Dimitrios Latousakis
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View article: A structural basis for the strain-dependent UDP-sugar specificity of glycosyltransferase C from the <i>Limosilactobacillus reuteri</i> accessory secretion system
A structural basis for the strain-dependent UDP-sugar specificity of glycosyltransferase C from the <i>Limosilactobacillus reuteri</i> accessory secretion system Open
The accessory secretion (aSec) system is a protein export pathway that is uniquely present in Gram-positive bacteria and is dedicated to the secretion of large, glycosylated cell wall-anchored adhesins called serine-rich repeat proteins (S…
View article: Proteomics of Bacterial and Mouse Extracellular Vesicles Released in the Gastrointestinal Tracts of Nutrient-Stressed Animals Reveals an Interplay Between Microbial Serine Proteases and Mammalian Serine Protease Inhibitors
Proteomics of Bacterial and Mouse Extracellular Vesicles Released in the Gastrointestinal Tracts of Nutrient-Stressed Animals Reveals an Interplay Between Microbial Serine Proteases and Mammalian Serine Protease Inhibitors Open
Bacterial extracellular vesicles (BEVs) produced by members of the intestinal microbiota can not only contribute to digestion but also mediate microbe–host cell communication via the transfer of functional biomolecules to mammalian host ce…
View article: Proteomics of Bacterial and Mouse Extracellular Vesicles Released in the Gastrointestinal Tract of Nutrient Stressed Animals Reveals an Interplay Between Microbial Serine Proteases and Mammalian Serine Protease Inhibitors
Proteomics of Bacterial and Mouse Extracellular Vesicles Released in the Gastrointestinal Tract of Nutrient Stressed Animals Reveals an Interplay Between Microbial Serine Proteases and Mammalian Serine Protease Inhibitors Open
Bacterial extracellular vesicles (BEVs) produced by members of the intestinal microbiota can contribute not only to digestion but also mediate microbe-host cell communication via the transfer of functional biomolecules to mammalian host ce…
View article: Human milk oligosaccharide 2�-fucosyllactose protects against high-fat diet-induced obesity by changing intestinal mucus production, composition and degradation linked to changes in gut microbiota and faecal proteome profiles in mice
Human milk oligosaccharide 2�-fucosyllactose protects against high-fat diet-induced obesity by changing intestinal mucus production, composition and degradation linked to changes in gut microbiota and faecal proteome profiles in mice Open
Objective To decipher the mechanisms by which the major human milk oligosaccharide (HMO), 2�-fucosyllactose (2�FL), can affect body weight and fat mass gain on high-fat diet (HFD) feeding in mice. We wanted to elucidate whether 2�FL metabo…
View article: Role of mucin glycosylation in the gut microbiota-brain axis of core 3 O-glycan deficient mice
Role of mucin glycosylation in the gut microbiota-brain axis of core 3 O-glycan deficient mice Open
Alterations in intestinal mucin glycosylation have been associated with increased intestinal permeability and sensitivity to inflammation and infection. Here, we used mice lacking core 3-derived O -glycans (C3GnT −/− ) to investigate the e…
View article: Correction: Biochemical and structural basis of sialic acid utilization by gut microbes
Correction: Biochemical and structural basis of sialic acid utilization by gut microbes Open
In the JBC Reviews article entitled "Biochemical and structural basis of sialic acid utilization by gut microbes" arrows on Figure 6 should be adjusted.The correct Figure 6 is provided below.
