Erica Elisa Ferrandi
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View article: Functional and structural insights into a thermostable (S)-selective amine transaminase and its improved substrate scope by protein engineering
Functional and structural insights into a thermostable (S)-selective amine transaminase and its improved substrate scope by protein engineering Open
A ( S )-selective amine transaminase from a Streptomyces strain, Sbv333-ATA, is a biocatalyst showing both high thermostability with a melting temperature of 85 °C and broad substrate specificity for the amino acceptor. This enzyme was fur…
View article: A small molecule enhances arrestin-3 binding to the β2-adrenergic receptor
A small molecule enhances arrestin-3 binding to the β2-adrenergic receptor Open
Excessive signaling by various GPCRs underlies a variety of human disorders. Suppression of GPCRs by "enhanced" arrestin mutants was proposed as therapy. We hypothesized that GPCR binding of endogenous arrestins can be increased by small m…
View article: Effective Tubulin Degradation by Rationally Designed Proteolysis Targeting Chimeras
Effective Tubulin Degradation by Rationally Designed Proteolysis Targeting Chimeras Open
Proteolysis targeting chimeras (PROTACs) are heterobifunctional molecules that induce the degradation of proteins of interest (POIs) via the ubiquitin-proteasome pathway by recruiting E3 ligases to form a ternary complex with the POI. In t…
View article: Establishment of an efficient one-step enzymatic synthesis of cyclic-2,3-diphosphoglycerate
Establishment of an efficient one-step enzymatic synthesis of cyclic-2,3-diphosphoglycerate Open
Extremolytes – unique compatible solutes produced by extremophiles - protect biological structures like membranes, proteins, and DNA under extreme conditions, including extremes of temperature and osmotic stress. These compounds hold signi…
View article: Functional and structural insights into a thermostable (S)-selective amine transaminase and its improved substrate scope by protein engineering
Functional and structural insights into a thermostable (S)-selective amine transaminase and its improved substrate scope by protein engineering Open
A (S)-selective amine transaminase from a Streptomyces strain, Sbv333-ATA is a biocatalyst showing both high thermostability with a melting temperature of 85oC and broad substrate specificity for the amino acceptor. This enzyme …
View article: Unlocking Lactonase Enzymes as Biocatalysts for the Deracemisation of Chiral γ‐Thiolactones
Unlocking Lactonase Enzymes as Biocatalysts for the Deracemisation of Chiral γ‐Thiolactones Open
Lactonases, a class of metalloenzymes that exhibit catalytic promiscuity, have been extensively studied from a biological perspective, yet their application as biocatalysts remains underexplored. In this study, we disclose the biocatalytic…
View article: Unlocking Lactonase Enzymes as Biocatalysts for the Deracemisation of Chiral γ‐Thiolactones
Unlocking Lactonase Enzymes as Biocatalysts for the Deracemisation of Chiral γ‐Thiolactones Open
Lactonases, a class of metalloenzymes that exhibit catalytic promiscuity, have been extensively studied from a biological perspective, yet their application as biocatalysts remains underexplored. In this study, we disclose the biocatalytic…
View article: From the discovery of novel enzymes to their synthetic application: investigating and harnessing natural biodiversity toward efficient and selective bioprocesses
From the discovery of novel enzymes to their synthetic application: investigating and harnessing natural biodiversity toward efficient and selective bioprocesses Open
View article: Establishment of an efficient one-step enzymatic synthesis of cyclic-2,3-diphosphoglycerate
Establishment of an efficient one-step enzymatic synthesis of cyclic-2,3-diphosphoglycerate Open
Extremolytes – unique compatible solutes produced by extremophiles - protect biological structures like membranes, proteins, and DNA under extreme conditions, including extremes of temperature and osmotic stress. These compounds hold signi…
View article: Biocatalytic synthesis of the non-pungent capsaicinoid olvanil from agri-food waste
Biocatalytic synthesis of the non-pungent capsaicinoid olvanil from agri-food waste Open
A simple biocatalytic two-step process has been optimised to produce the capsaicinoid olvanil, starting from biomass-derived oleic acid and vanillin and using only biobased reagents and catalysts.
