Ellen Bushell
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View article: A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite <i>Plasmodium berghei</i>
A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite <i>Plasmodium berghei</i> Open
Many Plasmodium genes remain uncharacterized due to low genetic tractability. Previous large-scale knockout screens have only been able to target about half of the genome in the more genetically tractable rodent malaria parasite Plasmodium…
View article: Application of barcode sequencing to increase the throughput and complexity of<i>Plasmodium falciparum</i>genetic screening
Application of barcode sequencing to increase the throughput and complexity of<i>Plasmodium falciparum</i>genetic screening Open
Summary All the pathology and symptoms associated with malaria are caused by the growth of Plasmodium parasites inside human red blood cells. This process, which in the case of the major human malaria pathogen Plasmodium falciparum takes p…
View article: A novel chemogenomic screening platform for scalable antimalarial drug target identification
A novel chemogenomic screening platform for scalable antimalarial drug target identification Open
Summary Large-scale chemical-genetic screening, or chemogenomics, can faciliate rapid and scalable drug target identification. To establish a chemogenomic screen for antimalarial drug target identification, we leveraged ∼600 Plasmodium ber…
View article: Erythrocyte membrane protein 3 (EMAP3) is exposed on the surface of the<i>Plasmodium berghei</i>infected red blood cell
Erythrocyte membrane protein 3 (EMAP3) is exposed on the surface of the<i>Plasmodium berghei</i>infected red blood cell Open
The human malaria parasite Plasmodium falciparum invades red blood cells (RBC) and exports parasite proteins to transform the host cell for its survival. These exported proteins facilitate uptake of nutrients and cytoadherence of the infec…
View article: A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite<i>Plasmodium berghei</i>
A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite<i>Plasmodium berghei</i> Open
Many Plasmodium genes remain uncharacterised due to low genetic tractability. Previous large scale knockout screens have only been able to target about half of the genome in the more genetically tractable rodent malaria parasite Plasmodium…
View article: CRISPR/Cas9 and genetic screens in malaria parasites: small genomes, big impact
CRISPR/Cas9 and genetic screens in malaria parasites: small genomes, big impact Open
The ∼30 Mb genomes of the Plasmodium parasites that cause malaria each encode ∼5000 genes, but the functions of the majority remain unknown. This is due to a paucity of functional annotation from sequence homology, which is compounded by l…
View article: Regulators of male and female sexual development critical for transmission of a malaria parasite
Regulators of male and female sexual development critical for transmission of a malaria parasite Open
Summary The transmission of malaria parasites from vertebrate host to mosquito vector requires a developmental switch in asexually dividing blood-stage parasites to sexual reproduction. In Plasmodium berghei the transcription factor AP2-G …
View article: An enhanced toolkit for the generation of knockout and marker-free fluorescent Plasmodium chabaudi
An enhanced toolkit for the generation of knockout and marker-free fluorescent Plasmodium chabaudi Open
The rodent parasite Plasmodium chabaudi is an important in vivo model of malaria. The ability to produce chronic infections makes it particularly useful for investigating the development of anti-Plasmodium immunity, as well as features ass…
View article: An enhanced toolkit for the generation of knockout and marker-free fluorescent Plasmodium chabaudi
An enhanced toolkit for the generation of knockout and marker-free fluorescent Plasmodium chabaudi Open
The rodent parasite Plasmodium chabaudi is an important in vivo model of malaria. The ability to produce chronic infections makes it particularly useful for investigating the development of anti-Plasmodium immunity, as well as features ass…
View article: Landscape of the Plasmodium Interactome Reveals Both Conserved and Species-Specific Functionality
Landscape of the Plasmodium Interactome Reveals Both Conserved and Species-Specific Functionality Open
Malaria represents a major global health issue, and the identification of new intervention targets remains an urgent priority. This search is hampered by more than one-third of the genes of malaria-causing Plasmodium parasites being unchar…
View article: Complete avian malaria parasite genomes reveal features associated with lineage-specific evolution in birds and mammals
Complete avian malaria parasite genomes reveal features associated with lineage-specific evolution in birds and mammals Open
Avian malaria parasites are prevalent around the world and infect a wide diversity of bird species. Here, we report the sequencing and analysis of high-quality draft genome sequences for two avian malaria species, Plasmodium relictum and P…