Explanipedia

Publisher Correction: Multimodal cell maps as a foundation for structural and functional genomics Open

Leah V. Schaffer, Mengzhou Hu, Gege Qian, Kyung‐Mee Moon, Abantika Pal , et al. · 2025

Covariation MS uncovers a protein that controls cysteine catabolism Open

Haopeng Xiao, Martha Ordonez, Emma C. Fink, Taylor A. Covington, Hilina Woldemichael , et al. · 2025

Multimodal cell maps as a foundation for structural and functional genomics Open

Leah V. Schaffer, Mengzhou Hu, Gege Qian, Kyung‐Mee Moon, Abantika Pal , et al. · 2025

Human cells consist of a complex hierarchy of components, many of which remain unexplored 1,2 . Here we construct a global map of human subcellular architecture through joint measurement of biophysical interactions and immunofluorescence i…

Altered Fibroblast Glutamine Metabolism Is Linked to the Severity of Cardiac Dysfunction in <span>DCMA</span> , a Mitochondrial Cardiomyopathy Open

Melissa A. King, Katherine Heger, Marija Drikic, Ayush Mandwal, Aneal Khan , et al. · 2025

The dilated cardiomyopathy with ataxia (DCMA) syndrome is a rare mitochondrial disorder caused by mutations in the poorly understood DNAJC19 gene. Cardiac involvement in DCMA ranges from mild conduction abnormalities to early severe myocar…

Profiling Proteins and Phosphorylation Sites During T Cell Activation Using an Integrated Thermal Shift Assay Open

Brandon M. Gassaway, Edward L. Huttlin, Emily M. Huntsman, Tomer M. Yaron-Barir, Jared L. Johnson , et al. · 2024

Quantitative proteomics defines mechanisms of antiviral defence and cell death during modified vaccinia Ankara infection Open

Jonas D. Albarnaz, Joanne Kite, Marisa Oliveira, Hanqi Li, Ying Di , et al. · 2023

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation Open

Stephanie Pei Tung Yiu, Cassie Zerbe, David Vanderwall, Edward L. Huttlin, Michael P. Weekes , et al. · 2023

Proteomic analysis of circulating immune cells identifies cellular phenotypes associated with COVID-19 severity Open

Martin Potts, Alice Fletcher-Etherington, Katie Nightingale, Federica Mescia, Laura Bergamaschi , et al. · 2023

Certain serum proteins, including C-reactive protein (CRP) and D-dimer, have prognostic value in patients with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Nonetheless, these factors are non-specific, providing limited mec…

Human Polo-like Kinase Inhibitors as Antiplasmodials Open

Monica J. Bohmer, Jinhua Wang, Eva S. Istvan, Madeline R. Luth, Jennifer E. Collins , et al. · 2023

Protein kinases have proven to be a very productive class of therapeutic targets, and over 90 inhibitors are currently in clinical use primarily for the treatment of cancer. Repurposing these inhibitors as antimalarials could provide an ac…

BioPlexR and BioPlexPy: integrated data products for the analysis of human protein interactions Open

Ludwig Geistlinger, Roger Vargas, Tyrone Lee, Joshua Pan, Edward L. Huttlin , et al. · 2023

Summary The BioPlex project has created two proteome scale, cell-line-specific protein–protein interaction (PPI) networks: the first in 293T cells, including 120k interactions among 15k proteins; and the second in HCT116 cells, including 7…

Sample multiplexing-based targeted pathway proteomics with real-time analytics reveals the impact of genetic variation on protein expression Open

Qing Yu, Xinyue Liu, Mark P. Keller, Jose Navarrete‐Perea, Tian Zhang , et al. · 2023

A proteome-wide atlas of drug mechanism of action Open

Dylan C. Mitchell, Miljan Kuljanin, Jiaming Li, Jonathan G. Van Vranken, Nathan J. Bulloch , et al. · 2023

Architecture of the outbred brown fat proteome defines regulators of metabolic physiology Open

Haopeng Xiao, Luiz H. M. Bozi, Yizhi Sun, Christopher L. Riley, Vivek M. Philip , et al. · 2022

A multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics Open

Brandon M. Gassaway, Jiaming Li, Ramin Rad, Julian Mintseris, Kyle Mohler , et al. · 2022

Quantitative temporal analysis of modified vaccinia Ankara, the monkeypox and smallpox vaccine Open

Jonas D. Albarnaz, Marisa Oliveira, Joanne Kite, João A. Paulo, Robin Antrobus , et al. · 2022

Modified vaccinia Ankara (MVA) immunisation is being deployed to curb the current outbreak of monkeypox in multiple countries1. Originally authorized for vaccination against smallpox, MVA is a vaccinia virus (VACV) strain that does not rep…

Author Correction: Defining the consequences of genetic variation on a proteome-wide scale Open

Joel M. Chick, Steven C. Munger, Petr Šimeček, Edward L. Huttlin, Kwangbom Choi , et al. · 2022

