Dmitriy V. Sotnikov
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View article: Enhanced Lateral Flow Immunoassay for Pesticide Paraquat Based on Combining Magnetite and Gold Nanoparticles
Enhanced Lateral Flow Immunoassay for Pesticide Paraquat Based on Combining Magnetite and Gold Nanoparticles Open
A lateral flow immunoassay (LFIA) utilizing two types of marker conjugates—magnetite nanoparticles (MNPs) with specific antibodies and gold nanoparticles (GNPs) with anti-species antibodies—was proposed and realized for the detection of th…
View article: Enhanced Emission of Fluorescein Label in Immune Complexes Provides for Rapid Homogeneous Assay of Aflatoxin B1
Enhanced Emission of Fluorescein Label in Immune Complexes Provides for Rapid Homogeneous Assay of Aflatoxin B1 Open
Homogeneous fluorescence immunoassays are in high demand due to their simplicity, rapidity, sensitivity, and specificity. These methods typically utilize immune-induced changes in the rotational mobility of the fluorophore with depolarizat…
View article: Advancing Lateral Flow Detection in CRISPR/Cas12a Systems Through Rational Understanding and Design Strategies of Reporter Interactions
Advancing Lateral Flow Detection in CRISPR/Cas12a Systems Through Rational Understanding and Design Strategies of Reporter Interactions Open
CRISPR/Cas12a systems coupled with lateral flow tests (LFTs) are a promising route to rapid, instrument-free nucleic acid diagnostics due to conversion target recognition into a simple visual readout via cleavage of dual-labeled single-str…
View article: Comparative Characteristics of Immunochromatographic Test Systems for Tylosin Antibiotic in Meat Products
Comparative Characteristics of Immunochromatographic Test Systems for Tylosin Antibiotic in Meat Products Open
Tylosin (TYL) is a macrolide antibiotic widely used in animal husbandry. Due to associated health risks, there is a demand for sensitive methods for mass screening of TYL in products of animal origin. This article describes the development…
View article: Automation of adaptive control of complex objects states trajectories in artificial intelligence systems
Automation of adaptive control of complex objects states trajectories in artificial intelligence systems Open
Today, the problem of automating the control of the individual trajectory of the states of complex objects is relevant. It is necessary to influence the individual trajectory of the object's states, guided by certain parameters. The reacti…
View article: Ability of Antibodies Immobilized on Gold Nanoparticles to Bind Small Antigen Fluorescein
Ability of Antibodies Immobilized on Gold Nanoparticles to Bind Small Antigen Fluorescein Open
The analytical applications of antibodies are often associated with their immobilization on different carriers, which is accompanied by a loss of antigen-binding activity for a sufficient proportion of the bound antibodies. In contrast to …
View article: Comparison of Three Lateral Flow Immunoassay Formats for the Detection of Antibodies against the SARS-CoV-2 Antigen
Comparison of Three Lateral Flow Immunoassay Formats for the Detection of Antibodies against the SARS-CoV-2 Antigen Open
Reliable detection of specific antibodies against pathogens by lateral flow immunoassay (LFIA) greatly depends on the composition of the detectable complex and the order of its assembly. We compared three LFIA formats for revealing anti-SA…
View article: A Dimension Selection-Based Constrained Multi-Objective Optimization Algorithm Using a Combination of Artificial Intelligence Methods
A Dimension Selection-Based Constrained Multi-Objective Optimization Algorithm Using a Combination of Artificial Intelligence Methods Open
The computational cost of modern simulation-based optimization tends to be prohibitive in practice. Complex design problems often involve expensive constraints evaluated through finite element analysis or other computationally intensive pr…
View article: Enhanced Lateral Flow Immunoassay with Double Competition and Two Kinds of Nanoparticles Conjugates for Control of Insecticide Imidacloprid in Honey
Enhanced Lateral Flow Immunoassay with Double Competition and Two Kinds of Nanoparticles Conjugates for Control of Insecticide Imidacloprid in Honey Open
Finding optimal conditions for competitive lateral flow immunoassay is a controversial task. The content of specific antibodies labeled by nanoparticles should be simultaneously high to reach intense signals and low to register an influenc…
View article: Modular Set of Reagents in Lateral Flow Immunoassay: Application for Antibiotic Neomycin Detection in Honey
Modular Set of Reagents in Lateral Flow Immunoassay: Application for Antibiotic Neomycin Detection in Honey Open
