Dylan G. Ryan
YOU?
Author Swipe
View article: An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice
An inherited mitochondrial DNA mutation remodels inflammatory cytokine responses in macrophages and in vivo in mice Open
Impaired mitochondrial bioenergetics in macrophages promotes hyperinflammatory cytokine responses, but whether inherited mtDNA mutations drive similar phenotypes is unknown. Here, we profiled macrophages harbouring a heteroplasmic mitochon…
View article: Chitinase-1 inhibition attenuates metabolic dysregulation and restores homeostasis in MASH animal models
Chitinase-1 inhibition attenuates metabolic dysregulation and restores homeostasis in MASH animal models Open
Background OATD-01 is a chitinase-1 (CHIT1) inhibitor, reducing inflammation and fibrosis in animal models where chronic inflammation leads to tissue remodeling. CHIT1, predominantly secreted by macrophages, is overexpressed in metabolic d…
View article: Clinically translatable mitochondrial gene therapy in muscle using tandem mtZFN architecture
Clinically translatable mitochondrial gene therapy in muscle using tandem mtZFN architecture Open
Mutations in the mitochondrial genome (mtDNA) often lead to clinical pathologies. Mitochondrially-targeted zinc finger nucleases (mtZFNs) have been successful in reducing the levels of mutation-bearing mtDNA both in vivo and in vitro, resu…
View article: Mitochondrial respiratory complex III sustains IL-10 production in activated macrophages and promotes tumor-mediated immune evasion
Mitochondrial respiratory complex III sustains IL-10 production in activated macrophages and promotes tumor-mediated immune evasion Open
The cytokine interleukin-10 (IL-10) limits the immune response and promotes resolution of acute inflammation. Because of its immunosuppressive effects, IL-10 up-regulation is a common feature of tumor progression and metastasis. Recently, …
View article: An inherited mtDNA mutation remodels inflammatory cytokine responses in macrophages and <i>in vivo</i>
An inherited mtDNA mutation remodels inflammatory cytokine responses in macrophages and <i>in vivo</i> Open
Impaired mitochondrial bioenergetics in macrophages can drive hyperinflammatory cytokine responses 1–6 , but whether this may also be caused by inherited mtDNA mutations is unknown. Here, we address this question using a multi-omic approac…
View article: Chitinase-1 inhibition reverses metabolic dysregulation and restores homeostasis in MASH animal models
Chitinase-1 inhibition reverses metabolic dysregulation and restores homeostasis in MASH animal models Open
OATD-01 is a chitinase-1 (CHIT1) inhibitor, reducing inflammation and fibrosis in animal models where chronic inflammation leads to tissue remodeling. CHIT1, predominantly secreted by macrophages, is overexpressed in metabolic-dysfunction-…
View article: High-dimensional single-cell analysis of human natural killer cell heterogeneity
High-dimensional single-cell analysis of human natural killer cell heterogeneity Open
Natural killer (NK) cells are innate lymphoid cells (ILCs) contributing to immune responses to microbes and tumors. Historically, their classification hinged on a limited array of surface protein markers. Here, we used single-cell RNA sequ…
View article: Associations between TCA cycle plasma metabolites and fatigue in black females with systemic lupus erythematosus: An untargeted metabolomics pilot study
Associations between TCA cycle plasma metabolites and fatigue in black females with systemic lupus erythematosus: An untargeted metabolomics pilot study Open
Objective In this pilot study, we used untargeted metabolomics to identify biochemical mechanisms or biomarkers potentially underlying SLE-related fatigue. Methods Metabolon conducted untargeted metabolomic plasma profiling using ultrahigh…
View article: Multifaceted mitochondria in innate immunity
Multifaceted mitochondria in innate immunity Open
The ability of mitochondria to transform the energy we obtain from food into cell phosphorylation potential has long been appreciated. However, recent decades have seen an evolution in our understanding of mitochondria, highlighting their …
View article: High-Dimensional Single-Cell Analysis of Natural Killer Cell Heterogeneity in Human Blood
