Stirred Suspension Bioreactors Open
BackgroundHuman induced pluripotent stem cells (hiPSCs) hold enormous promise in accelerating breakthroughs in understanding human development, drug screening, disease modeling and cell and gene therapies. Their potential, however, has bee…
View article: Optimized serial expansion of human induced pluripotent stem cells using low-density inoculation to generate clinically relevant quantities in vertical-wheel bioreactors
Optimized serial expansion of human induced pluripotent stem cells using low-density inoculation to generate clinically relevant quantities in vertical-wheel bioreactors Open
Human induced pluripotent stem cells (hiPSCs) have generated a great deal of attention owing to their capacity for self-renewal and differentiation into the three germ layers of the body. Their discovery has facilitated a new era in biomed…
View article: Vicarious Learning through Informational Interviews: A New Experiential Learning Tool
Vicarious Learning through Informational Interviews: A New Experiential Learning Tool Open
Many students find it difficult to transition into the workforce successfully. They often accept unsuitable positions through happenstance rather than actively planning their career trajectories. This is due to their unawareness of career …
View article: Cytokine Directed Chondroblast Trans-Differentiation: JAK Inhibition Facilitates Direct Reprogramming of Fibroblasts to Chondroblasts
Cytokine Directed Chondroblast Trans-Differentiation: JAK Inhibition Facilitates Direct Reprogramming of Fibroblasts to Chondroblasts Open
Osteoarthritis (OA) is a degenerative disease of the hyaline articular cartilage. This disease is progressive and may lead to disability. Researchers proposed many regenerative approaches to treat osteoarthritis, including stem cells. Tran…
View article: Post-Passage rock inhibition induces cytoskeletal aberrations and apoptosis in Human embryonic stem cells
Post-Passage rock inhibition induces cytoskeletal aberrations and apoptosis in Human embryonic stem cells Open
Human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) are prone to anoikis after single cell dissociation. The small molecule, Y-27632 is known to increase survival of hESCs and hiPSCs by inhibiting the Rho-associa…
View article: Reversible Mitochondrial Fragmentation in iPSC-Derived Cardiomyocytes from Children with DCMA, a Mitochondrial Cardiomyopathy
Reversible Mitochondrial Fragmentation in iPSC-Derived Cardiomyocytes from Children with DCMA, a Mitochondrial Cardiomyopathy Open
Background The dilated cardiomyopathy with ataxia syndrome (DCMA) is an understudied autosomal recessive disease caused by loss-of-function mutations in the poorly characterized gene DNAJC19 . Clinically, DCMA is commonly associated with h…
View article: Integrated Biologics Manufacturing in Stirred-Suspension Bioreactor: A Stem Cell Perspective
Integrated Biologics Manufacturing in Stirred-Suspension Bioreactor: A Stem Cell Perspective Open
Stem cell therapy is garnering attention as several clinical trials have taken place in the recent years by using human pluripotent stem cells (hPSCs). Hundreds of biotechnological companies are investing to find a permanent cure for diffi…
View article: Concise Review: Molecular Cytogenetics and Quality Control: Clinical Guardians for Pluripotent Stem Cells
Concise Review: Molecular Cytogenetics and Quality Control: Clinical Guardians for Pluripotent Stem Cells Open
Now that induced pluripotent stem cell (iPSC)-based transplants have been performed in humans and organizations have begun producing clinical-grade iPSCs, it is imperative that strict quality control standards are agreed upon. This is esse…
View article: Going (Reo)Viral: Factors Promoting Successful Reoviral Oncolytic Infection
Going (Reo)Viral: Factors Promoting Successful Reoviral Oncolytic Infection Open
Oncolytic viruses show intriguing potential as cancer therapeutic agents. These viruses are capable of selectively targeting and killing cancerous cells while leaving healthy cells largely unaffected. The use of oncolytic viruses for cance…
View article: An Integrated Approach toward the Biomanufacturing of Engineered Cell Therapy Products in a Stirred-Suspension Bioreactor
An Integrated Approach toward the Biomanufacturing of Engineered Cell Therapy Products in a Stirred-Suspension Bioreactor Open
View article: 344. En Route to Non-Viral Genetic Engineering: Kinetics of DNA Uptake and Transgene Expression Following Repeated Transfection with Multiple Episomal Plasmids in Human Primary Fibroblast
344. En Route to Non-Viral Genetic Engineering: Kinetics of DNA Uptake and Transgene Expression Following Repeated Transfection with Multiple Episomal Plasmids in Human Primary Fibroblast Open
Non-viral approach to cellular reprogramming or genome editing of mammalian cells often involve co-delivery of multiple types of nucleic acid molecules. Whether the method involves co-transfection with multiple episomal plasmid DNAs, a mix…
View article: 343. Non-Viral Transfection of Primary Human Fibroblasts in Suspension Microcarrier Culture
343. Non-Viral Transfection of Primary Human Fibroblasts in Suspension Microcarrier Culture Open
Suspension bioreactors have been employed for the large-scale production of recombinant biomacromolecules and expansion of primary tissue-derived cells for clinical applications. However, the bioprocess requires establishing a stably-expre…