Dietmar Hammerschmid
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View article: Droplet microfluidic hydrogen/deuterium exchange for investigating protein dynamics with millisecond precision
Droplet microfluidic hydrogen/deuterium exchange for investigating protein dynamics with millisecond precision Open
Hydrogen/deuterium exchange (HDX) methods for studying protein dynamics would benefit from millisecond-scale incubations to probe intrinsically disordered proteins, highly dynamic regions and conformation changes. Here we investigate dropl…
View article: Mg2+-dependent mechanism of environmental versatility in a multidrug efflux pump
Mg2+-dependent mechanism of environmental versatility in a multidrug efflux pump Open
Tripartite resistance nodulation and cell division multidrug efflux pumps span the periplasm and are major drivers of multidrug resistance among gram-negative bacteria. Cations, such as Mg2+, become concentrated within the periplasm and, i…
View article: Mg<sup>2+</sup>-dependent mechanism of environmental versatility in a multidrug efflux pump
Mg<sup>2+</sup>-dependent mechanism of environmental versatility in a multidrug efflux pump Open
Tripartite resistance nodulation and cell division multidrug efflux pumps span the periplasm and are a major driver of multidrug resistance among Gram-negative bacteria. The periplasm provides a distinct environment between the inner and o…
View article: Chromatographic Phospholipid Trapping for Automated H/D Exchange Mass Spectrometry of Membrane Protein–Lipid Assemblies
Chromatographic Phospholipid Trapping for Automated H/D Exchange Mass Spectrometry of Membrane Protein–Lipid Assemblies Open
Lipid interactions modulate the function, folding, structure, and organization of membrane proteins. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) has emerged as a useful tool to understand the structural dynamics of these protein…
View article: Structural mass spectrometry approaches to understand multidrug efflux systems
Structural mass spectrometry approaches to understand multidrug efflux systems Open
Multidrug efflux pumps are ubiquitous across both eukaryotes and prokaryotes, and have major implications in antimicrobial and multidrug resistance. They reside within cellular membranes and have proven difficult to study owing to their hy…
View article: Conformational restriction shapes inhibition of a multidrug efflux adaptor protein
Conformational restriction shapes inhibition of a multidrug efflux adaptor protein Open
Membrane efflux pumps play a major role in bacterial multidrug resistance. The tripartite multidrug efflux pump system from Escherichia coli , AcrAB-TolC, is a target for inhibition to lessen resistance development and restore antibiotic e…
View article: Chromatographic phospholipid trapping for automated H/D exchange mass spectrometry analysis of membrane protein-lipid assemblies
Chromatographic phospholipid trapping for automated H/D exchange mass spectrometry analysis of membrane protein-lipid assemblies Open
Lipid interactions modulate the function, folding, structure, and organization of membrane proteins. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) has emerged as a useful tool to understand the structural dynamics of these protein…
View article: Structural dynamics in the evolution of SARS-CoV-2 spike glycoprotein
Structural dynamics in the evolution of SARS-CoV-2 spike glycoprotein Open
SARS-CoV-2 spike glycoprotein mediates receptor binding and subsequent membrane fusion. It exists in a range of conformations, including a closed state unable to bind the ACE2 receptor, and an open state that does so but displays more expo…
View article: Enhanced oligomerization of full-length RAGE by synergy of the interaction of its domains
Enhanced oligomerization of full-length RAGE by synergy of the interaction of its domains Open
The pattern recognition receptor RAGE (receptor for advanced glycation end-products) transmits proinflammatory signals in several inflammation-related pathological states, including vascular diseases, cancer, neurodegeneration and diabetes…
View article: Synergistic Effects of Melittin and Plasma Treatment: A Promising Approach for Cancer Therapy
Synergistic Effects of Melittin and Plasma Treatment: A Promising Approach for Cancer Therapy Open
Melittin (MEL), a small peptide component of bee venom, has been reported to exhibit anti-cancer effects in vitro and in vivo. However, its clinical applicability is disputed because of its non-specific cytotoxicity and haemolytic activity…
View article: The effect of reactive oxygen and nitrogen species on the structure of cytoglobin: A potential tumor suppressor
The effect of reactive oxygen and nitrogen species on the structure of cytoglobin: A potential tumor suppressor Open
Many current anti-cancer therapies rely on increasing the intracellular reactive oxygen and nitrogen species (RONS) contents with the aim to induce irreparable damage, which subsequently results in tumor cell death. A novel tool in cancer …