A scheme of modular competitive immunochromatography with an analyte-independent test strip and changeable specific immunoreactants has been proposed. Native (detected) and biotinylated antigens interact with specific antibodies during the…
View article: SMART: A Swing-Assisted Multiplexed Analyzer for Point-of-Care Respiratory Tract Infection Testing
SMART: A Swing-Assisted Multiplexed Analyzer for Point-of-Care Respiratory Tract Infection Testing Open
Respiratory tract infections such as the ongoing coronavirus disease 2019 (COVID-19) has seriously threatened public health in the last decades. The experience of fighting against the epidemic highlights the importance of user-friendly and…
View article: Handling Detection Limits of Multiplex Lateral Flow Immunoassay by Choosing the Order of Binding Zones
Handling Detection Limits of Multiplex Lateral Flow Immunoassay by Choosing the Order of Binding Zones Open
Changes in the limits of detection (LODs) for a multiplex lateral flow immunoassay (LFIA) caused by different locations of the binding zone on the test strips were studied. Due to the non-equilibrium conditions of the immune reactions in L…
View article: Silent Antibodies Start Talking: Enhanced Lateral Flow Serodiagnosis with Two-Stage Incorporation of Labels into Immune Complexes
Silent Antibodies Start Talking: Enhanced Lateral Flow Serodiagnosis with Two-Stage Incorporation of Labels into Immune Complexes Open
The presence of pathogen-specific antibodies in the blood is widely controlled by a serodiagnostic technique based on the lateral flow immunoassay (LFIA). However, its common one-stage format with an antigen immobilized in the binding zone…
View article: Double Competitive Immunodetection of Small Analyte: Realization for Highly Sensitive Lateral Flow Immunoassay of Chloramphenicol
Double Competitive Immunodetection of Small Analyte: Realization for Highly Sensitive Lateral Flow Immunoassay of Chloramphenicol Open
A new scheme of reagents interaction for lateral flow immunoassay (LFIA) is proposed, which combines the features of competitive and sandwich assay and provides highly sensitive detection of low-molecular-weight analytes. Namely, the antig…
View article: Silent Antibodies Start Talking: Enhanced Lateral Flow Serodiagnosis with Two-Stage Incorporation of Labels into Immune Complexes
Silent Antibodies Start Talking: Enhanced Lateral Flow Serodiagnosis with Two-Stage Incorporation of Labels into Immune Complexes Open
Nowadays, the presence of pathogen-specific antibodies in the blood is widely controlled by a serodiagnostic technique based on the lateral flow immunoassay (LFIA). However, its common one-stage format with an antigen immobilized in the bi…
View article: Raman Scattering-Based Biosensing: New Prospects and Opportunities
Raman Scattering-Based Biosensing: New Prospects and Opportunities Open
The growing interest in the development of new platforms for the application of Raman spectroscopy techniques in biosensor technologies is driven by the potential of these techniques in identifying chemical compounds, as well as structural…
View article: Retention of Activity by Antibodies Immobilized on Gold Nanoparticles of Different Sizes: Fluorometric Method of Determination and Comparative Evaluation
Retention of Activity by Antibodies Immobilized on Gold Nanoparticles of Different Sizes: Fluorometric Method of Determination and Comparative Evaluation Open
Antibody–nanoparticle conjugates are widely used analytical reagents. An informative parameter reflecting the conjugates’ properties is the number of antibodies per nanoparticle that retain their antigen-binding ability. Estimation of this…
View article: Changing Cross-Reactivity for Different Immunoassays Using the Same Antibodies: Theoretical Description and Experimental Confirmation
Changing Cross-Reactivity for Different Immunoassays Using the Same Antibodies: Theoretical Description and Experimental Confirmation Open
Many applications of immunoassays involve the possible presence of structurally similar compounds that bind with antibodies, but with different affinities. In this regard, an important characteristic of an immunoassay is its cross-reactivi…
View article: First Russian 220 kV Superconducting Fault Current Limiter (SFCL) For Application in City Grid
First Russian 220 kV Superconducting Fault Current Limiter (SFCL) For Application in City Grid Open
The superconducting fault current limiter (SFCL) for a nominal voltage of 220 kV and a rated current of 1200 A was developed by SuperOx company to be applied in high voltage substation in Moscow, Russia. The device is a three-phase dead-ta…
View article: Manufacturing lateral flow tests for tuberculosis diagnosis: choosing a reactants completion and sensing regime
Manufacturing lateral flow tests for tuberculosis diagnosis: choosing a reactants completion and sensing regime Open
