Dhani Ram Mahato
YOU?
Author Swipe
View article: Single-Molecule Kinetic Exploration of Functional Substates in an Evolving Phosphotriesterase
Single-Molecule Kinetic Exploration of Functional Substates in an Evolving Phosphotriesterase Open
Enzymes achieve catalysis by dynamically sampling diverse conformational states. Beyond this plasticity, individual enzyme molecules occupy metastable substates, forming an ensemble of functional substates within a population. Since shifts…
View article: Evolution Enhances Kemp Eliminase Activity by Optimizing Oxyanion Stabilization and Conformational Flexibility
Evolution Enhances Kemp Eliminase Activity by Optimizing Oxyanion Stabilization and Conformational Flexibility Open
The base‐promoted Kemp elimination reaction has been used as a model system for enzyme design. Among the multiple computationally designed and evolved Kemp eliminases generated along the years, the HG3‐to‐HG3.17 evolutionary trajectory is …
View article: Retracing the Rapid Evolution of an Herbicide-Degrading Enzyme by Protein Engineering
Retracing the Rapid Evolution of an Herbicide-Degrading Enzyme by Protein Engineering Open
The mechanisms underlying the rapid evolution of novel enzymatic activities from promiscuous side activities are poorly understood. Recently emerged enzymes catalyzing the catabolic degradation of xenobiotic substances that have been sprea…
View article: The two-domain elevator-type mechanism of zinc-transporting ZIP proteins
The two-domain elevator-type mechanism of zinc-transporting ZIP proteins Open
Zinc is essential for all organisms and yet detrimental at elevated levels. Hence, homeostasis of this metal is tightly regulated. The Zrt/Irt-like proteins (ZIPs) represent the only zinc importers in metazoans. Mutations in human ZIPs cau…
View article: <scp>PcoB</scp> is a defense outer membrane protein that facilitates cellular uptake of copper
<span>PcoB</span> is a defense outer membrane protein that facilitates cellular uptake of copper Open
Copper (Cu) is one of the most abundant trace metals in all organisms, involved in a plethora of cellular processes. Yet elevated concentrations of the element are harmful, and interestingly prokaryotes are more sensitive for environmental…
View article: PcoB is a defense outer membrane protein that facilitates cellular uptake of copper
PcoB is a defense outer membrane protein that facilitates cellular uptake of copper Open
Copper (Cu) is one of the most abundant trace metals in all organisms, involved in a plethora of cellular processes. Yet elevated concentrations of the element are harmful, and interestingly prokaryotes are more sensitive for environmental…
View article: Author response: Structure and ion-release mechanism of PIB-4-type ATPases
Author response: Structure and ion-release mechanism of PIB-4-type ATPases Open
Article Figures and data Abstract Editor's evaluation eLife digest Introduction Results and discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Trans…
View article: A missense mutation converts the Na+,K+-ATPase into an ion channel and causes therapy-resistant epilepsy
A missense mutation converts the Na+,K+-ATPase into an ion channel and causes therapy-resistant epilepsy Open
The ion pump Na+,K+-ATPase is a critical determinant of neuronal excitability; however, its role in the etiology of diseases of the central nervous system (CNS) is largely unknown. We describe here the molecular phenotype of a Trp931Arg mu…
View article: Structure and ion-release mechanism of P <sub>IB-4</sub> -type ATPases
Structure and ion-release mechanism of P <sub>IB-4</sub> -type ATPases Open
Transition metals, such as zinc, are essential micronutrients in all organisms, but also highly toxic in excessive amounts. Heavy-metal transporting P-type (P IB ) ATPases are crucial for homeostasis, conferring cellular detoxification and…
View article: Structure and ion-release mechanism of PIB-4-type ATPases.
Structure and ion-release mechanism of PIB-4-type ATPases. Open
Transition metals, such as zinc, are essential micronutrients in all organisms, but also highly toxic in excessive amounts. Heavy-metal transporting P-type (PIB) ATPases are crucial for homeostasis, conferring cellular detoxification and r…
View article: Bioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper
Bioengineering of non-pathogenic Escherichia coli to enrich for accumulation of environmental copper Open
Heavy metal sequestration from industrial wastes and agricultural soils is a long-standing challenge. This is more critical for copper since copper pollution is hazardous both for the environment and for human health. In this study, we app…
View article: Rationally derived inhibitors of hepatitis C virus (HCV) p7 channel activity reveal prospect for bimodal antiviral therapy
Rationally derived inhibitors of hepatitis C virus (HCV) p7 channel activity reveal prospect for bimodal antiviral therapy Open
Since the 1960s, a single class of agent has been licensed targeting virus-encoded ion channels, or ‘viroporins’, contrasting the success of channel blocking drugs in other areas of medicine. Although resistance arose to these prototypic a…
View article: Author response: Rationally derived inhibitors of hepatitis C virus (HCV) p7 channel activity reveal prospect for bimodal antiviral therapy
Author response: Rationally derived inhibitors of hepatitis C virus (HCV) p7 channel activity reveal prospect for bimodal antiviral therapy Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Since the 1960s, a single class of agent h…
View article: Membrane partitioning of peptide aggregates: coarse-grained molecular dynamics simulations
Membrane partitioning of peptide aggregates: coarse-grained molecular dynamics simulations Open
Coarse-grained molecular dynamics (CGMD) simulation technique (MARTINI force field) is applied to monitor the aggregation of helical peptides representing the transmembrane sequence and its extension of bone marrow stromal cell antigen 2 (…
View article: Membrane partitioning of peptide aggregates: coarse-grained molecular dynamics simulations
Membrane partitioning of peptide aggregates: coarse-grained molecular dynamics simulations Open
Coarse-grained molecular dynamics (CGMD) simulation technique (MARTINI force field) is applied to monitor the aggregation of helical peptides representing the transmembrane sequence and its extension of bone marrow stromal cell antigen 2 (…
View article: Membrane partitioning of peptide aggregates: coarse-grained molecular dynamics simulations
Membrane partitioning of peptide aggregates: coarse-grained molecular dynamics simulations Open
Coarse-grained molecular dynamics (CGMD) simulation technique (MARTINI force field) is applied to monitor the aggregation of helical peptides representing the transmembrane sequence and its extension of bone marrow stromal cell antigen 2 (…
View article: Rationally derived inhibitors of hepatitis C virus (HCV) p7 channel activity reveal prospect for bimodal antiviral therapy
Rationally derived inhibitors of hepatitis C virus (HCV) p7 channel activity reveal prospect for bimodal antiviral therapy Open
Since the 1960s, a single class of agent has been licensed targeting virus-encoded ion channels, or “viroporins”, contrasting the success of channel blocking drugs in other areas of medicine. Although resistance arose to these prototypic a…