Ellen M. Dotson
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View article: An evaluation of longitudinal<i>Anopheles stephensi</i>egg viability and resistance to desiccation over time
An evaluation of longitudinal<i>Anopheles stephensi</i>egg viability and resistance to desiccation over time Open
Anopheles stephensi , a malaria vector in South Asia and parts of the Middle East, has been detected as an invasive species in numerous African countries in recent years. It threatens to increase malaria disease burden and reverse gains ma…
View article: Bicarbonate, calcium ions, hydrogen peroxide and trypsin modulate activation of Anopheles gambiae sperm motility and protein tyrosine phosphorylation
Bicarbonate, calcium ions, hydrogen peroxide and trypsin modulate activation of Anopheles gambiae sperm motility and protein tyrosine phosphorylation Open
With the increasing concern of potential loss of transgenic mosquitoes which are candidates as new tools for mosquito-borne disease control, methods for cryopreservation are actively under investigation. Methods to cryopreserve Anopheles g…
View article: Juvenile Hormone as a contributing factor in establishing midgut microbiota for fecundity and fitness enhancement in adult female Aedes aegypti
Juvenile Hormone as a contributing factor in establishing midgut microbiota for fecundity and fitness enhancement in adult female Aedes aegypti Open
Understanding the factors influencing mosquitoes’ fecundity and longevity is important for designing better and more sustainable vector control strategies, as these parameters can impact their vectorial capacity. Here, we address how matin…
View article: Distribution and dynamics of Anopheles gambiae s.l. larval habitats in three Senegalese cities with high urban malaria incidence
Distribution and dynamics of Anopheles gambiae s.l. larval habitats in three Senegalese cities with high urban malaria incidence Open
Urban malaria has become a challenge for most African countries due to urbanization, with increasing population sizes, overcrowding, and movement into cities from rural localities. The rapid expansion of cities with inappropriate water dra…
View article: Urban malaria vector bionomics and human sleeping behavior in three cities in Senegal
Urban malaria vector bionomics and human sleeping behavior in three cities in Senegal Open
Background Malaria is endemic in Senegal, with seasonal transmission, and the entire population is at risk. In recent years, high malaria incidence has been reported in urban and peri-urban areas of Senegal. An urban landscape analysis was…
View article: Aedes aegypti midgut microbiota establishment in adult females is regulated by Juvenile Hormone to enhance fecundity and fitness.
Aedes aegypti midgut microbiota establishment in adult females is regulated by Juvenile Hormone to enhance fecundity and fitness. Open
Understanding the physiological phenomena involved in female mosquitoes' preparation for a blood meal, including the effects of mating, is crucial for enhancing vector-control strategies against mosquito-borne diseases. At the same time, t…
View article: <i>Anopheles</i>Egg Collection, Disinfection, and Hatching
<i>Anopheles</i>Egg Collection, Disinfection, and Hatching Open
Gravid (i.e., with fully developed eggs), mated Anopheles females typically lay their eggs directly on water ∼48–72 h after a blood meal. Unlike some other mosquito species, Anopheles eggs cannot be desiccated and stored for long durations…
View article: Assessment of Bio-Based Materials as a Sustainable and Scalable Alternative for Detection of <i>Plasmodium</i> spp. (Haemospororida: Plasmodiidae) Sporozoites in Field Deployable Testing
Assessment of Bio-Based Materials as a Sustainable and Scalable Alternative for Detection of <i>Plasmodium</i> spp. (Haemospororida: Plasmodiidae) Sporozoites in Field Deployable Testing Open
Malaria is responsible for over 435,000 deaths annually, mostly occurring in sub-Saharan Africa. Detecting Plasmodium spp. sporozoites (spzs) in the salivary glands of Anopheles (Diptera: Culicidae) vectors with circumsporozoite enzyme-lin…
View article: Countrywide insecticide resistance monitoring and first report of the presence of knock down resistance east in Niger, West Africa
Countrywide insecticide resistance monitoring and first report of the presence of knock down resistance east in Niger, West Africa Open
Background Mass distribution of insecticide treated nets (ITNs) is the principal malaria vector control strategy adopted by Niger. To better inform on the most appropriate ITNs to distribute, the Niger National Malaria Control Programme (N…
View article: Countrywide insecticide resistance monitoring and first report of the presence of knock down resistance east in Niger, West Africa
Countrywide insecticide resistance monitoring and first report of the presence of knock down resistance east in Niger, West Africa Open
Background Mass distribution of insecticide treated nets (ITNs) is the principal malaria vector control strategy adopted by Niger. To better inform on the most appropriate ITNs to distribute, the Niger National Malaria Control Programme (N…
View article: Evaluation of the residual efficacy and physical durability of five long-lasting insecticide- treated nets (LLINs) in Senegal
Evaluation of the residual efficacy and physical durability of five long-lasting insecticide- treated nets (LLINs) in Senegal Open
Background The preventive and curative strategies of malaria are based on promoting the use of long-lasting insecticidal bed nets (LLINs) and treating confirmed cases with artemisinin-based combination therapies. These strategies have led …
View article: Effective Oral RNA Interference (RNAi) Administration to Adult <em>Anopheles gambiae</em> Mosquitoes
Effective Oral RNA Interference (RNAi) Administration to Adult <em>Anopheles gambiae</em> Mosquitoes Open
RNA interference has been a heavily utilized tool for reverse genetic analysis for two decades. In adult mosquitoes, double-stranded RNA (dsRNA) administration has been accomplished primarily via injection, which requires significant time …
View article: Assessment of bio-based materials for enhanced signal detection of sporozoites using csELISA
Assessment of bio-based materials for enhanced signal detection of sporozoites using csELISA Open
BackgroundMalaria is responsible for over 435,000 deaths annually, with most cases occurring in sub-Saharan Africa. Detecting the presence of Plasmodium spp. sporozoites (spzs) in the salivary glands of Anopheles vectors of the parasites u…
View article: Adaptation of ELISA detection of Plasmodium falciparum and Plasmodium vivax circumsporozoite proteins in mosquitoes to a multiplex bead-based immunoassay
Adaptation of ELISA detection of Plasmodium falciparum and Plasmodium vivax circumsporozoite proteins in mosquitoes to a multiplex bead-based immunoassay Open
Background Plasmodium spp. sporozoite rates in mosquitoes are used to better understand malaria transmission intensity, the relative importance of vector species and the impact of interventions. These rates are typically estimated using an…
View article: Evidence supporting deployment of next generation insecticide treated nets in Burkina Faso: bioassays with either chlorfenapyr or piperonyl butoxide increase mortality of pyrethroid-resistant Anopheles gambiae s.l.
