Edward G. Ruby
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View article: Nanoscale imaging of native symbiotic animal tissue using a multimodal large volume imaging pipeline for cryo-electron tomography
Nanoscale imaging of native symbiotic animal tissue using a multimodal large volume imaging pipeline for cryo-electron tomography Open
The field of cryo-EM offers the possibility to gain high-resolution structural information of biomolecules in their native state. Advances in sample thinning of cryo-EM samples allows the study of proteins inside intact cells using tomogra…
View article: Strain matters: host responses reflect symbiont origin in the squid-vibrio symbiosis
Strain matters: host responses reflect symbiont origin in the squid-vibrio symbiosis Open
Understanding the cause and consequences of bacterial strain variation remains a challenge in the study of symbioses. While the diverse reactions of the host immune system to strain variants have been well studied in pathogenesis, much les…
View article: Mutant <i>gltS</i> alleles enable a <i>Vibrio fischeri</i> D-glutamate auxotroph to grow with lower requirements for exogenous D-glutamate
Mutant <i>gltS</i> alleles enable a <i>Vibrio fischeri</i> D-glutamate auxotroph to grow with lower requirements for exogenous D-glutamate Open
D-glu is a key component of peptidoglycan (PG) and is essential for growth in most bacteria. To assess constraints on PG evolution and bacterial requirements for D-glu, we sought to artificially evolve PG biosynthesis, leading to either re…
View article: Climate‐Driven Warming Disrupts the Symbiosis of Bobtail Squid <i>Euprymna scolopes</i> and the Luminous Bacterium <i>Vibrio fischeri</i>
Climate‐Driven Warming Disrupts the Symbiosis of Bobtail Squid <i>Euprymna scolopes</i> and the Luminous Bacterium <i>Vibrio fischeri</i> Open
Under the current climate crisis, marine heatwaves (MHW) are expected to intensify and become more frequent in the future, leading to adverse effects on marine life. Here, we aimed to investigate the impact of environmental warming on the …
View article: Application of <i>hsp60</i> amplicon sequencing to characterize microbial communities associated with juvenile and adult <i>Euprymna scolopes</i> squid
Application of <i>hsp60</i> amplicon sequencing to characterize microbial communities associated with juvenile and adult <i>Euprymna scolopes</i> squid Open
The symbiotic relationship between Vibrio (Aliivibrio) fischeri and the Hawaiian bobtail squid, Euprymna scolopes, serves as a key model for understanding host–microbe interactions. Traditional culture-based methods have primarily isolated…
View article: An acidic microenvironment produced by the V-type ATPase of Euprymna scolopes promotes specificity during Vibrio fischeri recruitment
An acidic microenvironment produced by the V-type ATPase of Euprymna scolopes promotes specificity during Vibrio fischeri recruitment Open
Animals often acquire their microbial symbionts from the environment, but the mechanisms underlying how specificity of the association is achieved are poorly understood. We demonstrate that the conserved proton pump, V-type ATPase (VHA), p…
View article: Developmental and transcriptomic responses of Hawaiian bobtail squid early stages to ocean warming and acidification
Developmental and transcriptomic responses of Hawaiian bobtail squid early stages to ocean warming and acidification Open
1 Abstract Cephalopods play a central ecological role across all oceans and realms. However, under the current climate crisis, their physiology and behaviour are impacted, and we are beginning to comprehend the effects of environmental str…
View article: Application of<i>hsp60</i>amplicon sequencing to characterize microbial communities associated with juvenile and adult<i>Euprymna scolopes</i>squid
Application of<i>hsp60</i>amplicon sequencing to characterize microbial communities associated with juvenile and adult<i>Euprymna scolopes</i>squid Open
The symbiotic relationship between Vibrio (Aliivibrio) fischeri and the Hawaiian bobtail squid, Euprymna scolopes , serves as a key model for understanding host-microbe interactions. Traditional culture-based methods have primarily isolate…
View article: Transient infection of <i>Euprymna scolopes</i> with an engineered D-alanine auxotroph of <i>Vibrio fischeri</i>
Transient infection of <i>Euprymna scolopes</i> with an engineered D-alanine auxotroph of <i>Vibrio fischeri</i> Open
