Dirk Bumann
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View article: Microgeography of staphyloccoci in human tissue explains antibiotic failure
Microgeography of staphyloccoci in human tissue explains antibiotic failure Open
Summary paragraph Bacterial infections remain a major health threat, yet pathogen biology in human tissues is poorly understood. Using AI-guided imaging, we mapped ∼15,500 Staphylococcus aureus cells in biopsies from 33 patients undergoing…
View article: Tackling microbial iron homeostasis: novel antimicrobial strategies
Tackling microbial iron homeostasis: novel antimicrobial strategies Open
The escalating threat of antimicrobial resistance demands innovative therapeutic strategies beyond classical targets. Recent insights into the mechanisms of bacterial iron acquisition - ranging from siderophores and heme uptake to ferrous …
View article: Unveiling signaling pathways inducing MHC class II expression in neutrophils
Unveiling signaling pathways inducing MHC class II expression in neutrophils Open
Introduction Gram-negative bacillary bacteremia poses a significant threat, ranking among the most severe infectious diseases capable of triggering life-threatening sepsis. Despite the unambiguous involvement of neutrophils in this potenti…
View article: A Far-Red Fluorescent Probe to Visualize Gram-Positive Bacteria in Patient Samples
A Far-Red Fluorescent Probe to Visualize Gram-Positive Bacteria in Patient Samples Open
Gram-positive bacteria, in particular Staphylococcus aureus (S. aureus), are the leading bacterial cause of death in high-income countries and can cause invasive infections at various body sites. These infections are associated with prolon…
View article: Ectopical expression of bacterial collagen-like protein supports its role as adhesin in host–parasite coevolution
Ectopical expression of bacterial collagen-like protein supports its role as adhesin in host–parasite coevolution Open
For a profound understanding of antagonistic coevolution, it is necessary to identify the coevolving genes. The bacterium Pasteuria and its host, the microcrustacean Daphnia , are a well-characterized paradigm for co-evolution, but the und…
View article: Heterogeneous dual-metal control of<i>Salmonella</i>infection
Heterogeneous dual-metal control of<i>Salmonella</i>infection Open
Iron controls bacterial infections through diverse pathogen and host mechanisms that remain challenging to disentangle. Here, we determined how individual Salmonella cells access iron in infected mice. Our results showed that the iron tran…
View article: A Far-Red Fluorescent Probe to Visualize<i>Staphylococcus aureus</i>in Patient Samples
A Far-Red Fluorescent Probe to Visualize<i>Staphylococcus aureus</i>in Patient Samples Open
Staphylococcus aureus (S. aureus) is the leading bacterial cause of death in high-income countries and can cause invasive infections at various body sites. These infections are associated with prolonged hospital stays, a large economic bur…
View article: Ectopical expression of bacterial collagen-like protein supports its role as adhesin in host-parasite coevolution
Ectopical expression of bacterial collagen-like protein supports its role as adhesin in host-parasite coevolution Open
For a profound understanding of the mechanisms of antagonistic coevolution, it is necessary to identify the coevolving genes. The spore-forming bacterium Pasteuria ramosa and its host, the microcrustacean Daphnia , are a well-characterized…
View article: Zebrafish Larvae as an in vivo Model for Antimicrobial Activity Tests against Intracellular Salmonella
Zebrafish Larvae as an in vivo Model for Antimicrobial Activity Tests against Intracellular Salmonella Open
Introduction: Blood infections from multi-drug-resistant Salmonella pose a major health burden. This is especially true because Salmonella can survive and replicate intracellularly, and the development of new treatment strategies is depend…
View article: Importance of aspartyl protease 5 in the establishment of the intracellular niche during acute and chronic infection of <i>Toxoplasma gondii</i>
Importance of aspartyl protease 5 in the establishment of the intracellular niche during acute and chronic infection of <i>Toxoplasma gondii</i> Open
Virulence and persistence of the obligate intracellular parasite Toxoplasma gondii involve the secretion of effector proteins belonging to the family of dense granule proteins (GRAs) that act notably as modulators of the host defense mecha…
View article: Plant-Derived Catechols Are Substrates of TonB-Dependent Transporters and Sensitize Pseudomonas aeruginosa to Siderophore-Drug Conjugates
Plant-Derived Catechols Are Substrates of TonB-Dependent Transporters and Sensitize Pseudomonas aeruginosa to Siderophore-Drug Conjugates Open
Iron is an essential element for living organisms. Most bacteria synthesize species-specific iron chelators, called siderophores, able to capture iron from their host or the environment. Pseudomonas aeruginosa , an opportunistic pathogen, …
View article: Plant-derived catechols are substrates of TonB-dependent transporters and sensitize Pseudomonas aeruginosa to siderophore-drug conjugates
Plant-derived catechols are substrates of TonB-dependent transporters and sensitize Pseudomonas aeruginosa to siderophore-drug conjugates Open
The dataset contains raw data used to generate the figures of the associated mansucript
View article: Plant-derived catechols are substrates of TonB-dependent transporters and sensitize Pseudomonas aeruginosa to siderophore-drug conjugates
Plant-derived catechols are substrates of TonB-dependent transporters and sensitize Pseudomonas aeruginosa to siderophore-drug conjugates Open
The dataset contains raw data used to generate the figures of the associated mansucript
View article: Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate?
