Diego B. Nóbrega
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View article: Differences in milk microbiota between healthy cows and those with recurring Klebsiella mastitis
Differences in milk microbiota between healthy cows and those with recurring Klebsiella mastitis Open
Klebsiella spp. infections are an important cause of severe clinical mastitis and recurrent infections, resulting in a poor response to antimicrobial agents and causing substantial economic impacts on the dairy industry. Therefore, investi…
View article: Infodemiology of public sentiment toward the measles vaccine in Canada: A google trends and health belief model–based analysis, 2025
Infodemiology of public sentiment toward the measles vaccine in Canada: A google trends and health belief model–based analysis, 2025 Open
Measles remains a public health threat in Canada despite vaccine availability, making effective risk communication essential to deal with vaccine hesitancy. This study, conducted from July through September 2025, analyzed Google Trends dat…
View article: Analyzing Public Google Search Interest in Measles Within Canada: Identifying Key Moments for Targeted Risk Communication
Analyzing Public Google Search Interest in Measles Within Canada: Identifying Key Moments for Targeted Risk Communication Open
We analyzed Google Trends data on measles-related searches in Canada from January 1 to May 21, 2025; web, news, and YouTube search trends increased significantly across provinces (all P values were <.05), aligning with rising case numbers.…
View article: <i>Salmonella</i> Dublin in dairy cattle: Review of state of the science.
<i>Salmonella</i> Dublin in dairy cattle: Review of state of the science. Open
Salmonella Dublin is an emerging pathogen and action is necessary to control its spread.
View article: Analyzing Public Google Search Interest on Measles in Canada: Identifying Key Moments for Targeted Risk Communication (Preprint)
Analyzing Public Google Search Interest on Measles in Canada: Identifying Key Moments for Targeted Risk Communication (Preprint) Open
UNSTRUCTURED We analyzed Google Trends data on measles-related searches in Canada from January 1 to March 26, 2025. Web, news, and YouTube search trends increased significantly across provinces, aligning with rising case numbers. Our find…
View article: P-1805. Identifying Hospital Antimicrobial Usage Trends Using Wastewater Based Surveillance
P-1805. Identifying Hospital Antimicrobial Usage Trends Using Wastewater Based Surveillance Open
Background Hospitals represent ideal locations for developing wastewater (WW) surveillance for antibiotics (Abx), owing to the high frequency of Abx use and robust record-keeping. To investigate this technology as a potentially useful stew…
View article: Virulence of Bacteria Causing Mastitis in Dairy Cows: A Literature Review
Virulence of Bacteria Causing Mastitis in Dairy Cows: A Literature Review Open
Bovine mastitis, a prevalent disease in dairy farms, exerts a profound negative influence on both the health and productivity of dairy cattle, leading to substantial economic losses for the dairy industry. The disease is associated with di…
View article: Antimicrobial Resistance and Use on Chinese Dairy Farms: Awareness and Opinions Regarding Selective Treatments of Farm Managers
Antimicrobial Resistance and Use on Chinese Dairy Farms: Awareness and Opinions Regarding Selective Treatments of Farm Managers Open
Background: In China’s expanding dairy industry, a lack of oversight regarding antimicrobial use and increasing antimicrobial resistance are evident. Selective treatments of dairy cows for clinical mastitis or dry cow therapy are proposed …
View article: Genes associated with fitness and disease severity in the pan-genome of mastitis-associated Escherichia coli
Genes associated with fitness and disease severity in the pan-genome of mastitis-associated Escherichia coli Open
Introduction Bovine mastitis caused by Escherichia coli compromises animal health and inflicts substantial product losses in dairy farming. It may manifest as subclinical through severe acute disease and can be transient or persistent in n…
View article: Impact of carbon dioxide concentrations on laboratory sensitivity of <i>Mycoplasma</i> species isolated from dairy cows
Impact of carbon dioxide concentrations on laboratory sensitivity of <i>Mycoplasma</i> species isolated from dairy cows Open
Conventional Mycoplasma spp. diagnostics involve culture, often considered the gold standard in diagnostic test evaluation. However, culture protocols lack empirical derivation and primarily adhere to National Mastitis Council recommendati…
