Don C. Mackel
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View article: Factors associated with Pseudomonas pickettii intrinsic contamination of commercial respiratory therapy solutions marketed as sterile
Factors associated with Pseudomonas pickettii intrinsic contamination of commercial respiratory therapy solutions marketed as sterile Open
Laboratory investigations were conducted to study the growth dynamics of Pseudomonas pickettii in commercial 0.9% sodium chloride solution under various environmental conditions and to determine the retention of these organisms after chall…
View article: Investigations into the survival of Pseudomonas aeruginosa in poloxamer-iodine
Investigations into the survival of Pseudomonas aeruginosa in poloxamer-iodine Open
Laboratory investigations were conducted to study potential mechanisms for prolonged survival of Pseudomonas aeruginosa in poloxamer-iodine (PxI). P. aeruginosa organisms isolated from PxI and adapted for growth in distilled water or found…
View article: Carpeting in hospitals: an epidemiological evaluation
Carpeting in hospitals: an epidemiological evaluation Open
Epidemiological and microbiological studies were conducted in a hospital room with carpet (CR) and in one with carpet (NCR). Microbiological profiles were determined with specimens obtained from patients admitted to these rooms. Patient re…
View article: Failure of Legionella pneumophila sensitivities to predict culture results from disinfectant-treated air-conditioning cooling towers
Failure of Legionella pneumophila sensitivities to predict culture results from disinfectant-treated air-conditioning cooling towers Open
The disinfection of cooling towers based on manufacturers' treatment protocols, as employed in units installed at various public gathering places in Dallas, Tex. (hotels, municipal auditorium), and at the city health department, was evalua…
View article: Laboratory studies of disinfectants against Legionella pneumophila
Laboratory studies of disinfectants against Legionella pneumophila Open
Legionella pneumophila suspended in tap water was exposed to biocides recommended for inhibiting biological growth in cooling towers and evaporative condensers of air-conditioning systems. Chlorine, 2,2-dibromo-3-nitrilopropionamide, and a…
View article: Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila
Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila Open
Charcoal-yeast extract agar is a new bacteriological medium that supports excellent growth of the Legionella pneumophila. It results from modifications made in an existing L. pneumophila medium, F-G agar. Yeast extract, instead of an acid …
View article: Exotoxin activity associated with the Legionnaires disease bacterium
Exotoxin activity associated with the Legionnaires disease bacterium Open
Hemolysis occurred around growth of the Legionnaires disease bacterium on supplemented Mueller-Hinton agar containing sterile defibrinated blood from each of five mammalian species. Hemolysis was most pronounced with guinea pig or rabbit b…
View article: Primary isolation media for Legionnaires disease bacterium
Primary isolation media for Legionnaires disease bacterium Open
Yolk sac suspensions infected with the Legionnaires disease bacterium (LDB) were plated onto 17 different bacteriological agar media. The LDB grew only on Mueller-Hinton agar supplemented with 1% Iso Vitale X and 1% hemoglobin (MH-IH). Thi…
View article: Nationwide epidemic of septicemia caused by contaminated intravenous products: mechanisms of intrinsic contamination
Nationwide epidemic of septicemia caused by contaminated intravenous products: mechanisms of intrinsic contamination Open
Between 1 July 1970 and April 1971, in many hospitals in this country, there were outbreaks of nosocomial septicemia caused by Enterobacter cloacae of E. agglomerans (formerly Erwinia, herbicola-lathyri). All of these hospitals used infusi…
View article: Microbial Penetration Through Three Types of Double Wrappers for Sterile Packs
Microbial Penetration Through Three Types of Double Wrappers for Sterile Packs Open
Microbial penetration of sterile packs was studied by using double-wrap (two layers each) muslin, single-wrap (two layers) muslin inner covering with single-wrap (one layer) two-way crepe paper outer covering, and single-wrap (two layers) …
View article: Microbial Penetration Through Three Types of Double Wrappers for Sterile Packs
Microbial Penetration Through Three Types of Double Wrappers for Sterile Packs Open
Microbial penetration of sterile packs was studied by using double-wrap (two layers each) muslin, single-wrap (two layers) muslin inner covering with single-wrap (one layer) two-way crepe paper outer covering, and single-wrap (two layers) …
View article: Microbial Penetration of Muslin- and Paper-Wrapped Sterile Packs Stored on Open Shelves and in Closed Cabinets
Microbial Penetration of Muslin- and Paper-Wrapped Sterile Packs Stored on Open Shelves and in Closed Cabinets Open
Microbial penetration of sterile packs was studied using single-wrap (two layers) muslin, double-wrap (four layers) muslin, and two-way crepe paper (single layer) to wrap 20 gauze sponges (2 by 2 inch). These packs were stored in the centr…