Domenico Galante
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View article: Defining epidemiological cutoff values for Brucella melitensis: an European multicentre study
Defining epidemiological cutoff values for Brucella melitensis: an European multicentre study Open
View article: Observed Prevalence and Characterization of Fluoroquinolone-Resistant and Multidrug-Resistant Bacteria in Loggerhead Sea Turtles (Caretta caretta) from the Adriatic Sea
Observed Prevalence and Characterization of Fluoroquinolone-Resistant and Multidrug-Resistant Bacteria in Loggerhead Sea Turtles (Caretta caretta) from the Adriatic Sea Open
Background/Objectives: Antimicrobial resistance (AMR) is a major global health concern with profound implications for human, animal, and environmental health. Marine ecosystems are emerging as reservoirs of resistant bacteria due to contam…
View article: Identification of <i>Actinobacillus seminis</i> as the cause of abortion in sheep by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and whole genome sequencing
Identification of <i>Actinobacillus seminis</i> as the cause of abortion in sheep by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and whole genome sequencing Open
Actinobacillus seminis is a gram-negative bacterium that affects reproductive organs, causing epididymitis, low fertility, and occasional abortions in sheep and goats. The virulence factors and the pathogenicity mechanisms of A. seminis ha…
View article: Prevalence and Antimicrobial Resistance of Salmonella Strains Isolated from Chicken Samples in Southern Italy
Prevalence and Antimicrobial Resistance of Salmonella Strains Isolated from Chicken Samples in Southern Italy Open
Salmonellosis is one of the most frequent foodborne zoonoses, and the consumption of contaminated poultry meat is considered the main source of Salmonella infections in humans. From 2021 to 2023, 384 chicken samples were collected from ret…
View article: Isolation and Characterization of Colistin-Resistant Enterobacteriaceae from Foods in Two Italian Regions in the South of Italy
Isolation and Characterization of Colistin-Resistant Enterobacteriaceae from Foods in Two Italian Regions in the South of Italy Open
The emergence of colistin-resistant Enterobacteriaceae in food products is a growing concern due to the potential transfer of resistance to human pathogens. This study aimed to assess the prevalence of colistin-resistant Enterobacteriaceae…
View article: Identification of Bacillus anthracis Strains from Animal Cases in Ethiopia and Genetic Characterization by Whole-Genome Sequencing
Identification of Bacillus anthracis Strains from Animal Cases in Ethiopia and Genetic Characterization by Whole-Genome Sequencing Open
Anthrax is a zoonotic disease characterized by rapid onset with usual fatal outcomes in livestock and wildlife. In Ethiopia, anthrax is a persistent disease; however, there are limited data on the isolation and molecular characterization o…
View article: Antimicrobial and Phylogenomic Characterization of Bacillus cereus Group Strains Isolated from Different Food Sources in Italy
Antimicrobial and Phylogenomic Characterization of Bacillus cereus Group Strains Isolated from Different Food Sources in Italy Open
Background: Bacillus cereus is a widespread environmental Gram-positive bacterium which is especially common in soil and dust. It produces two types of toxins that cause vomiting and diarrhea. At present, foodborne outbreaks due to Bacillu…
View article: Antimicrobial Characterization of<em> Bacillus cereus </em>Group Strains Isolated from Different Food Sources in Apulia and Basilicata Regions
Antimicrobial Characterization of<em> Bacillus cereus </em>Group Strains Isolated from Different Food Sources in Apulia and Basilicata Regions Open
Bacillus cereus is a gram-positive bacterium widespread in the environment, especially in soil and dust. It produces two types of toxins causing vomiting and diarrhea. Nowadays, food borne outbreaks due to Bacillus cereus group bacteria (e…
View article: In field evaluation of impact on clinical signs of an inactivated autogenous vaccine against <i>Pasteurella multocida</i> in rabbits
In field evaluation of impact on clinical signs of an inactivated autogenous vaccine against <i>Pasteurella multocida</i> in rabbits Open
In Italy, the use of autogenous inactivated vaccines prepared with the bacterial strains isolated from affected animals is authorized by the Ministry of Health in farms where bacterial diseases occur frequently. The autogenous vaccine perf…
View article: Environmental relationships and anthrax epidemiology: field experiences of host resistance as opposed to dose-dependent experiments
Environmental relationships and anthrax epidemiology: field experiences of host resistance as opposed to dose-dependent experiments Open
Even though anthrax is a disease of antiquity that has been studied for centuries, serious concerns have been raised about our understanding of its epidemiology. Since the 1960s, we have based the epidemiology of anthrax on the results of …