The symbiosis between Vibrio fischeri and the Hawaiian bobtail squid, Euprymna scolopes , is a tractable and well-studied model of bacteria-animal mutualism. Here, we developed a method to transiently colonize E. scolopes using D-alanine (…
View article: Bacterial growth dynamics in a rhythmic symbiosis
Bacterial growth dynamics in a rhythmic symbiosis Open
The symbiotic relationship between the bioluminescent bacterium Vibrio fischeri and the bobtail squid Euprymna scolopes serves as a valuable system to investigate bacterial growth and peptidoglycan (PG) synthesis within animal tissues. To …
View article: A new lexicon in the age of microbiome research
A new lexicon in the age of microbiome research Open
At a rapid pace, biologists are learning the many ways in which resident microbes influence, and sometimes even control, their hosts to shape both health and disease. Understanding the biochemistry behind these interactions promises to rev…
View article: Maturation state of colonization sites promotes symbiotic resiliency in the Euprymna scolopes-Vibrio fischeri partnership
Maturation state of colonization sites promotes symbiotic resiliency in the Euprymna scolopes-Vibrio fischeri partnership Open
Background Many animals and plants acquire their coevolved symbiotic partners shortly post-embryonic development. Thus, during embryogenesis, cellular features must be developed that will promote both symbiont colonization of the appropria…
View article: RF23 | PSAT306 Thyrotoxic Periodic Paralysis: An Uncommon Complication Presenting From an Unsuspected Demographic
RF23 | PSAT306 Thyrotoxic Periodic Paralysis: An Uncommon Complication Presenting From an Unsuspected Demographic Open
Background Thyrotoxic Periodic Paralysis (TPP) is a rare complication of thyrotoxicosis mostly reported in Eastern Asian countries with relatively few known cases among non-Asian diaspora in the West. Case A 28-year-old African American ma…
View article: ODP106 Mystery of the Calcium: A Case of Exclusive Cutaneous Sarcoidosis presenting with Symptomatic Hypercalcemia
ODP106 Mystery of the Calcium: A Case of Exclusive Cutaneous Sarcoidosis presenting with Symptomatic Hypercalcemia Open
The skin is the second most involved organ after the lung in sarcoidosis. It could be an early manifestation that may progress to systemic disease later. Exclusive cutaneous disease is more frequently seen in females. Specific lesions incl…
View article: Ciliated epithelia are key elements in the recruitment of bacterial partners in the squid-vibrio symbiosis
Ciliated epithelia are key elements in the recruitment of bacterial partners in the squid-vibrio symbiosis Open
The Hawaiian bobtail squid, Euprymna scolopes , harvests its luminous symbiont, Vibrio fischeri , from the surrounding seawater within hours of hatching. During embryogenesis, the host animal develops a nascent light organ with ciliated fi…
View article: Reply to Oren et al., “New Phylum Names Harmonize Prokaryotic Nomenclature”
Reply to Oren et al., “New Phylum Names Harmonize Prokaryotic Nomenclature” Open
We thank Oren et al., 2022 (1) for providing the International Committee on Systematics of Prokaryotes (ICSP) Executive Board's response to the recent opinion piece “Harmonizing Prokaryotic Nomenclature: Fixing the Fuss over Phylum Name Fl…
View article: Transitioning to confined spaces impacts bacterial swimming and escape response
Transitioning to confined spaces impacts bacterial swimming and escape response Open
View article: Evidence of Genomic Diversification in a Natural Symbiotic Population Within Its Host
Evidence of Genomic Diversification in a Natural Symbiotic Population Within Its Host Open
Planktonic cells of the luminous marine bacterium Vibrio fischeri establish themselves in the light-emitting organ of each generation of newly hatched Euprymna scolopes bobtail squid. A symbiont population is maintained within the 6 separa…
View article: Independent host- and bacterium-based determinants protect a model symbiosis from phage predation
Independent host- and bacterium-based determinants protect a model symbiosis from phage predation Open
View article: Bacterial Quorum-Sensing Regulation Induces Morphological Change in a Key Host Tissue during the Euprymna scolopes-Vibrio fischeri Symbiosis
Bacterial Quorum-Sensing Regulation Induces Morphological Change in a Key Host Tissue during the Euprymna scolopes-Vibrio fischeri Symbiosis Open
