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View article: A multiplex targeted NGS panel for identifying pathogens in canine neurological and reproductive diseases
A multiplex targeted NGS panel for identifying pathogens in canine neurological and reproductive diseases Open
Introduction Multiple pathogens can infect the canine reproductive and central nervous systems, including organisms that are zoonotic, such as Brucella canis , pathogenic Leptospira spp., Anaplasma phagocytophilum , Histoplasma capsulatum …
View article: An Enigmatic Wild Passerine Mortality Event in the Eastern United States
An Enigmatic Wild Passerine Mortality Event in the Eastern United States Open
The ability to rapidly respond to wildlife health events is essential. However, such events are often unpredictable, especially with anthropogenic disturbances and climate-related environmental changes driving unforeseen threats. Many even…
View article: Feline ocular and respiratory infections: a retrospective analysis of clinical cases submitted to Georgia Veterinary Diagnostic Laboratories (2012–2022)
Feline ocular and respiratory infections: a retrospective analysis of clinical cases submitted to Georgia Veterinary Diagnostic Laboratories (2012–2022) Open
Objectives The objective of this study was to retrospectively assess the pathogens associated with feline ocular and respiratory diseases in routine diagnostic samples submitted to Georgia Veterinary Diagnostic Laboratories. Furthermore, p…
View article: Surveillance of Highly Pathogenic Avian Influenza Virus in Wild Canids from Pennsylvania, USA
Surveillance of Highly Pathogenic Avian Influenza Virus in Wild Canids from Pennsylvania, USA Open
The avian influenza virus is a global pathogen with significant health and economic implications. While primarily a pathogen of wild and domestic birds, recent outbreaks of the H5N1 highly pathogenic avian influenza virus (HPAIV) clade 2.3…
View article: METAGENOMICS FOR PATHOGEN DETECTION DURING A MASS MORTALITY EVENT IN SONGBIRDS
METAGENOMICS FOR PATHOGEN DETECTION DURING A MASS MORTALITY EVENT IN SONGBIRDS Open
Mass mortality events in wildlife can be indications of an emerging infectious disease. During the spring and summer of 2021, hundreds of dead passerines were reported across the eastern US. Birds exhibited a range of clinical signs includ…
View article: Moraxella oculi sp. nov., isolated from a cow with infectious bovine keratoconjunctivitis
Moraxella oculi sp. nov., isolated from a cow with infectious bovine keratoconjunctivitis Open
A novel species of the genus Moraxella was isolated from an ocular swab from a cow with infectious bovine keratoconjunctivitis. 16S rRNA gene sequencing suggested this species was Moraxella bovis (99.59 % nucleotide identity). Average nucl…
View article: Targeted next-generation sequencing assay to detect 3 <i>Moraxella</i> spp. directly from bovine ocular swabs
Targeted next-generation sequencing assay to detect 3 <i>Moraxella</i> spp. directly from bovine ocular swabs Open
Infectious bovine keratoconjunctivitis (IBK) is associated with 2 species of Moraxella: M. bovis and M. bovoculi. A third novel Moraxella spp., designated tentatively as M. oculi, has been identified from the eyes of cattle with and withou…
View article: Transfer of naturally acquired specific passive immunity against<i>Anaplasma phagocytophilum</i>in foals in Southeastern Pennsylvania and Northern Maryland
Transfer of naturally acquired specific passive immunity against<i>Anaplasma phagocytophilum</i>in foals in Southeastern Pennsylvania and Northern Maryland Open
Background Equine granulocytic anaplasmosis (EGA) is a common disease in adult horses, but clinical disease in foals is rarely reported. The relationship between equine maternal and neonatal antibodies to Anaplasma phagocytophilum is uncle…
View article: Metagenomics for pathogen detection during a wildlife mortality event in songbirds
Metagenomics for pathogen detection during a wildlife mortality event in songbirds Open
Mass mortality events in wildlife can be indications of an emerging infectious disease. During the spring and summer of 2021, hundreds of dead passerines were reported across the eastern US. Birds exhibited a range of clinical signs includ…
View article: Investigation of the pathogens contributing to naturally occurring outbreaks of infectious bovine keratoconjunctivitis (pinkeye) using Next Generation Sequencing
Investigation of the pathogens contributing to naturally occurring outbreaks of infectious bovine keratoconjunctivitis (pinkeye) using Next Generation Sequencing Open
Infectious bovine keratoconjunctivitis (IBK), commonly known as pinkeye, has a marked negative impact on the economy of the cattle industry. Moraxella species, including Mor. bovis and Mor. bovoculi, which have been associated with this di…
View article: Environmental control reduces white‐nose syndrome infection in hibernating bats
Environmental control reduces white‐nose syndrome infection in hibernating bats Open
Infectious diseases caused by invasive, environmentally persistent fungal pathogens have increasingly endangered global biodiversity, yet disease management remains a major conservation challenge. A prominent example is white‐nose syndrome…
View article: Multiple Introductions of SARS-CoV-2 Alpha and Delta Variants into White-Tailed Deer in Pennsylvania
Multiple Introductions of SARS-CoV-2 Alpha and Delta Variants into White-Tailed Deer in Pennsylvania Open
Coronaviruses have been documented to replicate in numerous species of vertebrates, and multiple spillovers of coronaviruses from animals into humans have founded human epidemics. The COVID-19 epidemic likely derived from a spillover of SA…
View article: Bat Red Blood Cells Express Nucleic Acid–Sensing Receptors and Bind RNA and DNA
Bat Red Blood Cells Express Nucleic Acid–Sensing Receptors and Bind RNA and DNA Open
