Dongbang Yao
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View article: Development and construction of a novel Bacillus subtilis autoinducible extracellular expression system based on a LuxI/R device
Development and construction of a novel Bacillus subtilis autoinducible extracellular expression system based on a LuxI/R device Open
To our knowledge, this is the first study to report enhanced autoinducible expression of the lux system in B. subtilis by engineering the sensing module promoter SPluxI sequence and the lux box copy number of the response module promoter R…
View article: Enhanced extracellular production of Coprinopsis cinerea laccase Lcc9 in Aspergillus niger by gene expression cassette and bioprocess optimization
Enhanced extracellular production of Coprinopsis cinerea laccase Lcc9 in Aspergillus niger by gene expression cassette and bioprocess optimization Open
To our knowledge, this study is the first to report the recombinant extracellular production of the C. cinerea laccase Lcc9 in A. niger and to use SPCAT in the A. niger expression system. The results of this study will help accelerate the …
View article: Enhanced extracellular production of raw starch-degrading α-amylase in Bacillus subtilis through expression regulatory element modification and fermentation optimization
Enhanced extracellular production of raw starch-degrading α-amylase in Bacillus subtilis through expression regulatory element modification and fermentation optimization Open
Background Raw starch-degrading α-amylase (RSDA) can hydrolyze raw starch at moderate temperatures, thus contributing to savings in starch processing costs. However, the low production level of RSDA limits its industrial application. There…
View article: Additional file 2 of Enhanced extracellular production of raw starch-degrading α-amylase in Bacillus subtilis through expression regulatory element modification and fermentation optimization
Additional file 2 of Enhanced extracellular production of raw starch-degrading α-amylase in Bacillus subtilis through expression regulatory element modification and fermentation optimization Open
Additional file 2: Table S1 Sequence characteristics of signal peptides.
View article: Enhanced extracellular raw starch-degrading α-amylase production in Bacillus subtilis through signal peptide and translation efficiency optimization
Enhanced extracellular raw starch-degrading α-amylase production in Bacillus subtilis through signal peptide and translation efficiency optimization Open
A strategy that combines signal peptide (SP) optimization and translation efficiency improvement was used to enhance the extracellular production of raw starch-degrading α-amylase AmyZ1 from Pontibacillus sp. ZY in Bacillus subtilis. The o…
View article: Enhanced extracellular α-amylase production in <i>Brevibacillus choshinensis</i> by optimizing extracellular degradation and folding environment
Enhanced extracellular α-amylase production in <i>Brevibacillus choshinensis</i> by optimizing extracellular degradation and folding environment Open
A strategy for optimizing the extracellular degradation and folding environment of Brevibacillus choshinensis has been used to enhance the extracellular production of recombinant α-amylase. First, a gene (bcp) encoding an extracellular pro…
View article: Enhanced Production of Soluble <i>Pyrococcus furiosus</i> α-Amylase in <i>Bacillus subtilis</i> through Chaperone Co-Expression, Heat Treatment and Fermentation Optimization
Enhanced Production of Soluble <i>Pyrococcus furiosus</i> α-Amylase in <i>Bacillus subtilis</i> through Chaperone Co-Expression, Heat Treatment and Fermentation Optimization Open
Pyrococcus furiosus α-amylase can hydrolyze α-1,4 linkages in starch and related carbohydrates under hyperthermophilic condition (~ 100°C), showing great potential in a wide range of industrial applications, while its relatively low produc…