Diana G. Eng
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View article: Isolation of Podocyte Cell Fractions From Mouse Kidney Using Magnetic Activated Cell Sorting (MACS)
Isolation of Podocyte Cell Fractions From Mouse Kidney Using Magnetic Activated Cell Sorting (MACS) Open
Glomerular diseases characterized by injury to post-mitotic epithelial cells called podocytes are a leading cause of chronic kidney disease. Yet, isolating podocytes from the kidney for transcriptomic, proteomic, and metabolomic studies ha…
View article: Nanoscale Optical Imaging, Reconstruction, and Spatial Analysis of Whole Mouse Glomeruli
Nanoscale Optical Imaging, Reconstruction, and Spatial Analysis of Whole Mouse Glomeruli Open
Renal glomeruli have traditionally been studied by micrometer-scale optical microscopy to interrogate overall physiology or molecular distributions and by nanoscale electron microscopy to interrogate the ultrastructure of thin sections. Wh…
View article: Podocytes from hypertensive and obese mice acquire an inflammatory, senescent, and aged phenotype
Podocytes from hypertensive and obese mice acquire an inflammatory, senescent, and aged phenotype Open
Hypertension and obesity can lead to glomerular dysfunction in patients, causing podocyte injury and depletion. Here, young mice given deoxycorticosterone acetate or a high-fat diet to induce hypertension or obesity, respectively. mRNA seq…
View article: Podocyte injury at young age causes premature senescence and worsens glomerular aging
Podocyte injury at young age causes premature senescence and worsens glomerular aging Open
Glomerular function is decreased by aging. However, little is known about the molecular mechanisms involved in age-related glomerular changes and which factors could contribute to a worse glomerular aging process. Here, we reported that po…
View article: Inhibiting NLRP3 signaling in aging podocytes improves their life- and health-span
Inhibiting NLRP3 signaling in aging podocytes improves their life- and health-span Open
The decrease in the podocyte's lifespan and health-span that typify healthy kidney aging cause a decrease in their normal structure, physiology and function. The ability to halt and even reverse these changes becomes clinically relevant wh…
View article: Upregulated PD-1 signaling antagonizes glomerular health in aged kidneys and disease
Upregulated PD-1 signaling antagonizes glomerular health in aged kidneys and disease Open
With an aging population, kidney health becomes an important medical and socioeconomic factor. Kidney aging mechanisms are not well understood. We previously showed that podocytes isolated from aged mice exhibit increased expression of pro…
View article: Upregulated PD-1 Signaling is an Important Antagonist to Glomerular Health in Aged Kidneys
Upregulated PD-1 Signaling is an Important Antagonist to Glomerular Health in Aged Kidneys Open
Kidney aging and its contribution to disease and its underlying mechanisms are not well understood. With an aging population, kidney health becomes an important medical and socioeconomic factor. We previously showed that podocytes isolated…
View article: Parietal epithelial cell differentiation to a podocyte fate in the aged mouse kidney
Parietal epithelial cell differentiation to a podocyte fate in the aged mouse kidney Open
Healthy aging is typified by a progressive and absolute loss of podocytes over the lifespan of animals and humans. To test the hypothesis that a subset of glomerular parietal epithelial cell (PEC) progenitors transition to a podocyte fate …
View article: Nanoparticles exhibit greater accumulation in kidney glomeruli during experimental glomerular kidney disease
Nanoparticles exhibit greater accumulation in kidney glomeruli during experimental glomerular kidney disease Open
Loss and dysfunction of glomerular podocytes result in increased macromolecule permeability through the glomerular filtration barrier and nephrotic syndrome. Current therapies can induce and maintain disease remission, but cause serious an…
View article: CD44 impacts glomerular parietal epithelial cell changes in the aged mouse kidney
CD44 impacts glomerular parietal epithelial cell changes in the aged mouse kidney Open
CD44 contributes to the activation of glomerular parietal epithelial cells (PECs). Although CD44 expression is higher in PECs of healthy aged mice, the biological role of CD44 in PECs in this context remains unclear. Accordingly, young (4 …
