Digantkumar Chapla
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View article: Chemoenzymatic synthesis of N-linked glycan receptors of H1N1 influenza virus on human airway epithelial cells
Chemoenzymatic synthesis of N-linked glycan receptors of H1N1 influenza virus on human airway epithelial cells Open
Attachment of human influenza virus to the surface of airway epithelial cells is mediated by the hemagglutinin (HA) that binds to Neu5Acα2-6Gal-terminated glycans referred to as human-type receptors. Numerous reports suggest that current H…
View article: Biochemical and structural characterization of glycosyltransferase family 61 proteins reveal a key determinant of sugar donor specificity
Biochemical and structural characterization of glycosyltransferase family 61 proteins reveal a key determinant of sugar donor specificity Open
Xylan, the most abundant non-cellulosic polymer in plant cell walls, is structurally diverse, especially in grasses where it is heavily substituted with arabinofuranose and further modified by various residues. Common substitutions across …
View article: Siglec-targeted liposomes to identify sialoglycans present on fungal pathogens
Siglec-targeted liposomes to identify sialoglycans present on fungal pathogens Open
The sialic acid Ig-like lectins Siglec-3 and Siglec-15 are pathogen receptors that bind sialic acid-modified glycoproteins, best characterized in metastatic cancers. Because fungi produce sialoglycans and sialo-glycoproteins, we wondered i…
View article: Enforced E-selectin ligand installation enhances homing and efficacy of adoptively transferred T cells
Enforced E-selectin ligand installation enhances homing and efficacy of adoptively transferred T cells Open
Adoptive T-cell transfer has revolutionized the treatment of hematological malignancies. However, this approach has had very limited success in treating solid tumors, largely due to inadequate infiltration of vascularly administered T cell…
View article: TBR is an RG-I rhamnose O-acetyltransferase required for epidermal cell adhesion in Arabidopsis thaliana
TBR is an RG-I rhamnose O-acetyltransferase required for epidermal cell adhesion in Arabidopsis thaliana Open
Pectins are enriched in primary cell walls and the middle lamella, determining cell-to-cell adhesion. O-acetylation of pectin backbones influences their physicochemical properties and plays a role in plant development and interactions with…
View article: Chemoenzymatic Synthesis of Keratan Sulfate Oligosaccharides Using UDP-Galactose-6-aldehyde To Control Sulfation at Galactosides
Chemoenzymatic Synthesis of Keratan Sulfate Oligosaccharides Using UDP-Galactose-6-aldehyde To Control Sulfation at Galactosides Open
Keratan sulfate (KS) is a highly complex proteoglycan that has a poly-LacNAc chain that can be modified by diverse patterns of sulfate esters at C-6 positions of galactoside (Gal) and N-acetylglucosamine (GlcNAc) residues. Here, a c…
View article: Chemoenzymatic Synthesis of Sulfated <i>N</i>-Glycans Recognized by Siglecs and Other Glycan-Binding Proteins
Chemoenzymatic Synthesis of Sulfated <i>N</i>-Glycans Recognized by Siglecs and Other Glycan-Binding Proteins Open
Sulfated N-glycans are present in many glycoproteins, which are implicated in playing important roles in biological recognition processes. Here, we report the systematic chemoenzymatic synthesis of a library of sulfated and sialylat…
View article: A Biomimetic Synthetic Strategy Can Provide Keratan Sulfate I and II Oligosaccharides with Diverse Fucosylation and Sulfation Patterns
A Biomimetic Synthetic Strategy Can Provide Keratan Sulfate I and II Oligosaccharides with Diverse Fucosylation and Sulfation Patterns Open
Keratan sulfate (KS) is a proteoglycan that is widely expressed in the extracellular matrix of various tissue types, where it performs multiple biological functions. KS is the least understood proteoglycan, which in part is due to a lack o…
View article: A Biomimetic Synthetic Strategy can Provide Keratan Sulfate I and II Oligosaccharides with Diverse Fucosylation and Sulfation Patterns
