Francisco E. Robles
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View article: Label-free in-line characterization of immune cell culture using quantitative phase imaging
Label-free in-line characterization of immune cell culture using quantitative phase imaging Open
Cell therapies, such as T cell immunotherapies, hold significant promise for treating complex diseases; however, their widespread adoption has been hindered by challenges related to monitoring cells during culture, which has affected their…
View article: Single Capture Quantitative Oblique Back-Illumination Microscopy
Single Capture Quantitative Oblique Back-Illumination Microscopy Open
Quantitative oblique back-illumination microscopy (qOBM) has emerged as a powerful technique for label-free, 3D quantitative phase imaging of arbitrarily thick biological specimens. However, in its initial embodiment, qOBM requires multipl…
View article: Non-destructive, high-resolution T cell characterization and subtyping via deep-ultraviolet microscopy
Non-destructive, high-resolution T cell characterization and subtyping via deep-ultraviolet microscopy Open
T cell characterization is critical for understanding immune function, monitoring disease progression, and optimizing cell-based therapies. Current technologies to characterize T cells, such as flow cytometry, require fluorescent labeling …
View article: Longitudinal and continuous label-free monitoring of glioblastoma patient-derived tumor spheroid treatment response using quantitative oblique back illumination microscopy
Longitudinal and continuous label-free monitoring of glioblastoma patient-derived tumor spheroid treatment response using quantitative oblique back illumination microscopy Open
Glioblastoma (GBM) is an aggressive brain tumor with limited treatment options and poor patient survival, underscoring the need for novel therapeutic strategies and improved preclinical models. Patient-derived tumor spheroids (PDTSs) offer…
View article: Label-Free In-Line Characterization of Immune Cell Culture using Quantitative Phase Imaging
Label-Free In-Line Characterization of Immune Cell Culture using Quantitative Phase Imaging Open
Cell therapies, including T cell immunotherapies, offer promising treatments for previously untreatable diseases, but their widespread use is hindered by challenges in monitoring therapeutic cells during culture -- impacting consistency, p…
View article: Acetic acid enabled nuclear contrast enhancement in epi-mode quantitative phase imaging
Acetic acid enabled nuclear contrast enhancement in epi-mode quantitative phase imaging Open
We show that the acetowhitening effect of acetic acid induces changes in refractive index composition that significantly enhance nuclear contrast in QPI. The application of qOBM with AA, along with the use of a Cycle-GAN algorithm to virtu…
View article: Experimental assessment of the optical transfer function for quantitative oblique back illumination microscopy (qOBM)
Experimental assessment of the optical transfer function for quantitative oblique back illumination microscopy (qOBM) Open
Quantitative oblique back illumination microscopy (qOBM) is a recently developed imaging technique that enables 3D quantitative phase imaging (QPI) and refractive index (RI) tomography of thick scattering samples. To quantify the phase and…
View article: Quantifying UV-induced photodamage for longitudinal live-cell imaging applications of deep-UV microscopy
Quantifying UV-induced photodamage for longitudinal live-cell imaging applications of deep-UV microscopy Open
Deep-UV microscopy enables high-resolution, label-free molecular imaging by leveraging biomolecular absorption properties in the UV spectrum. Recent advances in UV-imaging hardware have renewed interest in this technique for quantitative l…
View article: Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids
Non-invasive label-free imaging analysis pipeline for in situ characterization of 3D brain organoids Open
Brain organoids provide a unique opportunity to model organ development in a system similar to human organogenesis in vivo. Brain organoids thus hold great promise for drug screening and disease modeling. Conventional approaches to organoi…
View article: GAN-based quantitative oblique back-illumination microscopy enables computationally efficient epi-mode refractive index tomography
GAN-based quantitative oblique back-illumination microscopy enables computationally efficient epi-mode refractive index tomography Open
Quantitative oblique back-illumination microscopy (qOBM) is a novel imaging technology that enables epi-mode 3D quantitative phase imaging and refractive index (RI) tomography of thick scattering samples. The technology uses four oblique b…
View article: Non-Invasive Label-free Analysis Pipeline for In Situ Characterization of Differentiation in 3D Brain Organoid Models
Non-Invasive Label-free Analysis Pipeline for In Situ Characterization of Differentiation in 3D Brain Organoid Models Open
Brain organoids provide a unique opportunity to model organ development in a system similar to human organogenesis in vivo. Brain organoids thus hold great promise for drug screening and disease modeling. Conventional approaches to organoi…
View article: Label-Free Functional Analysis of Root-Associated Microbes with Dynamic Quantitative Oblique Back-illumination Microscopy
Label-Free Functional Analysis of Root-Associated Microbes with Dynamic Quantitative Oblique Back-illumination Microscopy Open
The increasing global demand for food, coupled with concerns about the environmental impact of synthetic fertilizers, underscores the urgency of developing sustainable agricultural practices. Nitrogen-fixing bacteria, known as diazotrophs,…
View article: Label- and slide-free tissue histology using 3D epi-mode quantitative phase imaging and virtual hematoxylin and eosin staining
Label- and slide-free tissue histology using 3D epi-mode quantitative phase imaging and virtual hematoxylin and eosin staining Open
Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis and comprehensive clinical assessment of tissue. However, the typical formalin-fixation, paraffin-embe…
View article: Analysis of structural effects of sickle cell disease on brain vasculature of mice using three-dimensional quantitative phase imaging
Analysis of structural effects of sickle cell disease on brain vasculature of mice using three-dimensional quantitative phase imaging Open
qOBM provides insight into the biophysical and structural composition of brain blood vessels in mice with SCD. Data suggest that the RI may be an indirect indicator of vessel rigidity, vessel strength, and/or tensions, which change with SC…
View article: Label- and slide-free tissue histology using 3D epi-mode quantitative phase imaging and virtual H&E staining.