A multi-scale map of cell structure fusing protein images and interactions Open

Yue Qin, Edward L. Huttlin, Casper F. Winsnes, Maya L. Gosztyla, Ludivine Wacheul , et al. · 2021

Proteome-wide mapping of short-lived proteins in human cells Open

Jiaming Li, Zhenying Cai, Laura Pontano Vaites, Ning Shen, Dylan C. Mitchell , et al. · 2021

Dual proteome-scale networks reveal cell-specific remodeling of the human interactome Open

Edward L. Huttlin, Raphael J. Bruckner, Jose Navarrete‐Perea, Joe R. Cannon, Kurt Baltier , et al. · 2021

Altered glutamine metabolism of cultured fibroblasts predicts severity of cardiac dysfunction in the dilated cardiomyopathy with ataxia syndrome (DCMA), a mitochondrial cardiomyopathy Open

Melissa A. King, Katherine Heger, Aneal Khan, David S. Sinasac, Edward L. Huttlin , et al. · 2020

Dilated cardiomyopathy with ataxia (DCMA) syndrome is a rare mitochondrial disorder caused by mutations in the poorly understood DNAJC19 gene. The clinical presentation of DCMA is very diverse with symptoms ranging from mild cardiac dysfun…

Mapping cell structure across scales by fusing protein images and interactions Open

Yue Qin, Casper F. Winsnes, Edward L. Huttlin, Fan Zheng, Wei Ouyang , et al. · 2020

The eukaryotic cell is a multi-scale structure with modular organization across at least four orders of magnitude 1,2 . Two central approaches for mapping this structure – protein fluorescent imaging and protein biophysical association – e…

Selection of Heating Temperatures Improves the Sensitivity of the Proteome Integral Solubility Alteration Assay Open

Jiaming Li, Jonathan G. Van Vranken, João A. Paulo, Edward L. Huttlin, Steven P. Gygi · 2020

The thermal shift assay is a robust method of discovering protein-ligand interactions by measuring the alterations in protein thermal stability under various conditions. Several thermal shift assays have been developed and their throughput…

TMTpro reagents: a set of isobaric labeling mass tags enables simultaneous proteome-wide measurements across 16 samples Open

Jiaming Li, Jonathan G. Van Vranken, Laura Pontano Vaites, Devin K. Schweppe, Edward L. Huttlin , et al. · 2020

Full-Featured, Real-Time Database Searching Platform Enables Fast and Accurate Multiplexed Quantitative Proteomics Open

Devin K. Schweppe, Jimmy K. Eng, Qing Yu, Derek J. Bailey, Ramin Rad , et al. · 2020

Multiplexed quantitative analyses of complex proteomes enable deep biological insight. While a multitude of workflows have been developed for multiplexed analyses, the most quantitatively accurate method (SPS-MS3) suffers from long acquisi…

A Quantitative Tissue-Specific Landscape of Protein Redox Regulation during Aging Open

Haopeng Xiao, Mark P. Jedrychowski, Devin K. Schweppe, Edward L. Huttlin, Qing Yu , et al. · 2020

Dual Proteome-scale Networks Reveal Cell-specific Remodeling of the Human Interactome Open

Edward L. Huttlin, Raphael J. Bruckner, Jose Navarrete‐Perea, Joe R. Cannon, Kurt Baltier , et al. · 2020

SUMMARY Thousands of interactions assemble proteins into modules that impart spatial and functional organization to the cellular proteome. Through affinity-purification mass spectrometry, we have created two proteome-scale, cell-line-speci…

Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions Open

Luís Nobre, Katie Nightingale, Benjamin J. Ravenhill, Robin Antrobus, Lior Soday , et al. · 2019

Human cytomegalovirus (HCMV) extensively modulates host cells, downregulating >900 human proteins during viral replication and degrading ≥133 proteins shortly after infection. The mechanism of degradation of most host proteins remains unre…

Author response: Human cytomegalovirus interactome analysis identifies degradation hubs, domain associations and viral protein functions Open

Luís Nobre, Katie Nightingale, Benjamin J. Ravenhill, Robin Antrobus, Lior Soday , et al. · 2019

Article Figures and data Abstract Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Human cytomegalovirus (HCMV) extensively m…

Investigation of Proteomic and Phosphoproteomic Responses to Signaling Network Perturbations Reveals Functional Pathway Organizations in Yeast Open

Jiaming Li, João A. Paulo, David P. Nusinow, Edward L. Huttlin, Steven P. Gygi · 2019

Full-featured, real-time database searching platform enables fast and accurate multiplexed quantitative proteomics Open

Devin K. Schweppe, Jimmy K. Eng, Derek J. Bailey, Ramin Rad, Qing Yu , et al. · 2019

Multiplexed quantitative analyses of complex proteomes enable deep biological insight. While a multitude of workflows have been developed for multiplexed analyses, the most quantitatively accurate method (SPS-MS3) suffers from long acquisi…

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