Eamonn Reading
YOU?
Author Swipe
View article: Molecular basis for multidrug efflux by an anaerobic-associated RND transporter
Molecular basis for multidrug efflux by an anaerobic-associated RND transporter Open
Bacteria can resist antibiotics and toxic substances within demanding ecological settings, such as low oxygen, extreme acid, and during nutrient starvation. MdtEF, a proton motive force-driven efflux pump from the resistance-nodulation-cel…
View article: Droplet microfluidic hydrogen/deuterium exchange for investigating protein dynamics with millisecond precision
Droplet microfluidic hydrogen/deuterium exchange for investigating protein dynamics with millisecond precision Open
Hydrogen/deuterium exchange (HDX) methods for studying protein dynamics would benefit from millisecond-scale incubations to probe intrinsically disordered proteins, highly dynamic regions and conformation changes. Here we investigate dropl…
View article: Molecular basis for multidrug efflux by an anaerobic RND transporter
Molecular basis for multidrug efflux by an anaerobic RND transporter Open
Bacteria can resist antibiotics and toxic substances within demanding ecological settings, such as low oxygen, extreme acid, and during nutrient starvation. MdtEF, a proton motive force-driven efflux pump from the resistance-nodulation-cel…
View article: Mg2+-dependent mechanism of environmental versatility in a multidrug efflux pump
Mg2+-dependent mechanism of environmental versatility in a multidrug efflux pump Open
Tripartite resistance nodulation and cell division multidrug efflux pumps span the periplasm and are major drivers of multidrug resistance among gram-negative bacteria. Cations, such as Mg2+, become concentrated within the periplasm and, i…
View article: Mg<sup>2+</sup>-dependent mechanism of environmental versatility in a multidrug efflux pump
Mg<sup>2+</sup>-dependent mechanism of environmental versatility in a multidrug efflux pump Open
Tripartite resistance nodulation and cell division multidrug efflux pumps span the periplasm and are a major driver of multidrug resistance among Gram-negative bacteria. The periplasm provides a distinct environment between the inner and o…
View article: Chromatographic Phospholipid Trapping for Automated H/D Exchange Mass Spectrometry of Membrane Protein–Lipid Assemblies
Chromatographic Phospholipid Trapping for Automated H/D Exchange Mass Spectrometry of Membrane Protein–Lipid Assemblies Open
Lipid interactions modulate the function, folding, structure, and organization of membrane proteins. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) has emerged as a useful tool to understand the structural dynamics of these protein…
View article: Structural mass spectrometry approaches to understand multidrug efflux systems
Structural mass spectrometry approaches to understand multidrug efflux systems Open
Multidrug efflux pumps are ubiquitous across both eukaryotes and prokaryotes, and have major implications in antimicrobial and multidrug resistance. They reside within cellular membranes and have proven difficult to study owing to their hy…
View article: Conformational restriction shapes inhibition of a multidrug efflux adaptor protein
Conformational restriction shapes inhibition of a multidrug efflux adaptor protein Open
Membrane efflux pumps play a major role in bacterial multidrug resistance. The tripartite multidrug efflux pump system from Escherichia coli , AcrAB-TolC, is a target for inhibition to lessen resistance development and restore antibiotic e…
View article: Chromatographic phospholipid trapping for automated H/D exchange mass spectrometry analysis of membrane protein-lipid assemblies
Chromatographic phospholipid trapping for automated H/D exchange mass spectrometry analysis of membrane protein-lipid assemblies Open
Lipid interactions modulate the function, folding, structure, and organization of membrane proteins. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) has emerged as a useful tool to understand the structural dynamics of these protein…
View article: Structural dynamics in the evolution of SARS-CoV-2 spike glycoprotein
Structural dynamics in the evolution of SARS-CoV-2 spike glycoprotein Open
SARS-CoV-2 spike glycoprotein mediates receptor binding and subsequent membrane fusion. It exists in a range of conformations, including a closed state unable to bind the ACE2 receptor, and an open state that does so but displays more expo…
View article: Perturbed structural dynamics underlie inhibition and altered efflux of the multidrug resistance pump AcrB
Perturbed structural dynamics underlie inhibition and altered efflux of the multidrug resistance pump AcrB Open
Resistance–nodulation–division efflux pumps play a key role in inherent and evolved multidrug resistance in bacteria. AcrB, a prototypical member of this protein family, extrudes a wide range of antimicrobial agents out of bacteria. Althou…
