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View article: <i>De novo</i> variants in <i>ATXN7L3</i> lead to developmental delay, hypotonia and distinctive facial features
<i>De novo</i> variants in <i>ATXN7L3</i> lead to developmental delay, hypotonia and distinctive facial features Open
Deubiquitination is crucial for the proper functioning of numerous biological pathways, such as DNA repair, cell cycle progression, transcription, signal transduction and autophagy. Accordingly, pathogenic variants in deubiquitinating enzy…
View article: SUPT3H-less SAGA coactivator can assemble and function without significantly perturbing RNA polymerase II transcription in mammalian cells
SUPT3H-less SAGA coactivator can assemble and function without significantly perturbing RNA polymerase II transcription in mammalian cells Open
Coactivator complexes regulate chromatin accessibility and transcription. SAGA (Spt-Ada-Gcn5 Acetyltransferase) is an evolutionary conserved coactivator complex. The core module scaffolds the entire SAGA complex and adopts a histone octame…
View article: SAGA-Dependent Histone H2Bub1 Deubiquitination Is Essential for Cellular Ubiquitin Balance during Embryonic Development
SAGA-Dependent Histone H2Bub1 Deubiquitination Is Essential for Cellular Ubiquitin Balance during Embryonic Development Open
Ubiquitin (ub) is a small, highly conserved protein widely expressed in eukaryotic cells. Ubiquitination is a post-translational modification catalyzed by enzymes that activate, conjugate, and ligate ub to proteins. Substrates can be modif…
View article: SUPT3H-less SAGA coactivator can assemble and function without significantly perturbing RNA polymerase II transcription in mammalian cells
SUPT3H-less SAGA coactivator can assemble and function without significantly perturbing RNA polymerase II transcription in mammalian cells Open
Coactivator complexes regulate chromatin accessibility and transcription. SAGA (Spt-Ada-Gcn5 Acetyltransferase) is an evolutionary conserved coactivator complex. The core module scaffolds the entire SAGA complex and adopts a histone octame…
View article: Histone H2Bub1 deubiquitylation is essential for mouse development, but does not regulate global RNA polymerase II transcription
Histone H2Bub1 deubiquitylation is essential for mouse development, but does not regulate global RNA polymerase II transcription Open
Co-activator complexes dynamically deposit post-translational modifications (PTMs) on histones, or remove them, to regulate chromatin accessibility and/or to create/erase docking surfaces for proteins that recognize histone PTMs. SAGA (Spt…
View article: Architectural Mediator subunits are differentially essential for global transcription in <i>Saccharomyces cerevisiae</i>
Architectural Mediator subunits are differentially essential for global transcription in <i>Saccharomyces cerevisiae</i> Open
Mediator is a modular coactivator complex involved in the transcription of the majority of RNA polymerase II-regulated genes. However, the degrees to which individual core subunits of Mediator contribute to its activity have been unclear. …
View article: What do the structures of GCN5-containing complexes teach us about their function?
What do the structures of GCN5-containing complexes teach us about their function? Open
Transcription initiation is a major regulatory step in eukaryotic gene expression. It involves the assembly of general transcription factors and RNA polymerase II into a functional pre-initiation complex at core promoters. The degree of ch…
View article: Architectural Mediator subunits are differentially essential for global transcription in yeast
Architectural Mediator subunits are differentially essential for global transcription in yeast Open
Summary The modular Mediator complex is a coactivator of RNA polymerase II transcription. We show that depletion of the main complex scaffold Med14 or the head module scaffold Med17 is lethal and results in global transcriptional downregul…
View article: <em>Saccharomyces cerevisiae</em> Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
<em>Saccharomyces cerevisiae</em> Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity Open
Global defects in RNA polymerase II transcription might be overlooked by transcriptomic studies analyzing steady-state RNA. Indeed, the global decrease in mRNA synthesis has been shown to be compensated by a simultaneous decrease in mRNA d…
View article: Global role for coactivator complexes in RNA polymerase II transcription
Global role for coactivator complexes in RNA polymerase II transcription Open
SAGA and TFIID are related transcription complexes, which were proposed to alternatively deliver TBP at different promoter classes. Recent genome-wide studies in yeast revealed that both complexes are required for the transcription of a va…
View article: Mediator Is Essential for Small Nuclear and Nucleolar RNA Transcription in Yeast
Mediator Is Essential for Small Nuclear and Nucleolar RNA Transcription in Yeast Open
Eukaryotic RNA polymerase II (RNAPII) transcribes mRNA genes and non-protein-coding RNA (ncRNA) genes, including those encoding small nuclear and nucleolar RNAs (sn/snoRNAs). In metazoans, RNAPII transcription of sn/snoRNAs is facilitated …
View article: Homozygous TAF8 mutation in a patient with intellectual disability results in undetectable TAF8 protein, but preserved RNA polymerase II transcription
Homozygous TAF8 mutation in a patient with intellectual disability results in undetectable TAF8 protein, but preserved RNA polymerase II transcription Open
The human general transcription factor TFIID is composed of the TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). In eukaryotic cells, TFIID is thought to nucleate RNA polymerase II (Pol II) preinitiation complex formation o…
View article: Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID
Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID Open
General transcription factor TFIID is a key component of RNA polymerase II transcription initiation. Human TFIID is a megadalton-sized complex comprising TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). TBP binds to core pr…
View article: Author response: Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID
Author response: Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID Open
Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract General transcription factor TFIID is a ke…
View article: Architecture of TAF11/TAF13/TBP complex suggests novel regulatory state in General Transcription Factor TFIID function
Architecture of TAF11/TAF13/TBP complex suggests novel regulatory state in General Transcription Factor TFIID function Open
General transcription factor TFIID is a key component of RNA polymerase II transcription initiation. Human TFIID is a megadalton-sized complex comprising TATA-binding protein (TBP) and 13 TBP-associated factors (TAFs). TBP binds to core pr…
View article: Supporting data for Baptista et al 2017; MOLECULAR-CELL-D-17-00526
Supporting data for Baptista et al 2017; MOLECULAR-CELL-D-17-00526 Open
Western blot images of: - histone marks and RNA Pol II CTD phosphoS2 and phospho S5 for SAGA mutant strains; - cytoplasmic-nuclear fractionation of SPT7-FRB strain; - immunoprecipitation of the SAGA complex in mutant strains.