Dominic J. Wells
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View article: Spatiotemporal analysis of dystrophin expression during muscle repair
Spatiotemporal analysis of dystrophin expression during muscle repair Open
View article: Long-term, age-associated activity quantification in the DE50-MD dog model of Duchenne muscular dystrophy
Long-term, age-associated activity quantification in the DE50-MD dog model of Duchenne muscular dystrophy Open
Animal models with a clinically relevant phenotype remain important for robust evaluation of novel therapeutics for the fatal X-linked genetic disorder Duchenne muscular dystrophy (DMD). Demonstration of functional improvement is crucial f…
View article: Long-term, age-associated activity quantification in the DE50-MD dog model of Duchenne muscular dystrophy (DMD)
Long-term, age-associated activity quantification in the DE50-MD dog model of Duchenne muscular dystrophy (DMD) Open
Animal models with a clinically relevant phenotype remain important for robust evaluation of novel therapeutics for the fatal, X-linked genetic disorder, Duchenne Muscular Dystrophy (DMD). Demonstration of functional improvement is crucial…
View article: Evaluation of a six-minute walk test in the DE50-MD canine model of Duchenne muscular dystrophy and its effect on blood-borne biomarkers
Evaluation of a six-minute walk test in the DE50-MD canine model of Duchenne muscular dystrophy and its effect on blood-borne biomarkers Open
Background Duchenne muscular dystrophy (DMD) is a fatal muscle wasting disease caused by mutations in the dystrophin gene resulting in cycles of muscle degeneration, inflammation and regeneration. The 6-minute walk test (6MWT) is a key fun…
View article: Progression of the cardiac phenotype of the DE50-MD dog model of Duchenne Muscular Dystrophy, corroborating results of cardiac magnetic resonance imaging with pathology up to 36 months of age
Progression of the cardiac phenotype of the DE50-MD dog model of Duchenne Muscular Dystrophy, corroborating results of cardiac magnetic resonance imaging with pathology up to 36 months of age Open
Cardiomyopathy is an expected consequence of the invariably fatal, X-linked muscle-wasting disease, Duchenne Muscular Dystrophy, (DMD) but onset and progression vary between individuals. Cardiac magnetic resonance imaging (CMR) is invaluab…
View article: The preclinical cardiac phenotype of the DE50-MD dog model of Duchenne muscular dystrophy
The preclinical cardiac phenotype of the DE50-MD dog model of Duchenne muscular dystrophy Open
Cardiomyopathy is the leading cause of death in the X-linked disorder, Duchenne Muscular Dystrophy (DMD) yet optimal management strategies remain undetermined. Advances in the search for novel DMD treatments, particularly at cell and molec…
View article: Spatiotemporal analysis of dystrophin expression during muscle repair
Spatiotemporal analysis of dystrophin expression during muscle repair Open
Dystrophin mRNA is produced from a very large genetic locus and transcription of a single mRNA requires approximately 16 hours. This prolonged interval between transcriptional initiation and completion results in unusual transcriptional be…
View article: Identification of reference microRNAs in skeletal muscle of a canine model of Duchenne muscular dystrophy
Identification of reference microRNAs in skeletal muscle of a canine model of Duchenne muscular dystrophy Open
Background Duchenne muscular dystrophy (DMD) is a fatal muscle wasting disease caused by mutations in the dystrophin gene. DE50-MD dogs are an animal model of DMD used as a final translational model for evaluation of promising treatments. …
View article: Evaluation of a six-minute walk test in the DE50-MD canine model of Duchenne muscular dystrophy and its effect on blood-borne biomarkers
Evaluation of a six-minute walk test in the DE50-MD canine model of Duchenne muscular dystrophy and its effect on blood-borne biomarkers Open
Background Duchenne muscular dystrophy (DMD) is a fatal muscle wasting disease caused by mutations in the dystrophin gene resulting in cycles of muscle degeneration, inflammation and regeneration. The 6-minute walk test (6MWT) is a key fun…
View article: Identification of reference microRNAs in skeletal muscle of a canine model of Duchenne muscular dystrophy
Identification of reference microRNAs in skeletal muscle of a canine model of Duchenne muscular dystrophy Open
