Emanuele Andreano
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View article: Antigen-agnostic identification of poxvirus broadly neutralizing antibodies targeting OPG153
Antigen-agnostic identification of poxvirus broadly neutralizing antibodies targeting OPG153 Open
Recurrent mpox outbreaks caused by the monkeypox virus (MPXV) have prompted the World Health Organization to declare a Public Health Emergency of International Concern and have stimulated the development of medical interventions. Here, ant…
View article: A multifunctional anti-O-Antigen human monoclonal antibody protects against <i>Shigella sonnei</i> infection in vivo
A multifunctional anti-O-Antigen human monoclonal antibody protects against <i>Shigella sonnei</i> infection in vivo Open
Shigellosis is a global public health challenge that mostly affects low- and middle-income countries and causes considerable morbidity and mortality among children under 5 y of age. Multi- and extensively drug-resistant Shigella sonnei str…
View article: Antigenic sin and multiple breakthrough infections drive converging evolution of COVID-19 neutralizing responses
Antigenic sin and multiple breakthrough infections drive converging evolution of COVID-19 neutralizing responses Open
Understanding the evolution of the B cell response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants is fundamental to design the next generation of vaccines and therapeutics. We longitudinally analyze at the single-…
View article: SARS-CoV-2 JN.1 variant evasion of IGHV3-53/3-66 B cell germlines
SARS-CoV-2 JN.1 variant evasion of IGHV3-53/3-66 B cell germlines Open
The severe acute respiratory syndrome coronavirus 2 variant JN.1 recently emerged as the dominant variant despite having only one amino acid change on the spike (S) protein receptor binding domain (RBD) compared with the ancestral BA.2.86,…
View article: Functional diversification of innate and inflammatory immune responses mediated by antibody fragment crystallizable activities against SARS-CoV-2
Functional diversification of innate and inflammatory immune responses mediated by antibody fragment crystallizable activities against SARS-CoV-2 Open
Monoclonal antibodies (mAb) targeting the SARS-CoV-2 Spike (S) glycoprotein have been exploited for the treatment of severe COVID-19. In this study, we evaluated the immune-regulatory features of two neutralizing anti-S mAbs (nAbs), named …
View article: B cell maturation restored ancestral germlines to control Omicron BA.2.86
B cell maturation restored ancestral germlines to control Omicron BA.2.86 Open
The unceasing interplay between SARS-CoV-2 and the human immune system has led to a continuous maturation of the virus and B cell response providing an opportunity to track their evolution in real time. We longitudinally analyzed the funct…
View article: Deep-learning image analysis for high-throughput screening of opsono-phagocytosis-promoting monoclonal antibodies against Neisseria gonorrhoeae
Deep-learning image analysis for high-throughput screening of opsono-phagocytosis-promoting monoclonal antibodies against Neisseria gonorrhoeae Open
Antimicrobial resistance (AMR) is nowadays a global health concern as bacterial pathogens are increasingly developing resistance to antibiotics. Monoclonal antibodies (mAbs) represent a powerful tool for addressing AMR thanks to their high…
View article: Epitope Mapping of Human Polyclonal Antibodies to the fHbp Antigen of a Neisseria Meningitidis Vaccine by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS)
Epitope Mapping of Human Polyclonal Antibodies to the fHbp Antigen of a Neisseria Meningitidis Vaccine by Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Open
Antigen-antibody interactions play a key role in the immune response post vaccination and the mechanism of action of antibody-based biopharmaceuticals. 4CMenB is a multicomponent vaccine against Neisseria meningitidis serogroup B in which …
View article: Discovery and Characterization of a Pan-betacoronavirus S2-binding antibody
Discovery and Characterization of a Pan-betacoronavirus S2-binding antibody Open
SUMMARY/ABSTRACT Three coronaviruses have spilled over from animal reservoirs into the human population and caused deadly epidemics or pandemics. The continued emergence of coronaviruses highlights the need for pan-coronavirus intervention…
