Edwin R. Manuel
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View article: Critical and Comparative Analysis of the 2025 AHA/ACC/AANP/AAPA/ABC/ACCP/ACPM/AGS/AMA/ASPC/NMA/PCNA/SGIM Guideline for Hypertension and Other International Guides and Protocols
Critical and Comparative Analysis of the 2025 AHA/ACC/AANP/AAPA/ABC/ACCP/ACPM/AGS/AMA/ASPC/NMA/PCNA/SGIM Guideline for Hypertension and Other International Guides and Protocols Open
The 2025 AHA/ACC/AANP/AAPA/ABC/ACCP/ACPM/AGS/AMA/ASPC/NMA/PCNA/SGIM guideline for the prevention, detection, evaluation, and management of arterial hypertension introduces important updates compared with the 2017 version. Given the global …
View article: Non-adherence to Drug Therapy for Hypertension is Critical and Underestimated in Mexican Patients: A Study from the First Contact Level
Non-adherence to Drug Therapy for Hypertension is Critical and Underestimated in Mexican Patients: A Study from the First Contact Level Open
Adherence, in a healthcare context, refers to the extent to which a person’s behavior aligns with recommendations from a healthcare provider. This can involve taking medication as prescribed, following a specific diet, or adhering to lifes…
View article: Effects of Hypomethylating Agents on Gene Modulation in the Leukemic Microenvironment and Disease Trajectory in a Mouse Model of AML
Effects of Hypomethylating Agents on Gene Modulation in the Leukemic Microenvironment and Disease Trajectory in a Mouse Model of AML Open
Hypomethylating agents (HMAs), such as decitabine and 5-azacytidine (AZA), are valuable treatment options for patients with acute myeloid leukemia that are ineligible for intensive chemotherapy. Despite providing significant extensions in …
View article: Tumor-Colonizing E. coli Expressing Both Collagenase and Hyaluronidase Enhances Therapeutic Efficacy of Gemcitabine in Pancreatic Cancer Models
Tumor-Colonizing E. coli Expressing Both Collagenase and Hyaluronidase Enhances Therapeutic Efficacy of Gemcitabine in Pancreatic Cancer Models Open
Desmoplasia is a hallmark feature of pancreatic ductal adenocarcinoma (PDAC) that contributes significantly to treatment resistance. Approaches to enhance drug delivery into fibrotic PDAC tumors continue to be an important unmet need. In t…
View article: Tumor-Colonizing <em>E. coli</em> Expressing Both Collagenase and Hyaluronidase Enhances Therapeutic Efficacy of Gemcitabine in Pancreatic Cancer Models
Tumor-Colonizing <em>E. coli</em> Expressing Both Collagenase and Hyaluronidase Enhances Therapeutic Efficacy of Gemcitabine in Pancreatic Cancer Models Open
Desmoplasia is a hallmark feature of pancreatic ductal adenocarcinoma (PDAC) that contributes significantly to treatment resistance. Approaches to enhance drug delivery into fibrotic PDAC tumors continues to be an important unmet need. In …
View article: Supplementary Figure 1 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma
Supplementary Figure 1 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma Open
Supplementary Figure 1 describes BHs expression in uninduced ST strains as well as the predicted localization of BHs. Viability of bHs-expressing ST strains is analyzed.
View article: Supplementary Fig. S9 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S9 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Evaluation of inflammatory cytokines and ICAM-1 in tumors of mice treated with PEGPH20/shIDO-ST. Mice bearing 14 day orthotopic KPC tumors were treated with indicated regimens. Tumors were excised 96 hours post-treatment and used for RT-qP…
View article: Supplementary Figure 4 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma
Supplementary Figure 4 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma Open
Supplementary Figure 4 shows that treatment with X8768-BHs does not degrade HA is non-tumor tissues, and does not affaect total cellular content of tumor tissue where HA was degraded.
View article: Supplementary Fig. S13 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S13 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Isolation and characterization of splenic PMN from PEGPH20/ shIDO-ST treated mice. (A) PMN were purified from crude spleen homogenates of treated mice through negative bead selection. Percentage of PMN in crude and purified preparations we…
View article: Supplementary Fig. S12 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S12 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Western blot analysis of cleaved caspase 3 (CC3). Mice bearing 14 day orthotopic KPC tumors were treated with indicated regimens. Tumors were excised 96 hours post-treatment, homogenized, and total protein was extracted using cell lysis bu…
View article: Supplementary Fig. S8 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S8 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Detection of PMN in extracted tumors of PEGPH20/shIDO-ST treated mice. Mice treated with PEGPH20/shScr-ST or PEGPH20/shIDO-ST were euthanized and tumors extracted 96 hours post-ST treatment. One hour prior to euthanization, mice were injec…
View article: Supplementary Fig. S15 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S15 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Chromium release assay utilizing a range of PMN (effectors) against various tumor targets. Purified splenic PMN from treated mouse groups (n=3) were used in a 51Cr cytotoxicity assay. Tumor targets consisted of KPC (pancreatic cancer), 4T1…
View article: Supplementary Fig. S7 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S7 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
PMN tracking in control mice. (A) Normal C57BL/6 non-tumor bearing mice were injected intravenously with 50 uL of a 20 uM working solution of NS-NIR reagent. Mice were imaged 1 hr proceeding injection of NS-NIR. Mice were also injected wit…
View article: Supplementary Figure 2 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma
Supplementary Figure 2 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma Open
Supplementary Figure 2 describes the ability of X8768-BHs to degrade HA from fixed human PC-3 prostate cancer cells.