Label- and slide-free tissue histology using 3D epi-mode quantitative phase imaging and virtual H&E staining. Open
Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis and comprehensive clinical assessment of tissue. However, the process is laborious and time-consuming,…
View article: Label- and slide-free tissue histology using 3D epi-mode quantitative phase imaging and virtual H&E staining
Label- and slide-free tissue histology using 3D epi-mode quantitative phase imaging and virtual H&E staining Open
Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis and comprehensive clinical assessment of tissue. However, the process is laborious and time-consuming,…
View article: Partially coherent broadband 3D optical transfer functions with arbitrary temporal and angular power spectra
Partially coherent broadband 3D optical transfer functions with arbitrary temporal and angular power spectra Open
Optical diffraction tomography is a powerful technique to produce 3D volumetric images of biological samples using contrast produced by variations in the index of refraction in an unlabeled specimen. While this is typically performed with …
View article: Compact and low-cost deep-ultraviolet microscope system for label-free molecular imaging and point-of-care hematological analysis
Compact and low-cost deep-ultraviolet microscope system for label-free molecular imaging and point-of-care hematological analysis Open
Deep-ultraviolet (UV) microscopy enables label-free, high-resolution, quantitative molecular imaging and enables unique applications in biomedicine, including the potential for fast hematological analysis at the point-of-care. UV microscop…
View article: Functional imaging with dynamic quantitative oblique back-illumination microscopy
Functional imaging with dynamic quantitative oblique back-illumination microscopy Open
In this work we present a label-free, fast, and scalable functional imaging approach to study and intuitively display cellular and subcellular dynamics. We further show the potential utility of this novel technique to help monitor adherent…
View article: Optimization of a flexible fiber-optic probe for epi-mode quantitative phase imaging
Optimization of a flexible fiber-optic probe for epi-mode quantitative phase imaging Open
Quantitative oblique back-illumination microscopy (qOBM) is an emerging label-free optical imaging technology that enables 3D, tomographic quantitative phase imaging (QPI) with epi-illumination in thick scattering samples. In this work, we…
View article: Deep UV Microscopy Identifies Prostatic Basal Cells: An Important Biomarker for Prostate Cancer Diagnostics
Deep UV Microscopy Identifies Prostatic Basal Cells: An Important Biomarker for Prostate Cancer Diagnostics Open
Objective and Impact Statement . Identifying benign mimics of prostatic adenocarcinoma remains a significant diagnostic challenge. In this work, we developed an approach based on label-free, high-resolution molecular imaging with multispec…
View article: Virtual Staining, Segmentation, and Classification of Blood Smears for Label-Free Hematology Analysis
Virtual Staining, Segmentation, and Classification of Blood Smears for Label-Free Hematology Analysis Open
Objective and Impact Statement . We present a fully automated hematological analysis framework based on single-channel (single-wavelength), label-free deep-ultraviolet (UV) microscopy that serves as a fast, cost-effective alternative to co…
View article: Prostate Cancer Histopathology Using Label-free Multispectral Deep-UV Microscopy Quantifies Phenotypes of Tumor Aggressiveness and Enables Multiple Diagnostic Virtual Stains
Prostate Cancer Histopathology Using Label-free Multispectral Deep-UV Microscopy Quantifies Phenotypes of Tumor Aggressiveness and Enables Multiple Diagnostic Virtual Stains Open
Identifying prostate cancer patients that are harboring aggressive forms of prostate cancer remains a significant clinical challenge. Here we develop an approach based on multispectral deep-ultraviolet (UV) microscopy that provides novel q…
View article: Declaraciones públicas de cámaras empresariales de América Central en el contexto COVID-19 (Marzo-Septiembre, 2020)
Declaraciones públicas de cámaras empresariales de América Central en el contexto COVID-19 (Marzo-Septiembre, 2020) Open
The present database is developed within the framework of the project "¿Puertas o candados? Pandemia, políticas públicas e influencia empresarial en América Central" of the Institute of Social Research of the University of Costa Rica (IIS-…
View article: Label-free automated neutropenia detection and grading using deep-ultraviolet microscopy
Label-free automated neutropenia detection and grading using deep-ultraviolet microscopy Open
Neutropenia is a condition identified by an abnormally low number of neutrophils in the bloodstream and signifies an increased risk of severe infection. Cancer patients are particularly susceptible to this condition, which can be disruptiv…