View article: Biological and genomic characterization of 4 novel bacteriophages isolated from sewage or the environment using non-aureus Staphylococci strains
Biological and genomic characterization of 4 novel bacteriophages isolated from sewage or the environment using non-aureus Staphylococci strains Open
Non-aureus staphylococci (NAS) are an essential group of bacteria causing antimicrobial resistant intramammary infections in livestock, particularly dairy cows. Therefore, bacteriophages emerge as a potent bactericidal agent for NAS mastit…
View article: Extended-Spectrum Beta-Lactamase-Producing Escherichia coli and Other Antimicrobial-Resistant Gram-Negative Pathogens Isolated from Bovine Mastitis: A One Health Perspective
Extended-Spectrum Beta-Lactamase-Producing Escherichia coli and Other Antimicrobial-Resistant Gram-Negative Pathogens Isolated from Bovine Mastitis: A One Health Perspective Open
Antimicrobial resistance (AMR) poses an imminent threat to global public health, driven in part by the widespread use of antimicrobials in both humans and animals. Within the dairy cattle industry, Gram-negative coliforms such as Escherich…
View article: Prevalence and spatial distribution of infectious diseases of dairy cattle in Ontario, Canada
Prevalence and spatial distribution of infectious diseases of dairy cattle in Ontario, Canada Open
We investigated the prevalence and spatial distribution of selected pathogens associated with infectious diseases of dairy cattle in Ontario, Canada. The cross-sectional study surveyed bulk tank milk for antibodies against bovine leukemia …
View article: Differences in milk microbiota between healthy cows and cows with recurring<i>Klebsiella</i>mastitis
Differences in milk microbiota between healthy cows and cows with recurring<i>Klebsiella</i>mastitis Open
Klebsiella spp. infections continue to have a significant economic impact on the dairy industry, being an important cause of severe clinical mastitis, recurrent infections, and demonstrating poor response to antimicrobials. It is, therefor…
View article: Accuracy of an AI-based automated plate reading mobile application for the identification of clinical mastitis-causing pathogens in chromogenic culture media
Accuracy of an AI-based automated plate reading mobile application for the identification of clinical mastitis-causing pathogens in chromogenic culture media Open
Using on-farm microbiological culture (OFC), based on chromogenic culture media, enables the identification of mastitis causing pathogens in about 24 h, allows rapid decision making on selective treatment and control management measures of…
View article: Comparison of laboratory methods for diagnosis of human visceral leishmaniasis in the Center-West Region of São Paulo State, Brazil
Comparison of laboratory methods for diagnosis of human visceral leishmaniasis in the Center-West Region of São Paulo State, Brazil Open
Visceral leishmaniasis (VL) is a neglected disease and represents an important public health problem. The present study aimed to compare the conventional laboratory methods with the molecular method for VL diagnosis in human blood samples.…
View article: Comparative genomic analyses of Klebsiella pneumoniae K57 capsule serotypes isolated from bovine mastitis in China
Comparative genomic analyses of Klebsiella pneumoniae K57 capsule serotypes isolated from bovine mastitis in China Open
Klebsiella pneumoniae can cause severe clinical mastitis in dairy cows, with K. pneumoniae type K57 (K57-KP) being the most common capsular serotype. To identify virulence factors and antimicrobial-resistance (AMR) genes of K57-KP with var…
View article: Genes associated with fitness and disease severity in the pan-genome of mastitis-associated<i>Escherichia coli</i>
Genes associated with fitness and disease severity in the pan-genome of mastitis-associated<i>Escherichia coli</i> Open
Bovine mastitis caused by Escherichia coli may manifest as subclinical through severe acute disease and can be transient or persistent in nature. Little is known about bacterial factors that impact clinical outcomes or allow some strains t…
View article: A scoping review of the testing of bulk tank milk to detect nonbacterial pathogens or herd exposure to nonbacterial pathogens in dairy cattle
A scoping review of the testing of bulk tank milk to detect nonbacterial pathogens or herd exposure to nonbacterial pathogens in dairy cattle Open
In this scoping review, we characterized the literature reporting on the testing of bulk milk samples to detect microorganisms other than bacteria that can cause diseases in dairy cattle, including viruses, helminths, algae, and protozoa. …
