Duolong Zhu
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View article: Synthetic microbial co-cultures for modular bioelectronic sensing in diverse environments
Synthetic microbial co-cultures for modular bioelectronic sensing in diverse environments Open
Human disruption of ecosystems poses a significant threat to global health, driving the need for low-cost, low-power, and easily deployable sensors for environmental and health monitoring. Microbial bioelectronic sensors are particularly w…
View article: Microbial Biosensor for Sensing and Treatment of Intestinal Inflammation
Microbial Biosensor for Sensing and Treatment of Intestinal Inflammation Open
Synthetic biology has enabled the development of biosensors to detect intestinal inflammation, yet few target the clinically validated biomarker of intestinal inflammation calprotectin with both diagnostic and therapeutic capabilities. Her…
View article: Control of <i>Clostridioides difficile</i> virulence and physiology by the flagellin homeostasis checkpoint FliC-FliW-CsrA in the absence of motility
Control of <i>Clostridioides difficile</i> virulence and physiology by the flagellin homeostasis checkpoint FliC-FliW-CsrA in the absence of motility Open
Mutations affecting Clostridioides difficile flagellin (FliC) have been shown to be hypervirulent in animal models and display increased toxin production and alterations in central metabolism. The regulation of flagellin levels in bacteria…
View article: Genomic and phenotypic characterization of a Clostridioides difficile strain of the epidemic ST37 type from China
Genomic and phenotypic characterization of a Clostridioides difficile strain of the epidemic ST37 type from China Open
Clostridioides difficile strains of sequence type (ST) 37, primarily including PCR ribotype (RT) 017, are prevalent in mainland China. Our study aimed to compare the major virulence factors of an epidemic C. difficile isolate of ST37 type …
View article: Microbial biosensor for sensing and treatment of intestinal inflammation
Microbial biosensor for sensing and treatment of intestinal inflammation Open
Substantial synthetic biology efforts have been made to engineer biosensors to detect intestinal inflammation, however none target the most clinically accepted biomarker, calprotectin. To develop an in situ biosensor for calprotectin, we o…
View article: A bioengineered probiotic for the oral delivery of a peptide Kv1.3 channel blocker to treat rheumatoid arthritis
A bioengineered probiotic for the oral delivery of a peptide Kv1.3 channel blocker to treat rheumatoid arthritis Open
Engineered microbes for the delivery of biologics are a promising avenue for the treatment of various conditions such as chronic inflammatory disorders and metabolic disease. In this study, we developed a genetically engineered probiotic d…
View article: Control of<i>Clostridiodes difficile</i>virulence and physiology by the flagellin homeostasis checkpoint FliC-FliW-CsrA in the absence of motility
Control of<i>Clostridiodes difficile</i>virulence and physiology by the flagellin homeostasis checkpoint FliC-FliW-CsrA in the absence of motility Open
Mutations affecting Clostridioides difficile flagellin (FliC) have been shown to be hypervirulent in animal models and display increased toxin production and alterations in central metabolism. The regulation of flagellin levels in bacteria…
View article: A bioengineered probiotic for the oral delivery of a peptide Kv1.3 channel blocker to treat rheumatoid arthritis
A bioengineered probiotic for the oral delivery of a peptide Kv1.3 channel blocker to treat rheumatoid arthritis Open
Engineered microbes for the delivery of biologics is a promising avenue for the treatment of various conditions such as chronic inflammatory disorders and metabolic disease. In this study, we developed a genetically engineered probiotic de…
View article: Development of an Effective Nontoxigenic Clostridioides difficile–Based Oral Vaccine against C. difficile Infection
Development of an Effective Nontoxigenic Clostridioides difficile–Based Oral Vaccine against C. difficile Infection Open
Clostridioides difficile is an enteric pathogen, and symptoms of C. difficile infection (CDI) are mainly by two exotoxins TcdA and TcdB. Active vaccination is cost-effective approach to prevent CDI and high rates of recurrence.
