Diana Panesso
YOU?
Author Swipe
View article: LiaS-dependent activation of the MadR regulon enables cross-talk between<i>Enterococcus faecalis</i>cell envelope defense systems
LiaS-dependent activation of the MadR regulon enables cross-talk between<i>Enterococcus faecalis</i>cell envelope defense systems Open
Enterococci are gastrointestinal commensals that must defend their cell envelope against antimicrobial peptides derived from the host and other members of the microbiota. The signaling systems LiaFSR and MadRS are pivotal for survival in t…
View article: P-1349. Multi-hospital Dissemination of Ampicillin Resistance Associated with a Frame-shift Deletion in <i>pbp4</i> of Vancomycin-Resistant <i>Enterococcus faecalis</i> (VREfs)
P-1349. Multi-hospital Dissemination of Ampicillin Resistance Associated with a Frame-shift Deletion in <i>pbp4</i> of Vancomycin-Resistant <i>Enterococcus faecalis</i> (VREfs) Open
Background Penicillin (PEN) and ampicillin (AMP) resistance is rare in E. faecalis, and may arise via increased expression of penicillin-binding protein 4 (PBP4), mutations affecting PBP4’s affinity to β-lactams, and rarely β-lactamase pro…
View article: P-889. Therapeutic Failures of High Inoculum Infections with Cefazolin: Role of A Rapid Test and Prevalence of the Cefazolin Inoculum Effect in Methicillin-Susceptible <i>Staphylococcus aureus</i> (MSSA) Bloodstream Infections
P-889. Therapeutic Failures of High Inoculum Infections with Cefazolin: Role of A Rapid Test and Prevalence of the Cefazolin Inoculum Effect in Methicillin-Susceptible <i>Staphylococcus aureus</i> (MSSA) Bloodstream Infections Open
Background Cefazolin (CFZ) remains a key therapy for MSSA bloodstream infections (BSIs), yet some studies suggest poorer outcomes when CFZ is used in the presence of cefazolin inoculum effect (CzIE). We developed a rapid colorimetric test …
View article: P-1342. LiaX Decreases Killing By β-Lactam Antibiotics: Enterococcal Tolerance Revisited
P-1342. LiaX Decreases Killing By β-Lactam Antibiotics: Enterococcal Tolerance Revisited Open
Background Compared to other Firmicutes, enterococci are often tolerant to many antibiotics; combination therapy is used for severe, deep-seated infections. We have previously reported the LiaFSR system, a three-component cell-envelop stre…
View article: P-2083. Validation of the Modified Rapid Test for detection of the Cefazolin Inoculum Effect (CzIE) in bloodstream isolates of Methicillin-<i>Susceptible Staphylococcus aureus</i> (MSSA) from North and Latin America
P-2083. Validation of the Modified Rapid Test for detection of the Cefazolin Inoculum Effect (CzIE) in bloodstream isolates of Methicillin-<i>Susceptible Staphylococcus aureus</i> (MSSA) from North and Latin America Open
Background S. aureus is a major human pathogen and a leading cause of bloodstream infections (BSI). Cefazolin is frequently used for severe deep-seated MSSA infections, as in general it displays similar efficacy and a more favorable side e…
View article: Molecular basis of cell membrane adaptation in daptomycin-resistant Enterococcus faecalis
Molecular basis of cell membrane adaptation in daptomycin-resistant Enterococcus faecalis Open
Daptomycin is a last-resort lipopeptide antibiotic that disrupts cell membrane (CM) and peptidoglycan homeostasis. Enterococcus faecalis has developed a sophisticated mechanism to avoid daptomycin killing by redistributing CM anionic phosp…
View article: Prevalence of the cefazolin inoculum effect (CzIE) in nasal colonizing methicillin-susceptible <i>Staphylococcus aureus</i> in patients from intensive care units in Colombia and use of a modified rapid nitrocefin test for detection
Prevalence of the cefazolin inoculum effect (CzIE) in nasal colonizing methicillin-susceptible <i>Staphylococcus aureus</i> in patients from intensive care units in Colombia and use of a modified rapid nitrocefin test for detection Open
