Eileen Gentleman
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View article: ALS mutations do not alter perineuronal net formation in human stem cell-derived motor neurons
ALS mutations do not alter perineuronal net formation in human stem cell-derived motor neurons Open
Summary Perineuronal nets (PNNs) are extracellular matrix structures that stabilise synaptic inputs and regulate neuronal plasticity. Although PNN dysregulation is observed in several neurological disorders, their relevance to amyotrophic …
View article: Advances in strontium-releasing biomaterials for bone repair
Advances in strontium-releasing biomaterials for bone repair Open
Strontium-containing biomaterials offer promising avenues for enhancing bone repair by leveraging the ion's reported dual anabolic and anti-catabolic effects on bone cells. These biomaterials aim to encourage site-specific bone growth, hea…
View article: Comprehensive analysis of extracellular matrix remodelling via cyclophilin inhibition in human models of alcohol‐related liver fibrosis
Comprehensive analysis of extracellular matrix remodelling via cyclophilin inhibition in human models of alcohol‐related liver fibrosis Open
Background and Purpose Chronic liver disease and hepatic fibrosis constitute a threat to global health. Clinical translation of preclinical research has been limited, highlighting an urgent need for novel treatments. Cyclophilin inhibitors…
View article: Controlled Release of Human Dental Pulp Stem Cell‐Derived Exosomes from Hydrogels Attenuates Temporomandibular Joint Osteoarthritis
Controlled Release of Human Dental Pulp Stem Cell‐Derived Exosomes from Hydrogels Attenuates Temporomandibular Joint Osteoarthritis Open
Temporomandibular joint osteoarthritis (TMJOA) is a painful inflammatory condition that limits mouth opening. Cell‐derived exosomes, which have anti‐inflammatory effects, are emerging as a treatment for TMJOA. Injection of dental pulp stem…
View article: Morphogen-driven differentiation is precluded by physical confinement in human iPSCs spheroids
Morphogen-driven differentiation is precluded by physical confinement in human iPSCs spheroids Open
Introduction Cell lineage specification is tightly associated with profound morphological changes in the developing human embryo, particularly during gastrulation. The interplay between mechanical forces and biochemical signals is poorly u…
View article: Injectable PEG Hydrogels with Tissue‐Like Viscoelasticity Formed through Reversible Alendronate–Calcium Phosphate Crosslinking for Cell–Material Interactions
Injectable PEG Hydrogels with Tissue‐Like Viscoelasticity Formed through Reversible Alendronate–Calcium Phosphate Crosslinking for Cell–Material Interactions Open
Synthetic hydrogels provide controllable 3D environments, which can be used to study fundamental biological phenomena. The growing body of evidence that cell behavior depends upon hydrogel stress relaxation creates a high demand for hydrog…
View article: FRET Sensor‐Modified Synthetic Hydrogels for Real‐Time Monitoring of Cell‐Derived Matrix Metalloproteinase Activity using Fluorescence Lifetime Imaging
FRET Sensor‐Modified Synthetic Hydrogels for Real‐Time Monitoring of Cell‐Derived Matrix Metalloproteinase Activity using Fluorescence Lifetime Imaging Open
Matrix remodeling plays central roles in a range of physiological and pathological processes and is driven predominantly by the activity of matrix metalloproteinases (MMPs), which degrade extracellular matrix (ECM) proteins. How MMPs regul…
View article: DOP34 Deciphering immune-epithelial interactions in health and in Inflammatory Bowel Disease
DOP34 Deciphering immune-epithelial interactions in health and in Inflammatory Bowel Disease Open
Background Intestinal homeostasis is dependent on appropriate interactions between various compartments including immune, mesenchymal, neural, epithelial and bacteria cells. Disrupt of these interactions has been associated with the develo…
View article: Listen to Your Gut: Key Concepts for Bioengineering Advanced Models of the Intestine
Listen to Your Gut: Key Concepts for Bioengineering Advanced Models of the Intestine Open
The intestine performs functions central to human health by breaking down food and absorbing nutrients while maintaining a selective barrier against the intestinal microbiome. Key to this barrier function are the combined efforts of lumen‐…
View article: Label-free 3-D molecular imaging of living tissues using Raman Spectral Projection Tomography
Label-free 3-D molecular imaging of living tissues using Raman Spectral Projection Tomography Open
The ability to image tissues in three-dimensions (3-D) with label-free molecular contrast at mesoscale would be a valuable capability in biology and biomedicine. Here, we introduce Raman spectral projection tomography (RSPT) for volumetric…
View article: Morphogen-driven human iPSCs differentiation in 3D <i>in vitro</i> models of gastrulation is precluded by physical confinement
Morphogen-driven human iPSCs differentiation in 3D <i>in vitro</i> models of gastrulation is precluded by physical confinement Open
In early human development, gastrulation is tightly associated with lineage specification. The interplay between mechanical forces and biochemical signals during these processes is poorly understood. Here, we dissect the effects of biochem…
