Dietmar Reusch
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View article: Glycosylation‐Dependent Stability of Human Pentraxin‐2 Revealed by Surface‐Induced Dissociation and Ion Mobility Mass Spectrometry
Glycosylation‐Dependent Stability of Human Pentraxin‐2 Revealed by Surface‐Induced Dissociation and Ion Mobility Mass Spectrometry Open
Pentraxin‐2 (PTX‐2), also known as Serum Amyloid P component, functions as an immunoregulatory glycoprotein and plays a crucial role in fibrotic diseases such as idiopathic pulmonary fibrosis (IPF). Desialylation of PTX‐2 has recently been…
View article: Direct glycosylation analysis of intact monoclonal antibodies combining ESI MS of glycoforms and MALDI-in source decay MS of glycan fragments
Direct glycosylation analysis of intact monoclonal antibodies combining ESI MS of glycoforms and MALDI-in source decay MS of glycan fragments Open
View article: Editorial: New functional separation and analysis technologies utilizing human Fc receptors
Editorial: New functional separation and analysis technologies utilizing human Fc receptors Open
Keywords: Fc receptors, analysis technologies, molecular innate immunity, effector cells, adaptive and innate immunity
View article: Function-structure approach reveals novel insights on the interplay of Immunoglobulin G 1 proteoforms and Fc gamma receptor IIa allotypes
Function-structure approach reveals novel insights on the interplay of Immunoglobulin G 1 proteoforms and Fc gamma receptor IIa allotypes Open
Human Fc gamma receptor IIa (FcγRIIa) or CD32a has two major allotypes with a single amino acid difference at position 131 (histidine or arginine). Differences in FcγRIIa allotypes are known to impact immunological responses such as the cl…
View article: 10th antibody industrial symposium: new developments in antibody and adoptive cell therapies
10th antibody industrial symposium: new developments in antibody and adoptive cell therapies Open
The annual "Antibody Industrial Symposium", co-organized by LabEx MAbImprove and MabDesign, held its 10th anniversary edition in Montpellier, France, on June 28-29, 2022. The meeting focused on new results and concepts in antibody engineer…
View article: Mass spectrometry-based multi-attribute method in protein therapeutics product quality monitoring and quality control
Mass spectrometry-based multi-attribute method in protein therapeutics product quality monitoring and quality control Open
The multi-attribute method (MAM), a liquid chromatography-mass spectrometry (LC-MS)-based peptide mapping method, has gained increased interest and applications in the biopharmaceutical industry. MAM can, in one method, provide targeted qu…
View article: Characterization of high-molecular weight by-products in the production of a trivalent bispecific 2+1 heterodimeric antibody
Characterization of high-molecular weight by-products in the production of a trivalent bispecific 2+1 heterodimeric antibody Open
The development of increasingly complex antibody formats, such as bispecifics, can lead to the formation of increasingly complex high- and low-molecular-weight by-products. Here, we focus on the characterization of high molecular weight sp…
View article: Identification of Hetero-aggregates in Antibody Co-formulations by Multi-dimensional Liquid Chromatography Coupled to Mass Spectrometry
Identification of Hetero-aggregates in Antibody Co-formulations by Multi-dimensional Liquid Chromatography Coupled to Mass Spectrometry Open
Antibody combination therapies have become viable therapeutic treatment options for certain severe diseases such as cancer. The co-formulation production approach is intrinsically associated with more complex drug product variant profiles …
View article: Compliance and Regulatory Considerations for the Implementation of the Multi-Attribute-Method by Mass Spectrometry in a Quality Control Laboratory
Compliance and Regulatory Considerations for the Implementation of the Multi-Attribute-Method by Mass Spectrometry in a Quality Control Laboratory Open
View article: Comprehensive Multidimensional Liquid Chromatography–Mass Spectrometry for the Characterization of Charge Variants of a Bispecific Antibody
Comprehensive Multidimensional Liquid Chromatography–Mass Spectrometry for the Characterization of Charge Variants of a Bispecific Antibody Open