View article: Biochemical and structural basis of sialic acid utilization by gut microbes
Biochemical and structural basis of sialic acid utilization by gut microbes Open
View article: The role of the mucin-glycan foraging <i>Ruminococcus gnavus</i> in the communication between the gut and the brain
The role of the mucin-glycan foraging <i>Ruminococcus gnavus</i> in the communication between the gut and the brain Open
Ruminococcus gnavus is a prevalent member of the human gut microbiota, which is over-represented in inflammatory bowel disease and neurological disorders. We previously showed that the ability of R. gnavus to forage on mucins…
View article: Biochemical Basis of Xylooligosaccharide Utilisation by Gut Bacteria
Biochemical Basis of Xylooligosaccharide Utilisation by Gut Bacteria Open
Xylan is one of the major structural components of the plant cell wall. Xylan present in the human diet reaches the large intestine undigested and becomes a substrate to species of the gut microbiota. Here, we characterised the capacity of…
View article: The human gut symbiont Ruminococcus gnavus shows specificity to blood group A antigen during mucin glycan foraging: Implication for niche colonisation in the gastrointestinal tract
The human gut symbiont Ruminococcus gnavus shows specificity to blood group A antigen during mucin glycan foraging: Implication for niche colonisation in the gastrointestinal tract Open
The human gut symbiont Ruminococcus gnavus displays strain-specific repertoires of glycoside hydrolases (GHs) contributing to its spatial location in the gut. Sequence similarity network analysis identified strain-specific differences in b…
View article: Lipopolysaccharide associated with β-2,6 fructan mediates TLR4-dependent immunomodulatory activity in vitro
Lipopolysaccharide associated with β-2,6 fructan mediates TLR4-dependent immunomodulatory activity in vitro Open
View article: The Immunomodulatory Properties of β-2,6 Fructans: A Comprehensive Review
The Immunomodulatory Properties of β-2,6 Fructans: A Comprehensive Review Open
Polysaccharides such as β-2,1-linked fructans including inulin or fructose oligosaccharides are well-known prebiotics with recognised immunomodulatory properties. In recent years, other fructan types covering β-2,6-linked fructans, particu…
View article: Uncovering a novel molecular mechanism for scavenging sialic acids in bacteria
Uncovering a novel molecular mechanism for scavenging sialic acids in bacteria Open
View article: Elucidation of a sialic acid metabolism pathway in mucus-foraging Ruminococcus gnavus unravels mechanisms of bacterial adaptation to the gut
Elucidation of a sialic acid metabolism pathway in mucus-foraging Ruminococcus gnavus unravels mechanisms of bacterial adaptation to the gut Open
View article: Serine-rich repeat proteins from gut microbes
Serine-rich repeat proteins from gut microbes Open
Serine-rich repeat proteins (SRRPs) have emerged as an important group of cell surface adhesins found in a growing number of Gram-positive bacteria. Studies focused on SRRPs from streptococci and staphylococci demonstrated that these prote…
View article: Serine-rich repeat protein adhesins from<i>Lactobacillus reuteri</i>display strain specific glycosylation profiles
Serine-rich repeat protein adhesins from<i>Lactobacillus reuteri</i>display strain specific glycosylation profiles Open
Lactobacillus reuteri is a gut symbiont inhabiting the gastrointestinal tract of numerous vertebrates. The surface-exposed serine-rich repeat protein (SRRP) is a major adhesin in Gram-positive bacteria. Using lectin and sugar nucleotide pr…
View article: Structural basis for the role of serine-rich repeat proteins from <i>Lactobacillus reuteri</i> in gut microbe–host interactions
Structural basis for the role of serine-rich repeat proteins from <i>Lactobacillus reuteri</i> in gut microbe–host interactions Open
Significance Gut bacteria play a key role in health and disease, but the molecular mechanisms underpinning their interaction with the host remain elusive. The serine-rich repeat proteins (SRRPs) are a family of adhesins identified in many …
View article: Molecular basis for intestinal mucin recognition by galectin‐3 and C‐type lectins
Molecular basis for intestinal mucin recognition by galectin‐3 and C‐type lectins Open
Intestinal mucins trigger immune responses upon recognition by dendritic cells via protein–carbohydrate interactions. We used a combination of structural, biochemical, biophysical, and cell‐based approaches to decipher the specificity of t…
View article: How Sweet Are Our Gut Beneficial Bacteria? A Focus on Protein Glycosylation in Lactobacillus
How Sweet Are Our Gut Beneficial Bacteria? A Focus on Protein Glycosylation in Lactobacillus Open
Protein glycosylation is emerging as an important feature in bacteria. Protein glycosylation systems have been reported and studied in many pathogenic bacteria, revealing an important diversity of glycan structures and pathways within and …
View article: How sweet are our gut beneficial microbes: Protein glycosylation in Lactobacillus reuteri
How sweet are our gut beneficial microbes: Protein glycosylation in Lactobacillus reuteri Open
Protein glycosylation is a well-established post translational modification occurring in all forms of life. In the past two decades, protein glycosylation has been extensively studied in bacterial pathogens underscoring its importance in v…
View article: Membrane-enclosed multienzyme (MEME) synthesis of 2,7-anhydro-sialic acid derivatives
Membrane-enclosed multienzyme (MEME) synthesis of 2,7-anhydro-sialic acid derivatives Open
Naturally occurring 2,7-anhydro-alpha-N-acetylneuraminic acid (2,7-anhydro-Neu5Ac) is a transglycosylation product of bacterial intramolecular trans-sialidases (IT-sialidases). A facile one-pot two-enzyme approach has been established for …
View article: Structural Dissection of the Maltodextrin Disproportionation Cycle of the Arabidopsis Plastidial Disproportionating Enzyme 1 (DPE1)
Structural Dissection of the Maltodextrin Disproportionation Cycle of the Arabidopsis Plastidial Disproportionating Enzyme 1 (DPE1) Open