RBCs demonstrate immunomodulatory capabilities through the expression of nucleic acid sensors. However, little is known about bat RBCs, and no studies have examined the immune function of bat erythrocytes. In this study, we show that bat R…
View article: Multiple Introductions of SARS-CoV-2 Alpha and Delta Variants into White-Tailed Deer in Pennsylvania
Multiple Introductions of SARS-CoV-2 Alpha and Delta Variants into White-Tailed Deer in Pennsylvania Open
Introductory paragraph The SARS-CoV-2 pandemic likely began by viral spillover from animals to humans 1-3 ; today multiple animal species are known to be susceptible to infection 4-8 . White-tailed deer, Odocoileus virginianus are infected…
View article: Bat Red Blood Cells express Nucleic Acid Sensing Receptors and bind RNA and DNA
Bat Red Blood Cells express Nucleic Acid Sensing Receptors and bind RNA and DNA Open
Red blood cells (RBCs) demonstrate immunomodulatory capabilities through the expression of nucleic acid sensors. However, little is known about bat RBCs, and no studies have examined the immune function of bat erythrocytes. Here we show th…
View article: Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip
Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip Open
Infectious laryngotracheitis (ILT) is a viral disease of chickens’ respiratory system that imposes considerable financial burdens on the chicken industry. Rapid, simple, and specific detection of this virus is crucial to enable proper cont…
View article: Performance of commercial PCR assays to detect toxigenic <i>Clostridioides difficile</i> in the feces of puppies
Performance of commercial PCR assays to detect toxigenic <i>Clostridioides difficile</i> in the feces of puppies Open
Clostridioides difficile is an important enteric pathogen that causes significant morbidity and mortality in humans. With community‐acquired infections on the rise, it is important to identify reservoirs of the pathogen. Companion animals …
View article: Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers
Differences in the genome, methylome, and transcriptome do not differentiate isolates of Streptococcus equi subsp. equi from horses with acute clinical signs from isolates of inapparent carriers Open
Streptococcus equi subsp. equi (SEE) is a host-restricted bacterium that causes the common infectious upper respiratory disease known as strangles in horses. Perpetuation of SEE infection appears attributable to inapparent carrier horses b…
View article: Evaluation of a real-time RT-PCR panel for detection of SARS-CoV-2 in bat guano
Evaluation of a real-time RT-PCR panel for detection of SARS-CoV-2 in bat guano Open
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease 2019 (COVID-19), which is an ongoing global health concern. The exact source of the virus has not been identified, but it is believe…
View article: Two stage, nested isothermal amplification in a single tube
Two stage, nested isothermal amplification in a single tube Open
Sensitive, specific and rapid molecular diagnosis of respiratory diseases in animals and humans is critical to facilitate appropriate control measures and treatment.
View article: Evaluation of targeted next-generation sequencing for detection of equine pathogens in clinical samples
Evaluation of targeted next-generation sequencing for detection of equine pathogens in clinical samples Open
Equine infectious disease outbreaks may have profound economic impact, resulting in losses of millions of dollars of revenue as a result of horse loss, quarantine, and cancelled events. Early and accurate diagnosis is essential to limit th…
View article: Concurrent Infection of Skunk Adenovirus-1, Listeria monocytogenes, and a Regionally Specific Clade of Canine Distemper Virus in One Gray Fox (Urocyon cinereoargenteus) and Concurrent Listeriosis and Canine Distemper in a Second Gray Fox
Concurrent Infection of Skunk Adenovirus-1, Listeria monocytogenes, and a Regionally Specific Clade of Canine Distemper Virus in One Gray Fox (Urocyon cinereoargenteus) and Concurrent Listeriosis and Canine Distemper in a Second Gray Fox Open
One free-ranging Gray fox (Urocyon cinereoargenteus) underwent autopsy following neurologic disease, with findings including morbilliviral inclusions and associated lesions in numerous tissues, adenoviral intranuclear inclusions in bronchi…
View article: Natural Canine Distemper Virus Infection in Linnaeus’s 2-Toed Sloths (<i>Choloepus didactylus</i>)
Natural Canine Distemper Virus Infection in Linnaeus’s 2-Toed Sloths (<i>Choloepus didactylus</i>) Open
An outbreak of canine distemper virus in a private zoo in eastern Tennessee in July 2016 led to fatal clinical disease in 5 adult, wild-caught Linnaeus’s 2-toed sloths ( Choloepus didactylus). Clinical signs included hyporexia, lethargy, m…
View article: Dual infection with an emergent strain of canine distemper virus and canine parvovirus in an Arctic wolf under managed care
Dual infection with an emergent strain of canine distemper virus and canine parvovirus in an Arctic wolf under managed care Open
A 6-wk-old managed male Arctic wolf with lethargy, drooling, dehydration, elevated temperature, and acute onset of seizures was submitted for autopsy. The wolf had been vaccinated with a multivalent vaccine exactly 2 wk prior to presentati…
View article: Infection of eight mesocarnivores in New Hampshire and Vermont with a distinct clade of canine distemper virus in 2016–2017
Infection of eight mesocarnivores in New Hampshire and Vermont with a distinct clade of canine distemper virus in 2016–2017 Open
Three fishers ( Martes pennanti), 2 gray foxes ( Urocyon cinereoargenteus), 1 mink ( Neovison vison), 1 skunk ( Mephitis mephitis), and 1 raccoon ( Procyon lotor), from Vermont and New Hampshire, had lesions on autopsy consistent with cani…