View article: FliW and CsrA Govern Flagellin (FliC) Synthesis and Play Pleiotropic Roles in Virulence and Physiology of Clostridioides difficile R20291
FliW and CsrA Govern Flagellin (FliC) Synthesis and Play Pleiotropic Roles in Virulence and Physiology of Clostridioides difficile R20291 Open
Clostridioides difficile flagellin FliC is associated with toxin gene expression, bacterial colonization, and virulence, and is also involved in pleiotropic gene regulation during in vivo infection. However, how fliC expression is regulate…
View article: FliW and CsrA govern flagellin (FliC) synthesis and play pleiotropic roles in virulence and physiology of<i>Clostridioides difficile</i>R20291
FliW and CsrA govern flagellin (FliC) synthesis and play pleiotropic roles in virulence and physiology of<i>Clostridioides difficile</i>R20291 Open
Clostridioides difficile is a Gram-positive, spore-forming, and toxin-producing anaerobe that can cause nosocomial antibiotic-associated intestinal disease. In C. difficile , the expression of flagellar genes is coupled to toxin gene regul…
View article: Cwl0971, a novel peptidoglycan hydrolase, plays pleiotropic roles in <i>Clostridioides difficile</i> R20291
Cwl0971, a novel peptidoglycan hydrolase, plays pleiotropic roles in <i>Clostridioides difficile</i> R20291 Open
Summary Clostridioides difficile is a Gram‐positive, spore‐forming, toxin‐producing anaerobe that can cause nosocomial antibiotic‐associated intestinal disease. Although the production of toxin A (TcdA) and toxin B (TcdB) contribute to the…
View article: Cwl0971, a novel peptidoglycan hydrolase, plays pleiotropic roles in<i>Clostridioides difficile</i>R20291
Cwl0971, a novel peptidoglycan hydrolase, plays pleiotropic roles in<i>Clostridioides difficile</i>R20291 Open
Summary Clostridioides difficile is a Gram-positive, spore-forming, toxin-producing anaerobe that can cause nosocomial antibiotic-associated intestinal disease. Although the production of toxin A (TcdA) and toxin B (TcdB) contribute to the…
View article: Cwp22, a novel peptidoglycan cross‐linking enzyme, plays pleiotropic roles in <i>Clostridioides difficile</i>
Cwp22, a novel peptidoglycan cross‐linking enzyme, plays pleiotropic roles in <i>Clostridioides difficile</i> Open
Summary Clostridioides difficile is a Gram‐positive, spore‐forming, toxin‐producing anaerobe pathogen, and can induce nosocomial antibiotic‐associated intestinal disease. While production of toxin A (TcdA) and toxin B (TcdB) contribute to …
View article: A Novel Bacteriophage Lysin-Human Defensin Fusion Protein Is Effective in Treatment of Clostridioides difficile Infection in Mice
A Novel Bacteriophage Lysin-Human Defensin Fusion Protein Is Effective in Treatment of Clostridioides difficile Infection in Mice Open
Clostridioides difficile is the leading cause of worldwide antibiotics-associated diarrhea. In this study, we report the construction and evaluation of a novel bacteriophage lysin-human defensin fusion protein targeting C. difficile. The f…
View article: MOESM3 of Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system
MOESM3 of Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system Open
Additional file 3: Data S1A. Genome sequencing and analysis of L. lactis 9k-5A. (A) Genome sequencing of L. lactis 9k-5A.
View article: MOESM5 of Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system
MOESM5 of Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system Open
Additional file 5: Data S1B. Genome sequencing and analysis of L. lactis 9k-5A. (B) Arrangement of 66 large scaffolds in L. lactis 9k-5A.
View article: MOESM10 of Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system
MOESM10 of Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system Open
Additional file 10: Table S3. List of 93 DEGs in mutants.
View article: MOESM8 of Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system
MOESM8 of Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system Open
Additional file 8: Table S2. Results of transcriptome analysis.
View article: MOESM11 of Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system
MOESM11 of Restructured Lactococcus lactis strains with emergent properties constructed by a novel highly efficient screening system Open
Additional file 11: Data S3A. Results of significant enrichment of KEGG pathway in L. lactis 9k-5A. (A) Transcriptome analysis of 93 DEGs in mutants.
View article: Characterization of the virulence of a non-RT027, non-RT078 and binary toxin-positive <i>Clostridium difficile</i> strain associated with severe diarrhea
Characterization of the virulence of a non-RT027, non-RT078 and binary toxin-positive <i>Clostridium difficile</i> strain associated with severe diarrhea Open
The expression of the Clostridium difficile binary toxin CDT is generally observed in the RT027 (ST1) and RT078 (ST11) C. difficile isolates, which are associated with severe C. difficile infection (CDI). However, we recently reported that…
View article: Bioprospecting Deep-Sea Actinobacteria for Novel Anti-infective Natural Products
Bioprospecting Deep-Sea Actinobacteria for Novel Anti-infective Natural Products Open
The global prevalence of drug resistance has created an urgent need for the discovery of novel anti-infective drugs. The major source of antibiotics in current clinical practice is terrestrial actinobacteria; the less-exploited deep-sea ac…
View article: Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk
Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk Open
Nisin, an important bacteriocin from Lactococcus lactis subsp., is primarily active against various Gram-positive bacteria. Leucocin C, produced by Leuconostoc carnosum 4010, is a class IIa bacteriocin used to inhibit the growth of Listeri…
View article: Clostridioides difficile Biology: Sporulation, Germination, and Corresponding Therapies for C. difficile Infection
Clostridioides difficile Biology: Sporulation, Germination, and Corresponding Therapies for C. difficile Infection Open
Clostridioides difficile is a Gram-positive, spore-forming, toxin-producing anaerobe, and an important nosocomial pathogen. Due to the strictly anaerobic nature of the vegetative form, spores are the main morphotype of infection and transm…
View article: Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000
Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000 Open
The genome-streamlined L. lactis 9 k-4 outcompeted the parental strain in several physiological traits assessed. Moreover, L. lactis 9 k-4 exhibited good properties as platform organism for protein production. In future works, the genome o…
View article: Isolation of strong constitutive promoters from<i>Lactococcus lactis</i>subsp.<i>lactis</i>N8
Isolation of strong constitutive promoters from<i>Lactococcus lactis</i>subsp.<i>lactis</i>N8 Open
The synthesis of heterologous proteins in Lactococcus lactis is strongly influenced by the promoter selected for the expression. The nisin A promoter is commonly used for induced expression of proteins in L. lactis, whereas few constitutiv…