Interbacterial signaling within a host-associated population can have profound effects on the behavior of the bacteria, for instance, in their production of virulence/colonization factors; in addition, such signaling can dictate the nature…
View article: Getting the Message Out: the Many Modes of Host-Symbiont Communication during Early-Stage Establishment of the Squid-Vibrio Partnership
Getting the Message Out: the Many Modes of Host-Symbiont Communication during Early-Stage Establishment of the Squid-Vibrio Partnership Open
Symbiosis, by its basic nature, depends on partner interactions that are mediated by cues and signals. This kind of critical reciprocal communication shapes the trajectory of host-microbe associations from their onset through their maturat…
View article: Transitioning to confined spaces impacts bacterial swimming and escape response
Transitioning to confined spaces impacts bacterial swimming and escape response Open
Symbiotic bacteria often navigate complex environments before colonizing privileged sites in their host organism. Chemical gradients are known to facilitate directional taxis of these bacteria, guiding them towards their eventual destinati…
View article: Independent host- and bacterium-based determinants protect a model symbiosis from phage predation
Independent host- and bacterium-based determinants protect a model symbiosis from phage predation Open
Summary/Abstract Bacteriophages (phages) are diverse and abundant constituents of microbial communities worldwide, and are capable of modulating bacterial populations in diverse ways. Here we describe a novel phage, ϕHNL01, which infects t…
View article: A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host
A lasting symbiosis: how Vibrio fischeri finds a squid partner and persists within its natural host Open
View article: MicroRNA-Mediated Regulation of Initial Host Responses in a Symbiotic Organ
MicroRNA-Mediated Regulation of Initial Host Responses in a Symbiotic Organ Open
Animals often acquire their microbiome from the environment at each generation, making the initial interaction of the partners a critical event in the establishment and development of a stable, healthy symbiosis. However, the molecular nat…
View article: Modeled microgravity alters lipopolysaccharide and outer membrane vesicle production of the beneficial symbiont Vibrio fischeri
Modeled microgravity alters lipopolysaccharide and outer membrane vesicle production of the beneficial symbiont Vibrio fischeri Open
View article: Animals in a bacterial world, a new imperative for the life sciences
Animals in a bacterial world, a new imperative for the life sciences Open
In the last two decades, the widespread application of genetic and genomic approaches has revealed a bacterial world astonishing in its ubiquity and diversity. This review examines how a growing knowledge of the vast range of animal–bacter…
View article: The noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses
The noncoding small RNA SsrA is released by Vibrio fischeri and modulates critical host responses Open
The regulatory noncoding small RNAs (sRNAs) of bacteria are key elements influencing gene expression; however, there has been little evidence that beneficial bacteria use these molecules to communicate with their animal hosts. We report he…
View article: The cytokine MIF controls daily rhythms of symbiont nutrition in an animal–bacterial association
The cytokine MIF controls daily rhythms of symbiont nutrition in an animal–bacterial association Open
Significance Daily biological rhythms are fundamental, evolutionarily conserved features of many symbioses. Here we use the binary squid–vibrio association, the simplicity of which has offered the resolution to explore strategies of symbio…
View article: HbtR, a Heterofunctional Homolog of the Virulence Regulator TcpP, Facilitates the Transition between Symbiotic and Planktonic Lifestyles in Vibrio fischeri
HbtR, a Heterofunctional Homolog of the Virulence Regulator TcpP, Facilitates the Transition between Symbiotic and Planktonic Lifestyles in Vibrio fischeri Open
TcpP homologs are widespread throughout the Vibrio genus; however, the only protein in this family described thus far is a V. cholerae virulence regulator. Here, we show that HbtR, the TcpP homolog in V. fischeri , has both a biological ro…