Edward N. Baker
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View article: Protease mimicry: Dissecting the ester bond crosslinking mechanics in bacterial adhesin proteins
Protease mimicry: Dissecting the ester bond crosslinking mechanics in bacterial adhesin proteins Open
The ester bond crosslink discovered within bacterial adhesin proteins offers a captivating insight into the convergent evolution of enzyme‐like machinery. Crystal structures reveal a putative catalytic triad comprising an acid–base–nucleop…
View article: Feedback regulation of iron-sulfur cluster biogenesis
Feedback regulation of iron-sulfur cluster biogenesis Open
Iron-sulfur (Fe-S) clusters are ubiquitous cofactors in biological systems. Given their central role in bacterial metabolism and pathogenesis, the biogenesis of Fe-S clusters is tightly controlled. We reveal a feedback regulatory mechanism…
View article: Structures of <i>Mycobacterium tuberculosis</i> isoprenyl diphosphate synthase Rv2173 in substrate-bound forms
Structures of <i>Mycobacterium tuberculosis</i> isoprenyl diphosphate synthase Rv2173 in substrate-bound forms Open
We report structures of the Mycobacterium tuberculosis isoprenyl diphosphate synthase Rv2173 in three forms: apo and two substrate-bound forms [isoprenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)]. The protein possesses a can…
View article: Protease mimicry: dissecting the ester bond crosslinking mechanics in bacterial adhesin proteins
Protease mimicry: dissecting the ester bond crosslinking mechanics in bacterial adhesin proteins Open
The ester bond crosslink discovered within bacterial adhesin proteins offers a captivating insight into the convergent evolution of enzyme-like machinery. Crystal structures reveal a putative catalytic triad comprising an acid-base-nucleop…
View article: Abstract 1821 Investigating the catalytic mechanism of F420-dependent glucose-6-phosphate dehydrogenase through kinetic and thermodynamic analyses
Abstract 1821 Investigating the catalytic mechanism of F420-dependent glucose-6-phosphate dehydrogenase through kinetic and thermodynamic analyses Open
F420-dependent glucose-6-phosphate dehydrogenase (FGD) is an enzyme found in Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis disease. FGD catalyzes the conversion of glucose-6-phosphate (G6P) to 6-phosphogluconolacton…
View article: Abstract 1445 Kinetic Studies on the catalytic mechanism of F420-dependent glucose-6-phosphate dehydrogenase
Abstract 1445 Kinetic Studies on the catalytic mechanism of F420-dependent glucose-6-phosphate dehydrogenase Open
F420- dependent glucose-6-phosphate dehydrogenase (FGD) is an important enzyme found in Mycobacteria tuberculosis, the causative agent of tuberculosis disease. FGD catalyzes the conversion of glucose-6-phosphate (G6P) to 6-phosphogluconola…
View article: Poetry in motion: catching molecules in action
Poetry in motion: catching molecules in action Open
Researchers have long sought to `see' proteins and other macromolecules in motion, to better understand their functions. Technological developments, notably advances in serial crystallography, are now making these dreams a reality, heraldi…
View article: Poly-γ-glutamylation of biomolecules
Poly-γ-glutamylation of biomolecules Open
Poly-γ-glutamate tails are a distinctive feature of archaeal, bacterial, and eukaryotic cofactors, including the folates and F 420 . Despite decades of research, key mechanistic questions remain as to how enzymes successively add glutamate…
View article: Atropine sulfate as a continuous intravenous infusion for the treatment of organophosphate toxicity in a cat
Atropine sulfate as a continuous intravenous infusion for the treatment of organophosphate toxicity in a cat Open
Case summary A 1-year-old male neutered domestic shorthair cat presented on an emergency basis with clinical signs suspected to be secondary to organophosphate (OP) toxicity. The control of clinical abnormalities (bradycardia, obtundation,…
View article: Domain structure and cross-linking in a giant adhesin from the <i>Mobiluncus mulieris</i> bacterium
Domain structure and cross-linking in a giant adhesin from the <i>Mobiluncus mulieris</i> bacterium Open
Cell-surface proteins known as adhesins enable bacteria to colonize particular environments, and in Gram-positive bacteria often contain autocatalytically formed covalent intramolecular cross-links. While investigating the prevalence of su…
View article: <i>AlphaFold</i> and the future of structural biology
<i>AlphaFold</i> and the future of structural biology Open
This editorial acknowledges the transformative impact of new machine-learning methods, such as the use of AlphaFold, but also makes the case for the continuing need for experimental structural biology.
View article: <i>AlphaFold</i> and the future of structural biology
<i>AlphaFold</i> and the future of structural biology Open
This editorial acknowledges the transformative impact of new machine-learning methods, such as the use of AlphaFold, but also makes the case for the continuing need for experimental structural biology.
View article: <i>AlphaFold</i> and the future of structural biology
<i>AlphaFold</i> and the future of structural biology Open
This editorial acknowledges the transformative impact of new machine-learning methods, such as the use of AlphaFold, but also makes the case for the continuing need for experimental structural biology.
