Explanipedia

The asymmetric opening of HIV-1 Env by a potent CD4 mimetic enables anti-coreceptor binding site antibodies to mediate ADCC Open

Jonathan Richard, Guillaume Beaudoin-Bussières, Katrina Dionne, Zabrina C. Lang, Doreen Matthies , et al. · 2025

HIV-1 envelope glycoproteins (Env) from primary HIV-1 isolates typically adopt a pretriggered "closed" conformation that resists to CD4-induced (CD4i) non-neutralizing antibodies (nnAbs) mediating antibody-dependent cellular cytotoxicity (…

Optimization of VE607 to generate analogs with improved neutralization activities against SARS-CoV-2 variants Open

Shilei Ding, Derek Yang, Irfan Ullah, Ling Niu, Matthew Unger , et al. · 2025

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains a threat to human health, particularly among immunocompromised and elderly individuals, given their heightened vulnerability to coronavirus disease 2019 (COVID-…

Towards high-resolution in-situ structural biology of membrane protein complexes Open

Rilee Zeinert, Madolyn Britt, Elissa Moller, Fei Zhou, Alexander J. Sodt , et al. · 2025

Membrane protein structure determination is technically challenging and further complicated by the removal or displacement of lipids, which can result in complex dissociation, non-native conformations, or a strong preference for certain st…

The asymmetric opening of HIV-1 Env by a potent CD4 mimetic enables anti-coreceptor binding site antibodies to mediate ADCC Open

Jonathan Richard, Michael W. Grunst, Ling Niu, Marco A. Díaz-Salinas, William D. Tolbert , et al. · 2024

HIV-1 envelope glycoproteins (Env) from primary HIV-1 isolates typically adopt a pretriggered “closed” conformation that resists to CD4-induced (CD4i) non-neutralizing antibodies (nnAbs) mediating antibody-dependent cellular cytotoxicity (…

Magnesium Transporter MgtA revealed as a Dimeric P-type ATPase Open

Rilee Zeinert, Fei Zhou, Pedro Franco, Jonathan Zöller, Henry J. Lessen , et al. · 2024

Magnesium (Mg 2+ ) uptake systems are present in all domains of life given the vital role of this ion. Bacteria acquire Mg 2+ via conserved Mg 2+ channels and transporters. The transporters are required for growth when Mg 2+ is limiting or…

The lipid-mediated mechanism of mechanosensitive channel MscS inactivation Open

Elissa Moller, Madolyn Britt, Fei Zhou, Hyojik Yang, Andriy Anshkin , et al. · 2024

Interpretations of experimental conformations of mechanosensitive channels gated by ‘force from lipids’ become more reliable when native lipids are preserved in the structures. Escherichia coli MscS is an adaptive osmolyte release valve th…

Cryo-EM structures of human magnesium channel MRS2 reveal gating and regulatory mechanisms Open

Louis Tung Faat Lai, Jayashree Balaraman, Fei Zhou, Doreen Matthies · 2023

Magnesium ions (Mg 2+ ) play an essential role in cellular physiology. In mitochondria, protein and ATP synthesis and various metabolic pathways are directly regulated by Mg 2+ . MRS2, a magnesium channel located in the inner mitochondrial…

Lipid flipping in the omega-3 fatty-acid transporter Open

C. Nguyen, Hsiang‐Ting Lei, Louis Tung Faat Lai, Marc J. Gallenito, Xuelang Mu , et al. · 2023

Structure-function Analyses Reveal Key Molecular Determinants of HIV-1 CRF01_AE Resistance to the Entry Inhibitor Temsavir Open

Jérémie Prévost, Yaozong Chen, Fei Zhou, William D. Tolbert, Romain Gasser , et al. · 2023

Summary The HIV-1 entry inhibitor temsavir prevents CD4 from interacting with the envelope glycoprotein (Env) and blocks its conformational changes. To do this temsavir relies on the presence of a residue with small side chain at position …