Background Duchenne muscular dystrophy (DMD) is a fatal muscle wasting disease caused by mutations in the dystrophin gene. DE50-MD dogs are a canine model of DMD used as final translational models for evaluation of promising treatments. Mi…
View article: Ion channels as biomarkers of altered myogenesis in myofiber precursors of Duchenne muscular dystrophy
Ion channels as biomarkers of altered myogenesis in myofiber precursors of Duchenne muscular dystrophy Open
Myogenesis is essential for skeletal muscle formation, growth, and regeneration and can be altered in Duchenne muscular dystrophy (DMD), an X‐linked disorder due to the absence of the cytoskeletal protein dystrophin. Ion channels play a pi…
View article: Longitudinal assessment of skeletal muscle functional mechanics in the DE50-MD dog model of Duchenne muscular dystrophy
Longitudinal assessment of skeletal muscle functional mechanics in the DE50-MD dog model of Duchenne muscular dystrophy Open
Duchenne muscular dystrophy (DMD), caused by mutations in the dystrophin (DMD) gene, is associated with fatal muscle degeneration and atrophy. Patients with DMD have progressive reductions in skeletal muscle strength and resistance to ecce…
View article: Mouse Brain qPCR data
Mouse Brain qPCR data Open
Cq and RQ data for qPCR analysis of wildtype and mdx mouse brains: assessment of a panel of potential reference genes.Data for manuscript "Identification of quantitative polymerase chain reaction reference genes suitable for normalizing ge…
View article: Serum inflammatory cytokines as disease biomarkers in the DE50-MD dog model of Duchenne muscular dystrophy
Serum inflammatory cytokines as disease biomarkers in the DE50-MD dog model of Duchenne muscular dystrophy Open
Duchenne muscular dystrophy (DMD) is a fatal muscle-wasting disease, caused by mutations in the dystrophin gene, characterised by cycles of muscle degeneration, inflammation and regeneration. Recently, there has been renewed interest speci…
View article: The skeletal muscle phenotype of the DE50-MD dog model of Duchenne muscular dystrophy
The skeletal muscle phenotype of the DE50-MD dog model of Duchenne muscular dystrophy Open
Background: Animal models of Duchenne muscular dystrophy (DMD) are essential to study disease progression and assess efficacy of therapeutic intervention, however dystrophic mice fail to display a clinically relevant phenotype, limiting tr…
View article: Identification of qPCR reference genes suitable for normalising gene expression in the developing mouse embryo
Identification of qPCR reference genes suitable for normalising gene expression in the developing mouse embryo Open
Background: Progression through mammalian embryogenesis involves many interacting cell types and multiple differentiating cell lineages. Quantitative polymerase chain reaction (qPCR) analysis of gene expression in the developing embryo is …
View article: Longitudinal assessment of blood-borne musculoskeletal disease biomarkers in the DE50-MD dog model of Duchenne muscular dystrophy
Longitudinal assessment of blood-borne musculoskeletal disease biomarkers in the DE50-MD dog model of Duchenne muscular dystrophy Open
Background: Duchenne muscular dystrophy (DMD) is a fatal muscle wasting disease caused by mutations in the dystrophin gene. Due to their phenotypic similarity to human patients, large animal models are invaluable tools for pre-clinical tri…
View article: Rapid histological quantification of muscle fibrosis and lysosomal activity using the HSB colour space
Rapid histological quantification of muscle fibrosis and lysosomal activity using the HSB colour space Open
Introduction Fibrosis is a key feature of many chronic myopathic disorders, such as in the muscle-wasting condition, Duchenne muscular dystrophy. Fibrosis disrupts skeletal muscle architecture, limits muscle function, impairs regeneration …
View article: Validation of DE50-MD dogs as a model for the brain phenotype of Duchenne muscular dystrophy
Validation of DE50-MD dogs as a model for the brain phenotype of Duchenne muscular dystrophy Open
Duchenne muscular dystrophy (DMD), a fatal musculoskeletal disease, is associated with neurodevelopmental disorders and cognitive impairment caused by brain dystrophin deficiency. Dog models of DMD represent key translational tools to stud…
View article: Raw data: DE50-MD Serum inflammatory cytokines - Riddell Hildyard Harron Hornby Wells Piercy
Raw data: DE50-MD Serum inflammatory cytokines - Riddell Hildyard Harron Hornby Wells Piercy Open