View article: Identification of an immunodominant region on a group A <i>Streptococcus</i> T-antigen reveals temperature-dependent motion in pili
Identification of an immunodominant region on a group A <i>Streptococcus</i> T-antigen reveals temperature-dependent motion in pili Open
Group A Streptococcus (GAS) is a globally important pathogen causing a broad range of human diseases. GAS pili are elongated proteins with a backbone comprised repeating T-antigen subunits, which extend from the cell surface and have impor…
View article: Submission of structural biology data for review purposes
Submission of structural biology data for review purposes Open
The editors discuss the submission of structural biology data.
View article: Inhibition of Indigoidine Synthesis as a High-Throughput Colourimetric Screen for Antibiotics Targeting the Essential Mycobacterium tuberculosis Phosphopantetheinyl Transferase PptT
Inhibition of Indigoidine Synthesis as a High-Throughput Colourimetric Screen for Antibiotics Targeting the Essential Mycobacterium tuberculosis Phosphopantetheinyl Transferase PptT Open
A recently-validated and underexplored drug target in Mycobacterium tuberculosis is PptT, an essential phosphopantetheinyl transferase (PPTase) that plays a critical role in activating enzymes for both primary and secondary metabolism. Ppt…
View article: Inhibition of Indigoidine Synthesis as a High-throughput Colourimetric Screen for Antibiotics Targeting the Essential <em>Mycobacterium tuberculosis</em> Phosphopantetheinyl Transferase PptT
Inhibition of Indigoidine Synthesis as a High-throughput Colourimetric Screen for Antibiotics Targeting the Essential <em>Mycobacterium tuberculosis</em> Phosphopantetheinyl Transferase PptT Open
A recently-validated and underexplored drug target in Mycobacterium tuberculosis is PptT, an essential phosphopantetheinyl transferase (PPTase) that plays a critical role in activating enzymes for both primary and secondary metabolism. Ppt…
View article: Some lessons from COVID: science and communication
Some lessons from COVID: science and communication Open
Biomedical challenges such as the present COVID-19 pandemic require both good science and excellent communication between scientists and the general public. This underscores the importance of presenting our science in innovative ways that …
View article: Incentives to Encourage Participation in the National Public Health Accreditation Model: A Systematic Investigation
Incentives to Encourage Participation in the National Public Health Accreditation Model: A Systematic Investigation Open
Objectives. We sought to identify the incentives most likely to encourage voluntary participation in the national public health accreditation model. Methods. We reviewed existing incentives, held meetings with key informants, and conducted…
View article: Informing the National Public Health Accreditation Movement: Lessons From North Carolina's Accredited Local Health Departments
Informing the National Public Health Accreditation Movement: Lessons From North Carolina's Accredited Local Health Departments Open
To advance understanding of public health accreditation, we analyzed data on the North Carolina Local Health Department Accreditation program. We surveyed accredited health departments on barriers to and supports of accreditation preparati…
View article: Tat–Dependent Translocation of an F420–Binding Protein of Mycobacterium tuberculosis
Tat–Dependent Translocation of an F420–Binding Protein of Mycobacterium tuberculosis Open
F420 is a unique cofactor present in a restricted range of microorganisms, including mycobacteria. It has been proposed that F420 has an important role in the oxidoreductive reactions of Mycobacterium tuberculosis, possibly associated with…
View article: Competency development in public health leadership
Competency development in public health leadership Open
The professional development of public health leaders requires competency-based instruction to increase their ability to address complex and changing demands for critical services. This article reviews the development of the Leadership Com…
View article: <b>A Life among Men, Women and Molecules. Memoirs of an Indian Scientist</b>. By MaMannaMana Vijayan. Indian National Science Academy, 2020. Ebook, pp. xi+305. ISBN 978-81-939482-6-2. Price USD 35.00 INR 1500
<b>A Life among Men, Women and Molecules. Memoirs of an Indian Scientist</b>. By MaMannaMana Vijayan. Indian National Science Academy, 2020. Ebook, pp. xi+305. ISBN 978-81-939482-6-2. Price USD 35.00 INR 1500 Open
The role and importance of mentors is a cultural theme that runs through all fields of science.Some years ago, I had the privilege of reviewing a set of three volumes containing the collected works of Dorothy Hodgkin, one of our greatest a…
View article: Visualizing an unseen enemy; mobilizing structural biology to counter COVID-19
Visualizing an unseen enemy; mobilizing structural biology to counter COVID-19 Open
Crystallography in its broadest sense has a crucial role to play in addressing the current COVID-19 pandemic. An outpouring of structural information on key viral proteins has resulted and importantly these data have immediately been share…
View article: Visualizing an unseen enemy; mobilizing structural biology to counter COVID-19
Visualizing an unseen enemy; mobilizing structural biology to counter COVID-19 Open
Crystallography in its broadest sense has a crucial role to play in addressing the current COVID-19 pandemic. An outpouring of structural information on key viral proteins has resulted and importantly these data have immediately been share…