Doris Ribitsch
YOU?
Author Swipe
View article: Tuning casein coagulation and structure: Exploring the effects of ionic strength and glycerol
Tuning casein coagulation and structure: Exploring the effects of ionic strength and glycerol Open
Casein-based materials offer a sustainable platform for advanced biomaterials, yet the impact of ionic strength on their macroscopic structure and properties remains largely unexplored. This study investigates the coagulation (and gelation…
View article: Phage display screening for highly specific nickel- and cobalt-binding peptides for bio-recovery of metals
Phage display screening for highly specific nickel- and cobalt-binding peptides for bio-recovery of metals Open
Electronic waste is a valuable source of critical metals like nickel and cobalt, but their recovery is challenging. Current recycling processes use harsh conditions and toxic chemicals, which is why environmentally friendly alternatives ar…
View article: Unveiling potential of Gordonia species and their cutinases for polyester decomposition
Unveiling potential of Gordonia species and their cutinases for polyester decomposition Open
The growing issue of petroleum-based polymer waste demands sustainable recycling strategies, with enzymatic processes offering a promising solution. This study investigates enzymatic decomposition of polyethylene terephthalate (PET) and po…
View article: The quest for the new CaLB: potential of three thermostable polyester hydrolases for esterification reactions
The quest for the new CaLB: potential of three thermostable polyester hydrolases for esterification reactions Open
The potential of three thermophilic enzymes from the α,β-hydrolases superfamily, that have recently been described for polyester hydrolysis, was investigated for polyester synthesis, esterification and transesterification reactions. The hy…
View article: Depolymerisation of waste- and bio-based polyesters by an activated sludge hydrolase
Depolymerisation of waste- and bio-based polyesters by an activated sludge hydrolase Open
This study investigates enzymatic hydrolysis of four novel polyesters (REPolymers) with different structures based on terephthalic acid, ethylene glycol, glutaric acid, and decanediol. These polyesters were synthesized from PET building bl…
View article: Ions and low molecular weight components in sulfite pulp mills strongly reduce or boost lipases activity on lipophilic wood extractives
Ions and low molecular weight components in sulfite pulp mills strongly reduce or boost lipases activity on lipophilic wood extractives Open
Lipophilic wood extractives (LWEs), including triterpenes, fatty acids, resin acids, waxes, alcohols, sterols, sterol esters, and glycerides, pose significant challenges for pulp and paper mills. Microbial triacylglycerol lipases (EC 3.1.1…
View article: Enhanced Adsorption and Enzymatic Hydrolysis of Polyethylene Terephthalate by Cutinase with an N-Terminal Hydrophobic Tether
Enhanced Adsorption and Enzymatic Hydrolysis of Polyethylene Terephthalate by Cutinase with an N-Terminal Hydrophobic Tether Open
The environmental challenges presented by plastic waste, particularly polyethylene terephthalate (PET), necessitates innovative biodegradation strategies. The cutinase from Thermobifida cellulosilytica, Thc_Cut1 (Cut), was site-specificall…
View article: Screening assay for polyester hydrolyzing microorganisms using fluorescence-labeled poly(butylene adipate)
Screening assay for polyester hydrolyzing microorganisms using fluorescence-labeled poly(butylene adipate) Open
Despite recent advances, there is still a demand for more efficient enzymes hydrolyzing synthetic polymers. Automated high throughput screening strategies of microorganisms from different environments could yield novel enzymes but require …
View article: Activity of an anaerobic Thermoanaerobacterales hydrolase on aliphatic and aromatic polyesters
Activity of an anaerobic Thermoanaerobacterales hydrolase on aliphatic and aromatic polyesters Open
This study focuses on the biochemical characterization of a new hydrolase (Thb) expressed from anaerobic Thermoanaerobacterales, which could be used to improve biogas plant efficiency for plastic waste treatment. The specificity of Thb for…
View article: Detoxification of aflatoxin B1 by a Bacillus subtilis spore coat protein through formation of the main metabolites AFQ1 and epi-AFQ1
Detoxification of aflatoxin B1 by a Bacillus subtilis spore coat protein through formation of the main metabolites AFQ1 and epi-AFQ1 Open
A variety of important agricultural crops host fungi from the Aspergillus genus can produce cancerogenic secondary metabolites such as aflatoxins. Consequently, novel strategies for detoxification and their removal from food and feed chain…
View article: Approaches to Control and Monitor Protease Levels in Chronic Wounds
Approaches to Control and Monitor Protease Levels in Chronic Wounds Open
Some wounds do not follow the typical healing trajectory and show very little, if any, progress. They dreadfully threaten the patients' quality of life and may even lead to limb amputation and death. Several interrelated systemic and local…
View article: Microplastics in the Alpine watercycle – A combination of methods to cover the widest possible size range 
Microplastics in the Alpine watercycle – A combination of methods to cover the widest possible size range  Open
Plastic waste as a permanent pollutant in the environment is of increasing concern due to its largely unknown long-term effects on biota. The occurrence in rivers, has, compared to research in the oceans, only become the focus of scientifi…
View article: Multifunctional Casein-Based Wound Dressing Capable of Monitoring and Moderating the Proteolytic Activity of Chronic Wounds