View article: Peptide-Based Approach to Inhibition of the Multidrug Resistance Efflux Pump AcrB
Peptide-Based Approach to Inhibition of the Multidrug Resistance Efflux Pump AcrB Open
Clinically relevant multidrug-resistant bacteria often arise due to overproduction of membrane-embedded efflux proteins that are capable of pumping antibiotics out of the bacterial cell before the drugs can exert their intended toxic effec…
View article: Capturing Membrane Protein Ribosome Nascent Chain Complexes in a Native-like Environment for Co-translational Studies
Capturing Membrane Protein Ribosome Nascent Chain Complexes in a Native-like Environment for Co-translational Studies Open
Co-translational folding studies of membrane proteins lag behind cytosolic protein investigations largely due to the technical difficulty in maintaining membrane lipid environments for correct protein folding. Stalled ribosome-bound nascen…
View article: Perturbed structural dynamics underlie inhibition and altered specificity of the multidrug efflux pump AcrB
Perturbed structural dynamics underlie inhibition and altered specificity of the multidrug efflux pump AcrB Open
Resistance-nodulation-division (RND) efflux pumps play a key role in inherent and evolved multidrug-resistance (MDR) in bacteria. AcrB is the prototypical member of the RND family and acts to recognise and export a wide range of chemically…
View article: Assessing Membrane Protein Structural Dynamics within Lipid Nanodiscs
Assessing Membrane Protein Structural Dynamics within Lipid Nanodiscs Open
Membrane protein structure, dynamics, and function have an intimate relationship with the complex and varied cellular lipid membranes they reside in. Lipid nanodisc technologies provide a membrane protein with a soluble lipid bilayer envir…
View article: Frontispiece: Structural Mass Spectrometry of Membrane Proteins within Their Native Lipid Environments
Frontispiece: Structural Mass Spectrometry of Membrane Proteins within Their Native Lipid Environments Open
The potential capability of structural mass spectrometry (MS) for deciphering membrane protein behavior within native environments has been highlighted. MS has become an important method for membrane protein structural biologists due to it…
View article: Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles
Photoinitiated Polymerization-Induced Self-Assembly in the Presence of Surfactants Enables Membrane Protein Incorporation into Vesicles Open
Photoinitiated polymerization-induced self-assembly (photo-PISA) is an efficient approach to predictably prepare polymeric nanostructures with a wide range of morphologies. Given that this process can be performed at high concentrations an…
View article: Lipid binding attenuates channel closure of the outer membrane protein OmpF
Lipid binding attenuates channel closure of the outer membrane protein OmpF Open
Significance Outer-membrane porins are often considered as passive conduits of small molecules across lipid bilayers. Using native mass spectrometry experiments we identify a pH-sensitive lipid-binding mechanism of outer membrane porin F, …
View article: Structural Mass Spectrometry of Membrane Proteins within Their Native Lipid Environments
Structural Mass Spectrometry of Membrane Proteins within Their Native Lipid Environments Open
Mass spectrometry has emerged as an important structural biology tool for understanding membrane protein structure, function, and dynamics. Generally, structural mass spectrometry of membrane proteins has been performed on purified or reco…
View article: Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs
Native mass spectrometry goes more native: investigation of membrane protein complexes directly from SMALPs Open
Other than more widely used methods, the use of styrene maleic acid copolymers allows the direct extraction of membrane proteins from the lipid bilayer into SMALPs keeping it in its native lipid surrounding.
View article: Interrogating Membrane Protein Conformational Dynamics within Native Lipid Compositions
Interrogating Membrane Protein Conformational Dynamics within Native Lipid Compositions Open
The interplay between membrane proteins and the lipids of the membrane is important for cellular function, however, tools enabling the interrogation of protein dynamics within native lipid environments are scarce and often invasive. We sho…
View article: Mechanistic insight into the assembly of the HerA–NurA helicase–nuclease DNA end resection complex
Mechanistic insight into the assembly of the HerA–NurA helicase–nuclease DNA end resection complex Open
The HerA-NurA helicase-nuclease complex cooperates with Mre11 and Rad50 to coordinate the repair of double-stranded DNA breaks. Little is known, however, about the assembly mechanism and activation of the HerA-NurA. By combining hybrid mas…