The cefazolin inoculum effect (CzIE) has been associated with poor clinical outcomes in patients with methicillin-susceptible Staphylococcus aureus (MSSA) infections. We aimed to investigate the point prevalence of the CzIE among nasal col…
View article: Prevalence of the Cefazolin Inoculum Effect (CzIE) in Nasal Colonizing Methicillin-Susceptible<i>Staphylococcus aureus</i>in Patients from Intensive Care Units in Colombia and Use of a Modified Rapid Nitrocefin Test for Detection
Prevalence of the Cefazolin Inoculum Effect (CzIE) in Nasal Colonizing Methicillin-Susceptible<i>Staphylococcus aureus</i>in Patients from Intensive Care Units in Colombia and Use of a Modified Rapid Nitrocefin Test for Detection Open
The cefazolin inoculum effect (CzIE) has been associated with poor clinical outcomes in patients with MSSA infections. We aimed to investigate the point prevalence of the CzIE among nasal colonizing MSSA isolates from ICU patients in a mul…
View article: LiaX is a surrogate marker for cell envelope stress and daptomycin non-susceptibility in <i>Enterococcus faecium</i>
LiaX is a surrogate marker for cell envelope stress and daptomycin non-susceptibility in <i>Enterococcus faecium</i> Open
Daptomycin (DAP) is often used as a first-line therapy to treat vancomycin-resistant Enterococcus faecium infections, but emergence of DAP non-susceptibility threatens the effectiveness of this antibiotic. Moreover, current methods to dete…
View article: 735. Vancomycin-Resistant <i>vanB</i>- and <i>vanA/vanB</i>-type <i>Enterococcus faecium</i> Causing Invasive Infections in Adult Patients in Chile
735. Vancomycin-Resistant <i>vanB</i>- and <i>vanA/vanB</i>-type <i>Enterococcus faecium</i> Causing Invasive Infections in Adult Patients in Chile Open
Background Vancomycin-resistant enterococci has increased globally in recent decades, with Enterococcus faecium (VREfm) being the most prevalent species. Although the prevalence of VREfm in Chile is high (∼70%), its molecular epidemiology …
View article: 2176. Cardiolipin Synthases Play Redundant Roles And Are Required for Membrane Remodeling in Daptomycin Resistant <i>Enterococcus faecalis</i>
2176. Cardiolipin Synthases Play Redundant Roles And Are Required for Membrane Remodeling in Daptomycin Resistant <i>Enterococcus faecalis</i> Open
Background Daptomycin (DAP) is a lipopeptide antibiotic targeting anionic phospholipids (APLs) at the division septum. Resistance to DAP (DAP-R) in E. faecalis (Efs) has been associated with activation of the LiaFSR response and redistribu…
View article: 2182. Expression of the MadR regulon in <i>Enterococcus faecalis</i> OG117 is influenced by the presence of the LiaS Histidine Kinase of the LiaFSR pathway
2182. Expression of the MadR regulon in <i>Enterococcus faecalis</i> OG117 is influenced by the presence of the LiaS Histidine Kinase of the LiaFSR pathway Open
Background The MadRS pathway is a two-component signaling (TCS) system which mediates resistance to antimicrobial peptides (AP) and AP-like antibiotics in E. faecalis (Fig. 1). The system is composed of a transmembrane histindine-kinase, M…
View article: 575. Optimization of a Nitrocefin-Based Rapid Test for the Detection of the Cefazolin Inoculum Effect (CzIE) in Methicillin-Susceptible <i>Staphylococcus aureu</i>s
575. Optimization of a Nitrocefin-Based Rapid Test for the Detection of the Cefazolin Inoculum Effect (CzIE) in Methicillin-Susceptible <i>Staphylococcus aureu</i>s Open
Background The cefazolin (Cz) inoculum effect, defined as a Cz MIC ≥16 µg/mL at 107 CFU/mL in MSSA isolates, has been associated with poor outcomes in adult patients with MSSA bacteremia and pediatric patients with osteomyelitis. Broth mic…
View article: 242. Evaluation of PBP4 promoter variation and clinical outcomes in patients with <i>Enterococcus faecalis</i> bacteremia
242. Evaluation of PBP4 promoter variation and clinical outcomes in patients with <i>Enterococcus faecalis</i> bacteremia Open
Background Penicillin-binding protein 4 (PBP4) is a low affinity PBP that has been associated with decreased susceptibility to penicillins in Enterococcus faecalis (Efs). Changes in the promoter region leading to increased expression of th…
