Ed Bergström
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Genes required for phosphosphingolipid formation in Caulobacter crescentus contribute to bacterial virulence Open
Sphingolipids are ubiquitous in membranes of eukaryotes and are associated with important cellular functions. Although sphingolipids occur scarcely in bacteria, for some of them they are essential and, in other bacteria, they contribute to…
View article: A medium‐throughput approach for improved taxonomic identification of lipids preserved in ancient pottery
A medium‐throughput approach for improved taxonomic identification of lipids preserved in ancient pottery Open
Organic residue analysis (ORA) is a valuable tool for the study of ancient diets, but conventional methods remain limited in terms of taxonomic identification or to resolve mixtures. Here, we propose a method to further explore a class of …
Genes required for the formation of virulence-provoking bacterial sphingolipids Open
Sphingolipids are ubiquitous in membranes of eukaryotes and are associated with important cellular functions. Although sphingolipids occur scarcely in bacteria, for some of them they are essential and, in other bacteria, they contribute to…
Formation of sulfoquinovosyl diacylglycerol by acylation of sulfoquinovosyl glycerol Open
Sulfoquinovosyl diacylglycerol (SQDG) is a membrane-forming lipid present in photosynthetic organisms as well as in distinct bacteria growing in phosphate-limited environments. Four genes for SQDG biosynthesis were previously identified in…
Pinus pinaster Early Hormonal Defence Responses to Pinewood Nematode (Bursaphelenchus xylophilus) Infection Open
The pinewood nematode (PWN) is the causal agent of pine wilt disease, a pathology that affects conifer forests, mainly Pinus spp. PWN infection can induce the expression of phytohormone-related genes; however, changes at the early phytohor…
Ionisation bias undermines the use of matrix‐assisted laser desorption/ionisation for estimating peptide deamidation: Synthetic peptide studies demonstrate electrospray ionisation gives more reliable response ratios Open
Rationale Although mass spectrometry (MS) is routinely used to determine deamination in peptide mixtures, the effects of the choice of ionisation source have not yet been investigated. In particular, matrix‐assisted laser desorption/ionisa…
Detection of opium alkaloids in a Cypriot base-ring juglet Open
The first rigorous chemical evidence supporting the controversial theory linking Bronze Age Cypriot base-ring juglets with the opium poppy.
One Filter, One Sample, and the <i>N</i>- and O-Glyco(proteo)me: Toward a System to Study Disorders of Protein Glycosylation Open
A method has been developed for release/isolation of O-glycans from glycoproteins in whole cell lysates for mass spectrometric analysis. Cells are lysed in SDS, which is then exchanged for urea and ammonium bicarbonate in a centrifugal fil…