Robert E. Campbell
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View article: Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane–Membrane Contact Sites
Bipartite Genetically Encoded Biosensors to Sense Calcium Ion Dynamics at Membrane–Membrane Contact Sites Open
Self-complementing bipartite fluorescent proteins (FPs) are useful tools for the detection of protein-protein proximity and for localizing fluorophores to membrane-membrane contact sites. Here, we report versions of circularly permuted gre…
View article: A sensitive orange fluorescent calcium ion indicator for imaging neural activity
A sensitive orange fluorescent calcium ion indicator for imaging neural activity Open
Genetically encoded calcium indicators (GECIs) are vital tools for fluorescence-based visualization of neuronal activity with high spatial and temporal resolution. However, current highest-performance GECIs are predominantly green or red f…
View article: A toolbox for ablating excitatory and inhibitory synapses
A toolbox for ablating excitatory and inhibitory synapses Open
Recombinant optogenetic and chemogenetic proteins are potent tools for manipulating neuronal activity and controlling neural circuit function. However, there are few analogous tools for manipulating the structure of neural circuits. Here, …
View article: High-performance genetically-encoded green and red fluorescent biosensors for pyruvate
High-performance genetically-encoded green and red fluorescent biosensors for pyruvate Open
Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F 0 > 10) single fluorescent protein (FP)-based biosensors has hindered efforts to i…
View article: Synthesis and application of a photocaged-l-lactate for studying the biological roles of l-lactate
Synthesis and application of a photocaged-l-lactate for studying the biological roles of l-lactate Open
L-Lactate, once considered a metabolic waste product of glycolysis, is now recognized as a vitally important metabolite and signaling molecule in multiple biological pathways. However, exploring L-lactate's emerging intra- and extra-cellul…
View article: Comparison of peracetic acid and bacteriophage application by vascular rinsing on <i>Salmonella</i> reduction in lymph nodes of goat carcasses
Comparison of peracetic acid and bacteriophage application by vascular rinsing on <i>Salmonella</i> reduction in lymph nodes of goat carcasses Open
The ability of carcass vascular rinsing supplemented with bacteriophage (BP) and peracetic acid (PAA) to reduce Salmonella in lymph nodes (LNs) from experimentally infected goats was determined. Cull dairy goats ( n = 60) were randomly ass…
View article: A toolbox for ablating excitatory and inhibitory synapses
A toolbox for ablating excitatory and inhibitory synapses Open
Recombinant optogenetic and chemogenetic proteins are potent tools for manipulating neuronal activity and controlling neural circuit function. However, there are few analogous tools for manipulating the structure of neural circuits. Here, …
View article: Astrocyte Kir4.1 expression level territorially controls excitatory transmission in the brain
Astrocyte Kir4.1 expression level territorially controls excitatory transmission in the brain Open
Intense brain activity elevates extracellular potassium, potentially leading to overexcitation and seizures. Astrocytes are crucial for restoring healthy potassium levels, and an emerging focus on their Kir4.1 channels has reopened the que…
View article: PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities
PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities Open
Genetically encoded calcium (Ca 2+ ) indicators (GECIs) are widely used for imaging neuronal activity, yet current limitations of existing red fluorescent GECIs have constrained their applicability. The inherently dim fluorescence and low …
View article: A toolbox for ablating excitatory and inhibitory synapses
A toolbox for ablating excitatory and inhibitory synapses Open
Recombinant optogenetic and chemogenetic proteins are potent tools for manipulating neuronal activity and controlling neural circuit function. However, there are few analogous tools for manipulating the structure of neural circuits. Here, …
View article: A toolbox for ablating excitatory and inhibitory synapses
A toolbox for ablating excitatory and inhibitory synapses Open
Recombinant optogenetic and chemogenetic proteins that manipulate neuronal activity are potent tools for activating and inhibiting neuronal circuit function. However, there are few analogous tools for manipulating the structure of neural c…
View article: A chemigenetic indicator based on a synthetic chelator and a green fluorescent protein for imaging of intracellular sodium ions
A chemigenetic indicator based on a synthetic chelator and a green fluorescent protein for imaging of intracellular sodium ions Open
We report the development of a chemigenetic fluorescent sodium ion indicator with an affinity optimized for imaging physiologically relevant sodium ion concentrations in mammalian cells.