View article: High-resolution map of the Fc functions mediated by COVID-19-neutralizing antibodies
High-resolution map of the Fc functions mediated by COVID-19-neutralizing antibodies Open
A growing body of evidence shows that fragment crystallizable (Fc)-dependent antibody effector functions play an important role in protection from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. To unravel the mecha…
View article: Human monoclonal antibodies reveal subdominant gonococcal and meningococcal cross-protective antigens
Human monoclonal antibodies reveal subdominant gonococcal and meningococcal cross-protective antigens Open
Gonococcus (Gc), a bacterium resistant to most antibiotics causing more than 80 million cases of gonorrhea annually, is a WHO high priority pathogen. Recently, vaccine development prospects were boosted by reports that licensed meningococc…
View article: Prior SARS-CoV-2 Infection Enhances Initial mRNA Vaccine Response with a Lower Impact on Long-Term Immunity
Prior SARS-CoV-2 Infection Enhances Initial mRNA Vaccine Response with a Lower Impact on Long-Term Immunity Open
Spike-encoding mRNA vaccines in early 2021 effectively reduced SARS-CoV-2–associated morbidity and mortality. New booster regimens were introduced due to successive waves of distinct viral variants. Therefore, people now have a diverse imm…
View article: High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae
High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae Open
Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a signif…
View article: High-resolution map of the Fc-functions mediated by COVID-19 neutralizing antibodies
High-resolution map of the Fc-functions mediated by COVID-19 neutralizing antibodies Open
A growing body of evidence shows that Fc-dependent antibody effector functions play an important role in protection from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. To unravel the mechanisms that drive these res…
View article: High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant<i>Neisseria gonorrhoeae</i>
High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant<i>Neisseria gonorrhoeae</i> Open
Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a signif…
View article: Emerging roles of SARS-CoV-2 Spike-ACE2 in immune evasion and pathogenesis
Emerging roles of SARS-CoV-2 Spike-ACE2 in immune evasion and pathogenesis Open
The COVID-19 pandemic, caused by SARS-CoV-2, has caused an estimated 5 billion infections and 20 million deaths by respiratory failure. In addition to the respiratory disease, SARS-CoV-2 infection has been associated with many extrapulmona…
View article: mRNA vaccines and hybrid immunity use different B cell germlines against Omicron BA.4 and BA.5
mRNA vaccines and hybrid immunity use different B cell germlines against Omicron BA.4 and BA.5 Open
Severe acute respiratory syndrome 2 Omicron BA.4 and BA.5 are characterized by high transmissibility and ability to escape natural and vaccine induced immunity. Here we test the neutralizing activity of 482 human monoclonal antibodies isol…
View article: B cell analyses after SARS-CoV-2 mRNA third vaccination reveals a hybrid immunity like antibody response
B cell analyses after SARS-CoV-2 mRNA third vaccination reveals a hybrid immunity like antibody response Open
View article: A new dawn for monoclonal antibodies against antimicrobial resistant bacteria
A new dawn for monoclonal antibodies against antimicrobial resistant bacteria Open
Antimicrobial resistance (AMR) is a quickly advancing threat for human health worldwide and almost 5 million deaths are already attributable to this phenomenon every year. Since antibiotics are failing to treat AMR-bacteria, new tools are …
View article: SARS-CoV-2 Spike protein suppresses CTL-mediated killing by inhibiting immune synapse assembly
SARS-CoV-2 Spike protein suppresses CTL-mediated killing by inhibiting immune synapse assembly Open
CTL-mediated killing of virally infected or malignant cells is orchestrated at the immune synapse (IS). We hypothesized that SARS-CoV-2 may target lytic IS assembly to escape elimination. We show that human CD8+ T cells upregulate the expr…