View article: Invited review: Selective treatment of clinical mastitis in dairy cattle
Invited review: Selective treatment of clinical mastitis in dairy cattle Open
Treatment of clinical mastitis (CM) and use of antimicrobials for dry cow therapy are responsible for the majority of animal-defined daily doses of antimicrobial use (AMU) on dairy farms. However, advancements made in the last decade have …
View article: Molecular Epidemiology of Global Carbapenemase-Producing <i>Citrobacter</i> spp. (2015–2017)
Molecular Epidemiology of Global Carbapenemase-Producing <i>Citrobacter</i> spp. (2015–2017) Open
Citrobacter spp. are gaining recognition as important causes of hospital-acquired infections in humans. Among Citrobacter spp., carbapenemase-producing strains are cause of utmost concern to health care services globally due to their abili…
View article: A scoping review of the testing of bulk milk to detect infectious diseases of dairy cattle: Diseases caused by bacteria
A scoping review of the testing of bulk milk to detect infectious diseases of dairy cattle: Diseases caused by bacteria Open
Testing of bulk milk (BM) samples is a convenient, cost-effective strategy that can easily be implemented as part of disease surveillance programs on dairy farms. Here, we performed a scoping review to summarize the literature reporting on…
View article: Genomic Epidemiology of Global Carbapenemase-Producing <i>Escherichia coli,</i> 2015–2017
Genomic Epidemiology of Global Carbapenemase-Producing <i>Escherichia coli,</i> 2015–2017 Open
We describe the global molecular epidemiology of 229 carbapenemase-producing Escherichia coli in 36 countries during 2015-2017. Common carbapenemases were oxacillinase (OXA) 181 (23%), New Delhi metallo-β-lactamase (NDM) 5 (20%), OXA-48 (1…
View article: Prevalence, Risk Factors, and Antimicrobial Resistance Profile of Respiratory Pathogens Isolated From Suckling Beef Calves to Reprocessing at the Feedlot: A Longitudinal Study
Prevalence, Risk Factors, and Antimicrobial Resistance Profile of Respiratory Pathogens Isolated From Suckling Beef Calves to Reprocessing at the Feedlot: A Longitudinal Study Open
Here, we investigated the prevalence and risk factors for the presence of Histophilus somni, Mannheimia haemolytica, Mycoplasma bovis , and Pasteurella multocida in the respiratory tract of calves from the spring processing to the reproces…
View article: Knowledge Gaps in the Understanding of Antimicrobial Resistance in Canada
Knowledge Gaps in the Understanding of Antimicrobial Resistance in Canada Open
Current limitations in the understanding and control of antimicrobial resistance (AMR) in Canada are described through a comprehensive review focusing on: (1) treatment optimization; (2) surveillance of antimicrobial use and AMR; and (3) p…
View article: Use of bulk milk samples to detect infectious diseases of dairy cattle: Protocol for a scoping review
Use of bulk milk samples to detect infectious diseases of dairy cattle: Protocol for a scoping review Open
Background: In spite of the growing interest in the use of aggregate samples such as bulk tank milk to detect endemic and emerging diseases of dairy cattle, there is no synthesis of the literature reporting in the testing of bulk milk as w…
View article: Virulence profiles of Klebsiella pneumoniae isolated from 2 large dairy farms in China
Virulence profiles of Klebsiella pneumoniae isolated from 2 large dairy farms in China Open
We recently reported on the diversity of Klebsiella pneumoniae isolated from dairy herds in China. In our previous work, isolates from subclinical mastitis (SCM) had lower indices of diversity when compared with bacteria from other sources…
View article: Non-aureus Staphylococci and Bovine Udder Health: Current Understanding and Knowledge Gaps
Non-aureus Staphylococci and Bovine Udder Health: Current Understanding and Knowledge Gaps Open
Despite considerable efforts to control bovine mastitis and explain its causes, it remains the most costly and common disease of dairy cattle worldwide. The role and impact of non- aureus staphylococci (NAS) in udder health are not entirel…
View article: Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds
Molecular characterization of antimicrobial resistance in Klebsiella pneumoniae isolated from Brazilian dairy herds Open
In this observational study, phenotypic and genotypic patterns of antimicrobial resistance (AMR) in Klebsiella pneumoniae isolated from intramammary infections, clinical mastitis, fresh feces, rectal swabs, animal hindlimbs, and bulk tank …
View article: Supplemental table 1
Supplemental table 1 Open
Supplemental table for jds.2020-19569