View article: High‐Throughput Discovery of Substrate Peptide Sequences for E3 Ubiquitin Ligases Using a cDNA Display Method
High‐Throughput Discovery of Substrate Peptide Sequences for E3 Ubiquitin Ligases Using a cDNA Display Method Open
Cells utilize ubiquitin as a posttranslational protein modifier to convey various signals such as proteasomal degradation. The dysfunction of ubiquitylation or following proteasomal degradation can give rise to the accumulation and aggrega…
View article: A toolbox for ablating excitatory and inhibitory synapses
A toolbox for ablating excitatory and inhibitory synapses Open
Recombinant optogenetic and chemogenetic proteins are potent tools for manipulating neuronal activity and controlling neural circuit function. However, there are few analogous tools for manipulating the structure of neural circuits. Here, …
View article: A red fluorescent genetically encoded biosensor for in vivo imaging of extracellular L-lactate dynamics
A red fluorescent genetically encoded biosensor for in vivo imaging of extracellular L-lactate dynamics Open
The transient build-up of L-lactate in tissue is associated with acidosis, as occurs during hypoxia, and traditionally viewed as deleterious1–3. However, recent studies contradict this view by demonstrating L-lactate's vital role as an int…
View article: Special Section Guest Editorial: Molecular Neurophotonics
Special Section Guest Editorial: Molecular Neurophotonics Open
The editorial introduces the Special Section on Molecular Neurophotonics.
View article: An Experimental Infection Model in Sheep and Goats to Evaluate Salmonella Colonization in Deep Tissue Lymph Nodes and after Carcass Vascular Rinsing with Bacteriophages in Goats
An Experimental Infection Model in Sheep and Goats to Evaluate Salmonella Colonization in Deep Tissue Lymph Nodes and after Carcass Vascular Rinsing with Bacteriophages in Goats Open
An animal infection model was evaluated on sheep and goats to confirm which species infected with Salmonella enterica serovar Enteritidis C StR (SE13) would provide a consistent and high frequency of Salmonella colonization in lymph nodes …
View article: Development of an miRFP680-Based Fluorescent Calcium Ion Biosensor Using End-Optimized Transposons
Development of an miRFP680-Based Fluorescent Calcium Ion Biosensor Using End-Optimized Transposons Open
The development of new or improved single fluorescent protein (FP)-based biosensors (SFPBs), particularly those with excitation and emission at near-infrared wavelengths, is important for the continued advancement of biological imaging app…
View article: Blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon absorption
Blue-shifted genetically encoded Ca2+ indicator with enhanced two-photon absorption Open
T-GECO1 is a high-performance blue-shifted GECI that, under two-photon excitation conditions, provides advantages relative to late generation GCaMP variants.
View article: An optogenetic method for the controlled release of single molecules
An optogenetic method for the controlled release of single molecules Open
We developed a system for optogenetic release of single molecules in cells. We confined soluble and transmembrane proteins to the Golgi apparatus via a photocleavable protein and released them by short pulses of light. Our method allows fo…
View article: High-Performance Genetically Encoded Green Fluorescent Biosensors for Intracellular <scp>l</scp>-Lactate
High-Performance Genetically Encoded Green Fluorescent Biosensors for Intracellular <span>l</span>-Lactate Open
l-Lactate is a monocarboxylate produced during the process of cellular glycolysis and has long generally been considered a waste product. However, studies in recent decades have provided new perspectives on the physiological roles of l-lac…
View article: Synthesis and application of a photocaged L-lactate
Synthesis and application of a photocaged L-lactate Open
l -Lactate, once considered a metabolic waste product of glycolysis, is now recognized as a vitally important metabolite and signaling molecule in multiple biological pathways. However, exploring l -lactate’s emerging intra- and extra-cell…