Edward E. K. Baidoo
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View article: Enhancement of sorghum biomass hydrolysis by addition of amylases to an ionic liquid-based deconstruction process
Enhancement of sorghum biomass hydrolysis by addition of amylases to an ionic liquid-based deconstruction process Open
We found that amylases are compatible with cholinium-based ionic liquids and act synergistically with other saccharolytic enzymes to deconstruct sorghum biomass. By using mixtures of sorghum grain (high starch content) and stover (low star…
View article: Engineered Accumulation of Protocatechuate in Corn Biomass to Enhance Biomanufacturing
Engineered Accumulation of Protocatechuate in Corn Biomass to Enhance Biomanufacturing Open
The in-planta accumulation of coproducts in crops can enhance the value of lignocellulosic biomass and facilitate a sustainable bioeconomy. Corn stover represents a major renewable source of lignocellulose for the production of advanced bi…
View article: Predictive CRISPR-mediated gene downregulation for enhanced production of sustainable aviation fuel precursor in Pseudomonas putida
Predictive CRISPR-mediated gene downregulation for enhanced production of sustainable aviation fuel precursor in Pseudomonas putida Open
CRISPR interference (CRISPRi) has emerged as a valuable tool for redirecting metabolic flux to enhance bioproduction. However, its application is often constrained by two challenges: (i) rationally identifying effective gene targets for do…
View article: Engineering Polyketide Stereocenters with Ketoreductase Domain Exchanges
Engineering Polyketide Stereocenters with Ketoreductase Domain Exchanges Open
Polyketide synthases (PKSs) are versatile biosynthetic megasynthases capable of producing a diverse range of natural products with many applications, including in pharmaceuticals. The stereochemical precision of PKSs makes them a powerful …
View article: The mevalonate pathway of isoprenoid biosynthesis supports metabolic flexibility in <i>Mycobacterium marinum</i>
The mevalonate pathway of isoprenoid biosynthesis supports metabolic flexibility in <i>Mycobacterium marinum</i> Open
Isoprenoids are a diverse class of natural products that are essential in all domains of life. Most bacteria synthesize isoprenoids through either the methylerythritol phosphate (MEP) pathway or the mevalonate (MEV) pathway, while a small …
View article: Biosensor-driven strain engineering reveals key cellular processes for maximizing isoprenol production in <i>Pseudomonas putida</i>
Biosensor-driven strain engineering reveals key cellular processes for maximizing isoprenol production in <i>Pseudomonas putida</i> Open
Synthetic biology generates vast combinatorial designs, yet high-throughput analytical methods to screen them are poorly matched to interrogate this search space. We address this challenge by developing a biosensor-driven, growth-coupled s…
View article: The anaerobic fungus Neocallimastix californiae shifts metabolism and produces melanin in response to lignin-derived aromatic compounds
The anaerobic fungus Neocallimastix californiae shifts metabolism and produces melanin in response to lignin-derived aromatic compounds Open
Background Biological deconstruction of lignocellulose for sustainable chemical production offers an opportunity to harness evolutionarily specialized enzymes and organisms for industrial bioprocessing. While hydrolysis of cellulose and he…
View article: The mevalonate pathway of isoprenoid biosynthesis supports metabolic flexibility in <i>Mycobacterium marinum</i>
The mevalonate pathway of isoprenoid biosynthesis supports metabolic flexibility in <i>Mycobacterium marinum</i> Open
Isoprenoids are a diverse class of natural products that are essential in all domains of life. Most bacteria synthesize isoprenoids through either the methylerythritol phosphate (MEP) pathway or the mevalonate (MEV) pathway, while a small …
View article: Causes and consequences of experimental variation in <i>Nicotiana benthamiana</i> transient expression
Causes and consequences of experimental variation in <i>Nicotiana benthamiana</i> transient expression Open
Infiltration of Agrobacterium tumefaciens into Nicotiana benthamiana has become a foundational technique in plant biology, enabling efficient delivery of transgenes in planta with technical ease, robust signal, and relatively high throughp…
View article: Predictive genome-wide CRISPR-mediated gene downregulation for enhanced bioproduction
Predictive genome-wide CRISPR-mediated gene downregulation for enhanced bioproduction Open
CRISPR interference (CRISPRi) has emerged as a valuable tool for redirecting metabolic flux to enhance bioproduction. However, conventional approaches for identifying target genes for CRISPRi-mediated downregulation have largely relied on …
View article: Retrobiosynthesis of unnatural lactams via reprogrammed polyketide synthase
Retrobiosynthesis of unnatural lactams via reprogrammed polyketide synthase Open