View article: RGD density along with substrate stiffness regulate hPSC hepatocyte functionality through YAP signalling
RGD density along with substrate stiffness regulate hPSC hepatocyte functionality through YAP signalling Open
Human pluripotent stem cell-derived hepatocytes (hPSC-Heps) may be suitable for treating liver diseases, but differentiation protocols often fail to yield adult-like cells. We hypothesised that replicating healthy liver niche biochemical a…
View article: Editorial: Extracellular matrix dynamics in biology, bioengineering, and pathology, volume II
Editorial: Extracellular matrix dynamics in biology, bioengineering, and pathology, volume II Open
EDITORIAL article Front. Cell Dev. Biol., 02 December 2022Sec. Cell Adhesion and Migration Volume 10 - 2022 | https://doi.org/10.3389/fcell.2022.1105566
View article: Organoids capture tissue-specific innate lymphoid cell development in mice and humans
Organoids capture tissue-specific innate lymphoid cell development in mice and humans Open
Organoid-based models of murine and human innate lymphoid cell precursor (ILCP) maturation are presented. First, murine intestinal and pulmonary organoids are harnessed to demonstrate that the epithelial niche is sufficient to drive tissue…
View article: Local depletion of proteoglycans mediates cartilage tissue repair in an ex vivo integration model
Local depletion of proteoglycans mediates cartilage tissue repair in an ex vivo integration model Open
Successfully replacing damaged cartilage with tissue-engineered constructs requires integration with the host tissue and could benefit from leveraging the native tissue's intrinsic healing capacity; however, efforts are limited by a poor u…
View article: Intrinsic Mechanical Cues and Their Impact on Stem Cells and Embryogenesis
Intrinsic Mechanical Cues and Their Impact on Stem Cells and Embryogenesis Open
Although understanding how soluble cues direct cellular processes revolutionised the study of cell biology in the second half of the 20th century, over the last two decades, new insights into how mechanical cues similarly impact cell fate …
View article: GSK3 Inhibitor-Induced Dentinogenesis Using a Hydrogel
GSK3 Inhibitor-Induced Dentinogenesis Using a Hydrogel Open
Small-molecule drugs targeting glycogen synthase kinase 3 (GSK3) as inhibitors of the protein kinase activity are able to stimulate reparative dentine formation. To develop this approach into a viable clinical treatment for exposed pulp le…
View article: Selectively Cross-Linked Tetra-PEG Hydrogels Provide Control over Mechanical Strength with Minimal Impact on Diffusivity
Selectively Cross-Linked Tetra-PEG Hydrogels Provide Control over Mechanical Strength with Minimal Impact on Diffusivity Open
Synthetic hydrogels formed from poly(ethylene glycol) (PEG) are widely used to study how cells interact with their extracellular matrix. These in vivo-like 3D environments provide a basis for tissue engineering and cell therapies but also …
View article: OP13 Mucosal organoids capture Innate Lymphoid Cells (ILC) tissue-specific development and reveal that Inflammatory Bowel Disease-associated ILC modulate intestinal remodelling
OP13 Mucosal organoids capture Innate Lymphoid Cells (ILC) tissue-specific development and reveal that Inflammatory Bowel Disease-associated ILC modulate intestinal remodelling Open
Background Innate Lymphoid Cells (ILC) develop from Common Lymphoid Precursors in the bone marrow, and ILC precursors (ILCP) migrate to mucosa where they mature, promote homeostasis, and provide a potent, antigen-non-specific sources of cy…
View article: Design considerations for engineering 3D models to study vascular pathologies in vitro
Design considerations for engineering 3D models to study vascular pathologies in vitro Open
Many cardiovascular diseases (CVD) are driven by pathological remodelling of blood vessels, which can lead to aneurysms, myocardial infarction, ischaemia and strokes. Aberrant remodelling is driven by changes in vascular cell behaviours co…
View article: Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells
Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells Open
To coordinate cell fate with changes in spatial organization, stem cells (SCs) require specific and adaptable systems of signal exchange and cell-to-cell communication. Pluripotent embryonic stem cells (ESCs) use cytonemes to pair with tro…
View article: A Hydrogel-Integrated Culture Device to Interrogate T Cell Activation with Physicochemical Cues
A Hydrogel-Integrated Culture Device to Interrogate T Cell Activation with Physicochemical Cues Open
The recent rise of adoptive T cell therapy (ATCT) as a promising cancer immunotherapy has triggered increased interest in therapeutic T cell bioprocessing. T cell activation is a critical processing step and is known to be modulated by phy…
View article: Three-dimensional niche stiffness synergizes with Wnt7a to modulate the extent of satellite cell symmetric self-renewal divisions
Three-dimensional niche stiffness synergizes with Wnt7a to modulate the extent of satellite cell symmetric self-renewal divisions Open
The skeletal muscle stem cell niche transiently stiffens during the tissue repair process, which serves to increase planar-oriented divisions and, when combined with WNT7a, induces symmetric cell division to expand the stem cell pool.