View article: LiaX is a surrogate marker for cell-envelope stress and daptomycin non-susceptibility in<i>Enterococcus faecium</i>
LiaX is a surrogate marker for cell-envelope stress and daptomycin non-susceptibility in<i>Enterococcus faecium</i> Open
Daptomycin (DAP) is often used as a first line therapy to treat vancomycin-resistant Enterococcus faecium (VR Efm ) infections but emergence of DAP non-susceptibility threatens the effectiveness of this antibiotic. Moreover, current method…
View article: Molecular Basis of Cell Membrane Adaptation in Daptomycin-Resistant<i>Enterococcus faecalis</i>
Molecular Basis of Cell Membrane Adaptation in Daptomycin-Resistant<i>Enterococcus faecalis</i> Open
Daptomycin is a last-resort lipopeptide antibiotic that disrupts cell membrane (CM) and peptidoglycan homeostasis. Enterococcus faecalis has developed a sophisticated mechanism to avoid daptomycin killing by re-distributing CM anionic phos…
View article: Multisite Detection of Tn <i>1549</i> -Mediated <i>vanB</i> Vancomycin Resistance in Multidrug-Resistant Enterococcus faecalis ST6 in Texas and Florida
Multisite Detection of Tn <i>1549</i> -Mediated <i>vanB</i> Vancomycin Resistance in Multidrug-Resistant Enterococcus faecalis ST6 in Texas and Florida Open
In the United States, vanB -mediated resistance in enterococci is rare. We characterized three sequence type (ST) 6, vancomycin-resistant Enterococcus faecalis isolates causing bacteremia in unique patients in spatiotemporally distinct set…
View article: Characteristics of community-acquired carbapenem-resistant Enterobacterales
Characteristics of community-acquired carbapenem-resistant Enterobacterales Open
Background Community-acquired carbapenem-resistant Enterobacterales (CA-CRE) are an important threat. Methods In CRACKLE-2, we defined patients with CA-CRE as admitted from home, without pre-existing conditions, and a positive culture with…
View article: Accessory Genomes Drive Independent Spread of Carbapenem-Resistant Klebsiella pneumoniae Clonal Groups 258 and 307 in Houston, TX
Accessory Genomes Drive Independent Spread of Carbapenem-Resistant Klebsiella pneumoniae Clonal Groups 258 and 307 in Houston, TX Open
The prevalence of carbapenem-resistant Klebsiella pneumoniae (CR Kp ) infections in nosocomial settings remains a public health challenge. High-risk clones such as clonal group 258 (CG258) are particularly concerning due to their associati…
View article: Accessory Genomes Drive Independent Spread of Carbapenem- Resistant Klebsiella pneumoniae Clonal Groups 258 and 307 in Houston, TX
Accessory Genomes Drive Independent Spread of Carbapenem- Resistant Klebsiella pneumoniae Clonal Groups 258 and 307 in Houston, TX Open
Carbapenem-resistant Klebsiella pneumoniae (CRKp) is an urgent public health threat. Worldwide dissemination of CRKp has been largely attributed to clonal group (CG) 258. However, recent evidence indicates the global emergence of a CRKp CG…
View article: 199. Polymorphisms in Key Regulatory Regions of the <i>bla</i> operon Correlate with the Cefazolin Inoculum Effect in Methicillin-Susceptible <i>Staphylococcus aureus</i> (MSSA)
199. Polymorphisms in Key Regulatory Regions of the <i>bla</i> operon Correlate with the Cefazolin Inoculum Effect in Methicillin-Susceptible <i>Staphylococcus aureus</i> (MSSA) Open
Background The cefazolin inoculum effect (CzIE), defined as Cz minimum inhibitory concentration ≥ 16 µg/ml at high inoculum (HI-MIC), has been associated with poor clinical outcomes in patients with MSSA bacteremia or osteomyelitis. The Cz…
View article: 1275. Dynamics of <i>Enterococcus faecalis</i> Cardiolipin Synthase Gene Expression Reveal Compensatory Roles in Daptomycin Resistance
1275. Dynamics of <i>Enterococcus faecalis</i> Cardiolipin Synthase Gene Expression Reveal Compensatory Roles in Daptomycin Resistance Open
