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View article: Boosted Pro-Inflammatory Activity in Human PBMCs by Lipopolysaccharide and SARS-CoV-2 Spike Protein Is Regulated by α-1 Antitrypsin
Boosted Pro-Inflammatory Activity in Human PBMCs by Lipopolysaccharide and SARS-CoV-2 Spike Protein Is Regulated by α-1 Antitrypsin Open
For the treatment of severe COVID-19, supplementation with human plasma-purified α-1 antitrypsin (AAT) to patients is currently considered. AAT inhibits host proteases that facilitate viral entry and possesses broad anti-inflammatory and i…
View article: Trimeric SARS-CoV-2 Spike Proteins Produced from CHO Cells in Bioreactors Are High-Quality Antigens
Trimeric SARS-CoV-2 Spike Proteins Produced from CHO Cells in Bioreactors Are High-Quality Antigens Open
The spike protein of the pandemic human corona virus is essential for its entry into human cells. In fact, most neutralizing antibodies against Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) are directed against the Virus-su…
View article: Trimeric SARS-CoV-2 Spike proteins produced from CHO-cells in bioreactors are high quality antigens
Trimeric SARS-CoV-2 Spike proteins produced from CHO-cells in bioreactors are high quality antigens Open
The Spike protein of SARS-CoV-2 is essential for virus entry into human cells. In fact, most neutralizing antibodies against SARS-CoV-2 are directed against the Spike, making it the antigen of choice for use in vaccines and diagnostic test…
View article: Designs and Characterization of Subunit Ebola GP Vaccine Candidates: Implications for Immunogenicity
Designs and Characterization of Subunit Ebola GP Vaccine Candidates: Implications for Immunogenicity Open
The humoral responses of Ebola virus (EBOV) survivors mainly target the surface glycoprotein GP, and anti-GP neutralizing antibodies have been associated with protection against EBOV infection. In order to elicit protective neutralizing an…
View article: Enhanced plasmid DNA utilization in transiently transfected CHO–DG44 cells in the presence of polar solvents
Enhanced plasmid DNA utilization in transiently transfected CHO–DG44 cells in the presence of polar solvents Open
Although the protein yields from transient gene expression (TGE) with Chinese hamster ovary (CHO) cells have recently improved, the amount of plasmid DNA (pDNA) needed for transfection remains relatively high. We describe a strategy to red…
View article: Transcriptional and post‐transcriptional limitations of high‐yielding, <scp>PEI</scp>‐mediated transient transfection with <scp>CHO</scp> and <scp>HEK‐293E</scp> cells
Transcriptional and post‐transcriptional limitations of high‐yielding, <span>PEI</span>‐mediated transient transfection with <span>CHO</span> and <span>HEK‐293E</span> cells Open
Transient gene expression (TGE) in human embryonic kidney (HEK‐293) and Chinese hamster ovary (CHO) cells is a well‐established technology for the rapid generation of recombinant proteins. Although the maximum TGE yields have reached 1 g/L…