A Biomimetic Synthetic Strategy can Provide Keratan Sulfate I and II Oligosaccharides with Diverse Fucosylation and Sulfation Patterns Open
Keratan sulfate (KS) is a proteoglycan that is widely expressed in the extracellular matrix of various tissue types where it performs multiple biological functions. KS is the least understood proteoglycan, which in part is due to a lack of…
View article: Glycosyltransferase 8 domain-containing protein 1 (GLT8D1) is a UDP-dependent galactosyltransferase
Glycosyltransferase 8 domain-containing protein 1 (GLT8D1) is a UDP-dependent galactosyltransferase Open
Glycosyltransferases (GTs) are enzymes that catalyze the formation of glycosidic bonds and hundreds of GTs have been identified so far in humans. Glycosyltransferase 8 domain-containing protein 1 (GLT8D1) has been associated with central n…
View article: Exploiting Substrate Specificities of 6- <i>O</i> -Sulfotransferases to Enzymatically Synthesize Keratan Sulfate Oligosaccharides
Exploiting Substrate Specificities of 6- <i>O</i> -Sulfotransferases to Enzymatically Synthesize Keratan Sulfate Oligosaccharides Open
Keratan sulfate (KS) is a glycosaminoglycan that is widely expressed in the extracellular matrix of various tissue types, where it is involved in many biological processes. Herein, we describe a chemo-enzymatic approach to preparing well-d…
View article: Chemoenzymatic Tagging of Tn/TF/STF Antigens in Living Systems
Chemoenzymatic Tagging of Tn/TF/STF Antigens in Living Systems Open
Truncated mucin‐type O‐glycans, such as Tn‐associated antigens, are aberrantly expressed biomarkers of cancer, but remain challenging to target. Reactive antibodies to these antigens either lack high affinity or are prone to antigen escape…
View article: Exploiting Substrate Specificities of 6-<i>O</i>-Sulfotransferases to Enzymatically Synthesize Keratan Sulfate Oligosaccharides
Exploiting Substrate Specificities of 6-<i>O</i>-Sulfotransferases to Enzymatically Synthesize Keratan Sulfate Oligosaccharides Open
Keratan sulfate (KS) is a glycosaminoglycan that is widely expressed in the extracellular matrix of various tissue types where it is involved in many biological processes. Herein, we describe a chemo-enzymatic approach to prepare well-defi…
View article: The Plasminogen-Apple-Nematode (PAN) domain suppresses JA/ET defense pathways in plants
The Plasminogen-Apple-Nematode (PAN) domain suppresses JA/ET defense pathways in plants Open
Suppression of immune response is a phenomenon that enables biological processes such as gamete fertilization, cell growth, cell proliferation, endophyte recruitment, parasitism, and pathogenesis. Here, we show for the first time that the …
View article: Structural basis for Lewis antigen synthesis by the α1,3-fucosyltransferase FUT9
Structural basis for Lewis antigen synthesis by the α1,3-fucosyltransferase FUT9 Open
View article: Bacterial mixed cultures followed mixed-order kinetics during mono-azo dye decolourization and degradation in batch studies
Bacterial mixed cultures followed mixed-order kinetics during mono-azo dye decolourization and degradation in batch studies Open
Over the years little has been focused on kinetic and thermodynamic aspect of bioremediation studies on dyes. In this study, detailed kinetic and thermodynamic analyses of results obtained during a primary study on a mono-azo dye: Reactive…
View article: Structural and biochemical insight into a modular β-1,4-galactan synthase in plants
Structural and biochemical insight into a modular β-1,4-galactan synthase in plants Open
View article: Structural basis for heparan sulfate co-polymerase action by the EXT1–2 complex
Structural basis for heparan sulfate co-polymerase action by the EXT1–2 complex Open
View article: Abstract 1388: Structural basis for heparan sulfate co-polymerase action by the EXT1-2 complex
Abstract 1388: Structural basis for heparan sulfate co-polymerase action by the EXT1-2 complex Open
View article: A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans
A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans Open
View article: Sequential in vitro enzymatic N-glycoprotein modification reveals site-specific rates of glycoenzyme processing
Sequential in vitro enzymatic N-glycoprotein modification reveals site-specific rates of glycoenzyme processing Open
View article: Site-selective sulfation of N-glycans by human GlcNAc-6-O-sulfotransferase 1 (CHST2) and chemoenzymatic synthesis of sulfated antibody glycoforms
Site-selective sulfation of N-glycans by human GlcNAc-6-O-sulfotransferase 1 (CHST2) and chemoenzymatic synthesis of sulfated antibody glycoforms Open
View article: Sequential <i>in vitro</i> enzymatic N-glycoprotein modification reveals site-specific rates of glycoenzyme processing
Sequential <i>in vitro</i> enzymatic N-glycoprotein modification reveals site-specific rates of glycoenzyme processing Open
N-glycosylation is an essential eukaryotic post-translational modification that affects various glycoprotein properties, including folding, solubility, protein-protein interactions, and half-life. N-glycans are processed in the secretory p…
View article: Structural and Biochemical Insight into a Modular β-1,4-Galactan Synthase in Plants
Structural and Biochemical Insight into a Modular β-1,4-Galactan Synthase in Plants Open
Rhamnogalacturonan I (RGI) is a structurally complex pectic polysaccharide with a backbone of alternating rhamnose and galacturonic acid residues substituted with arabinan and galactan side chains. Galactan synthase 1 (GalS1), transfers ga…
View article: Extracellular sialyltransferase st6gal1 in breast tumor cell growth and invasiveness
Extracellular sialyltransferase st6gal1 in breast tumor cell growth and invasiveness Open
The sialyltransferase ST6GAL1 that adds α2–6 linked sialic acids to N-glycans of cell surface and secreted glycoproteins is prominently associated with many human cancers. Tumor-native ST6GAL1 promotes tumor cell behaviors such as invasion…
View article: Extracellular ST6GAL1 regulates monocyte–macrophage development and survival
Extracellular ST6GAL1 regulates monocyte–macrophage development and survival Open
Interaction of immune cells with the systemic environment is necessary for the coordinated development and execution of immune responses. Monocyte-macrophage lineage cells reside at the junction of innate and adaptive immunity. Previously …
View article: Integrated Chemoenzymatic Approach to Streamline the Assembly of Complex Glycopeptides in the Liquid Phase
Integrated Chemoenzymatic Approach to Streamline the Assembly of Complex Glycopeptides in the Liquid Phase Open
Glycosylation of proteins is a complicated post-translational modification. Despite the significant progress in glycoproteomics, accurate functions of glycoproteins are still ambiguous owing to the difficulty in obtaining homogeneous glyco…
View article: Elucidating Human Milk Oligosaccharide biosynthetic genes through network-based multi-omics integration
Elucidating Human Milk Oligosaccharide biosynthetic genes through network-based multi-omics integration Open
View article: Human Intelectin-1 Promotes Cellular Attachment and Neutrophil Killing of Streptococcus pneumoniae in a Serotype-Dependent Manner
Human Intelectin-1 Promotes Cellular Attachment and Neutrophil Killing of Streptococcus pneumoniae in a Serotype-Dependent Manner Open
Human intelectin-1 (hIntL-1) is a secreted glycoprotein capable of binding exocyclic 1,2-diols within surface glycans of human pathogens such as Streptococcus pneumoniae , Vibrio cholerae , and Helicobacter pylori . For the latter, lectin …
View article: Glycan remodeled erythrocytes facilitate antigenic characterization of recent A/H3N2 influenza viruses
Glycan remodeled erythrocytes facilitate antigenic characterization of recent A/H3N2 influenza viruses Open
During circulation in humans and natural selection to escape antibody recognition for decades, A/H3N2 influenza viruses emerged with altered receptor specificities. These viruses lost the ability to agglutinate erythrocytes critical for an…