View article: Advances and Challenges in the Development of Immobilized Enzymes for Batch and Flow Biocatalyzed Processes
Advances and Challenges in the Development of Immobilized Enzymes for Batch and Flow Biocatalyzed Processes Open
The development of immobilized enzymes both for batch and continuous flow biocatalytic processes has gained significant traction in recent years, driven by the need for cost‐effective and sustainable production methods in the fine chemical…
View article: Two‐Step Biocatalytic Synthesis of (1<i>S</i>)‐Nor(pseudo)ephedrine Catalyzed by Immobilized Enzymes
Two‐Step Biocatalytic Synthesis of (1<i>S</i>)‐Nor(pseudo)ephedrine Catalyzed by Immobilized Enzymes Open
Nor(pseudo)ephedrines [N(P)Es] are naturally occurring compounds showing sympathomimetic activity that have many applications especially in the chemical industry and in the pharmaceutical field. Recently a two‐step biocatalytic cascade for…
View article: Insight into the Stereoselective Synthesis of (1<i>S</i>)‐Nor(pseudo)ephedrine Analogues by a Two‐Steps Biocatalytic Process
Insight into the Stereoselective Synthesis of (1<i>S</i>)‐Nor(pseudo)ephedrine Analogues by a Two‐Steps Biocatalytic Process Open
Nor(pseudo)ephedrines (N(P)Es), vicinal amino alcohols possessing sympathomimetic biological activity, constitute valuable intermediates and chiral building blocks for the organic synthesis of several active pharmaceutical ingredients (API…
View article: Promiscuity and Selectivity of Hydroxysteroid Dehydrogenases in the Biocatalyzed Reduction of 1,2‐Diketones
Promiscuity and Selectivity of Hydroxysteroid Dehydrogenases in the Biocatalyzed Reduction of 1,2‐Diketones Open
Hydroxysteroid dehydrogenases (HSDHs) are NAD(P)H‐dependent alcohol dehydrogenases (ADHs) known for their exceptional stereo‐ and regioselectivity when acting on natural substrates, including neutral steroids, bile acids, and various stero…
View article: Chemoenzymatic approach towards the synthesis of the antitumor and antileishmanial marine metabolite (+)-Harzialactone A via the stereoselective, biocatalyzed reduction of a prochiral ketone
Chemoenzymatic approach towards the synthesis of the antitumor and antileishmanial marine metabolite (+)-Harzialactone A via the stereoselective, biocatalyzed reduction of a prochiral ketone Open
As a rich source of biological active compounds, marine natural products have been increasingly screened as candidates for developing new drugs. Among the several marine products and metabolites, (+)-Harzialactone A has drawn considerable …
View article: Functional Characterization and Synthetic Application of Is2-SDR, a Novel Thermostable and Promiscuous Ketoreductase from a Hot Spring Metagenome
Functional Characterization and Synthetic Application of Is2-SDR, a Novel Thermostable and Promiscuous Ketoreductase from a Hot Spring Metagenome Open
In a metagenome mining-based search of novel thermostable hydroxysteroid dehydrogenases (HSDHs), enzymes that are able to selectively oxidize/reduce steroidal compounds, a novel short-chain dehydrogenase/reductase (SDR), named Is2-SDR, was…
View article: Selective Supercritical CO2 Extraction and Biocatalytic Valorization of Cucurbita pepo L. Industrial Residuals
Selective Supercritical CO2 Extraction and Biocatalytic Valorization of Cucurbita pepo L. Industrial Residuals Open
The valorization of biomass residuals constitutes a key aspect of circular economy and thus a major challenge for the scientific community. Among industrial wastes, plant residuals could represent an attractive source of bioactive compound…
View article: Chemoenzymatic Synthesis of Enantioenriched (<i>R</i>)‐ and (<i>S</i>)‐Aryloxyalkanoic Herbicides
Chemoenzymatic Synthesis of Enantioenriched (<i>R</i>)‐ and (<i>S</i>)‐Aryloxyalkanoic Herbicides Open
The combination of a biocatalytic asymmetric C=C reduction with a simple sequence of chemical transformations was implemented in a new chemoenzymatic synthesis of various substituted aryloxyalkanoic acids, used as weed‐killing agrochemical…
View article: Stereoselective Biocatalyzed Reductions of Ginger Active Components Recovered from Industrial Wastes
Stereoselective Biocatalyzed Reductions of Ginger Active Components Recovered from Industrial Wastes Open
Ginger is among the most widespread and widely consumed traditional medicinal plants around the world. Its beneficial effects, which comprise e. g. anticancer and anti‐inflammatory activities as well as gastrointestinal regulatory effects,…
View article: Synthesis of ω‐Muricholic Acid by One‐Pot Enzymatic Mitsunobu Inversion using Hydroxysteroid Dehydrogenases
Synthesis of ω‐Muricholic Acid by One‐Pot Enzymatic Mitsunobu Inversion using Hydroxysteroid Dehydrogenases Open