View article: Application of Fourier Transform Infrared Spectroscopy to Discriminate Two Closely Related Bacterial Species: Bacillus anthracis and Bacillus cereus Sensu Stricto
Application of Fourier Transform Infrared Spectroscopy to Discriminate Two Closely Related Bacterial Species: Bacillus anthracis and Bacillus cereus Sensu Stricto Open
Fourier transform infrared spectroscopy (FTIRS) is a diagnostic technique historically used in the microbiological field for the characterization of bacterial strains in relation to the specific composition of their lipid, protein, and pol…
View article: Retrospective Analysis of Official Data on Anthrax in Europe with a Special Reference to Ukraine
Retrospective Analysis of Official Data on Anthrax in Europe with a Special Reference to Ukraine Open
Anthrax is an acute infectious zoonotic disease caused by Bacillus anthracis that mostly affects grazing livestock and wildlife. Furthermore, B. anthracis is considered one of the most important biological agents of bioterrorism that could…
View article: Environmental Relationships and Anthrax Epidemiology: Field Experiences of Host Resistance as Opposed to Dose-Dependent Experiments
Environmental Relationships and Anthrax Epidemiology: Field Experiences of Host Resistance as Opposed to Dose-Dependent Experiments Open
View article: Evaluation of a real-time PCR in combination with a cultivation method for the detection of <i>Brucella abortus</i> in organs of infected cattle in southern Italy
Evaluation of a real-time PCR in combination with a cultivation method for the detection of <i>Brucella abortus</i> in organs of infected cattle in southern Italy Open
Introduction Brucellosis is a widespread zoonosis of great economic importance for livestock farming in many areas of the world. It is a highly infectious disease which is diagnosed using conventional serological and microbiological method…
View article: SARS-CoV-2 RNA viability on high-touch surfaces and evaluation of a continuous-flow ozonation treatment.
SARS-CoV-2 RNA viability on high-touch surfaces and evaluation of a continuous-flow ozonation treatment. Open
Although no important changes were observed by treating the environments with ozonisation systems, further studies are needed to validate the effectiveness of environmental treatments with airborne disinfectants.
View article: Identification of Universally Applicable and Species-Specific Marker Peptides for Bacillus anthracis
Identification of Universally Applicable and Species-Specific Marker Peptides for Bacillus anthracis Open
Anthrax is a zoonotic infection caused by the bacterium Bacillus anthracis (BA). Specific identification of this pathogen often relies on targeting genes located on two extrachromosomal plasmids, which represent the major pathogenicity fac…
View article: Toxigenic Genes, Pathogenic Potential and Antimicrobial Resistance of Bacillus cereus Group Isolated from Ice Cream and Characterized by Whole Genome Sequencing
Toxigenic Genes, Pathogenic Potential and Antimicrobial Resistance of Bacillus cereus Group Isolated from Ice Cream and Characterized by Whole Genome Sequencing Open
Bacillus cereus is isolated from a variety of foods where it may cause food spoilage and/or food poisoning due to its toxigenic and pathogenic nature. In this study, we identified members of B. cereus groups in 65% of the ice cream samples…
View article: Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method
Adaptation of Brucella melitensis Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method Open
Brucellosis, mainly caused by Brucella (B.) melitensis, is associated with a risk of chronification and relapses. Antimicrobial susceptibility testing (AST) standards for B. melitensis are not available, and the agent is not yet listed in …
View article: Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods
Isolation and characterization of Yersinia enterocolitica from foods in Apulia and Basilicata regions (Italy) by conventional and modern methods Open
Yersiniosis is the third most reported food-borne zoonosis in Europe. The aim of the present study was to perform the search for Yersinia enterocolitica in food samples collected from Apulia and Basilicata regions (Southern Italy) and to c…
View article: An Outbreak of Human Systemic Anthrax, including One Case of Anthrax Meningitis, Occurred in Calabria Region (Italy): A Description of a Successful One Health Approach
An Outbreak of Human Systemic Anthrax, including One Case of Anthrax Meningitis, Occurred in Calabria Region (Italy): A Description of a Successful One Health Approach Open
In this report, three cases of human cutaneous anthrax are described, one complicated by meningitis, and all were linked to a single infected bullock. A 41-year-old male truck driver, along with two male slaughterhouse workers, 45 and 42, …
View article: A challenging case of Francisella Tularensis: teenager’s massive lymphoadenopathy caused by an uncommon infectious disease.