Author response: The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins Open

Alexandria N. Miller, Patrick R. Houlihan, Ella Matamala, Deny Cabezas-Bratesco, Gi Young Lee , et al. · 2023

Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract The severe acute respi…

Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD Open

Yaozong Chen, Jérémie Prévost, Irfan Ullah, Hugo Grisales Romero, Véronique Lisi , et al. · 2022

Autolysosomal exocytosis of lipids protect neurons from ferroptosis Open

Isha Ralhan, Jinlan Chang, Matthew J. Moulton, Lindsey D. Goodman, Nathanael Y.J. Lee , et al. · 2022

During oxidative stress neurons release lipids that are internalized by glia. Defects in this coordinated process play an important role in several neurodegenerative diseases. Yet, the mechanisms of lipid release and its consequences on ne…

The SARS-CoV-2 accessory protein Orf3a is not an ion channel, but does interact with trafficking proteins Open

Alexandria N. Miller, Patrick R. Houlihan, Ella Matamala, Deny Cabezas-Bratesco, Gi Young Lee , et al. · 2022

The severe acute respiratory syndrome associated coronavirus 2 (SARS-CoV-2) and SARS-CoV-1 accessory protein Orf3a colocalizes with markers of the plasma membrane, endocytic pathway, and Golgi apparatus. Some reports have led to annotation…

Lipid flipping in the omega-3 fatty-acid transporter Open

C. Nguyen, Hsiang‐Ting Lei, Louis Tung Faat Lai, Marc J. Gallenito, Doreen Matthies , et al. · 2022

Mfsd2a is the primary transporter for the docosahexaenoic acid (DHA), an omega-3 fatty acid, across the blood brain barrier (BBB). Defects in Mfsd2a are linked to ailments from behavioral, learning, and motor dysfunctions to severe microce…

Unique Aggregation of Retroviral Particles Pseudotyped with the Delta Variant SARS-CoV-2 Spike Protein Open

Jennifer D. Petersen, Jianming Lu, Wendy Fitzgerald, Fei Zhou, Paul S. Blank , et al. · 2022

Individuals infected with the SARS-CoV-2 Delta variant, lineage B.1.617.2, exhibit faster initial infection with a higher viral load than prior variants, and pseudotyped viral particles bearing the SARS-CoV-2 Delta variant spike protein in…

Allosteric interactions prime androgen receptor dimerization and activation Open

Elizabeth V. Wasmuth, Arnaud Vanden Broeck, Justin LaClair, Elizabeth A. Hoover, Kayla E. Lawrence , et al. · 2022

The Delta variant SARS-CoV-2 spike protein uniquely promotes aggregation of pseudotyped viral particles Open

Jennifer D. Petersen, Jianming Lu, Wendy Fitzgerald, Fei Zhou, Paul S. Blank , et al. · 2022

Individuals infected with the SARS-CoV-2 Delta variant, lineage B.1.617.2, exhibit faster initial infection with a higher viral load than prior variants, and pseudotyped particles bearing the SARS-CoV-2 Delta variant spike protein induce a…

Allosteric interactions prime androgen receptor dimerization and activation Open

Elizabeth V. Wasmuth, Arnaud Vanden Broeck, Justin LaClair, Elizabeth A. Hoover, Kayla E. Lawrence , et al. · 2022

Summary The androgen receptor (AR) is a steroid receptor and master transcription factor that governs gene expression programs required for luminal development of prostate epithelium, formation of muscle tissue and maintenance of the male …

The Structural Basis of Rubisco Phase Separation in the Pyrenoid Open

Shan He, Hui‐Ting Chou, Doreen Matthies, Tobias Wunder, Moritz T. Meyer , et al. · 2020

Approximately one-third of global CO 2 fixation occurs in a phase separated algal organelle called the pyrenoid. Existing data suggest that the pyrenoid forms by the phase-separation of the CO 2 -fixing enzyme Rubisco with a linker protein…