Evidence supporting deployment of next generation insecticide treated nets in Burkina Faso: bioassays with either chlorfenapyr or piperonyl butoxide increase mortality of pyrethroid-resistant Anopheles gambiae s.l. Open
Background Pyrethroid resistance poses a major threat to the efficacy of insecticide treated nets (ITNs) in Burkina Faso and throughout sub-Saharan Africa, particularly when resistance is present at high intensity. For such areas there are…
View article: Adaptation of ELISA Detection of Plasmodium Falciparum and Plasmodium Vivax Circumsporozoite Proteins in Mosquitoes to a Multiplex Bead-Based Immunoassay
Adaptation of ELISA Detection of Plasmodium Falciparum and Plasmodium Vivax Circumsporozoite Proteins in Mosquitoes to a Multiplex Bead-Based Immunoassay Open
Background: Plasmodium spp. sporozoite rates in mosquitoes are used to better understand malaria transmission intensity, the relative importance of vector species and the impact of interventions. These rates are typically estimated using a…
View article: Additional file 3 of Evidence supporting deployment of next generation insecticide treated nets in Burkina Faso: bioassays with either chlorfenapyr or piperonyl butoxide increase mortality of pyrethroid-resistant Anopheles gambiae
Additional file 3 of Evidence supporting deployment of next generation insecticide treated nets in Burkina Faso: bioassays with either chlorfenapyr or piperonyl butoxide increase mortality of pyrethroid-resistant Anopheles gambiae Open
Additional file 3. Synergist assays using pyrethroid insecticides and piperonyl butoxide data.
View article: Additional file 2 of Evidence supporting deployment of next generation insecticide treated nets in Burkina Faso: bioassays with either chlorfenapyr or piperonyl butoxide increase mortality of pyrethroid-resistant Anopheles gambiae
Additional file 2 of Evidence supporting deployment of next generation insecticide treated nets in Burkina Faso: bioassays with either chlorfenapyr or piperonyl butoxide increase mortality of pyrethroid-resistant Anopheles gambiae Open
Additional file 2. Temperature and humidity conditions of WHO susceptibility and CDC bottle bioassays.
View article: Additional file 4 of Evidence supporting deployment of next generation insecticide treated nets in Burkina Faso: bioassays with either chlorfenapyr or piperonyl butoxide increase mortality of pyrethroid-resistant Anopheles gambiae
Additional file 4 of Evidence supporting deployment of next generation insecticide treated nets in Burkina Faso: bioassays with either chlorfenapyr or piperonyl butoxide increase mortality of pyrethroid-resistant Anopheles gambiae Open
Additional file 4. CDC bottle assays using 100ug chlorfenapyr data.
View article: Cryoprotectant toxicity and hypothermic sensitivity among Anopheles larvae
Cryoprotectant toxicity and hypothermic sensitivity among Anopheles larvae Open
Laboratory rearing of mosquitoes is commonly practiced by researchers studying mosquito-borne infectious diseases and vector control methods. In the absence of cryopreservation methods to stabilize unique or genetically modified strains, m…
View article: Delimiting cryptic morphological variation among human malaria vector species using convolutional neural networks
Delimiting cryptic morphological variation among human malaria vector species using convolutional neural networks Open
Deep learning is a powerful approach for distinguishing classes of images, and there is a growing interest in applying these methods to delimit species, particularly in the identification of mosquito vectors. Visual identification of mosqu…
View article: Physiological responses to cryoprotectant treatment in an early larval stage of the malaria mosquito, Anopheles gambiae
Physiological responses to cryoprotectant treatment in an early larval stage of the malaria mosquito, Anopheles gambiae Open
The development of cryopreservation protocols for Anopheles gambiae could significantly improve research and control efforts. Cryopreservation of any An. gambiae life stage has yet to be successful. The unique properties of embryos have pr…
View article: Oral Delivery of dsRNA Targeting a Female-Biased Flight Muscle Actin Impairs Flight in the Malaria Vector, Anopheles Albimanus.
Oral Delivery of dsRNA Targeting a Female-Biased Flight Muscle Actin Impairs Flight in the Malaria Vector, Anopheles Albimanus. Open
BackgroundDespite the progress to eliminate malaria in Central America, focalized transmission persists, and insecticide resistance is on the rise in the primary vector, Anopheles albimanus . Many of the new control methods being developed…
View article: Trials of the Automated Particle Counter for laboratory rearing of mosquito larvae
Trials of the Automated Particle Counter for laboratory rearing of mosquito larvae Open
As a means of obtaining reproducible and accurate numbers of larvae for laboratory rearing, we tested a large-particle flow-cytometer type device called the ‘Automated Particle Counter’ (APC). The APC is a gravity-fed, self-contained unit …