View article: The neutralizing response to SARS-CoV-2 Omicron variants BA.1 and BA.2 in COVID-19 patients and homologous and heterologous vaccinees
The neutralizing response to SARS-CoV-2 Omicron variants BA.1 and BA.2 in COVID-19 patients and homologous and heterologous vaccinees Open
The rapid replacement of Omicron BA.1 by BA.2 sublineage is very alarming, raising the question of whether BA.2 can escape the immunity acquired after BA.1 infection. We compared the neutralizing activity toward the Omicron BA.1 and BA.2 s…
View article: 2D NMR Analysis as a Sensitive Tool for Evaluating the Higher-Order Structural Integrity of Monoclonal Antibody against COVID-19
2D NMR Analysis as a Sensitive Tool for Evaluating the Higher-Order Structural Integrity of Monoclonal Antibody against COVID-19 Open
The higher-order structure (HOS) of protein therapeutics has been confirmed as a critical quality parameter. In this study, we compared 2D 1H-13C ALSOFAST-HMQC NMR spectra with immunochemical ELISA-based analysis to evaluate their sensitiv…
View article: Production of two SARS-CoV-2 neutralizing antibodies with different potencies in Nicotiana benthamiana
Production of two SARS-CoV-2 neutralizing antibodies with different potencies in Nicotiana benthamiana Open
Monoclonal antibodies are considered to be highly effective therapeutic tools for the treatment of mild to moderate COVID-19 patients. In the present work, we describe the production of two SARS-CoV-2 human IgG1 monoclonal antibodies recog…
View article: Immune response to SARS-CoV-2 Omicron variant in patients and vaccinees following homologous and heterologous vaccinations
Immune response to SARS-CoV-2 Omicron variant in patients and vaccinees following homologous and heterologous vaccinations Open
View article: mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5
mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5 Open
SARS-CoV-2 omicron BA.4 and BA.5, characterized by high transmissibility and ability to escape natural and vaccine induced immunity, are rampaging worldwide. To understand the escape mechanisms, we tested the neutralizing activity against …
View article: Anatomy of Omicron BA.1 and BA.2 neutralizing antibodies in COVID-19 mRNA vaccinees
Anatomy of Omicron BA.1 and BA.2 neutralizing antibodies in COVID-19 mRNA vaccinees Open
SARS-CoV-2 vaccines, administered to billions of people worldwide, mitigate the effects of the COVID-19 pandemic, however little is known about the molecular basis of antibody cross-protection to emerging variants, such as Omicron BA.1, it…
View article: SARS-CoV-2 Spike protein suppresses CTL-mediated killing by inhibiting immune synapse assembly
SARS-CoV-2 Spike protein suppresses CTL-mediated killing by inhibiting immune synapse assembly Open
CTL-mediated killing of virally infected or malignant cells is orchestrated at a specialized intercellular junction, the immune synapse (IS). We hypothesized that SARS-CoV-2 may target IS assembly in CTLs to escape killing. We show that pr…
View article: Immune response in COVID-19: what is next?
Immune response in COVID-19: what is next? Open
The coronavirus disease 2019 (COVID-19) has been a global pandemic for more than 2 years and it still impacts our daily lifestyle and quality in unprecedented ways. A better understanding of immunity and its regulation in response to SARS-…
View article: Structural insights of a highly potent pan-neutralizing SARS-CoV-2 human monoclonal antibody
Structural insights of a highly potent pan-neutralizing SARS-CoV-2 human monoclonal antibody Open
Significance Clinical candidate monoclonal antibody J08 binds the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) S-protein independent of known escape mutations and is able to potently neutralize most variants of concern (VoC…
View article: COVID-19 mRNA third dose induces a unique hybrid immunity-like antibody response
COVID-19 mRNA third dose induces a unique hybrid immunity-like antibody response Open
The continuous evolution of SARS-CoV-2 generated highly mutated variants, like omicron BA.1 and BA.2, able to escape natural and vaccine-induced primary immunity 1,2 . The administration of a third dose of mRNA vaccines induces a secondary…