Caitlin E. Hatton
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View article: Spitrobot-2 advances time-resolved cryo-trapping crystallography to under 25 ms
Spitrobot-2 advances time-resolved cryo-trapping crystallography to under 25 ms Open
View article: Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography
Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography Open
View article: Apo‐state structure of the metabotropic glutamate receptor 5 transmembrane domain obtained using a photoswitchable ligand
Apo‐state structure of the metabotropic glutamate receptor 5 transmembrane domain obtained using a photoswitchable ligand Open
Metabotropic glutamate receptor 5 (mGlu5) is implicated in various neurodegenerative disorders, making it an attractive drug target. Although several ligand‐bound crystal structures of mGlu5 exist, their apo‐state crystal structure remains…
View article: SPITROBOT-2 advances time-resolved cryo-trapping crystallography to under 25 ms
SPITROBOT-2 advances time-resolved cryo-trapping crystallography to under 25 ms Open
We previously introduced the SPITROBOT, a protein crystal plunging system that enables reaction quenching via cryo-trapping with a time resolution in the millisecond range. Here we present the next generation, SPITROBOT-2, as an integrated…
View article: A mutation at the dimer interface regulates enzyme catalysis
A mutation at the dimer interface regulates enzyme catalysis Open
Fluoroacetate Dehalogenase (FAcD), a homodimeric dehalogenase of interest in bioremediation, displays half-of-the-sites reactivity, requiring allosteric communication between the subunits to coordinate the reaction. Dimer interfaces are an…
View article: Crystal structure of a bacterial photoactivated adenylate cyclase determined by serial femtosecond and serial synchrotron crystallography
Crystal structure of a bacterial photoactivated adenylate cyclase determined by serial femtosecond and serial synchrotron crystallography Open
OaPAC is a recently discovered blue-light-using flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses adenosine triphosphate and translates the light signal into the …
View article: Exploring the dynamics of allostery through multi-dimensional crystallography
Exploring the dynamics of allostery through multi-dimensional crystallography Open
View article: Crystal structure of a bacterial photoactivated adenylate cyclase determined at room temperature by serial femtosecond crystallography
Crystal structure of a bacterial photoactivated adenylate cyclase determined at room temperature by serial femtosecond crystallography Open
OaPAC is a recently discovered b lue-light u sing flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses adenosine triphosphate and translates the light signal into th…
View article: Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography
Millisecond cryo-trapping by the spitrobot crystal plunger simplifies time-resolved crystallography Open
We introduce the spitrobot , a protein crystal plunger, enabling reaction quenching via cryo-trapping with a time-resolution in the millisecond range. Protein crystals are mounted on canonical micromeshes on an electropneumatic piston, whe…
View article: Millisecond cryo-trapping by the <i>spitrobot</i> crystal plunger simplifies time-resolved crystallography
Millisecond cryo-trapping by the <i>spitrobot</i> crystal plunger simplifies time-resolved crystallography Open
We introduce the spitrobot , a protein crystal plunger, enabling reaction quenching via cryo-trapping with millisecond time-resolution. Canonical micromesh loops are mounted on an electropneumatic piston, reactions are initiated via the li…
View article: Structure of cytosine transport protein CodB provides insight into nucleobase‐cation symporter 1 mechanism
Structure of cytosine transport protein CodB provides insight into nucleobase‐cation symporter 1 mechanism Open
View article: Structure and mechanism of a cytosine transport protein
Structure and mechanism of a cytosine transport protein Open
CodB is a cytosine transporter from the Nucleobase-Cation-Symport-1 (NCS1) transporter family, a member of the widespread LeuT superfamily. Previous experiments with the nosocomial pathogen Pseudomonas aeruginosa have shown CodB also to be…
View article: Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography
Probing the modulation of enzyme kinetics by multi-temperature, time-resolved serial crystallography Open
We present an environmental enclosure for fixed-target serial crystallography, enabling X-ray diffraction experiments in a temperature window from below 10 °C to above 70 °C - a universal parameter of protein function. Via 5D-SSX time-reso…
View article: Surface-tethered planar membranes containing the β-barrel assembly machinery: a platform for investigating bacterial outer membrane protein folding
Surface-tethered planar membranes containing the β-barrel assembly machinery: a platform for investigating bacterial outer membrane protein folding Open
View article: Total chemical synthesis of lassomycin and lassomycin-amide
Total chemical synthesis of lassomycin and lassomycin-amide Open
Herein we report a practical synthetic route to the lasso peptide lassomycin (1) and C-terminal variant lassomycin-amide (2).