Engineered polyketide synthases (PKSs) have great potential as biocatalysts. These unnatural enzymes are capable of synthesizing molecules that are either not amenable to biosynthesis or are extremely challenging to access chemically. PKSs…
View article: In planta production of the nylon precursor beta-ketoadipate
In planta production of the nylon precursor beta-ketoadipate Open
Beta-ketoadipate (βKA) is an intermediate of the βKA pathway involved in the degradation of aromatic compounds in several bacteria and fungi. Beta-ketoadipate also represents a promising chemical for the manufacturing of performance-advant…
View article: Biosensor-Driven Strain Engineering Reveals Key Cellular Processes for Maximizing Isoprenol Production in <i>Pseudomonas putida</i>
Biosensor-Driven Strain Engineering Reveals Key Cellular Processes for Maximizing Isoprenol Production in <i>Pseudomonas putida</i> Open
Synthetic biology tools have accelerated the generation of simple mutants, but combinatorial testing remains challenging. High-throughput methods struggle translating from proof-of-principle molecules to advanced bioproducts. We address th…
View article: A polyketide-based biosynthetic platform for diols, amino alcohols and hydroxy acids
A polyketide-based biosynthetic platform for diols, amino alcohols and hydroxy acids Open
Medium- and branched-chain diols and amino alcohols are important industrial solvents, polymer building blocks, cosmetics and pharmaceutical ingredients, yet biosynthetically challenging to produce. Here we present an approach that uses a …
View article: Reversed phase LC-MS analysis of organic acids involved in the tricarboxylic acid cycle v2
Reversed phase LC-MS analysis of organic acids involved in the tricarboxylic acid cycle v2 Open
Organic acids of primary metabolism are essential for energy metabolism. They can range from formate (C1), glyoxylate (C2), to citrate (C6), or greater. While reversed phase (RP) C18 chromatography may not be the ideal separation technique…
View article: The importance of ester cleavage in the butylamine pretreatment of hybrid poplar
The importance of ester cleavage in the butylamine pretreatment of hybrid poplar Open
Butylamine is an effective in-and-out pretreatment solvent for hybrid poplar. It penetrates the cell walls and breaks ester cross-linkages, facilitating lignin release and improving enzymatic digestion.
View article: A polyketide-based biosynthetic platform for diols, amino alcohols and hydroxyacids
A polyketide-based biosynthetic platform for diols, amino alcohols and hydroxyacids Open
Medium- and branched-chain diols and amino alcohols are important industrial solvents, polymer building blocks, cosmetics and pharmaceutical ingredients, yet biosynthetically challenging to produce. Here, we present a novel approach utilis…
View article: Engineered reduction of S-adenosylmethionine alters lignin in sorghum
Engineered reduction of S-adenosylmethionine alters lignin in sorghum Open
Background Lignin is an aromatic polymer deposited in secondary cell walls of higher plants to provide strength, rigidity, and hydrophobicity to vascular tissues. Due to its interconnections with cell wall polysaccharides, lignin plays imp…
View article: Multi-omics analysis of a traditional fermented food reveals a byproduct-associated subpopulation of<i>Neurospora intermedia</i>for waste-to-food upcycling
Multi-omics analysis of a traditional fermented food reveals a byproduct-associated subpopulation of<i>Neurospora intermedia</i>for waste-to-food upcycling Open
Fungal solid-state fermentation (SSF) of byproducts has promise for increasing food sustainability and security, but fungal waste-to-food upcycling remains poorly understood at the molecular level. Here we use a multi-omics approach to cha…
View article: Systematic engineering for production of anti-aging sunscreen compound in Pseudomonas putida
Systematic engineering for production of anti-aging sunscreen compound in Pseudomonas putida Open
Sunscreen has been used for thousands of years to protect skin from ultraviolet radiation. However, the use of modern commercial sunscreen containing oxybenzone, ZnO, and TiO2 has raised concerns due to their negative effects on human heal…
View article: Bottlenecks in the Implementation of Genome Scale Metabolic Model Based Designs for Bioproduction from Aromatic Carbon Sources
Bottlenecks in the Implementation of Genome Scale Metabolic Model Based Designs for Bioproduction from Aromatic Carbon Sources Open
Genome scale metabolic models (GSMM) are commonly used to identify gene deletion sets that result in growth coupling, pairing product formation with substrate utilization. While such approaches can improve strain performance beyond levels …
View article: Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit
Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit Open
Filamentous fungi are critical in the transition to a more sustainable food system. While genetic modification of these organisms has promise for enhancing the nutritional value, sensory appeal, and scalability of fungal foods, genetic too…