David F. Callen
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View article: Supplementary Figure 1 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 1 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 43K, BEZ235 inhibits phosphorylation of PI3K/mTOR. Western blot showing phospho-AKT (Ser473) and phospho-S6 (Ser235/236) expression in serum-starved H460 and A549 cells treated with indicated doses of BEZ235 and stimulated with …
View article: Supplementary Figure 4 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Supplementary Figure 4 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
GSK-LSD1, Doxorubicin and AraC have no effect on KDM1A and KDM1B expression
View article: Supplementary Figure Legends 1-8 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure Legends 1-8 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 85K
View article: Supplementary Figure 8 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 8 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 4.8MB, Representative immunofluorescence images of phospho-DNA-PKcs (Thr2609) foci in H460 cells at 30 minutes after 10 Gy radiation (x 60 magnification). Green, phospho-DNA-PKcs foci. Blue, DAPI staining.
View article: Data from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Data from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Multi-agent chemotherapeutic regimes remain the cornerstone treatment for Ewing sarcoma, the second most common bone malignancy diagnosed in pediatric and young adolescent populations. We have reached a therapeutic ceiling with conventiona…
View article: Data from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Data from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
DNA-dependent protein kinase (DNA-PK) plays a pivotal role in the repair of DNA double-strand breaks (DSB) and is centrally involved in regulating cellular radiosensitivity. Here, we identify DNA-PK as a key therapeutic target for augmenti…
View article: Supplementary Figure 1 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 1 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 43K, BEZ235 inhibits phosphorylation of PI3K/mTOR. Western blot showing phospho-AKT (Ser473) and phospho-S6 (Ser235/236) expression in serum-starved H460 and A549 cells treated with indicated doses of BEZ235 and stimulated with …
View article: Supplementary Figure 2 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Supplementary Figure 2 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Targeted KDM1A inhibition and EWS/FLI knockdown induces similar transcriptional signatures
View article: Supplementary Figure 6 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 6 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 4.6MB, Representative immunofluorescence images of phospho-ATM (Ser1981) foci in H460 and A549 cells at 30 minutes after 10 Gy dose of radiation (x 60 magnification). Green, phospho-ATM foci. Blue, DAPI staining.
View article: Supplementary Figure 7 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 7 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 171K, Induction of accelerated senescence by BEZ235 in irradiated cells is p53-dependent. A, Representative images of SA-�Gal activity cells at 48 hours after 10 Gy radiation (x 200 magnification). B, Western blot showing total …
View article: Supplementary Figure Legends 1-8 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure Legends 1-8 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 85K
View article: Supplementary Figure 6 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 6 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 4.6MB, Representative immunofluorescence images of phospho-ATM (Ser1981) foci in H460 and A549 cells at 30 minutes after 10 Gy dose of radiation (x 60 magnification). Green, phospho-ATM foci. Blue, DAPI staining.
View article: Data from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Data from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Multi-agent chemotherapeutic regimes remain the cornerstone treatment for Ewing sarcoma, the second most common bone malignancy diagnosed in pediatric and young adolescent populations. We have reached a therapeutic ceiling with conventiona…
View article: Supplementary Figure 1 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Supplementary Figure 1 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Validation of KDM1A knockdown in Ewing sarcoma cells
View article: Supplementary Table S1 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Supplementary Table S1 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Cell line source/culture conditions, primer/shRNA sequences, antibodies utilized in this study and additional methods
View article: Data from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Data from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
DNA-dependent protein kinase (DNA-PK) plays a pivotal role in the repair of DNA double-strand breaks (DSB) and is centrally involved in regulating cellular radiosensitivity. Here, we identify DNA-PK as a key therapeutic target for augmenti…
View article: Supplementary Figure 3 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Supplementary Figure 3 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Cytotoxic effects of the irreversible KDM1A inhibitors GSK-LSD1 and Tranylcypromine
View article: Supplementary Figure 4 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 4 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 2MB, IL-8, MIF and PAI-1 levels in supernatant from H460 and A549 cells after 96 hours treatment. Supernatant volumes were normalized to cell number and analyzed using cytokine antibody arrays.
View article: Supplementary Figure 7 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 7 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 171K, Induction of accelerated senescence by BEZ235 in irradiated cells is p53-dependent. A, Representative images of SA-�Gal activity cells at 48 hours after 10 Gy radiation (x 200 magnification). B, Western blot showing total …
View article: Supplementary Figure 8 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 8 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 4.8MB, Representative immunofluorescence images of phospho-DNA-PKcs (Thr2609) foci in H460 cells at 30 minutes after 10 Gy radiation (x 60 magnification). Green, phospho-DNA-PKcs foci. Blue, DAPI staining.
View article: Supplementary Figure 3 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Supplementary Figure 3 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Cytotoxic effects of the irreversible KDM1A inhibitors GSK-LSD1 and Tranylcypromine
View article: Supplementary Table S2 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Supplementary Table S2 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Summary of Ewing sarcoma KDM1A expression studies and genes consistently induced/repressed following SP-2509 treatment
View article: Supplementary Figure 4 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 4 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 2MB, IL-8, MIF and PAI-1 levels in supernatant from H460 and A549 cells after 96 hours treatment. Supernatant volumes were normalized to cell number and analyzed using cytokine antibody arrays.
View article: Supplementary Figure 2 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 2 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 7.4MB, TUNEL assay in H460 and A549 cells after 48 hours treatment. H460 cells irradiated with 20 Gy were used as a positive control.
View article: Supplementary Figure 6 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Supplementary Figure 6 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Protein expression of ER-stress response genes following treatment with SP-2509
View article: Supplementary Table S1 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Supplementary Table S1 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Cell line source/culture conditions, primer/shRNA sequences, antibodies utilized in this study and additional methods
View article: Supplementary Table S2 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Supplementary Table S2 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Summary of Ewing sarcoma KDM1A expression studies and genes consistently induced/repressed following SP-2509 treatment
View article: Supplementary Figure 3 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 3 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 1.8MB, BEZ235 does not induce abnormal mitoses in irradiated H460 and A549 cells. Number of abnormal mitoses at 48 hours following indicated treatment. An average of 70 cells per group was counted to determine mean +/- SEM.
View article: Supplementary Figure 5 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells
Supplementary Figure 5 from Inhibition of DNA-Dependent Protein Kinase Induces Accelerated Senescence in Irradiated Human Cancer Cells Open
PDF file - 9MB, Representative immunofluorescence images of gamma H2AX (Ser139) foci in H460 and A549 cells at indicated time points after 4 Gy radiation (x 60 magnification). Green, �H2AX foci. Blue, DAPI staining. Below, Number of gamma …
View article: Supplementary Figure 5 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response
Supplementary Figure 5 from Therapeutic Targeting of KDM1A/LSD1 in Ewing Sarcoma with SP-2509 Engages the Endoplasmic Reticulum Stress Response Open
Hypersensitive SP-2509 cell lines display similar transcriptomic profiles