Diana Fatykhova
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View article: Diabetes impairs IFNγ-dependent antibacterial defense in the lungs
Diabetes impairs IFNγ-dependent antibacterial defense in the lungs Open
Diabetes mellitus is associated with an increased risk of pneumonia, often caused by so-called typical and atypical pathogens including Streptoccocus pneumoniae and Legionella pneumophila, respectively. Here, we employed a variety of mouse…
View article: Microenvironmental acidification by pneumococcal sugar consumption fosters barrier disruption and immune suppression in the human alveolus
Microenvironmental acidification by pneumococcal sugar consumption fosters barrier disruption and immune suppression in the human alveolus Open
Streptococcus pneumoniae is the most common causative agent of community-acquired pneumonia worldwide. A key pathogenic mechanism that exacerbates severity of disease is the disruption of the alveolar–capillary barrier. However, the specif…
View article: SARS-CoV-2 tropism to intestinal but not gastric epithelial cells is defined by limited ACE2 expression
SARS-CoV-2 tropism to intestinal but not gastric epithelial cells is defined by limited ACE2 expression Open
View article: Correction: State-of-the-art analytical methods of viral infections in human lung organoids
Correction: State-of-the-art analytical methods of viral infections in human lung organoids Open
[This corrects the article DOI: 10.1371/journal.pone.0276115.].
View article: Ex vivo infection model for Francisella using human lung tissue
Ex vivo infection model for Francisella using human lung tissue Open
Introduction Tularemia is mainly caused by Francisella tularensis ( Ft ) subsp. tularensis ( Ftt ) and Ft subsp. holarctica ( Ftt ) in humans and in more than 200 animal species including rabbits and hares. Human clinical manifestations de…
View article: The unremarkable alveolar epithelial glycocalyx: a thorium dioxide-based electron microscopic comparison after heparinase or pneumolysin treatment
The unremarkable alveolar epithelial glycocalyx: a thorium dioxide-based electron microscopic comparison after heparinase or pneumolysin treatment Open
View article: State-of-the-art analytical methods of viral infections in human lung organoids
State-of-the-art analytical methods of viral infections in human lung organoids Open
Human-based organ models can provide strong predictive value to investigate the tropism, virulence, and replication kinetics of viral pathogens. Currently, such models have received widespread attention in the study of SARS-CoV-2 causing t…
View article: Human alveolar progenitors generate dual lineage bronchioalveolar organoids
Human alveolar progenitors generate dual lineage bronchioalveolar organoids Open
View article: Human lungs show limited permissiveness for SARS-CoV-2 due to scarce ACE2 levels but virus-induced expansion of inflammatory macrophages
Human lungs show limited permissiveness for SARS-CoV-2 due to scarce ACE2 levels but virus-induced expansion of inflammatory macrophages Open
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilises the angiotensin-converting enzyme 2 (ACE2) transmembrane peptidase as cellular entry receptor. However, whether SARS-CoV-2 in the alveolar compartment is stri…
View article: Collection:State-of-the-Art Analytics of Viral Infections in Human Lung Organoids v1
Collection:State-of-the-Art Analytics of Viral Infections in Human Lung Organoids v1 Open
Organ models have received widespread attention in the study of SARS-CoV-2, the pathogen causing the current COVID-19 pandemic. Human-based organ models can provide strong predictive value to investigate the tropism, virulence, and replica…
View article: Protein Extraction and Western Blot of Human Lung Organoids v1
Protein Extraction and Western Blot of Human Lung Organoids v1 Open
This protocol describes the protein extraction from human lung organoids followed by western blotting. It gives a detailed description of the preparation of cell lysate from human lung organoids, followed by exact steps to perform a wester…
View article: Functional comparison of MERS-coronavirus lineages reveals increased replicative fitness of the recombinant lineage 5
Functional comparison of MERS-coronavirus lineages reveals increased replicative fitness of the recombinant lineage 5 Open
View article: Microvesicles released from pneumolysin-stimulated lung epithelial cells carry mitochondrial cargo and suppress neutrophil oxidative burst
Microvesicles released from pneumolysin-stimulated lung epithelial cells carry mitochondrial cargo and suppress neutrophil oxidative burst Open
Microvesicles (MVs) are cell-derived extracellular vesicles that have emerged as markers and mediators of acute lung injury (ALI). One of the most common pathogens in pneumonia-induced ALI is Streptococcus pneumoniae (Spn), but the role of…
View article: In vitro screening identifies TRPV4 as target for endothelial barrier stabilization in COVID‐19
In vitro screening identifies TRPV4 as target for endothelial barrier stabilization in COVID‐19 Open
Study objective Endothelial dysfunction and increased microvascular permeability are hallmarks of severe COVID‐19. At present, the extent of endothelial barrier failure and its underlying mechanisms in COVID‑19 remain unclear. We hypothesi…
View article: Plasma mediators in patients with severe COVID-19 cause lung endothelial barrier failure
Plasma mediators in patients with severe COVID-19 cause lung endothelial barrier failure Open
The plasma of COVID-19 patients induces pulmonary microvascular barrier failure, which increases with disease severity. Here, a screening platform to test for plasma mediators and the therapeutic potential of barrier stabilising compounds …
View article: Functional comparison of MERS-coronavirus lineages reveals increased zoonotic potential of the recombinant lineage 5
Functional comparison of MERS-coronavirus lineages reveals increased zoonotic potential of the recombinant lineage 5 Open
Middle East respiratory syndrome coronavirus (MERS-CoV) can cause severe pneumonia in humans. The virus is enzootic in dromedary camels across the Middle East and Africa. It is acquired through animal contact and undergoes limited onward t…
View article: Human Lungs Show Limited Permissiveness for SARS-CoV-2 Due to Scarce ACE2 Levels But Strong Virus-Induced Immune Activation in Alveolar Macrophages
Human Lungs Show Limited Permissiveness for SARS-CoV-2 Due to Scarce ACE2 Levels But Strong Virus-Induced Immune Activation in Alveolar Macrophages Open
View article: Pneumolysin induced mitochondrial dysfunction leads to release of mitochondrial DNA
Pneumolysin induced mitochondrial dysfunction leads to release of mitochondrial DNA Open
View article: Tyk2 as a target for immune regulation in human viral/bacterial pneumonia
Tyk2 as a target for immune regulation in human viral/bacterial pneumonia Open
The severity and lethality of influenza A virus (IAV) infections is frequently aggravated by secondary bacterial pneumonia. However, the mechanisms in human lung tissue that provoke this increase in fatality are unknown and therapeutic imm…
View article: Serotype 1 and 8 Pneumococci Evade Sensing by Inflammasomes in Human Lung Tissue
Serotype 1 and 8 Pneumococci Evade Sensing by Inflammasomes in Human Lung Tissue Open
Streptococcus pneumoniae is a major cause of pneumonia, sepsis and meningitis. The pore-forming toxin pneumolysin is a key virulence factor of S. pneumoniae, which can be sensed by the NLRP3 inflammasome. Among the over 90 serotypes, serot…