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View article: In Vitro Culture of Avian Primordial Germ Cells: Established Methods and Future Directions
In Vitro Culture of Avian Primordial Germ Cells: Established Methods and Future Directions Open
Primordial germ cells (PGCs) are the unipotent precursors of sperm and ova, responsible for transmitting hereditary information across generations. Their ability to be isolated and cultured in vitro has opened new horizons for avian biotec…
View article: Detection of Development-Specific MicroRNAs in Rabbit Embryos and Culture Media: A Potential Biomarker Approach for Embryo Quality Assessment
Detection of Development-Specific MicroRNAs in Rabbit Embryos and Culture Media: A Potential Biomarker Approach for Embryo Quality Assessment Open
MicroRNAs (miRNAs) are short, non-coding RNA molecules that play a crucial role in regulating various biological processes by influencing post-transcriptional gene expression and gene silencing. Background/Objectives: In this study, rabbit…
View article: Effects of Polyvinyl Chloride (PVC) Microplastic Particles on Gut Microbiota Composition and Health Status in Rabbit Livestock
Effects of Polyvinyl Chloride (PVC) Microplastic Particles on Gut Microbiota Composition and Health Status in Rabbit Livestock Open
The widespread use of polyvinyl chloride (PVC) and its entry into humans and livestock is of serious concern. In our study, we investigated the impact of PVC treatments on physiological, pathological, hormonal, and microbiota changes in fe…
View article: Creating a novel method for chicken primordial germ cell health monitoring using the fluorescent ubiquitination-based cell cycle indicator reporter system
Creating a novel method for chicken primordial germ cell health monitoring using the fluorescent ubiquitination-based cell cycle indicator reporter system Open
The most current in vitro genetic methods, including gene preservation, gene editing and developmental modelling, require a significant number of healthy cells. In poultry species, primordial germ cells (PGCs) are great candidates for all …
View article: Pluripotency-Associated microRNAs in Early Vertebrate Embryos and Stem Cells
Pluripotency-Associated microRNAs in Early Vertebrate Embryos and Stem Cells Open
MicroRNAs (miRNAs), small non-coding RNA molecules, regulate a wide range of critical biological processes, such as proliferation, cell cycle progression, differentiation, survival, and apoptosis, in many cell types. The regulatory functio…
View article: The Effects of Freezing Media on the Characteristics of Male and Female Chicken Primordial Germ Cell Lines
The Effects of Freezing Media on the Characteristics of Male and Female Chicken Primordial Germ Cell Lines Open
Recently, in vitro gene preservation has gained ground thanks to its lower cost and higher stability compared to in vivo techniques. One of the methods that can preserve female-specific W chromosome-linked genes is primordial germ cell (PG…
View article: Complete blood count and selected serum parameters of Venus transgenic rabbits
Complete blood count and selected serum parameters of Venus transgenic rabbits Open
Green fluorescent protein (GFP) transgenic laboratory animals (mice, rats, rabbits etc.) are commonly used in basic research for modelling human diseases, studying organ development, cell transfer during pregnancy or tissue engineering. Th…
View article: Effect of miR-302b MicroRNA Inhibition on Chicken Primordial Germ Cell Proliferation and Apoptosis Rate
Effect of miR-302b MicroRNA Inhibition on Chicken Primordial Germ Cell Proliferation and Apoptosis Rate Open
The primordial germ cells (PGCs) are the precursors for both the oocytes and spermatogonia. Recently, a novel culture system was established for chicken PGCs, isolated from embryonic blood. The possibility of PGC long-term cultivation issu…
View article: Effect of Post-Hatch Heat-Treatment in Heat-Stressed Transylvanian Naked Neck Chicken
Effect of Post-Hatch Heat-Treatment in Heat-Stressed Transylvanian Naked Neck Chicken Open
Although numerous studies reported the effects of heat stress in chickens, it was not investigated in the Transylvanian Naked Neck breed. In our research, Transylvanian Naked Neck chickens, 24 h after hatching, were heat-treated at 38.5 °C…
View article: Successful cryopreservation and regeneration of a partridge colored Hungarian native chicken breed using primordial germ cells
Successful cryopreservation and regeneration of a partridge colored Hungarian native chicken breed using primordial germ cells Open
Primordial germ cells (PGCs) are the precursors of germline cells that generate sperm and ova in adults. Thus, they are promising tools for gene editing and genetic preservation, especially in avian species. In this study, we established s…
View article: Examination the effect of post-hatch heat-treatment and heat-stress in Transylvanian Naked Neck chicken
Examination the effect of post-hatch heat-treatment and heat-stress in Transylvanian Naked Neck chicken Open
Background One of the most critical global problem nowadays is the increased environmental temperature. Agriculture is very susceptible to this adverse effect because the productivity of animals and poultry decreased. Although several stud…
View article: Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells
Pilot study on cardiogenic differentiation capability of rabbit mesenchymal stem cells Open
Cardiovascular diseases are still one of the most common reasons for mortality in humans. Mesenchymal stem cells (MSCs) are preferable in cardiac regeneration cell-based therapies because of their allogeneic and high proliferative potentia…
View article: Investigation of the Guinea fowl and domestic fowl hybrids as potential surrogate hosts for avian cryopreservation programmes
Investigation of the Guinea fowl and domestic fowl hybrids as potential surrogate hosts for avian cryopreservation programmes Open
In the last decade, avian gene preservation research has focused on the use of the early precursors of the reproductive cells, the primordial germ cells (PGCs). This is because avian PGCs have a unique migration route through the vascular …
View article: Indigenous Hungarian chicken breeds as universal recipients for primordial germ cell-based gene conservation / Őshonos magyar tyúkfajták, mint lehetséges univerzális recipiensek az ősivarsejt alapú génmegőrzésben.
