Dmitry Torchinsky
YOU?
Author Swipe
View article: Simultaneous global labeling (SiGL) of 5-methylcytosine and 5-hydroxymethylcytosine by DNA alkylation with a synthetic cofactor and engineered methyltransferase
Simultaneous global labeling (SiGL) of 5-methylcytosine and 5-hydroxymethylcytosine by DNA alkylation with a synthetic cofactor and engineered methyltransferase Open
5-methylcytosine and 5-hydroxymethylcytosine are epigenetic modifications involved in gene regulation and cancer. Here, we describe a new, simple, and high-throughput platform for multi-colour epigenetic analysis. The novelty of our approa…
View article: Chemoenzymatic labeling of DNA methylation patterns for single-molecule epigenetic mapping
Chemoenzymatic labeling of DNA methylation patterns for single-molecule epigenetic mapping Open
DNA methylation, specifically, methylation of cytosine (C) nucleotides at the 5-carbon position (5-mC), is the most studied and significant epigenetic modification. Here we developed a chemoenzymatic procedure to fluorescently label non-me…
View article: Chemoenzymatic labeling of DNA methylation patterns for single-molecule epigenetic mapping
Chemoenzymatic labeling of DNA methylation patterns for single-molecule epigenetic mapping Open
DNA methylation, specifically, methylation of cytosine (C) nucleotides at the 5-carbon position (5-mC), is the most studied and among the most significant epigenetic modifications. Here we developed a chemoenzymatic procedure to fluorescen…
View article: Multi-modal Single-molecule Imaging with Continuously Controlled Spectral-resolution (CoCoS) Microscopy
Multi-modal Single-molecule Imaging with Continuously Controlled Spectral-resolution (CoCoS) Microscopy Open
Color is a fundamental contrast mechanism in fluorescence microscopy, providing the basis for numerous imaging and spectroscopy techniques. The ever-growing need to acquire high-throughput, dynamic data from multicolor species is driving t…
View article: Rapid Quantification of Oxidation and UV Induced DNA Damage by Repair Assisted Damage Detection-(Rapid RADD)
Rapid Quantification of Oxidation and UV Induced DNA Damage by Repair Assisted Damage Detection-(Rapid RADD) Open
Knowing the amount and type of DNA damage is of great significance for a broad range of clinical and research applications. However, existing methods are either lacking in their ability to distinguish between types of DNA damage or limited…
View article: Label as you fold: methyltransferase-assisted functionalization of DNA nanostructures
Label as you fold: methyltransferase-assisted functionalization of DNA nanostructures Open
The DNA methyltransferase M.TaqI is presented as a new tool to label DNA nanostructures covalently with fluorophores and azide groups.
View article: Global modulation in DNA epigenetics during pro-inflammatory macrophage activation
Global modulation in DNA epigenetics during pro-inflammatory macrophage activation Open
DNA methylation patterns create distinct gene-expression profiles. These patterns are maintained after cell division, thus enabling the differentiation and maintenance of multiple cell types from the same genome sequence. The advantage of …
View article: Global Modulation in DNA Epigenetics during Pro-Inflammatory Macrophage Activation
Global Modulation in DNA Epigenetics during Pro-Inflammatory Macrophage Activation Open
DNA methylation patterns create distinct gene expression profiles. These patterns are maintained after cell division, thus enabling the differentiation and maintenance of multiple cell types from the same genome sequence. The advantage of …
View article: Long-read single-molecule maps of the functional methylome
Long-read single-molecule maps of the functional methylome Open
We report on the development of a methylation analysis workflow for optical detection of fluorescent methylation profiles along chromosomal DNA molecules. In combination with Bionano Genomics genome mapping technology, these profiles provi…
View article: Global modulation in DNA epigenetics during pro-inflammatory macrophage activation
Global modulation in DNA epigenetics during pro-inflammatory macrophage activation Open
DNA methylation patterns create distinct gene-expression profiles. These patterns are maintained after cell division, thus enabling the differentiation and maintenance of multiple cell types from the same genome sequence. The advantage of …
View article: Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling
Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling Open
Specific and simultaneous quantitation of DNA adducts is a major obstacle. Using repair enzymes, we present a protocol to quantify two types of DNA lesions simultaneously on the same DNA molecule and examine repair dynamics by single-molec…
View article: Global Modulation in DNA Epigenetics during Pro-Inflammatory Macrophage Activation
Global Modulation in DNA Epigenetics during Pro-Inflammatory Macrophage Activation Open
DNA methylation patterns create distinct gene expression profiles. These patterns are maintained after cell division, thus enabling the differentiation and maintenance of multiple cell types from the same genome sequence. The advantage of …
View article: Microfluidic DNA combing for parallel single-molecule analysis
Microfluidic DNA combing for parallel single-molecule analysis Open
DNA combing is a widely used method for stretching and immobilising DNA molecules on a surface. Fluorescent labelling of genomic information enables high-resolution optical analysis of DNA at the single-molecule level. Despite its simplici…
View article: Reduced representation optical methylation mapping (R<sup>2</sup>OM<sup>2</sup>)
Reduced representation optical methylation mapping (R<sup>2</sup>OM<sup>2</sup>) Open
Reduced representation methylation profiling is a method of analysis in which a subset of CpGs is used to report the overall methylation status of the probed genomic regions. This approach has been widely adopted for genome-scale bisulfite…
View article: Reduced representation optical methylation mapping (R<sup>2</sup>OM<sup>2</sup>)
Reduced representation optical methylation mapping (R<sup>2</sup>OM<sup>2</sup>) Open
Reduced representation methylation analysis utilizes a subset of CpGs in order to report the overall methylation status of the probed genomic regions. Here, we use this concept in order to create fluorescent optical methylation profiles al…