Multifunctional Casein-Based Wound Dressing Capable of Monitoring and Moderating the Proteolytic Activity of Chronic Wounds Open
Every 1.2 s, a diabetic foot ulcer is developed, and every 20 s, one amputation is carried out in diabetic patients. Monitoring and controlling protease activity have been considered as a strategy for more efficient management of diabetic …
View article: Optimising chemo-enzymatic separation of polyester cellulose blends
Optimising chemo-enzymatic separation of polyester cellulose blends Open
The study demonstrates the potential of enzymatic hydrolysis and alkaline pretreatment for sustainable blended textile recycling. The target is complete cellulose removal while preserving the polyester integrity for recovery. Interactions …
View article: Laccase-mediated degradation of petroleum hydrocarbons in historically contaminated soil
Laccase-mediated degradation of petroleum hydrocarbons in historically contaminated soil Open
Laccases (EC1.10.3.2) have attracted growing attention in bioremediation research due to their high reactivity and substrate versatility. In this study, three genes for potential novel laccases were identified in an enrichment culture from…
View article: Eight Up-Coming Biotech Tools to Combat Climate Crisis
Eight Up-Coming Biotech Tools to Combat Climate Crisis Open
Biotechnology has a high potential to substantially contribute to a low-carbon society. Several green processes are already well established, utilizing the unique capacity of living cells or their instruments. Beyond that, the authors beli…
View article: Dihydropyrimidinase from Saccharomyces kluyveri can hydrolyse polyamides
Dihydropyrimidinase from Saccharomyces kluyveri can hydrolyse polyamides Open
In Saccharomyces kluyveri, dihydropyrimidinase (DHPaseSK) is involved in the pyrimidine degradation pathway, which includes the reversible ring cleavage between nitrogen 3 and carbon 4 of 5,6-dihydrouracil. In this study, DPHaseSK was succ…
View article: Bio-upcycling of multilayer materials and blends: closing the plastics loop
Bio-upcycling of multilayer materials and blends: closing the plastics loop Open
The urge to discover and develop new technologies for closing the plastic carbon cycle is motivating industries, governments, and academia to work closely together to find suitable solutions in a timely manner. In this review article, a co…
View article: ENZYME BASED RECYCLING PROCESSES
ENZYME BASED RECYCLING PROCESSES Open
Novel strategies allowing environmentally friendly recycling of plastics are strongly needed. Enzymes have shown high potential, especially for the recovery of building blocks from multilayer materials which will be discussed in this paper…
View article: Effect of Binding Modules Fused to Cutinase on the Enzymatic Synthesis of Polyesters
Effect of Binding Modules Fused to Cutinase on the Enzymatic Synthesis of Polyesters Open
In relation to the development of environmentally-friendly processing technologies for the continuously growing market of plastics, enzymes play an important role as green and sustainable biocatalysts. The present study reports the use of …
View article: Residue-Specific Incorporation of the Non-Canonical Amino Acid Norleucine Improves Lipase Activity on Synthetic Polyesters
Residue-Specific Incorporation of the Non-Canonical Amino Acid Norleucine Improves Lipase Activity on Synthetic Polyesters Open
Environmentally friendly functionalization and recycling processes for synthetic polymers have recently gained momentum, and enzymes play a central role in these procedures. However, natural enzymes must be engineered to accept synthetic p…
View article: Oxidation of Various Kraft Lignins with a Bacterial Laccase Enzyme
Oxidation of Various Kraft Lignins with a Bacterial Laccase Enzyme Open
Modification of kraft lignin (KL), traditionally uses harsh and energy-demanding physical and chemical processes. In this study, the potential of the bacterial laccase CotA (spore coating protein A) for oxidation of KL under mild condition…
View article: Structure‐function analysis of two closely related cutinases from <i>Thermobifida cellulosilytica</i>
Structure‐function analysis of two closely related cutinases from <i>Thermobifida cellulosilytica</i> Open
Cutinases can play a significant role in a biotechnology‐based circular economy. However, relatively little is known about the structure–function relationship of these enzymes, knowledge that is vital to advance optimized, engineered enzym…
View article: Comparison of a fungal and a bacterial laccase for lignosulfonate polymerization
Comparison of a fungal and a bacterial laccase for lignosulfonate polymerization Open
Lignin is the second most abundant biopolymer on earth and accrues in large amounts in the pulp- and paper industry. While currently lignins are mainly used for low-value applications such as energy production, recently the potential of la…
View article: A Simple and Straightforward Method for Activity Measurement of Carbonic Anhydrases
A Simple and Straightforward Method for Activity Measurement of Carbonic Anhydrases Open
Carbonic anhydrase (CA) is an enzyme of high interest due to its high implications relative to the medical and environmental sectors. In the current paper, an enzyme assay for the determination of CA activity is proposed and it is characte…
View article: Together Is Better: The Rumen Microbial Community as Biological Toolbox for Degradation of Synthetic Polyesters
Together Is Better: The Rumen Microbial Community as Biological Toolbox for Degradation of Synthetic Polyesters Open
Graphical Abstract Identfication of plastics degradation and microbial community analysis of Rumen.