The biocatalyzed conversion of hyocholic acid (3α,6α,7α‐trihydroxy‐5β‐cholan‐24‐oic acid) into ω‐muricholic acid (3α,6α,7β‐trihydroxy‐5β‐cholan‐24‐oic acid) has been obtained exploiting a small library of 7α‐ and 7β‐HSDHs (hydroxysteroid d…
View article: Novel Transaminase and Laccase from Streptomyces spp. Using Combined Identification Approaches
Novel Transaminase and Laccase from Streptomyces spp. Using Combined Identification Approaches Open
Three Streptomyces sp. strains with a multitude of target enzymatic activities confirmed by functional screening, namely BV129, BV286 and BV333, were subjected to genome sequencing aiming at the annotation of genes of interest, in-depth bi…
View article: Design, Synthesis and In Vitro Investigation of Novel Basic Celastrol Carboxamides as Bio-Inspired Leishmanicidal Agents Endowed with Inhibitory Activity against Leishmania Hsp90
Design, Synthesis and In Vitro Investigation of Novel Basic Celastrol Carboxamides as Bio-Inspired Leishmanicidal Agents Endowed with Inhibitory Activity against Leishmania Hsp90 Open
The natural triterpene celastrol (CE) is here used as lead compound for the design and synthesis of a panel of eleven CE carboxamides that were tested in vitro for their growth inhibitory activity against Leishmania infantum and L.tropica …
View article: Biocatalysis with Laccases: An Updated Overview
Biocatalysis with Laccases: An Updated Overview Open
Laccases are multicopper oxidases, which have been widely investigated in recent decades thanks to their ability to oxidize organic substrates to the corresponding radicals while producing water at the expense of molecular oxygen. Besides …
View article: Recombinant Oleate Hydratase from Lactobacillus rhamnosus ATCC 53103: Enzyme Expression and Design of a Reliable Experimental Procedure for the Stereoselective Hydration of Oleic Acid
Recombinant Oleate Hydratase from Lactobacillus rhamnosus ATCC 53103: Enzyme Expression and Design of a Reliable Experimental Procedure for the Stereoselective Hydration of Oleic Acid Open
Different microbial strains are able to transform oleic acid (OA) into 10-hydroxystearic acid (10-HSA) by means of the catalytic activity of the enzymes oleate hydratase (EC 4.2.1.53). Lactobacillus rhamnosus ATCC 53103 performs this biotr…
View article: Discovery and Characterization of a Novel Thermostable β‐Amino Acid Transaminase from a <i>Meiothermus</i> Strain Isolated in an Icelandic Hot Spring
Discovery and Characterization of a Novel Thermostable β‐Amino Acid Transaminase from a <i>Meiothermus</i> Strain Isolated in an Icelandic Hot Spring Open
A Meiothermus strain capable of using β‐phenylalanine for growth is isolated by culture enrichment of samples collected in hot environments and the genome is sequenced showing the presence of 22 putative transaminase (TA) sequences. On the…
View article: Perfluorinated Probes for Noncovalent Protein Recognition and Isolation
Perfluorinated Probes for Noncovalent Protein Recognition and Isolation Open
Perfluorinated organic compounds (PFCs) are nontoxic, biocompatible, bioavailable, and bioorthogonal species which possess the unique ability to segregate away from both polar and nonpolar solvents producing a compact fluorophilic phase. T…
View article: Use of Immobilized Amine Transaminase from <i>Vibrio fluvialis</i> under Flow Conditions for the Synthesis of (<i>S</i>)‐1‐(5‐Fluoropyrimidin‐2‐yl)‐ethanamine
Use of Immobilized Amine Transaminase from <i>Vibrio fluvialis</i> under Flow Conditions for the Synthesis of (<i>S</i>)‐1‐(5‐Fluoropyrimidin‐2‐yl)‐ethanamine Open
We report on the covalent immobilization of the ( S )‐selective amine transaminase from Vibrio fluvialis ( Vf ‐ATA) and its use in the synthesis of ( S )‐1‐(5‐fluoropyrimidin‐2‐yl)‐ethanamine, a key intermediate of the JAK2 kinase inhibito…
View article: Glycosidase-catalyzed synthesis of glycosylated nutraceutical ingredients
Glycosidase-catalyzed synthesis of glycosylated nutraceutical ingredients Open
View article: Target-Oriented Development Of Novel Antiprotozoal Agents: Celastrol Carboxamides As Inhibitors Of Leishmania Hsp90
Target-Oriented Development Of Novel Antiprotozoal Agents: Celastrol Carboxamides As Inhibitors Of Leishmania Hsp90 Open
View article: Stereoselectivity Switch in the Reduction of α-Alkyl-β-Arylenones by Structure-Guided Designed Variants of the Ene Reductase OYE1
Stereoselectivity Switch in the Reduction of α-Alkyl-β-Arylenones by Structure-Guided Designed Variants of the Ene Reductase OYE1 Open
Ene reductases from the Old Yellow Enzyme (OYE) family are industrially interesting enzymes for the biocatalytic asymmetric reduction of alkenes. To access both enantiomers of the target reduced products, stereocomplementary pairs of OYE e…