View article: Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy
Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy Open
In healthy glomeruli, parietal epithelial cell (PEC)-derived extracellular matrix (ECM) proteins include laminin-β 1 , perlecan, and collagen type IV-α 2 and podocyte-specific ECM proteins include laminin-β 2 , agrin, and collagen type IV-…
View article: Cells of NG2 lineage increase in glomeruli of mice following podocyte depletion
Cells of NG2 lineage increase in glomeruli of mice following podocyte depletion Open
Under certain circumstances, podocytes can be partially replaced following their loss in disease. The inability of podocytes to proliferate suggests that replacement derives from other cell types. Because neural/glial antigen 2 (NG2)-expre…
View article: Sex differences in transcriptomic profiles in aged kidney cells of renin lineage
Sex differences in transcriptomic profiles in aged kidney cells of renin lineage Open
Renin expressing cells in the kidney's juxta-glomeruluar compartment likely also serve as progenitors for adult glomerular cells in disease. Although these cells of renin lineage (CoRL) decrease in number with advancing kidney age, accompa…
View article: Lineage tracing aged mouse kidneys shows lower number of cells of renin lineage and reduced responsiveness to RAAS inhibition
Lineage tracing aged mouse kidneys shows lower number of cells of renin lineage and reduced responsiveness to RAAS inhibition Open
Blocking the renin-angiotensin-aldosterone system (RAAS) remains a mainstay of therapy in hypertension and glomerular diseases. With the population aging, our understanding of renin-producing cells in kidneys with advanced age is more crit…
View article: Charting the transcriptional landscape of cells of renin lineage following podocyte depletion
Charting the transcriptional landscape of cells of renin lineage following podocyte depletion Open
Renin producing cells of the juxtaglomerulus, herein called cells of renin lineage (CoRL), have garnered recent interest for their propensity to act as a progenitor source for various kidney cell types including podocytes. Despite recent a…
View article: Tracking the stochastic fate of cells of the renin lineage after podocyte depletion using multicolor reporters and intravital imaging
Tracking the stochastic fate of cells of the renin lineage after podocyte depletion using multicolor reporters and intravital imaging Open
Podocyte depletion plays a major role in focal segmental glomerular sclerosis (FSGS). Because cells of the renin lineage (CoRL) serve as adult podocyte and parietal epithelial cell (PEC) progenitor candidates, we generated Ren1cCre/R26R-Co…
View article: Compound effects of aging and experimental FSGS on glomerular epithelial cells
Compound effects of aging and experimental FSGS on glomerular epithelial cells Open
Advanced age portends a poorer prognosis in FSGS. To understand the impact of age on glomerular podocytes and parietal epithelial cells (PECs), experimental FSGS was induced in 3m-old mice (20-year old human age) and 27m-old mice (78-year …
View article: Reducing mTOR augments parietal epithelial cell density in a model of acute podocyte depletion and in aged kidneys
Reducing mTOR augments parietal epithelial cell density in a model of acute podocyte depletion and in aged kidneys Open
Parietal epithelial cell (PEC) response to glomerular injury may underlie a common pathway driving fibrogenesis following podocyte loss that typifies several glomerular disorders. Although the mammalian target of rapamycin (mTOR) pathway i…
View article: Partial podocyte replenishment in experimental FSGS derives from nonpodocyte sources
Partial podocyte replenishment in experimental FSGS derives from nonpodocyte sources Open
The current studies used genetic fate mapping to prove that adult podocytes can be partially replenished following depletion. Inducible NPHS2-rtTA/tetO-Cre/RS-ZsGreen-R reporter mice were generated to permanently label podocytes with the Z…
View article: Renin-Angiotensin-Aldosterone System Inhibition Increases Podocyte Derivation from Cells of Renin Lineage
Renin-Angiotensin-Aldosterone System Inhibition Increases Podocyte Derivation from Cells of Renin Lineage Open
Because adult podocytes cannot proliferate and are therefore unable to self-renew, replacement of these cells depends on stem/progenitor cells. Although podocyte number is higher after renin-angiotensin-aldosterone system (RAAS) inhibition…