Folder contains raw data for Serum inflammatory cytokines as disease biomarkers in the DE50-MD dog model of Duchenne muscular dystrophy• Luminex raw data• Serum CCL2 vs CK activity raw data• CCL2 CCR2 mRNA raw data - Legend: orange cells r…
View article: Raw data: DE50-MD Serum inflammatory cytokines - Riddell Hildyard Harron Hornby Wells Piercy
Raw data: DE50-MD Serum inflammatory cytokines - Riddell Hildyard Harron Hornby Wells Piercy Open
Folder contains raw data for Serum inflammatory cytokines as disease biomarkers in the DE50-MD dog model of Duchenne muscular dystrophy • Luminex raw data • Correlation between serum CCL2 and other biomarkers of DE50-MD Serum CCL2 vs pla…
View article: Longitudinal assessment of blood-borne musculoskeletal disease biomarkers in the DE50-MD dog model of Duchenne muscular dystrophy
Longitudinal assessment of blood-borne musculoskeletal disease biomarkers in the DE50-MD dog model of Duchenne muscular dystrophy Open
Background: Duchenne muscular dystrophy (DMD) is a fatal muscle wasting disease caused by mutations in the dystrophin gene. Due to their phenotypic similarity to human patients, large animal models are invaluable tools for pre-clinical tr…
View article: Identification of qPCR reference genes suitable for normalising gene expression in the developing mouse embryo
Identification of qPCR reference genes suitable for normalising gene expression in the developing mouse embryo Open
Background: Progression through mammalian embryogenesis involves many interacting cell types and multiple differentiating cell lineages. Quantitative polymerase chain reaction (qPCR) analysis of gene expression in the developing embryo is …
View article: The Pen Is Milder Than the Blade: Identification Marking Mice Using Ink on the Tail Appears More Humane Than Ear-Punching Even with Local Anaesthetic
The Pen Is Milder Than the Blade: Identification Marking Mice Using Ink on the Tail Appears More Humane Than Ear-Punching Even with Local Anaesthetic Open
Identification marking mice commonly involves ear-punching with or without anaesthetic, or tail-marking with ink. To identify which is most humane, we marked weanling male BALB/c mice using ear-punching (EP), ear-punching with anaesthetic …
View article: Musculoskeletal magnetic resonance imaging in the DE50-MD dog model of Duchenne muscular dystrophy
Musculoskeletal magnetic resonance imaging in the DE50-MD dog model of Duchenne muscular dystrophy Open
The DE50-MD canine model of Duchenne muscular dystrophy (DMD) has a dystrophin gene splice site mutation causing deletion of exon 50, an out-of-frame transcript and absence of dystrophin expression in striated muscles. We hypothesized that…
View article: Author’s Response to: Rebuttal to: Simvastatin Treatment Does Not Ameliorate Muscle Pathophysiology in a Mouse Model for Duchenne Muscular Dystrophy, Verhaart et al. 2020
Author’s Response to: Rebuttal to: Simvastatin Treatment Does Not Ameliorate Muscle Pathophysiology in a Mouse Model for Duchenne Muscular Dystrophy, Verhaart et al. 2020 Open
View article: Identification of quantitative polymerase chain reaction reference genes suitable for normalising gene expression in the brain of normal and dystrophic mice and dogs
Identification of quantitative polymerase chain reaction reference genes suitable for normalising gene expression in the brain of normal and dystrophic mice and dogs Open
Background: In addition to progressive, debilitating muscle degeneration, ~50% of patients with Duchenne muscular dystrophy (DMD) have associated cognitive and behavioural disorders secondary to deficiency of dystrophin protein in the brai…
View article: Mouse Brain qPCR data
Mouse Brain qPCR data Open
Cq and RQ data for qPCR analysis of wildtype and mdx mouse brains: assessment of a panel of potential reference genes.Data for manuscript "Identification of quantitative polymerase chain reaction reference genes suitable for normalizing ge…
View article: Embryo qPCR reference genes: animal numbers and sample sizes
Embryo qPCR reference genes: animal numbers and sample sizes Open
This file details the precise animal numbers and sample allocations used for the manuscriptIdentification of qPCR reference genes suitable for normalising gene expression in the developing mouse embryoJohn C.W. Hildyard, Dominic J. …
View article: Dog Brain qPCR data
Dog Brain qPCR data Open
Rq and CQ qPCR data for wildtype and DE50-MD (Muscular dystrophy model) dog brain: assessment of panel of potential reference genes for normalisationData for manuscript entitled "Identification of quantitative polymerase chain reacti…