A challenging case of Francisella Tularensis: teenager’s massive lymphoadenopathy caused by an uncommon infectious disease. Open
Tularemia is a potentially fatal, multisystemic infectious disease of humans and some animals caused by Fran- cisella Tularensis bacteria. Up to six forms of clinical manifestations of tularemia have been identified, ulcer- oglandular tula…
View article: SARS-CoV-2 Gamma and Delta Variants of Concern Might Undermine Neutralizing Activity Generated in Response to BNT162b2 mRNA Vaccination
SARS-CoV-2 Gamma and Delta Variants of Concern Might Undermine Neutralizing Activity Generated in Response to BNT162b2 mRNA Vaccination Open
The Delta variant raised concern regarding its ability to evade SARS-CoV-2 vaccines. We evaluated a serum neutralizing response of 172 Italian healthcare workers, three months after complete Comirnaty (BNT162b2 mRNA, BioNTech-Pfizer) vacci…
View article: Production of a Bacillus anthracis Secretome with Suitable Characteristics as Antigen in a Complement Fixation Test
Production of a Bacillus anthracis Secretome with Suitable Characteristics as Antigen in a Complement Fixation Test Open
In this study, we cultured the Bacillus anthracis vaccine strain Sterne 34F2 in a medium containing EDTA, and we assessed the best conditions to inhibit the activity of zinc-dependent metalloproteases to obtain a secretome containing a hig…
View article: Do We Really Need Hazard Prevention at the Expense of Safeguarding Death Dignity in COVID-19?
Do We Really Need Hazard Prevention at the Expense of Safeguarding Death Dignity in COVID-19? Open
To date, little is known regarding the transmission risks of SARS-CoV-2 infection for subjects involved in handling, transporting, and examining deceased persons with known or suspected COVID-19 positivity at the time of death. This experi…
View article: Klebsiella pneumoniae in vegetable products: antimicrobial resistance and consumer health risk
Klebsiella pneumoniae in vegetable products: antimicrobial resistance and consumer health risk Open
Background Klebsiella pneumoniae (Kp) is considered one of the major causes of human hospital infections. Moreover, Kp is often present in several food products and it could be a potential health hazard for the consumers through the increa…
View article: Virulence and antibiotic resistance of Klebsiella pneumoniae strains isolated from wastewater
Virulence and antibiotic resistance of Klebsiella pneumoniae strains isolated from wastewater Open
Background Multidrug-resistant Klebsiella pneumoniae (Kp) strains are considered a global public health threat. The aim of the present study was to assess the occurrence of resistant and virulent Kp in Italian environmental wastewater, in …
View article: Genome Sequence Analysis of Clostridium chauvoei Strains of European Origin and Evaluation of Typing Options for Outbreak Investigations
Genome Sequence Analysis of Clostridium chauvoei Strains of European Origin and Evaluation of Typing Options for Outbreak Investigations Open
Black quarter caused by Clostridium (C.) chauvoei is an important bacterial disease that affects cattle and sheep with high mortality. A comparative genomics analysis of 64 C. chauvoei strains, most of European origin and a few of non-Euro…
View article: Discrimination of Bacillus cereus Group Members by MALDI-TOF Mass Spectrometry
Discrimination of Bacillus cereus Group Members by MALDI-TOF Mass Spectrometry Open
Matrix-Assisted Laser Desorption/Ionization Time Of Flight Mass Spectrometry (MALDI-TOF MS) technology is currently increasingly used in diagnostic laboratories as a cost effective, rapid and reliable routine technique for the identificati…
View article: Genomic Surveillance of Circulating SARS-CoV-2 in South East Italy: A One-Year Retrospective Genetic Study
Genomic Surveillance of Circulating SARS-CoV-2 in South East Italy: A One-Year Retrospective Genetic Study Open
In order to provide insights into the evolutionary and epidemiological viral dynamics during the current COVID-19 pandemic in South Eastern Italy, a total of 298 genomes of SARS-CoV-2 strains collected in the Apulia and Basilicata regions,…
View article: Investigation on Anthrax in Bangladesh during the Outbreaks of 2011 and Definition of the Epidemiological Correlations
Investigation on Anthrax in Bangladesh during the Outbreaks of 2011 and Definition of the Epidemiological Correlations Open
In 2011, in Bangladesh, 11 anthrax outbreaks occurred in six districts of the country. Different types of samples were collected from May to September in the six districts where anthrax had occurred in order to detect and type Bacillus ant…