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View article: Understanding the substrate recognition and catalytic mechanism of 2-O-methyl fucosidases from glycoside hydrolase family 139
Understanding the substrate recognition and catalytic mechanism of 2-O-methyl fucosidases from glycoside hydrolase family 139 Open
Rhamnogalacturonan II is one of the most complex plant cell wall carbohydrates and is composed of 13 different sugars and 21 different glycosidic linkages. It is abundant in fruit and indulgence foods, such as chocolate and wine, making it…
View article: A Bacteroides thetaiotaomicron genetic locus encodes activities consistent with mucin O-glycoprotein processing and N-acetylgalactosamine metabolism
A Bacteroides thetaiotaomicron genetic locus encodes activities consistent with mucin O-glycoprotein processing and N-acetylgalactosamine metabolism Open
The gut microbiota is a key modulator of human health and the status of major diseases including cancer, diabetes and inflammatory bowel disease. Central to microbiota survival is the ability to metabolise complex dietary and host-derived …
View article: Metabolism of hemicelluloses by root-associated <i>Bacteroidota</i> species
Metabolism of hemicelluloses by root-associated <i>Bacteroidota</i> species Open
Bacteroidota species are enriched in the plant microbiome and provide several beneficial functions for their host, including disease suppression. Determining the mechanisms that enable bacteroidota to colonise plant roots may therefore pro…
View article: Hybrid xyloglucan utilisation loci are prevalent among plant-associated Bacteroidota
Hybrid xyloglucan utilisation loci are prevalent among plant-associated Bacteroidota Open
The plant hemicellulose xyloglucan (XyG) is secreted from the roots of numerous plant species, including cereals, and contributes towards soil aggregate formation in terrestrial systems. Whether XyG represents a key nutrient for plant-asso…
View article: A platform for the recombinant production of Group A Streptococcus glycoconjugate vaccines
A platform for the recombinant production of Group A Streptococcus glycoconjugate vaccines Open
Strep A is a human-exclusive bacterial pathogen killing annually more than 500,000 patients, and no current licensed vaccine exists. Strep A bacteria are highly diverse, but all produce an essential, abundant, and conserved surface carbohy…
View article: A genetic locus in the gut microbe<i>Bacteroides thetaiotaomicron</i>encodes activities consistent with mucin-O-glycoprotein processing and plays a critical role in<i>N</i>-acetylgalactosamine metabolism
A genetic locus in the gut microbe<i>Bacteroides thetaiotaomicron</i>encodes activities consistent with mucin-O-glycoprotein processing and plays a critical role in<i>N</i>-acetylgalactosamine metabolism Open
It is increasingly appreciated that members of the gut microbiota are key modulators of human health and the status of major diseases including cancer, diabetes and inflammatory bowel disease. Central to their survival is the ability to me…
View article: Exploring the sequence-function space of microbial fucosidases
Exploring the sequence-function space of microbial fucosidases Open
Microbial α-l-fucosidases catalyse the hydrolysis of terminal α-l-fucosidic linkages with diverse substrate/linkage specificities and can be used in transglycosylation reactions to synthesise oligosaccharides. Based on sequence identity, α…
View article: The human gut symbiont Ruminococcus gnavus shows specificity to blood group A antigen during mucin glycan foraging: Implication for niche colonisation in the gastrointestinal tract
The human gut symbiont Ruminococcus gnavus shows specificity to blood group A antigen during mucin glycan foraging: Implication for niche colonisation in the gastrointestinal tract Open
The human gut symbiont Ruminococcus gnavus displays strain-specific repertoires of glycoside hydrolases (GHs) contributing to its spatial location in the gut. Sequence similarity network analysis identified strain-specific differences in b…
View article: Sulfation of Arabinogalactan Proteins Confers Privileged Nutrient Status to Bacteroides plebeius
Sulfation of Arabinogalactan Proteins Confers Privileged Nutrient Status to Bacteroides plebeius Open
Dietary manipulation of the HGM requires knowledge of how glycans available to this ecosystem are metabolized. The variable structures that decorate the core component of plant AGPs may influence their utilization by specific organisms wit…
View article: Ascertaining the biochemical function of an essential pectin methylesterase in the gut microbe Bacteroides thetaiotaomicron
Ascertaining the biochemical function of an essential pectin methylesterase in the gut microbe Bacteroides thetaiotaomicron Open
Pectins are a major dietary nutrient source for the human gut microbiota. The prominent gut microbe Bacteroides thetaiotaomicron was recently shown to encode the founding member (BT1017) of a new family of pectin methylesterases essential …
View article: Metabolism of multiple glycosaminoglycans by Bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus
Metabolism of multiple glycosaminoglycans by Bacteroides thetaiotaomicron is orchestrated by a versatile core genetic locus Open
The human gut microbiota (HGM), which is critical to human health, utilises complex glycans as its major carbon source. Glycosaminoglycans represent an important, high priority, nutrient source for the HGM. Pathways for the metabolism of v…
View article: Single cell fluorescence imaging of glycan uptake by intestinal bacteria
Single cell fluorescence imaging of glycan uptake by intestinal bacteria Open
Microbes in the intestines of mammals degrade dietary glycans for energy and growth. The pathways required for polysaccharide utilization are functionally diverse; moreover, they are unequally dispersed between bacterial genomes. Hence, as…
View article: Single cell fluorescence imaging of glycan uptake by intestinal bacteria
Single cell fluorescence imaging of glycan uptake by intestinal bacteria Open
Microbes in the intestines of mammals degrade dietary glycans for energy and growth. The pathways required for polysaccharide utilization are functionally diverse; moreover, they are unequally dispersed between bacterial genomes. Hence, as…
View article: The human gut microbe Bacteroides thetaiotaomicron encodes the founding member of a novel glycosaminoglycan-degrading polysaccharide lyase family PL29
The human gut microbe Bacteroides thetaiotaomicron encodes the founding member of a novel glycosaminoglycan-degrading polysaccharide lyase family PL29 Open
Glycosaminoglycans (GAGs) and GAG-degrading enzymes have wide-ranging applications in the medical and biotechnological industries. The former are also an important nutrient source for select species of the human gut microbiota (HGM), a key…
View article: Biochemistry of complex glycan depolymerisation by the human gut microbiota
Biochemistry of complex glycan depolymerisation by the human gut microbiota Open
The human gut microbiota (HGM) makes an important contribution to health and disease. It is a complex microbial community of trillions of microbes with a majority of its members represented within two phyla, the Bacteroidetes and Firmicute…
View article: Target highlights from the first post‐PSI CASP experiment (CASP12, May–August 2016)
Target highlights from the first post‐PSI CASP experiment (CASP12, May–August 2016) Open
The functional and biological significance of the selected CASP12 targets are described by the authors of the structures. The crystallographers discuss the most interesting structural features of the target proteins and assess whether thes…
View article: How members of the human gut microbiota overcome the sulfation problem posed by glycosaminoglycans
How members of the human gut microbiota overcome the sulfation problem posed by glycosaminoglycans Open
Significance The major nutrients available to the human microbiota are complex carbohydrates. Host glycans are important to this microbial community, particularly when dietary carbohydrates are scarce. The host glycans heparin and heparan …