Background Daptomycin (DAP) is a lipopeptide antibiotic targeting membrane anionic phospholipids (APLs) at the division septum, and resistance (DAP-R) has been associated with activation of the E. faecalis (Efs) LiaFSR response and redistr…
View article: Accessory Genomes Drive Independent Spread of Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Clonal Groups 258 and 307
Accessory Genomes Drive Independent Spread of Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Clonal Groups 258 and 307 Open
Background Carbapenem-resistant Klebsiella pneumoniae (CR Kp ) are urgent public health threats. Worldwide dissemination of CR Kp has been largely attributed to the clonal group (CG) 258. However, recent evidence indicates the global emerg…
View article: Dynamics of <i>bla</i> <sub>KPC-2</sub> Dissemination from Non-CG258 <i>Klebsiella pneumoniae</i> to Other <i>Enterobacterales</i> via IncN Plasmids in an Area of High Endemicity
Dynamics of <i>bla</i> <sub>KPC-2</sub> Dissemination from Non-CG258 <i>Klebsiella pneumoniae</i> to Other <i>Enterobacterales</i> via IncN Plasmids in an Area of High Endemicity Open
Carbapenem-resistant Enterobacterales (CRE) pose a significant threat to global public health. The most important mechanism for carbapenem resistance is the production of carbapenemases. Klebsiella pneumoniae carbapenemase (KPC) represents…
View article: Daptomicina combinada con ampicilina, ceftarolina o fosfomicina presenta sinergia in vitro y previene la aparición de resistencia a la daptomicina en enterococos resistentes a vancomicina
Daptomicina combinada con ampicilina, ceftarolina o fosfomicina presenta sinergia in vitro y previene la aparición de resistencia a la daptomicina en enterococos resistentes a vancomicina Open
View article: Actividad IN VITRO de Daptomicina combinada con ceftarolina o FOSFOMICINA FRENTE a Enterococcus faecalis sensible o RESISTENTE a la daptomicina
Actividad IN VITRO de Daptomicina combinada con ceftarolina o FOSFOMICINA FRENTE a Enterococcus faecalis sensible o RESISTENTE a la daptomicina Open
View article: Genomic Epidemiology of Vancomycin-Resistant Enterococcus faecium (VREfm) in Latin America: Revisiting The Global VRE Population Structure
Genomic Epidemiology of Vancomycin-Resistant Enterococcus faecium (VREfm) in Latin America: Revisiting The Global VRE Population Structure Open
View article: Antimicrobial sensing coupled with cell membrane remodeling mediates antibiotic resistance and virulence in <i>Enterococcus faecalis</i>
Antimicrobial sensing coupled with cell membrane remodeling mediates antibiotic resistance and virulence in <i>Enterococcus faecalis</i> Open
Bacteria have developed several evolutionary strategies to protect their cell membranes (CMs) from the attack of antibiotics and antimicrobial peptides (AMPs) produced by the innate immune system, including remodeling of phospholipid conte…
View article: Genomic epidemiology of vancomycin resistant<i>Enterococcus faecium</i>(VR<i>Efm</i>) in Latin America: Revisiting the global VRE population structure
Genomic epidemiology of vancomycin resistant<i>Enterococcus faecium</i>(VR<i>Efm</i>) in Latin America: Revisiting the global VRE population structure Open
The prevalence of vancomycin-resistant Enterococcus faecium varies across geographical regions yet little is known about its population structure in Latin America. Here, we provide a complete genomic characterization of 55 representative L…
View article: 903. Resensitization to β-Lactams in Enterococci Depends on Penicillin-Binding Protein (PBP) Mislocalization and Is Mediated by a Single Protein That Modulates Cell Membrane (CM) Adaptation to Daptomycin (DAP)
903. Resensitization to β-Lactams in Enterococci Depends on Penicillin-Binding Protein (PBP) Mislocalization and Is Mediated by a Single Protein That Modulates Cell Membrane (CM) Adaptation to Daptomycin (DAP) Open
Background DAP disrupts bacterial CM by binding to septal anionic phospholipids (APLs). LiaX, an effector of the LiaFSR stress system, modulates DAP-R by diverting APLs away from the septum. Enterococci are intrinsically resistant to β-lac…