Identification and further characterization of antibody charge variants is a crucial step during biopharmaceutical drug development, particularly with regard to the increasing complexity of novel antibody formats. As a standard analytical …
View article: Clearance of therapeutic antibody glycoforms after subcutaneous and intravenous injection in a porcine model
Clearance of therapeutic antibody glycoforms after subcutaneous and intravenous injection in a porcine model Open
A relatively low clearance is one of the prominent favorable features of immunoglobulin G1-based therapeutic monoclonal antibodies (mAbs). Various studies have observed differential clearance of mAb glycoforms, including oligomannose glyco…
View article: Affinity capillary electrophoresis – mass spectrometry permits direct binding assessment of IgG and FcγRIIa in a glycoform-resolved manner
Affinity capillary electrophoresis – mass spectrometry permits direct binding assessment of IgG and FcγRIIa in a glycoform-resolved manner Open
The impact of antibody glycoforms on FcγRIIa activation and immune responses is poorly understood. Yet, glycoform binding assessment remains one of the major analytical challenges requiring long enrichment or glycoengineering steps. Here, …
View article: Detailed Analytical Characterization of a Bispecific IgG1 CrossMab Antibody of the Knob-into-Hole Format Applying Various Stress Conditions Revealed Pronounced Stability
Detailed Analytical Characterization of a Bispecific IgG1 CrossMab Antibody of the Knob-into-Hole Format Applying Various Stress Conditions Revealed Pronounced Stability Open
In recent years, a variety of new antibody formats have been developed. One of these formats allows the binding of one type of antibody to two different epitopes. This can for example be achieved by introduction of the "knob-into-hole" for…
View article: Affinity Capillary Electrophoresis–Mass Spectrometry as a Tool to Unravel Proteoform-Specific Antibody–Receptor Interactions
Affinity Capillary Electrophoresis–Mass Spectrometry as a Tool to Unravel Proteoform-Specific Antibody–Receptor Interactions Open
Monoclonal antibody (mAb) pharmaceuticals consist of a plethora of different proteoforms with different functional characteristics, including pharmacokinetics and pharmacodynamics, requiring their individual assessment. Current binding tec…
View article: Glycoform analysis of intact erythropoietin by MALDI FT-ICR mass spectrometry
Glycoform analysis of intact erythropoietin by MALDI FT-ICR mass spectrometry Open
View article: High sensitivity glycomics in biomedicine
High sensitivity glycomics in biomedicine Open
Many analytical challenges in biomedicine arise from the generally high heterogeneity and complexity of glycan‐ and glycoconjugate‐containing samples, which are often only available in minute amounts. Therefore, highly sensitive workflows …
View article: Discrepancies between High-Resolution Native and Glycopeptide-Centric Mass Spectrometric Approaches: A Case Study into the Glycosylation of Erythropoietin Variants
Discrepancies between High-Resolution Native and Glycopeptide-Centric Mass Spectrometric Approaches: A Case Study into the Glycosylation of Erythropoietin Variants Open
Glycosylation represents a critical quality attribute modulating a myriad of physiochemical properties and effector functions of biotherapeutics. Furthermore, a rising landscape of glycosylated biotherapeutics including biosimilars, biobet…
View article: Fc gamma receptor IIIb binding of individual antibody proteoforms resolved by affinity chromatography–mass spectrometry
Fc gamma receptor IIIb binding of individual antibody proteoforms resolved by affinity chromatography–mass spectrometry Open
The crystallizable fragment (Fc) of immunoglobulin G (IgG) activates key immunological responses by interacting with Fc gamma receptors (FcɣR). FcɣRIIIb contributes to neutrophil activation and is involved in antibody-dependent cellular cy…
View article: Glycoform-resolved pharmacokinetic studies in a rat model employing glycoengineered variants of a therapeutic monoclonal antibody
Glycoform-resolved pharmacokinetic studies in a rat model employing glycoengineered variants of a therapeutic monoclonal antibody Open