To develop technologies for rapid out-of-laboratory diagnoses of tuberculosis based on the level of specific antibodies, the requirements for the antibody preparations and their combinations used in the analysis were studied, as well as pr…
View article: Lateral Flow Serodiagnosis in the Double-Antigen Sandwich Format: Theoretical Consideration and Confirmation of Advantages
Lateral Flow Serodiagnosis in the Double-Antigen Sandwich Format: Theoretical Consideration and Confirmation of Advantages Open
Determination of the presence in the blood of antibodies specific to the causative agent of a particular disease (serodiagnosis) is an effective approach in medical analytical chemistry. Serodiagnostics performed in the lateral flow immuno…
View article: Molecular basis for the recognition of steroidogenic acute regulatory protein by the 14‐3‐3 protein family
Molecular basis for the recognition of steroidogenic acute regulatory protein by the 14‐3‐3 protein family Open
Steroidogenesis in adrenals and gonads starts from cholesterol transport to mitochondria. This is mediated by the steroidogenic acute regulatory protein (STARD1), containing a mitochondrial import sequence followed by a cholesterol‐binding…
View article: Molecular basis for the recognition of steroidogenic acute regulatory protein by the 14-3-3 protein family
Molecular basis for the recognition of steroidogenic acute regulatory protein by the 14-3-3 protein family Open
Steroidogenesis in adrenals and gonads starts from cholesterol transport to mitochondria by the steroidogenic acute regulatory protein STARD1, containing a mitochondrial import sequence followed by a cholesterol-binding START domain. Altho…
View article: DYNAMICS OF HEIGHT RANGE DISTRIBUTION OF TREES IN THE FOREST SHELTER BELT WITH PARTICIPATION OF ENGLISH OAK (QUERCUS ROBUR L.) AND NORWAY MAPLE (ACER PLATANOIDES L.)
DYNAMICS OF HEIGHT RANGE DISTRIBUTION OF TREES IN THE FOREST SHELTER BELT WITH PARTICIPATION OF ENGLISH OAK (QUERCUS ROBUR L.) AND NORWAY MAPLE (ACER PLATANOIDES L.) Open
Protective afforestation is organizing basis of adaptive landscape agriculture. The aim of the study is to distribute the trees of English oak (Quercus robur L.) and Norway maple (Acer platanoides L.) in height to establish the complexity …
View article: Design of Multiplex Lateral Flow Tests: A Case Study for Simultaneous Detection of Three Antibiotics
Design of Multiplex Lateral Flow Tests: A Case Study for Simultaneous Detection of Three Antibiotics Open
The presented study is focused on the impact of binding zone location on immunochromatographic test strips on the analytical parameters of multiplex lateral flow assays. Due to non-equilibrium conditions for such assays the duration of imm…
View article: Correlation between composition and activity of gold nanoparticle conjugates with streptococcal protein G
Correlation between composition and activity of gold nanoparticle conjugates with streptococcal protein G Open
The composition of the conjugates of gold nanoparticles with streptococcal protein G was studied using fluorescence spectroscopy. The method for determining the composition is based on measuring the intrinsic fluorescence of tryptophan as …
View article: Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots
Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots Open
In this article, we describe an immunochromatographic test system developed for rapid serodiagnostics of cattle brucellosis using two markers: Gold nanoparticles (GNPs) and quantum dots (QDs). The test system was compared with immunochroma…
View article: Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots
Immunochromatographic System for Serodiagnostics of Cattle Brucellosis Using Gold Nanoparticles and Signal Amplification with Quantum Dots Open
In this article, we describe an immunochromatographic test system developed for rapid serodiagnostics of cattle brucellosis using two markers: Gold nanoparticles (GNPs) and quantum dots (QDs). The test system was compared with immunochroma…
View article: A Mechanism of Gold Nanoparticle Aggregation by Immunoglobulin G Preparation
A Mechanism of Gold Nanoparticle Aggregation by Immunoglobulin G Preparation Open
Conjugates of gold nanoparticles (GNPs) and antibodies are widely used in various fields of biochemistry and microbiology. However, the procedure for obtaining such conjugates remains precarious, and the properties of conjugates differ sig…
View article: THE COMPOSITION OF GOLD NANOPARTICLE CONJUGATES WITH IMMUNOGLOBULINS AT PHYSICAL ADSORPTION OR COVALENT BINDING
THE COMPOSITION OF GOLD NANOPARTICLE CONJUGATES WITH IMMUNOGLOBULINS AT PHYSICAL ADSORPTION OR COVALENT BINDING Open
Share on Facebook Share on Twitter Send by email THE COMPOSITION OF GOLD NANOPARTICLE CONJUGATES WITH IMMUNOGLOBULINS AT PHYSICAL ADSORPTION OR COVALENT BINDING 1 SOTNIKOV Dmitriy V. Co-authors: 1 BYZOVA Nadezhda A. 1 ZHERDEV Anatoly V. 1 …