Microdomains form on the luminal face of neuronal extracellular vesicle membranes Open

Doreen Matthies, Nathanael Y. J. Lee, Ian Gatera, H. Amalia Pasolli, Xiaowei Zhao , et al. · 2020

Decision letter: Structure and mechanism of the Mrp complex, an ancient cation/proton antiporter Open

Joel R. Meyerson, Doreen Matthies · 2020

Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Multiple resistance and pH adaptation (Mrp…

Transport mechanism of the neuronal excitatory amino acid transporter Open

Biao Qiu, Doreen Matthies, Eva Fortea, Zhiheng Yu, Olga Boudker · 2020

Human excitatory amino acid transporter 3 (hEAAT3) mediates glutamate uptake in neurons, intestine, and kidney. Here, we report Cryo-EM structures of hEAAT3 in several functional states where the transporter is empty, bound to coupled sodi…

Single-particle Cryo-EM of Membrane Proteins - Success Stories and Current Challenges Open

Doreen Matthies, Biao Qiu, Chanhyung Bae, Eduardo Perozo, Kenton J. Swartz , et al. · 2020

Neuron-Astrocyte Metabolic Coupling Protects against Activity-Induced Fatty Acid Toxicity Open

Maria S. Ioannou, Jesse Jackson, Shu‐Hsien Sheu, Chi‐Lun Chang, Aubrey V. Weigel , et al. · 2019

Author response: Single-particle cryo-EM structure of a voltage-activated potassium channel in lipid nanodiscs Open

Doreen Matthies, Chanhyung Bae, Gilman E. S. Toombes, Tara Fox, Alberto Bartesaghi , et al. · 2018

Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Voltage-activated potassium (Kv) channels …

CryoEM Models and Associated Data Submitted to the 2015/2016 EMDataBank Model Challenge Open

Catherine L. Lawson, Andriy Kryshtafovych, Wah Chiu, Paul D. Adams, Axel T. Brünger , et al. · 2018

Files and metadata associated with the EMDataBank/Unified Data Resource for 3DEM 2015/2016 Models Challenge hosted at challenges.emdatabank.org are deposited.\n\nAll members of the Scientific Community--at all l…

Cryoem Models And Associated Data Submitted To The 2015/2016 Emdatabank Model Challenge Open

Catherine L. Lawson, Andriy Kryshtafovych, Wah Chiu, Paul D. Adams, Axel T. Brünger , et al. · 2018

Files and metadata associated with the EMDataBank/Unified Data Resource for 3DEM 2015/2016 Models Challenge hosted at challenges.emdatabank.org are deposited. All members of the Scientific Community--at all levels of experience--were invit…

Structural basis for early-onset neurological disorders caused by mutations in human selenocysteine synthase Open

Anupama K. Puppala, Rachel L. French, Doreen Matthies, Ulrich Baxa, Sriram Subramaniam , et al. · 2016

Selenocysteine synthase (SepSecS) catalyzes the terminal reaction of selenocysteine, and is vital for human selenoproteome integrity. Autosomal recessive inheritance of mutations in SepSecS–Ala239Thr, Thr325Ser, Tyr334Cys and Tyr429*–induc…

Using Cryo-EM to Map Small Ligands on Dynamic Metabolic Enzymes: Studies with Glutamate Dehydrogenase Open

Mario J. Borgnia, Soojay Banerjee, Alan Merk, Doreen Matthies, Alberto Bartesaghi , et al. · 2016

The crystal structure of human GlnRS provides basis for the development of neurological disorders Open

Jana Ognjenović, Jiang Wu, Doreen Matthies, Ulrich Baxa, Sriram Subramaniam , et al. · 2016

Cytosolic glutaminyl-tRNA synthetase (GlnRS) is the singular enzyme responsible for translation of glutamine codons. Compound heterozygous mutations in GlnRS cause severe brain disorders by a poorly understood mechanism. Herein, we present…

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