Good pharmacokinetic (PK) behavior is a key prerequisite for sufficient efficacy of therapeutic monoclonal antibodies (mAbs). Fc glycosylation is a critical quality attribute (CQA) of mAbs, due to its impact on stability and effector funct…
View article: Anion exchange chromatography – Mass spectrometry for monitoring multiple quality attributes of erythropoietin biopharmaceuticals
Anion exchange chromatography – Mass spectrometry for monitoring multiple quality attributes of erythropoietin biopharmaceuticals Open
View article: Intact and subunit-specific analysis of bispecific antibodies by sheathless CE-MS
Intact and subunit-specific analysis of bispecific antibodies by sheathless CE-MS Open
View article: Cysteine Aminoethylation Enables the Site-Specific Glycosylation Analysis of Recombinant Human Erythropoietin using Trypsin
Cysteine Aminoethylation Enables the Site-Specific Glycosylation Analysis of Recombinant Human Erythropoietin using Trypsin Open
Recombinant human erythropoietin (rhEPO) is an important biopharmaceutical for which glycosylation is a critical quality attribute. Therefore, robust analytical methods are needed for the in-depth characterization of rhEPO glycosylation. C…
View article: Interaction analysis of glycoengineered antibodies with CD16a: a native mass spectrometry approach
Interaction analysis of glycoengineered antibodies with CD16a: a native mass spectrometry approach Open
Minor changes in the quality of biologically manufactured monoclonal antibodies (mAbs) can affect their bioactivity and efficacy. One of the most important variations concerns the N-glycosylation pattern, which directly affects an anti-tum…
View article: Monitoring glycation levels of a bispecific monoclonal antibody at subunit level by ultrahigh-resolution MALDI FT-ICR mass spectrometry
Monitoring glycation levels of a bispecific monoclonal antibody at subunit level by ultrahigh-resolution MALDI FT-ICR mass spectrometry Open
Bispecific monoclonal antibodies (BsAbs) are engineered proteins with multiple functionalities and properties. The "bi-specificity" of these complex biopharmaceuticals is a key characteristic for the development of novel and more effective…
View article: The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange
The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange Open
The usefulness of higher-order structural information provided by hydrogen/deuterium exchange-mass spectrometry (H/DX-MS) for the structural impact analyses of chemical and post-translational antibody modifications has been demonstrated in…
View article: Effects of Sialic Acid Linkage on Antibody-Fragment Crystallizable Receptor Binding and Antibody Dependent Cytotoxicity Depend on Levels of Fucosylation/Bisecting
Effects of Sialic Acid Linkage on Antibody-Fragment Crystallizable Receptor Binding and Antibody Dependent Cytotoxicity Depend on Levels of Fucosylation/Bisecting Open
Aim: Fragment crystallizable (Fc) glycosylation of immunoglobulin G-type monoclonal antibodies applied to therapeutic applications is regarded a critical quality attribute and can influence bioactivity, pharmacokinetics and/or immun…
View article: Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry
Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry Open
Determination of the impact of individual antibody glycoforms on FcɣRIIIa affinity, and consequently antibody-dependent cell-mediated cytotoxicity (ADCC) previously required high purity glycoengineering. We hyphenated FcɣRIIIa affinity chr…
View article: Design–functionality relationships for adhesion/growth-regulatory galectins
Design–functionality relationships for adhesion/growth-regulatory galectins Open
Significance Glycan-lectin recognition is vital to embryogenesis, and progression in common diseases such as cancer and osteoarthritis. However, the rules governing specificity for the functional pairing and postbinding events are not yet …
View article: Assessment of susceptible chemical modification sites of trastuzumab and endogenous human immunoglobulins at physiological conditions
Assessment of susceptible chemical modification sites of trastuzumab and endogenous human immunoglobulins at physiological conditions Open
View article: Chicken GRIFIN: Structural characterization in crystals and in solution
Chicken GRIFIN: Structural characterization in crystals and in solution Open