High-Dimensional Single-Cell Analysis of Natural Killer Cell Heterogeneity in Human Blood Open
Natural killer (NK) cells are innate lymphoid cells (ILCs) that serve as a first line of defense against pathogens and tumors. Historically, their classification hinged on a limited array of surface protein markers. However, recent advance…
View article: Inflammatory mitochondrial nucleic acids as drivers of pathophysiology
Inflammatory mitochondrial nucleic acids as drivers of pathophysiology Open
Mitochondria are intracellular membrane-bound organelles that harness the chemical energy in food to drive adenosine triphosphate (ATP) synthesis via oxidative phosphorylation. In the last several decades, mitochondria have emerged as cent…
View article: Basic Mechanisms of Immunometabolites in Shaping the Immune Response
Basic Mechanisms of Immunometabolites in Shaping the Immune Response Open
Background: Innate immune cells play a crucial role in responding to microbial infections, but their improper activation can also drive inflammatory disease. For this reason, their activation state is governed by a multitude of factors, in…
View article: Massive hemoptysis bridged with VV ECMO: A case report
Massive hemoptysis bridged with VV ECMO: A case report Open
Objective Extracorporeal membrane oxygenation (ECMO) can provide full pulmonary support when a patient is completely apneic. The combination of veno-venous (VV) ECMO and induced apnea can be utilized to control significant hemoptysis. We p…
View article: Macrophage innate training induced by IL-4 and IL-13 activation enhances OXPHOS driven anti-mycobacterial responses
Macrophage innate training induced by IL-4 and IL-13 activation enhances OXPHOS driven anti-mycobacterial responses Open
Macrophages are a highly adaptive population of innate immune cells. Polarization with IFNγ and LPS into the ‘classically activated’ M1 macrophage enhances pro-inflammatory and microbicidal responses, important for eradicating bacteria suc…
View article: Nrf2 activation reprograms macrophage intermediary metabolism and suppresses the type I interferon response
Nrf2 activation reprograms macrophage intermediary metabolism and suppresses the type I interferon response Open
To overcome oxidative, inflammatory, and metabolic stress, cells have evolved cytoprotective protein networks controlled by nuclear factor-erythroid 2 p45-related factor 2 (Nrf2) and its negative regulator, Kelch-like ECH associated protei…
View article: Macrophage Innate Training Induced by IL-4 Activation Enhances OXPHOS Driven Anti-Mycobacterial Responses
Macrophage Innate Training Induced by IL-4 Activation Enhances OXPHOS Driven Anti-Mycobacterial Responses Open
This is the dataset pertaining to the article "Macrophage Innate Training Induced by IL-4 Activation Enhances OXPHOS Driven Anti-Mycobacterial Responses". To interpret and follow the data, please refer to the linked article, as each excel …
View article: Macrophage Innate Training Induced by IL-4 and IL-13 Activation Enhances OXPHOS Driven Anti-Mycobacterial Responses
Macrophage Innate Training Induced by IL-4 and IL-13 Activation Enhances OXPHOS Driven Anti-Mycobacterial Responses Open
Macrophages are a highly adaptive population of innate immune cells. Polarization with IFNγ and LPS into the “classically activated” M1 macrophage enhances pro-inflammatory and microbicidal responses, important for eradicating bacteria suc…
View article: IL-10-Mediated Refueling of Exhausted T Cell Mitochondria Boosts Anti-Tumour Immunity
IL-10-Mediated Refueling of Exhausted T Cell Mitochondria Boosts Anti-Tumour Immunity Open
Immunotherapy has underscored a revolution in cancer treatment. Yet, many patients fail to respond due to T cell exhaustion. Here, an intervention that restores mitochondrial function reversed the exhausted T cell phenotype to promote cyto…
View article: Nitrogen partitioning between branched-chain amino acids and urea cycle enzymes sustains renal cancer progression
Nitrogen partitioning between branched-chain amino acids and urea cycle enzymes sustains renal cancer progression Open
SUMMARY Metabolic reprogramming is critical for tumor initiation and progression. However, the exact impact of specific metabolic changes on cancer progression is poorly understood. Here, we combined multi-omics datasets of primary and met…