Making Antimicrobial Susceptibility Testing More Physiologically Relevant with Bicarbonate? Open
Azithromycin is a clinically important drug for treating invasive salmonellosis despite poor activity in laboratory assays for MIC. Addition of the main buffer in blood, bicarbonate, has been proposed for more physiologically relevant and …
View article: Tissue compartmentalization enables<i>Salmonella</i>persistence during chemotherapy
Tissue compartmentalization enables<i>Salmonella</i>persistence during chemotherapy Open
Significance Some infections are exceedingly difficult to cure, even with adequate antimicrobial chemotherapy. How pathogens can survive exposure to antimicrobials in tissues remains poorly understood. Using three-dimensional whole-organ t…
View article: Acquisition of ionic copper by the bacterial outer membrane protein OprC through a novel binding site
Acquisition of ionic copper by the bacterial outer membrane protein OprC through a novel binding site Open
Copper, while toxic in excess, is an essential micronutrient in all kingdoms of life due to its essential role in the structure and function of many proteins. Proteins mediating ionic copper import have been characterised in detail for euk…
View article: Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in <i>Pseudomonas aeruginosa</i>
Outer membrane permeability: Antimicrobials and diverse nutrients bypass porins in <i>Pseudomonas aeruginosa</i> Open
Significance Novel antibiotics are urgently needed to resolve the current antimicrobial resistance crisis. For critical pathogens, drug entry through the cell envelope is one of the major challenges in the development of effective novel an…
View article: Ferritin H deficiency deteriorates cellular iron handling and worsens Salmonella typhimurium infection by triggering hyperinflammation
Ferritin H deficiency deteriorates cellular iron handling and worsens Salmonella typhimurium infection by triggering hyperinflammation Open
Iron is an essential nutrient for mammals as well as for pathogens. Inflammation-driven changes in systemic and cellular iron homeostasis are central for host-mediated antimicrobial strategies. Here, we studied the role of the iron storage…
View article: Increased Production of Outer Membrane Vesicles by Salmonella Interferes with Complement-Mediated Innate Immune Attack
Increased Production of Outer Membrane Vesicles by Salmonella Interferes with Complement-Mediated Innate Immune Attack Open
Bacterial outer membrane vesicles (OMVs) mediate critical bacterium-bacterium and host-microbial interactions that influence pathogenesis through multiple mechanisms, including the elicitation of inflammatory responses, delivery of virulen…
View article: Molecular reprogramming and phenotype switching in <i>Staphylococcus aureus</i> lead to high antibiotic persistence and affect therapy success
Molecular reprogramming and phenotype switching in <i>Staphylococcus aureus</i> lead to high antibiotic persistence and affect therapy success Open
Significance Persisters represent a bacterial subpopulation that survive high antibiotic concentrations without being resistant. Their role in clinics in persistent infections and their molecular and functional landscape is not fully estab…
View article: The pathogen<i>Pseudomonas aeruginosa</i>optimizes the production of the siderophore pyochelin upon environmental challenges
The pathogen<i>Pseudomonas aeruginosa</i>optimizes the production of the siderophore pyochelin upon environmental challenges Open
Siderophores are iron chelators produced by bacteria to access iron, an essential nutrient. The pathogen Pseudomonas aeruginosa produces two siderophores, pyoverdine and pyochelin, the former with a high affinity for iron and the latter wi…
View article: Regulation of chaperone function by coupled folding and oligomerization
Regulation of chaperone function by coupled folding and oligomerization Open
Trimeric chaperone Skp activates by an unprecedented mechanism, with folding from a disordered state coupled to trimerization.
View article: Single-cell reporters for pathogen responses to antimicrobial host attacks
Single-cell reporters for pathogen responses to antimicrobial host attacks Open
Host-pathogen interactions are often heterogeneous involving individual encounters between host and pathogen cells with diverse molecular mechanisms, response networks, and diverging outcomes. Single-cell reporters can identify the various…
View article: Acquisition of ionic copper by a bacterial outer membrane protein
Acquisition of ionic copper by a bacterial outer membrane protein Open
Copper, while toxic in excess, is an essential micronutrient in all kingdoms of life due to its essential role in the structure and function of many proteins. Proteins mediating ionic copper import have been characterised in detail for euk…
View article: Efficient Dual-Negative Selection for Bacterial Genome Editing
Efficient Dual-Negative Selection for Bacterial Genome Editing Open
We describe a versatile method for chromosomal gene editing based on classical consecutive single-crossovers. The method exploits rapid plasmid construction using Gibson assembly, a convenient E. coli donor strain, and efficient dual-negat…