View article: Supplementary Fig. S5 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S5 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Colony forming unit (CFU) enumeration of ST in tumors of mice treated with and without PEGPH20. Mice with 14-day orthotopic KPC tumors (n=3) were given indicated treatment regimens. Tumors were then extracted at indicated time points post-…
View article: Supplementary Fig. S7 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S7 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
PMN tracking in control mice. (A) Normal C57BL/6 non-tumor bearing mice were injected intravenously with 50 uL of a 20 uM working solution of NS-NIR reagent. Mice were imaged 1 hr proceeding injection of NS-NIR. Mice were also injected wit…
View article: Supplementary Table 1 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma
Supplementary Table 1 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma Open
Supplementary Table 1 describes the parental strains and mutations used to generate attenuated ST strains.
View article: Supplementary Figure 1 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma
Supplementary Figure 1 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma Open
Supplementary Figure 1 describes BHs expression in uninduced ST strains as well as the predicted localization of BHs. Viability of bHs-expressing ST strains is analyzed.
View article: Supplementary Fig. S1 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S1 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Generation of KPC-luc orthotopic model. (A) 28 day orthotopic KPC tumor (top, 2050 mm3) extracted from untreated C57BL/6 mouse versus normal C57BL/6 pancreas (bottom, 84 mm3). (B) KPC-luc cell line incubated with increasing titration of D-…
View article: Supplementary Fig. S5 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S5 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Colony forming unit (CFU) enumeration of ST in tumors of mice treated with and without PEGPH20. Mice with 14-day orthotopic KPC tumors (n=3) were given indicated treatment regimens. Tumors were then extracted at indicated time points post-…
View article: Supplementary Fig. S8 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S8 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Detection of PMN in extracted tumors of PEGPH20/shIDO-ST treated mice. Mice treated with PEGPH20/shScr-ST or PEGPH20/shIDO-ST were euthanized and tumors extracted 96 hours post-ST treatment. One hour prior to euthanization, mice were injec…
View article: Supplementary Fig. S9 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S9 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Evaluation of inflammatory cytokines and ICAM-1 in tumors of mice treated with PEGPH20/shIDO-ST. Mice bearing 14 day orthotopic KPC tumors were treated with indicated regimens. Tumors were excised 96 hours post-treatment and used for RT-qP…
View article: Supplementary Fig. S10 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S10 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Preliminary testing of PEGPH20/shIDO-ST in autochthonous KPC-Brca1 mouse model. PMN and DAPI immunofluorescence staining of an autochthonous tumor section from a KPC-Brca1 mouse treated with PEGPH20/shIDO-ST. Tumor was excised, flash froze…
View article: Supplementary Fig. S6 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S6 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Ex-vivo depletion of HA from KPC tumor sections with hyaluronidase and PEGPH20. To confirm specificity of HA staining, 14-day orthotopic KPC tumors were extracted, flash frozen in OCT medium, sectioned, and fixed onto Colorfrost slides (Fi…
View article: Supplementary Fig. S1 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S1 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Generation of KPC-luc orthotopic model. (A) 28 day orthotopic KPC tumor (top, 2050 mm3) extracted from untreated C57BL/6 mouse versus normal C57BL/6 pancreas (bottom, 84 mm3). (B) KPC-luc cell line incubated with increasing titration of D-…
View article: Supplementary Figure 2 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma
Supplementary Figure 2 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma Open
Supplementary Figure 2 describes the ability of X8768-BHs to degrade HA from fixed human PC-3 prostate cancer cells.
View article: Supplementary Fig. S6 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S6 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Ex-vivo depletion of HA from KPC tumor sections with hyaluronidase and PEGPH20. To confirm specificity of HA staining, 14-day orthotopic KPC tumors were extracted, flash frozen in OCT medium, sectioned, and fixed onto Colorfrost slides (Fi…
View article: Supplementary Fig. S3 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors
Supplementary Fig. S3 from <i>Salmonella</i>-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Coupled with Enzymatic Depletion of Tumor Hyaluronan Induces Complete Regression of Aggressive Pancreatic Tumors Open
Key replicate treatment groups (A) Replicate IVIS images of key treatment groups emphasizing reproducibility of treatment in KPC orthotopic model. Numbers above images represent days post-tumor implantation. (B) Quantitation of IVIS lucife…
View article: Supplementary Figure 3 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma
Supplementary Figure 3 from Hyaluronidase-Expressing <i>Salmonella</i> Effectively Targets Tumor-Associated Hyaluronic Acid in Pancreatic Ductal Adenocarcinoma Open
Supplementary Figure 3 describes the ability of X8768 expressing bacterial luciferase to colonize tumors and peripheral tissues, as well as how HA degradation affects vasculature in PANC-1 tumors.