Indigenous Hungarian chicken breeds as universal recipients for primordial germ cell-based gene conservation / Őshonos magyar tyúkfajták, mint lehetséges univerzális recipiensek az ősivarsejt alapú génmegőrzésben. Open
A paper produced in the frame of the H2020 project 677353 'Innovative Management of Animal GEnetic resources' This paper explores the possibility of full genome conservation using primordial germ cells (PGCs) in chickens. The primordial ge…
View article: CRISPR/Cas9-Mediated Knock-Out of dUTPase in Mice Leads to Early Embryonic Lethality
CRISPR/Cas9-Mediated Knock-Out of dUTPase in Mice Leads to Early Embryonic Lethality Open
Sanitization of nucleotide pools is essential for genome maintenance. Deoxyuridine 5′-triphosphate nucleotidohydrolase (dUTPase) is a key enzyme in this pathway since it catalyzes the cleavage of 2′-deoxyuridine 5′-triphosphate (dUTP) into…
View article: Enhancement of chicken primordial germ cell in vitro maintenance using an automated cell image analyser
Enhancement of chicken primordial germ cell in vitro maintenance using an automated cell image analyser Open
Primordial germ cells (PGCs) were isolated from blood samples of chicken embryos. We established four PGC lines: two males (FS-ZZ-101, GFP-ZZ-4ZP) and two females (FS-ZW-111, GFP-ZW-5ZP). We could not detect a significant difference in the…
View article: Comparison of the MicroRNA Expression Profiles of Male and Female Avian Primordial Germ Cell Lines
Comparison of the MicroRNA Expression Profiles of Male and Female Avian Primordial Germ Cell Lines Open
Primordial germ cells (PGCs) are the precursors of adult germ cells, and among the embryonic stem-like cells in the bird embryo, only they can transmit the genetic information to the next generation. Despite the wide range of applications,…
View article: Glomerulosclerosis in transgenic rabbits with ubiquitous Venus protein expression
Glomerulosclerosis in transgenic rabbits with ubiquitous Venus protein expression Open
Focal segmental glomerulosclerosis (FSGS) is a potential cause of nephrotic syndrome both in humans and pet mammals. Glomerulopathy was reported earlier in green fluorescent protein (GFP) transgenic (TG) mice, but glomerulosclerosis has no…
View article: CRISPR/Cas9-mediated knock-out of dUTPase in mice leads to early embryonic lethality
CRISPR/Cas9-mediated knock-out of dUTPase in mice leads to early embryonic lethality Open
Sanitization of nucleotide pools is essential for genome maintenance. Among the enzymes significant in this mechanism, deoxyuridine 5′-triphosphate nucleotidohydrolase (dUTPase) performs cleavage of dUTP into dUMP and inorganic pyrophospha…
View article: Secretion of a recombinant protein without a signal peptide by the exocrine glands of transgenic rabbits
Secretion of a recombinant protein without a signal peptide by the exocrine glands of transgenic rabbits Open
Transgenic rabbits carrying mammary gland specific gene constructs are extensively used for excreting recombinant proteins into the milk. Here, we report refined phenotyping of previously generated Venus transposon-carrying transgenic rabb…
View article: Placenta-specific gene manipulation in rabbits
Placenta-specific gene manipulation in rabbits Open
Lentiviral gene constructs can be efficiently and specifically delivered to trophoblast cell lineages in rodents. In vivo genetic manipulation of trophoblast cell lines enables functional and developmental studies in the placenta. In this …